FvH4_4g01551

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000144
Physical Location & Seq
Forward (+)
1379984 .. 1386946
6963 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g01551.t1

Sequence Viewer

Length: 1503 bp
ATGAATTCGACATGTGTAAACTATAAAGGAAAAGCGAAACCTCGACCGCCGCAAGATGTAAGATCAGAAGCAGCTCAGAGTCTCTTCCATCTACCCTCTCAGTCGCCTCTCTTTCTGATCAGCCTTGGATCTGTGCTTGGGGGTGTGGTAATCAAAGGACTCTTCCGTCGCCGCAAACCCGGCTGGGAGACACTCCCTGACGATATCATGGAGGTTATTGTTCCACACCTGAGTGTACGGGATCGCATACGCTTAAGCCTAGTTTGCAAGCCTTTGAGCTCAATTCCTATGCGCAAAGACATCTCTAGTTCACGACATACACTTCCCTGGTTAATACTCCCTCAACCTGAATCTAAAGCTCTGACTTGCAACGATACCTCCTTGAGCTTTTTCGACTTGCATCATGGCAAAGTTGTCAAGTTAGAGTTGCCTGAACCTGTTCACGGAGCGTGGTTTCGAGGGTGTTCCAAAGGTTGGTTGATTATGATCAAGGAAGATGAACACAACTCTTCAGTGTTTCTAGTAAATCCCATATCAGGAGTCCATCACCAACTTCCGTCTTTGAGAACGATTCCATCTTTCCAGGAGTATGTAGAAACAGCCACATGGAAAAAATATGGTGCCTTTGCTTTCTACAACCGCCTTGTGTTATCGACCTCCGATATCGATTCAGAAGAGTGTATTGTAGCAGCTACTCTTGGCCGTTTTACTGATAAAATACTGGTTCTTTGCAGACCTGGAGACAAAGAATGGAATGTCTTTCGGTTAACAGATGCAAATGGAACTTTGACTAATATATTATTTTCTTGTGGCATGCTATACGCCTTGGTTGTGAGTAAATATAAGATGGGCGTCGTTGCAACTCCTCAGATGTTGAACTTTGGAGATCAGTCGGTGGAGTTGAAGTTGGTCTATGACGTGAATCCAAACTACAATGATACGATGAAGTTGTTGAAGATCATGGTAACTATGAGACTAGATCTATTTTCAAGCATAGACAACATCAACGTTGAAAATCGTGGCAATAATCTGGAAGAGGAAGAGAATGGTGGTGGTGGCAATGATGTGGAAGAGGAAGAGAATGACGAAGGCTATGATGGAGGGGACAACTTCGAAGGAGAAGGTGATGATGAGACCGGAGAGTGGAAGCATGTTGACACTCCTTTCTTTCCCAGCCCATCTGAAGAGAAAGACATGCACCCGGACTTGAAGGAGATGCCCATGCACTTTGATTACAGGAGCAGAGAAGGCGCCGTATACTGCAATGGACAAGACGTCGAGATGATCATGACATCTTTTTCAAAACTAAAAGAAGTGAATTCGTTAGAGCTGAGTGGGAAGGAAAGGGATGCAGAGGAGGATGATTCTTCATGTGACCTTTGGTTGCACCTGCCTAGCAATAAGACCTTGAAGACATTTGTAGAGTTAGCCGATAAGGAACTTTTTCTAGATCTTGATATTTACTATGGATATTTTTATCCGGAGATTCATCCTTACATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

501

Amino Acids

56.72

Weight (kDa)

4.98

Isoelectric Point (pI)

47.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 130 - 293 4e-21 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1398
AasI GACNNNNNNGTC 1 cut(s) 165
AatII GACGTC 1 cut(s) 1278
Acc16I TGCGCA 1 cut(s) 293
Acc36I ACCTGC 1 cut(s) 1398
AccB1I GGYRCC 2 cut(s) 620, 1250
