Rw6G006220

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000144
Physical Location & Seq
Forward (+)
9831595 .. 9833172
1578 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G006220.1

Sequence Viewer

Length: 1578 bp
ATGAAGATGATAAGAAACCAAACCAGTTCTAATGAATGTACCTCTTGGCAGCCACCACCTCCTGACATCATGGAGCAAGTTCTGCCGCGTGTCAGAGTAAGTGATCAAATAAGGCTAAGCATTGCCTGCAAGTCTTGGATGTCAATCGTTATGCGTAGAGACATCCGCAGTGCTTCTCATGAATTCCCATGGTTACTACTCCCTCAAACCCCACATTGCAGCAACAAGTACGTAAGCTTTGCCAGCGTGTCAGAGGGGAGAGTTGTCAAGTTGAAGCTACCTAAGCAAGTTCGTGGATGGTGGATGTTTGGGTCTTCTAAAGGTTGGTTGATCATGATCAAGGAAAGAGGTCTAAATTCCAAGATGTGTCTACTTAACCCAATTTCAGGAGCCCTACTCCAACTTCCACCCTTGAGAACACTTCCTTTTCTGAAAGATTTTGTGAAAACCGAGGACTGGAAACTTTTTGGTGCCAATGCATTCGACTTAAGGATTGCACTATCAACCTCTGATGGAATTGCTTTAGATTCAAAGCGTTGTACGGTAGCAGCAGTTTTTAATGATAAGTCGAAATTGAGTTTGTGCAGACCTGGAGACAGAACATGGAGTGACTTTCAAGTATTAGATACTAATCAAAACGACTGGATTGCTGATTTATTGTTTTCTTCCGGCAGTCTATATGTTTTGGTTAGTGGTGGTCAAAAGGAAAGCTTTGTAGACTCAGTTACTCAAACCTTGAACTTTAGCTTTGGAGATGCAGAAAATTTGAAAATGAAGTTGGTCTACGACAAGCATGAAAACAGGAACGTGAACGTTAATGAATGTCATAGTGACTATAAGATCGTTTACAATGCATTATACTTCTCAAGATTGTTAGAATCAACCAGCAATGAAGTCTTATTGATCCATCAAATGGTAGATTATGTTGTGGGAAGAACAGAGGATATTATTGGTGACAATAATGAGAATAATGGAGGCGGCAATATGGAGAATGATGAGGATGATGATGAAGCCAACAACTTAGAAGATATTGATCAGCAAAATGGAGGAAACATTATTTATGGTGATGAAGGCAGCATTAGTGGCGACAGCAATATGGAAAATGATGATGGTGGTGAAGTCCATAACGACAATAATGATGTTGATGTGGGAGACATTAATCAGAATCCTGAAGGTGATGAGGAAGACAACAATAACCCAGAAGATAATGGTCAGCAAGGAGAAACTATTGATGAAGATGCACCGGGATTTAATGCATATATGACAACAAGGAGTTTTAGAGTATACAAGACTGACGACGACAACTTTATTCCATTGCATTGCTTGGGGGATCAATTATTCTTTCTTGGAGATTGTGGTTCCTTCTCCTTTGGAGTCAGTAATATGAAAAAATCAGAAAGGAATTGTATTTATTTTGCATCAAATTTGATGTACGATTGGGAGGCAGCTCCAAAAACGTATGCATCTCGTGACATTGGTATATTCTACCTAGATAGCCAAAGAATTGAGCGGTCATTTCCAAGTGTTGAGATGTCTCTACGGTATCAAGGGACTTGGTTCACTCCAAGTTTATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

525

Amino Acids

59.45

Weight (kDa)

