Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 1 cut(s) | 261 |
| AcsI | RAATTY | 2 cut(s) | 22, 338 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 242, 395 |
| AgsI | TTSAA | 3 cut(s) | 273, 308, 335 |
| AjnI | CCWGG | 2 cut(s) | 200, 394 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 48, 80 |
| AluBI | AGCT | 1 cut(s) | 194 |
| AluI | AGCT | 1 cut(s) | 194 |
| ApeKI | GCWGC | 1 cut(s) | 97 |
| ApoI | RAATTY | 2 cut(s) | 22, 338 |
| AsuHPI | GGTGA | 1 cut(s) | 199 |
| BanII | GRGCYC | 1 cut(s) | 268 |
| BbvI | GCAGC | 1 cut(s) | 109 |
| BccI | CCATC | 2 cut(s) | 97, 314 |
| BciT130I | CCWGG | 2 cut(s) | 202, 396 |
| BfaI | CTAG | 1 cut(s) | 143 |
| BisI | GCNGC | 1 cut(s) | 98 |
| BlsI | GCNGC | 1 cut(s) | 99 |
| Bme1390I | CCNGG | 2 cut(s) | 202, 396 |
| BmrFI | CCNGG | 2 cut(s) | 202, 396 |
| BpmI | CTGGAG | 1 cut(s) | 223 |
| BsaBI | GATNNNNATC | 1 cut(s) | 8 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 48, 78, 196, 226 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 242, 395 |
| Bse3DI | GCAATG | 2 cut(s) | 41, 133 |
| Bse8I | GATNNNNATC | 1 cut(s) | 8 |
| BseBI | CCWGG | 2 cut(s) | 202, 396 |
| BseGI | GGATG | 3 cut(s) | 163, 169, 325 |
| BseJI | GATNNNNATC | 1 cut(s) | 8 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 242, 395 |
| BseMI | GCAATG | 2 cut(s) | 41, 133 |
| BseXI | GCAGC | 1 cut(s) | 109 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 242, 395 |
| Bsp1286I | GDGCHC | 1 cut(s) | 268 |
| BspACI | CCGC | 1 cut(s) | 261 |
| BsrDI | GCAATG | 2 cut(s) | 41, 133 |
| Bst2UI | CCWGG | 2 cut(s) | 202, 396 |
| Bst6I | CTCTTC | 1 cut(s) | 366 |
| BstF5I | GGATG | 3 cut(s) | 163, 169, 325 |
| BstNI | CCWGG | 2 cut(s) | 202, 396 |
| BstSCI | CCNGG | 2 cut(s) | 200, 394 |
| BstV1I | GCAGC | 1 cut(s) | 109 |
| BtsCI | GGATG | 3 cut(s) | 163, 169, 325 |
| BtsI | GCAGTG | 1 cut(s) | 256 |
| BtsIMutI | CAGTG | 1 cut(s) | 256 |
| CseI | GACGC | 1 cut(s) | 287 |
| CviJI | RGCY | 6 cut(s) | 119, 133, 194, 219, 246, 266 |
| CviKI_1 | RGCY | 6 cut(s) | 119, 133, 194, 219, 246, 266 |
| Eam1104I | CTCTTC | 1 cut(s) | 366 |
| EarI | CTCTTC | 1 cut(s) | 366 |
| Eco24I | GRGCYC | 1 cut(s) | 268 |
| EcoRI | GAATTC | 2 cut(s) | 22, 338 |
| EcoRII | CCWGG | 2 cut(s) | 200, 394 |
| EcoT38I | GRGCYC | 1 cut(s) | 268 |