AccB7I CCANNNNNTGG 1 cut(s) 474
AccI GTMKAC 1 cut(s) 1257
AccIII TCCGGA 1 cut(s) 1480
AciI CCGC 4 cut(s) 47, 50, 172, 640
AclI AACGTT 1 cut(s) 1008
AclWI GGATC 2 cut(s) 136, 249
AcoI YGGCCR 1 cut(s) 700
AcsI RAATTY 2 cut(s) 4, 1318
AcuI CTGAAG 2 cut(s) 495, 1203
AcyI GRCGYC 3 cut(s) 852, 1251, 1275
AfaI GTAC 1 cut(s) 237
AfiI CCNNNNNNNGG 4 cut(s) 443, 474, 536, 1143
AflII CTTAAG 1 cut(s) 253
AflIII ACRYGT 2 cut(s) 11, 1497
AgsI TTSAA 8 cut(s) 877, 904, 955, 990, 1013, 1210, 1302, 1411
AhdI GACNNNNNGTC 1 cut(s) 1274
AjiI CACGTC 1 cut(s) 919
AjnI CCWGG 3 cut(s) 326, 582, 736
AleI CACNNNNGTG 1 cut(s) 231
AluBI AGCT 6 cut(s) 74, 279, 359, 387, 692, 1330
AluI AGCT 6 cut(s) 74, 279, 359, 387, 692, 1330
Alw21I GWGCWC 1 cut(s) 281
Alw26I GTCTC 5 cut(s) 86, 182, 735, 967, 1127
AlwI GGATC 2 cut(s) 136, 249
Aor13HI TCCGGA 1 cut(s) 1480
AoxI GGCC 1 cut(s) 700
ApeKI GCWGC 2 cut(s) 71, 689
ApoI RAATTY 2 cut(s) 4, 1318
Asp700I GAANNNNTTC 2 cut(s) 438, 1443
AspLEI GCGC 2 cut(s) 294, 1253
AsuC2I CCSGG 2 cut(s) 180, 1202
AsuHPI GGTGA 2 cut(s) 539, 1136
AsuII TTCGAA 1 cut(s) 1113
BanI GGYRCC 2 cut(s) 620, 1250
BanII GRGCYC 1 cut(s) 281
BbsI GAAGAC 1 cut(s) 1418
Bbv12I GWGCWC 1 cut(s) 281
BbvI GCAGC 2 cut(s) 83, 701
BccI CCATC 6 cut(s) 96, 552, 583, 841, 1091, 1186
BceAI ACGGC 2 cut(s) 687, 1238
BciT130I CCWGG 3 cut(s) 328, 584, 738
BclI TGATCA 3 cut(s) 117, 486, 1284
BcnI CCSGG 2 cut(s) 180, 1202
BcoDI GTCTC 5 cut(s) 86, 182, 735, 967, 1127
BfaI CTAG 6 cut(s) 260, 306, 521, 977, 1395, 1448
BfoI RGCGCY 1 cut(s) 1254
BfrI CTTAAG 1 cut(s) 253
BfuAI ACCTGC 1 cut(s) 1398
BglII AGATCT 2 cut(s) 979, 1450
BisI GCNGC 4 cut(s) 50, 72, 172, 690
BlsI GCNGC 4 cut(s) 51, 73, 173, 691
Bme1390I CCNGG 5 cut(s) 180, 328, 584, 738, 1202
BmeRI GACNNNNNGTC 1 cut(s) 1274
BmgBI CACGTC 1 cut(s) 919
BmiI GGNNCC 2 cut(s) 622, 1252
BmrFI CCNGG 5 cut(s) 180, 328, 584, 738, 1202
BmsI GCATC 4 cut(s) 409, 763, 1206, 1339
BpiI GAAGAC 1 cut(s) 1418
BpmI CTGGAG 1 cut(s) 759
Bpu14I TTCGAA 1 cut(s) 1113
BpuEI CTTGAG 1 cut(s) 403
BpuMI CCSGG 2 cut(s) 180, 1202
Bsa29I ATCGAT 1 cut(s) 666
BsaBI GATNNNNATC 1 cut(s) 485
BsaHI GRCGYC 3 cut(s) 852, 1251, 1275