4.61

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 80 - 304 8.7e-21 KIB1-4 beta-propeller
Beta-prop_KIB1-4 PF03478 420 - 495 4.5e-09 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 470
AccB7I CCANNNNNTGG 1 cut(s) 913
AccBSI CCGCTC 1 cut(s) 1510
AccI GTMKAC 4 cut(s) 370, 717, 783, 1284
AccII CGCG 1 cut(s) 88
AciI CCGC 4 cut(s) 86, 166, 978, 1510
AclI AACGTT 1 cut(s) 813
AclWI GGATC 2 cut(s) 898, 1338
AcsI RAATTY 4 cut(s) 182, 355, 763, 1423
AcuI CTGAAG 1 cut(s) 1191
AfaI GTAC 4 cut(s) 40, 230, 541, 1433
AfiI CCNNNNNNNGG 3 cut(s) 386, 456, 913
AflII CTTAAG 1 cut(s) 487
AgsI TTSAA 5 cut(s) 274, 531, 617, 739, 769
AjnI CCWGG 1 cut(s) 589
AjuI GAANNNNNNNTTGG 2 cut(s) 761, 793
AluBI AGCT 5 cut(s) 237, 277, 711, 747, 1448
AluI AGCT 5 cut(s) 237, 277, 711, 747, 1448
Alw26I GTCTC 4 cut(s) 153, 588, 1146, 1539
AlwI GGATC 2 cut(s) 898, 1338
ApeKI GCWGC 5 cut(s) 49, 219, 548, 1074, 1445
ApoI RAATTY 4 cut(s) 182, 355, 763, 1423
AseI ATTAAT 1 cut(s) 1158
AsuC2I CCSGG 1 cut(s) 1245
AsuHPI GGTGA 4 cut(s) 965, 1076, 1127, 1187
BanI GGYRCC 1 cut(s) 470
BanII GRGCYC 1 cut(s) 394
BarI GAAGNNNNNNTAC 2 cut(s) 767, 799
BauI CACGAG 1 cut(s) 1467
BbsI GAAGAC 2 cut(s) 306, 1191
BbvI GCAGC 5 cut(s) 61, 231, 560, 1086, 1457
BccI CCATC 4 cut(s) 291, 506, 915, 1103
BcgI CGANNNNNNTGC 2 cut(s) 629, 663
BciT130I CCWGG 1 cut(s) 591
BclI TGATCA 4 cut(s) 103, 330, 336, 1033
BcnI CCSGG 1 cut(s) 1245
BcoDI GTCTC 4 cut(s) 153, 588, 1146, 1539
BfaI CTAG 1 cut(s) 1490
BfrI CTTAAG 1 cut(s) 487
BisI GCNGC 7 cut(s) 50, 86, 220, 549, 979, 1075, 1446
BlpI GCTNAGC 1 cut(s) 116
BlsI GCNGC 7 cut(s) 51, 87, 221, 550, 980, 1076, 1447
Bme1390I CCNGG 2 cut(s) 591, 1245
BmiI GGNNCC 3 cut(s) 391, 472, 1360
BmrFI CCNGG 2 cut(s) 591, 1245
BmsI GCATC 4 cut(s) 745, 1228, 1427, 1472
BpiI GAAGAC 2 cut(s) 306, 1191
BplI GAGNNNNNCTC 2 cut(s) 381, 413
BpmI CTGGAG 1 cut(s) 612
Bpu10I CCTNAGC 1 cut(s) 282
Bpu1102I GCTNAGC 1 cut(s) 116
BpuEI CTTGAG 2 cut(s) 433, 850
BpuMI CCSGG 1 cut(s) 1245
BsaAI YACGTR 1 cut(s) 232
BsaBI GATNNNNATC 4 cut(s) 143, 335, 630, 1032
BsaJI CCNNGG 2 cut(s) 188, 450
Bsc4I CCNNNNNNNGG 3 cut(s) 386, 456, 913
Bse1I ACTGG 3 cut(s) 24, 461, 647
Bse3DI GCAATG 5 cut(s) 120, 214, 895, 1313, 1318
Bse8I GATNNNNATC 4 cut(s) 143, 335, 630, 1032
BseBI CCWGG 1 cut(s) 591
BseDI CCNNGG 2 cut(s) 188, 450
BseGI GGATG 5 cut(s) 144, 162, 302, 309, 1006