| FaiI | YATR | 5 cut(s) | 14, 54, 312, 329, 351 |
| FauI | CCCGC | 1 cut(s) | 254 |
| Fnu4HI | GCNGC | 1 cut(s) | 98 |
| FokI | GGATG | 3 cut(s) | 170, 176, 332 |
| FriOI | GRGCYC | 1 cut(s) | 268 |
| Fsp4HI | GCNGC | 1 cut(s) | 98 |
| FspBI | CTAG | 1 cut(s) | 143 |
| GluI | GCNGC | 1 cut(s) | 98 |
| GsuI | CTGGAG | 1 cut(s) | 223 |
| HgaI | GACGC | 1 cut(s) | 287 |
| HincII | GTYRAC | 2 cut(s) | 169, 298 |
| HindII | GTYRAC | 2 cut(s) | 169, 298 |
| HinfI | GANTC | 1 cut(s) | 4 |
| HpaI | GTTAAC | 1 cut(s) | 298 |
| HphI | GGTGA | 1 cut(s) | 199 |
| Hpy166II | GTNNAC | 2 cut(s) | 169, 298 |
| Hpy188I | TCNGA | 2 cut(s) | 74, 94 |
| Hpy188III | TCNNGA | 1 cut(s) | 143 |
| Hpy8I | GTNNAC | 2 cut(s) | 169, 298 |
| HpyCH4V | TGCA | 2 cut(s) | 34, 126 |
| KspAI | GTTAAC | 1 cut(s) | 298 |
| LmnI | GCTCC | 1 cut(s) | 204 |
| LpnPI | CCDG | 8 cut(s) | 3, 119, 187, 214, 232, 250, 381, 402 |
| Lsp1109I | GCAGC | 1 cut(s) | 109 |
| MaeI | CTAG | 1 cut(s) | 143 |
| MaeIII | GTNAC | 1 cut(s) | 375 |
| MboII | GAAGA | 4 cut(s) | 166, 172, 347, 383 |
| MfeI | CAATTG | 1 cut(s) | 29 |
| MhlI | GDGCHC | 1 cut(s) | 268 |
| MluCI | AATT | 6 cut(s) | 22, 29, 108, 138, 240, 338 |
| MnlI | CCTC | 2 cut(s) | 139, 246 |
| MseI | TTAA | 1 cut(s) | 297 |
| MspR9I | CCNGG | 2 cut(s) | 202, 396 |
| MunI | CAATTG | 1 cut(s) | 29 |
| MvaI | CCWGG | 2 cut(s) | 202, 396 |
| PfeI | GAWTC | 1 cut(s) | 4 |
| PfoI | TCCNGGA | 1 cut(s) | 200 |
| PkrI | GCNGC | 1 cut(s) | 99 |
| Psp6I | CCWGG | 2 cut(s) | 200, 394 |
| PspGI | CCWGG | 2 cut(s) | 200, 394 |
| SaqAI | TTAA | 1 cut(s) | 297 |
| SatI | GCNGC | 1 cut(s) | 98 |
| ScrFI | CCNGG | 2 cut(s) | 202, 396 |
| SduI | GDGCHC | 1 cut(s) | 268 |
| SetI | ASST | 2 cut(s) | 150, 196 |
| Sse9I | AATT | 6 cut(s) | 22, 29, 108, 138, 240, 338 |
| SsiI | CCGC | 1 cut(s) | 261 |
| SspMI | CTAG | 1 cut(s) | 143 |
| StyD4I | CCNGG | 2 cut(s) | 200, 394 |
| TasI | AATT | 6 cut(s) | 22, 29, 108, 138, 240, 338 |
| TfiI | GAWTC | 1 cut(s) | 4 |
| Tru1I | TTAA | 1 cut(s) | 297 |
| Tru9I | TTAA | 1 cut(s) | 297 |
| TscAI | CASTG | 1 cut(s) | 263 |
| TseI | GCWGC | 1 cut(s) | 97 |
| TspDTI | ATGAA | 1 cut(s) | 166 |
| TspRI | CASTG | 1 cut(s) | 263 |
| XapI | RAATTY | 2 cut(s) | 22, 338 |
| XbaI | TCTAGA | 1 cut(s) | 142 |
| XspI | CTAG | 1 cut(s) | 143 |