BsaI GGTCTC 1 cut(s) 1127
BsaJI CCNNGG 3 cut(s) 124, 326, 825
BsaWI WCCGGW 2 cut(s) 1136, 1480
BsaXI ACNNNNNCTCC 4 cut(s) 362, 392, 890, 920
Bsc4I CCNNNNNNNGG 4 cut(s) 443, 474, 536, 1143
Bse1I ACTGG 1 cut(s) 726
Bse3DI GCAATG 2 cut(s) 1066, 1270
Bse8I GATNNNNATC 1 cut(s) 485
BseAI TCCGGA 1 cut(s) 1480
BseBI CCWGG 3 cut(s) 328, 584, 738
BseCI ATCGAT 1 cut(s) 666
BseDI CCNNGG 3 cut(s) 124, 326, 825
BseGI GGATG 3 cut(s) 1354, 1366, 1489
BseJI GATNNNNATC 1 cut(s) 485
BseLI CCNNNNNNNGG 4 cut(s) 443, 474, 536, 1143
BseMI GCAATG 2 cut(s) 1066, 1270
BseMII CTCAG 5 cut(s) 89, 113, 221, 881, 1322
BseNI ACTGG 1 cut(s) 726
BseRI GAGGAG 2 cut(s) 855, 1370
BseXI GCAGC 2 cut(s) 83, 701
BseYI CCCAGC 2 cut(s) 183, 1172
Bsh1285I CGRYCG 1 cut(s) 47
BshFI GGCC 1 cut(s) 702
BshNI GGYRCC 2 cut(s) 620, 1250
BshVI ATCGAT 1 cut(s) 666
BsiEI CGRYCG 1 cut(s) 47
BsiHKAI GWGCWC 1 cut(s) 281
BsiSI CCGG 4 cut(s) 180, 1137, 1202, 1481
BslFI GGGAC 1 cut(s) 1118
BslI CCNNNNNNNGG 4 cut(s) 443, 474, 536, 1143
BsmAI GTCTC 5 cut(s) 86, 182, 735, 967, 1127
BsmFI GGGAC 1 cut(s) 1118
BsnI GGCC 1 cut(s) 702
Bso31I GGTCTC 1 cut(s) 1127
Bsp119I TTCGAA 1 cut(s) 1113
Bsp1286I GDGCHC 1 cut(s) 281
Bsp13I TCCGGA 1 cut(s) 1480
BspACI CCGC 4 cut(s) 47, 50, 172, 640
BspANI GGCC 1 cut(s) 702
BspCNI CTCAG 5 cut(s) 88, 112, 222, 880, 1323
BspDI ATCGAT 1 cut(s) 666
BspEI TCCGGA 1 cut(s) 1480
BspHI TCATGA 1 cut(s) 1287
BspLI GGNNCC 2 cut(s) 622, 1252
BspMI ACCTGC 1 cut(s) 1398
BspPI GGATC 2 cut(s) 136, 249
BspT104I TTCGAA 1 cut(s) 1113
BspT107I GGYRCC 2 cut(s) 620, 1250
BspTI CTTAAG 1 cut(s) 253
BspTNI GGTCTC 1 cut(s) 1127
BsrDI GCAATG 2 cut(s) 1066, 1270
BsrI ACTGG 1 cut(s) 726
BssECI CCNNGG 3 cut(s) 124, 326, 825
BssNAI GTATAC 1 cut(s) 1258
BssNI GRCGYC 3 cut(s) 852, 1251, 1275
BssT1I CCWWGG 2 cut(s) 124, 825
Bst1107I GTATAC 1 cut(s) 1258
Bst2UI CCWGG 3 cut(s) 328, 584, 738
Bst6I CTCTTC 9 cut(s) 89, 167, 514, 669, 1029, 1035, 1065, 1071, 1179
BstACI GRCGYC 3 cut(s) 852, 1251, 1275
BstAFI CTTAAG 1 cut(s) 253
BstBI TTCGAA 1 cut(s) 1113
BstC8I GCNNGC 2 cut(s) 269, 815
BstDEI CTNAG 5 cut(s) 75, 99, 230, 867, 1331
BstF5I GGATG 3 cut(s) 1354, 1366, 1489
BstH2I RGCGCY 1 cut(s) 1254