BseJI GATNNNNATC 4 cut(s) 143, 335, 630, 1032
BseLI CCNNNNNNNGG 3 cut(s) 386, 456, 913
BseMI GCAATG 5 cut(s) 120, 214, 895, 1313, 1318
BseMII CTCAG 1 cut(s) 735
BseNI ACTGG 3 cut(s) 24, 461, 647
BseXI GCAGC 5 cut(s) 61, 231, 560, 1086, 1457
BsgI GTGCAG 1 cut(s) 604
Bsh1236I CGCG 1 cut(s) 88
BshNI GGYRCC 1 cut(s) 470
BsiSI CCGG 2 cut(s) 669, 1244
BslFI GGGAC 1 cut(s) 1564
BslI CCNNNNNNNGG 3 cut(s) 386, 456, 913
BsmAI GTCTC 4 cut(s) 153, 588, 1146, 1539
BsmFI GGGAC 1 cut(s) 1564
BsmI GAATGC 1 cut(s) 479
Bsp1286I GDGCHC 1 cut(s) 394
Bsp143I GATC 7 cut(s) 103, 330, 336, 840, 903, 1033, 1330
Bsp1720I GCTNAGC 1 cut(s) 116
Bsp19I CCATGG 1 cut(s) 188
BspACI CCGC 4 cut(s) 86, 166, 978, 1510
BspCNI CTCAG 1 cut(s) 734
BspFNI CGCG 1 cut(s) 88
BspHI TCATGA 2 cut(s) 178, 333
BspLI GGNNCC 3 cut(s) 391, 472, 1360
BspPI GGATC 2 cut(s) 898, 1338
BspT107I GGYRCC 1 cut(s) 470
BspTI CTTAAG 1 cut(s) 487
BsrBI CCGCTC 1 cut(s) 1510
BsrDI GCAATG 5 cut(s) 120, 214, 895, 1313, 1318
BsrI ACTGG 3 cut(s) 24, 461, 647
BssECI CCNNGG 2 cut(s) 188, 450
BssMI GATC 7 cut(s) 103, 330, 336, 840, 903, 1033, 1330
BssNAI GTATAC 1 cut(s) 1285
BssSI CACGAG 1 cut(s) 1467
BssT1I CCWWGG 1 cut(s) 188
Bst1107I GTATAC 1 cut(s) 1285
Bst2BI CACGAG 1 cut(s) 1467
Bst2UI CCWGG 1 cut(s) 591
Bst4CI ACNGT 2 cut(s) 544, 1542
BstAFI CTTAAG 1 cut(s) 487
BstAPI GCANNNNNTGC 2 cut(s) 82, 126
BstBAI YACGTR 1 cut(s) 232
BstC8I GCNNGC 2 cut(s) 127, 244
BstDEI CTNAG 4 cut(s) 116, 282, 721, 1021
BstDSI CCRYGG 1 cut(s) 188
BstF5I GGATG 5 cut(s) 144, 162, 302, 309, 1006
BstFNI CGCG 1 cut(s) 88
BstKTI GATC 7 cut(s) 106, 333, 339, 843, 906, 1036, 1333
BstMAI GTCTC 4 cut(s) 153, 588, 1146, 1539
BstMBI GATC 7 cut(s) 103, 330, 336, 840, 903, 1033, 1330
BstMWI GCNNNNNNNGC 5 cut(s) 82, 126, 243, 283, 1083
BstNI CCWGG 1 cut(s) 591
BstSCI CCNGG 2 cut(s) 589, 1243
BstSNI TACGTA 1 cut(s) 232
BstUI CGCG 1 cut(s) 88
BstV1I GCAGC 5 cut(s) 61, 231, 560, 1086, 1457
BstV2I GAAGAC 2 cut(s) 306, 1191
BstZ17I GTATAC 1 cut(s) 1285
BtgI CCRYGG 1 cut(s) 188
BtsCI GGATG 5 cut(s) 144, 162, 302, 309, 1006
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 1 cut(s) 175
Cac8I GCNNGC 2 cut(s) 127, 244
CciI TCATGA 2 cut(s) 178, 333
Csp6I GTAC 4 cut(s) 39, 229, 540, 1432
CviAII CATG 6 cut(s) 70, 179, 189, 334, 603, 794
CviQI GTAC 4 cut(s) 39, 229, 540, 1432
DdeI CTNAG 4 cut(s) 116, 282, 721, 1021