BstHHI GCGC 2 cut(s) 294, 1253
BstMAI GTCTC 5 cut(s) 86, 182, 735, 967, 1127
BstMCI CGRYCG 1 cut(s) 47
BstMWI GCNNNNNNNGC 3 cut(s) 180, 264, 1248
BstNI CCWGG 3 cut(s) 328, 584, 738
BstNSI RCATGY 5 cut(s) 15, 817, 1154, 1198, 1501
BstSCI CCNGG 5 cut(s) 178, 326, 582, 736, 1200
BstV1I GCAGC 2 cut(s) 83, 701
BstV2I GAAGAC 1 cut(s) 1418
BstX2I RGATCY 3 cut(s) 128, 979, 1450
BstYI RGATCY 3 cut(s) 128, 979, 1450
BstZ17I GTATAC 1 cut(s) 1258
Bsu15I ATCGAT 1 cut(s) 666
BsuRI GGCC 1 cut(s) 702
BsuTUI ATCGAT 1 cut(s) 666
BtrI CACGTC 1 cut(s) 919
BtsCI GGATG 3 cut(s) 1354, 1366, 1489
BtsIMutI CAGTG 1 cut(s) 519
BveI ACCTGC 1 cut(s) 1398
Cac8I GCNNGC 2 cut(s) 269, 815
CciI TCATGA 1 cut(s) 1287
CfoI GCGC 2 cut(s) 294, 1253
ClaI ATCGAT 1 cut(s) 666
CseI GACGC 1 cut(s) 841
Csp6I GTAC 1 cut(s) 236
CviQI GTAC 1 cut(s) 236
DdeI CTNAG 5 cut(s) 75, 99, 230, 867, 1331
DinI GGCGCC 1 cut(s) 1252
DrdI GACNNNNNNGTC 1 cut(s) 165
DriI GACNNNNNGTC 1 cut(s) 1274
DseDI GACNNNNNNGTC 1 cut(s) 165
EaeI YGGCCR 1 cut(s) 700
Eam1104I CTCTTC 9 cut(s) 89, 167, 514, 669, 1029, 1035, 1065, 1071, 1179
Eam1105I GACNNNNNGTC 1 cut(s) 1274
EarI CTCTTC 9 cut(s) 89, 167, 514, 669, 1029, 1035, 1065, 1071, 1179
Ecl136II GAGCTC 1 cut(s) 279
Eco130I CCWWGG 2 cut(s) 124, 825
Eco24I GRGCYC 1 cut(s) 281
Eco31I GGTCTC 1 cut(s) 1127
Eco32I GATATC 2 cut(s) 205, 664
Eco53kI GAGCTC 1 cut(s) 279
Eco57I CTGAAG 2 cut(s) 495, 1203
EcoICRI GAGCTC 1 cut(s) 279
EcoRI GAATTC 2 cut(s) 4, 1318
EcoRII CCWGG 3 cut(s) 326, 582, 736
EcoRV GATATC 2 cut(s) 205, 664
EcoT14I CCWWGG 2 cut(s) 124, 825
EcoT38I GRGCYC 1 cut(s) 281
EgeI GGCGCC 1 cut(s) 1252
EheI GGCGCC 1 cut(s) 1252
ErhI CCWWGG 2 cut(s) 124, 825
FaqI GGGAC 1 cut(s) 1118
FbaI TGATCA 3 cut(s) 117, 486, 1284
FblI GTMKAC 1 cut(s) 1257
Fnu4HI GCNGC 4 cut(s) 50, 72, 172, 690
FokI GGATG 3 cut(s) 1361, 1373, 1476
FriOI GRGCYC 1 cut(s) 281
Fsp4HI GCNGC 4 cut(s) 50, 72, 172, 690
FspBI CTAG 6 cut(s) 260, 306, 521, 977, 1395, 1448
FspI TGCGCA 1 cut(s) 293
GlaI GCGC 2 cut(s) 293, 1252
GluI GCNGC 4 cut(s) 50, 72, 172, 690
GsaI CCCAGC 2 cut(s) 187, 1176
GsuI CTGGAG 1 cut(s) 759
HaeII RGCGCY 1 cut(s) 1254
HaeIII GGCC 1 cut(s) 702