DpnI GATC 7 cut(s) 105, 332, 338, 842, 905, 1035, 1332
DpnII GATC 7 cut(s) 103, 330, 336, 840, 903, 1033, 1330
Eco105I TACGTA 1 cut(s) 232
Eco130I CCWWGG 1 cut(s) 188
Eco24I GRGCYC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 1191
EcoRI GAATTC 1 cut(s) 182
EcoRII CCWGG 1 cut(s) 589
EcoT14I CCWWGG 1 cut(s) 188
EcoT22I ATGCAT 4 cut(s) 481, 856, 1258, 1465
EcoT38I GRGCYC 1 cut(s) 394
ErhI CCWWGG 1 cut(s) 188
FaeI CATG 6 cut(s) 73, 182, 192, 337, 606, 797
FalI AAGNNNNNCTT 2 cut(s) 695, 727
FaqI GGGAC 1 cut(s) 1564
FatI CATG 6 cut(s) 69, 178, 188, 333, 602, 793
FbaI TGATCA 4 cut(s) 103, 330, 336, 1033
FblI GTMKAC 4 cut(s) 370, 717, 783, 1284
Fnu4HI GCNGC 7 cut(s) 50, 86, 220, 549, 979, 1075, 1446
FokI GGATG 5 cut(s) 149, 151, 309, 316, 1013
FriOI GRGCYC 1 cut(s) 394
Fsp4HI GCNGC 7 cut(s) 50, 86, 220, 549, 979, 1075, 1446
FspBI CTAG 1 cut(s) 1490
GluI GCNGC 7 cut(s) 50, 86, 220, 549, 979, 1075, 1446
GsuI CTGGAG 1 cut(s) 612
HapII CCGG 2 cut(s) 669, 1244
Hin1II CATG 6 cut(s) 73, 182, 192, 337, 606, 797
HindIII AAGCTT 2 cut(s) 235, 709
HinfI GANTC 5 cut(s) 527, 719, 878, 1165, 1374
HpaII CCGG 2 cut(s) 669, 1244
HphI GGTGA 4 cut(s) 965, 1076, 1127, 1187
Hpy166II GTNNAC 7 cut(s) 371, 718, 784, 811, 847, 1285, 1560
Hpy188I TCNGA 6 cut(s) 95, 253, 432, 511, 1164, 1396
Hpy188III TCNNGA 7 cut(s) 62, 179, 334, 387, 867, 1169, 1469
Hpy8I GTNNAC 7 cut(s) 371, 718, 784, 811, 847, 1285, 1560
Hpy99I CGWCG 1 cut(s) 1301
HpyAV CCTTC 3 cut(s) 1064, 1166, 1372
HpyCH4III ACNGT 2 cut(s) 544, 1542
HpyCH4IV ACGT 4 cut(s) 231, 807, 813, 1457
HpyF10VI GCNNNNNNNGC 5 cut(s) 82, 126, 243, 283, 1083
HpyF3I CTNAG 4 cut(s) 116, 282, 721, 1021
HpySE526I ACGT 4 cut(s) 231, 807, 813, 1457
Hsp92II CATG 6 cut(s) 73, 182, 192, 337, 606, 797
Ksp22I TGATCA 4 cut(s) 103, 330, 336, 1033
Kzo9I GATC 7 cut(s) 103, 330, 336, 840, 903, 1033, 1330
LmnI GCTCC 3 cut(s) 73, 389, 1453
Lsp1109I GCAGC 5 cut(s) 61, 231, 560, 1086, 1457
LweI GCATC 4 cut(s) 745, 1228, 1427, 1472
MaeI CTAG 1 cut(s) 1490
MaeII ACGT 4 cut(s) 231, 807, 813, 1457
MaeIII GTNAC 6 cut(s) 192, 608, 724, 830, 953, 1469
MalI GATC 7 cut(s) 105, 332, 338, 842, 905, 1035, 1332
MbiI CCGCTC 1 cut(s) 1510
MboI GATC 7 cut(s) 103, 330, 336, 840, 903, 1033, 1330
MboII GAAGA 8 cut(s) 16, 306, 657, 945, 1037, 1196, 1214, 1247
MhlI GDGCHC 1 cut(s) 394
MlyI GAGTC 2 cut(s) 713, 1383