HapII CCGG 4 cut(s) 180, 1137, 1202, 1481
HgaI GACGC 1 cut(s) 841
HhaI GCGC 2 cut(s) 294, 1253
Hin1I GRCGYC 3 cut(s) 852, 1251, 1275
Hin6I GCGC 2 cut(s) 292, 1251
HinP1I GCGC 2 cut(s) 292, 1251
HincII GTYRAC 2 cut(s) 768, 1156
HindII GTYRAC 2 cut(s) 768, 1156
HinfI GANTC 9 cut(s) 79, 159, 350, 540, 571, 668, 922, 1364, 1486
HpaI GTTAAC 1 cut(s) 768
HpaII CCGG 4 cut(s) 180, 1137, 1202, 1481
HphI GGTGA 2 cut(s) 539, 1136
Hpy166II GTNNAC 7 cut(s) 19, 236, 311, 442, 768, 1156, 1258
Hpy188I TCNGA 8 cut(s) 67, 78, 117, 363, 661, 673, 870, 1183
Hpy188III TCNNGA 8 cut(s) 312, 537, 1031, 1279, 1288, 1448, 1454, 1481
Hpy8I GTNNAC 7 cut(s) 19, 236, 311, 442, 768, 1156, 1258
Hpy99I CGWCG 3 cut(s) 171, 857, 1280
HpyAV CCTTC 6 cut(s) 1082, 1109, 1115, 1204, 1241, 1333
HpyCH4IV ACGT 3 cut(s) 918, 1008, 1275
HpyF10VI GCNNNNNNNGC 3 cut(s) 180, 264, 1248
HpyF3I CTNAG 5 cut(s) 75, 99, 230, 867, 1331
HpySE526I ACGT 3 cut(s) 918, 1008, 1275
Hsp92I GRCGYC 3 cut(s) 852, 1251, 1275
HspAI GCGC 2 cut(s) 292, 1251
KasI GGCGCC 1 cut(s) 1250
Kpn2I TCCGGA 1 cut(s) 1480
Ksp22I TGATCA 3 cut(s) 117, 486, 1284
KspAI GTTAAC 1 cut(s) 768
LmnI GCTCC 2 cut(s) 446, 1239
Lsp1109I GCAGC 2 cut(s) 83, 701
LweI GCATC 4 cut(s) 409, 763, 1206, 1339
MaeI CTAG 6 cut(s) 260, 306, 521, 977, 1395, 1448
MaeII ACGT 3 cut(s) 918, 1008, 1275
MaeIII GTNAC 2 cut(s) 964, 1373
MflI RGATCY 3 cut(s) 128, 979, 1450
MhlI GDGCHC 1 cut(s) 281
MluCI AATT 3 cut(s) 4, 282, 1318
Mly113I GGCGCC 1 cut(s) 1251
MlyI GAGTC 3 cut(s) 88, 153, 549
MroI TCCGGA 1 cut(s) 1480
MroXI GAANNNNTTC 2 cut(s) 438, 1443
MseI TTAA 3 cut(s) 254, 332, 767
MslI CAYNNNNRTG 1 cut(s) 231
MspCI CTTAAG 1 cut(s) 253
MspI CCGG 4 cut(s) 180, 1137, 1202, 1481
MspR9I CCNGG 5 cut(s) 180, 328, 584, 738, 1202
MvaI CCWGG 3 cut(s) 328, 584, 738
MwoI GCNNNNNNNGC 3 cut(s) 180, 264, 1248
NarI GGCGCC 1 cut(s) 1251
NciI CCSGG 2 cut(s) 180, 1202
NlaIV GGNNCC 2 cut(s) 622, 1252
NmuCI GTSAC 1 cut(s) 1373
NsbI TGCGCA 1 cut(s) 293
NspI RCATGY 5 cut(s) 15, 817, 1154, 1198, 1501
NspV TTCGAA 1 cut(s) 1113
OliI CACNNNNGTG 1 cut(s) 231
PaeI GCATGC 1 cut(s) 817
PagI TCATGA 1 cut(s) 1287
PaqCI CACCTGC 1 cut(s) 1398