MmeI TCCRAC 1 cut(s) 424
Mph1103I ATGCAT 4 cut(s) 481, 856, 1258, 1465
MseI TTAA 6 cut(s) 375, 488, 558, 816, 1158, 1251
MspCI CTTAAG 1 cut(s) 487
MspI CCGG 2 cut(s) 669, 1244
MspR9I CCNGG 2 cut(s) 591, 1245
Mva1269I GAATGC 1 cut(s) 479
MvaI CCWGG 1 cut(s) 591
MvnI CGCG 1 cut(s) 88
MwoI GCNNNNNNNGC 5 cut(s) 82, 126, 243, 283, 1083
NciI CCSGG 1 cut(s) 1245
NcoI CCATGG 1 cut(s) 188
NdeII GATC 7 cut(s) 103, 330, 336, 840, 903, 1033, 1330
NlaIII CATG 6 cut(s) 73, 182, 192, 337, 606, 797
NlaIV GGNNCC 3 cut(s) 391, 472, 1360
NmuCI GTSAC 4 cut(s) 608, 830, 953, 1469
NsiI ATGCAT 4 cut(s) 481, 856, 1258, 1465
PagI TCATGA 2 cut(s) 178, 333
PctI GAATGC 1 cut(s) 479
PfeI GAWTC 3 cut(s) 527, 878, 1165
PflMI CCANNNNNTGG 1 cut(s) 913
PkrI GCNGC 7 cut(s) 51, 87, 221, 550, 980, 1076, 1447
PleI GAGTC 2 cut(s) 713, 1382
PpsI GAGTC 2 cut(s) 713, 1382
Ppu21I YACGTR 1 cut(s) 232
PshBI ATTAAT 1 cut(s) 1158
Psp1406I AACGTT 1 cut(s) 813
Psp6I CCWGG 1 cut(s) 589
PspGI CCWGG 1 cut(s) 589
PspN4I GGNNCC 3 cut(s) 391, 472, 1360
RsaI GTAC 4 cut(s) 40, 230, 541, 1433
RsaNI GTAC 4 cut(s) 39, 229, 540, 1432
SaqAI TTAA 6 cut(s) 375, 488, 558, 816, 1158, 1251
SatI GCNGC 7 cut(s) 50, 86, 220, 549, 979, 1075, 1446
Sau3AI GATC 7 cut(s) 103, 330, 336, 840, 903, 1033, 1330
SchI GAGTC 2 cut(s) 713, 1383
ScrFI CCNGG 2 cut(s) 591, 1245
SduI GDGCHC 1 cut(s) 394
SfaNI GCATC 4 cut(s) 745, 1228, 1427, 1472
SmlI CTYRAG 3 cut(s) 412, 487, 865
SmoI CTYRAG 3 cut(s) 412, 487, 865
SnaBI TACGTA 1 cut(s) 232
SsiI CCGC 4 cut(s) 86, 166, 978, 1510
SspMI CTAG 1 cut(s) 1490
StyD4I CCNGG 2 cut(s) 589, 1243
StyI CCWWGG 1 cut(s) 188
TaaI ACNGT 2 cut(s) 544, 1542
TaiI ACGT 4 cut(s) 234, 810, 816, 1460
TaqI TCGA 2 cut(s) 483, 569
TauI GCSGC 2 cut(s) 88, 981
TfiI GAWTC 3 cut(s) 527, 878, 1165
Tru1I TTAA 6 cut(s) 375, 488, 558, 816, 1158, 1251
Tru9I TTAA 6 cut(s) 375, 488, 558, 816, 1158, 1251
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 4 cut(s) 608, 830, 953, 1469
TseI GCWGC 5 cut(s) 49, 219, 548, 1074, 1445
Tsp45I GTSAC 4 cut(s) 608, 830, 953, 1469
TspRI CASTG 1 cut(s) 175
Van91I CCANNNNNTGG 1 cut(s) 913
Vha464I CTTAAG 1 cut(s) 487
VspI ATTAAT 1 cut(s) 1158
XapI RAATTY 4 cut(s) 182, 355, 763, 1423
XmiI GTMKAC 4 cut(s) 370, 717, 783, 1284
XspI CTAG 1 cut(s) 1490
Zsp2I ATGCAT 4 cut(s) 481, 856, 1258, 1465
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.