PciI ACATGT 2 cut(s) 11, 1497
PdmI GAANNNNTTC 2 cut(s) 438, 1443
PfeI GAWTC 6 cut(s) 350, 571, 668, 922, 1364, 1486
PflMI CCANNNNNTGG 1 cut(s) 474
PfoI TCCNGGA 1 cut(s) 582
PkrI GCNGC 4 cut(s) 51, 73, 173, 691
PleI GAGTC 3 cut(s) 87, 153, 548
PluTI GGCGCC 1 cut(s) 1254
PpsI GAGTC 3 cut(s) 87, 153, 548
PscI ACATGT 2 cut(s) 11, 1497
Psp124BI GAGCTC 1 cut(s) 281
Psp1406I AACGTT 1 cut(s) 1008
Psp6I CCWGG 3 cut(s) 326, 582, 736
PspFI CCCAGC 2 cut(s) 183, 1172
PspGI CCWGG 3 cut(s) 326, 582, 736
PspN4I GGNNCC 2 cut(s) 622, 1252
PsuI RGATCY 3 cut(s) 128, 979, 1450
RsaI GTAC 1 cut(s) 237
RsaNI GTAC 1 cut(s) 236
RseI CAYNNNNRTG 1 cut(s) 231
SacI GAGCTC 1 cut(s) 281
SaqAI TTAA 3 cut(s) 254, 332, 767
SatI GCNGC 4 cut(s) 50, 72, 172, 690
SchI GAGTC 3 cut(s) 88, 153, 549
ScrFI CCNGG 5 cut(s) 180, 328, 584, 738, 1202
SduI GDGCHC 1 cut(s) 281
SfaNI GCATC 4 cut(s) 409, 763, 1206, 1339
SfoI GGCGCC 1 cut(s) 1252
SfuI TTCGAA 1 cut(s) 1113
SmiMI CAYNNNNRTG 1 cut(s) 231
SmlI CTYRAG 2 cut(s) 253, 382
SmoI CTYRAG 2 cut(s) 253, 382
SphI GCATGC 1 cut(s) 817
Sse9I AATT 3 cut(s) 4, 282, 1318
SsiI CCGC 4 cut(s) 47, 50, 172, 640
SspDI GGCGCC 1 cut(s) 1250
SspMI CTAG 6 cut(s) 260, 306, 521, 977, 1395, 1448
SstI GAGCTC 1 cut(s) 281
StyD4I CCNGG 5 cut(s) 178, 326, 582, 736, 1200
StyI CCWWGG 2 cut(s) 124, 825
TaiI ACGT 3 cut(s) 921, 1011, 1278
TaqI TCGA 8 cut(s) 8, 43, 393, 457, 653, 666, 1113, 1278
TasI AATT 3 cut(s) 4, 282, 1318
TauI GCSGC 2 cut(s) 52, 174
TfiI GAWTC 6 cut(s) 350, 571, 668, 922, 1364, 1486
Tru1I TTAA 3 cut(s) 254, 332, 767
Tru9I TTAA 3 cut(s) 254, 332, 767
TscAI CASTG 1 cut(s) 519
TseFI GTSAC 1 cut(s) 1373
TseI GCWGC 2 cut(s) 71, 689
Tsp45I GTSAC 1 cut(s) 1373
TspDTI ATGAA 5 cut(s) 17, 513, 959, 1359, 1478
TspGWI ACGGA 3 cut(s) 155, 459, 546
TspRI CASTG 1 cut(s) 519
Van91I CCANNNNNTGG 1 cut(s) 474
Vha464I CTTAAG 1 cut(s) 253
XapI RAATTY 2 cut(s) 4, 1318
XbaI TCTAGA 1 cut(s) 1447
XceI RCATGY 5 cut(s) 15, 817, 1154, 1198, 1501
XmiI GTMKAC 1 cut(s) 1257
XmnI GAANNNNTTC 2 cut(s) 438, 1443
XspI CTAG 6 cut(s) 260, 306, 521, 977, 1395, 1448
ZraI GACGTC 1 cut(s) 1276
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.