pycom03g01090

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000144
Physical Location & Seq
Reverse (-)
877689 .. 879157
1469 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g01090.1

Sequence Viewer

Length: 1368 bp
ATGCTGGCGCCGAAGGAGACGAACGAGGACTTGGCTCTCCCCGACGACGCGTGCCCCGTTCTCAACGTCGGCAAGGAGAAGGAGATCGGCATGCAGGACTTGGAGAAGAAGAATAACGGCGTCAACGAGGTTGAAGTTGTAGATGGGTGTTGGCAGAGACCACCTTTGGATATCATGGAGAATATCGTACAACGCCTCGACTTACTTGATCGGATTCGCTTAAGCATCCTCTGCAAGTACTGGAGAATGATCGTTATGCAAATTATCCGCAGTGCTCCGCCATTCCCATGGTTAGTAGGCCCATCTGAAGCAGCTTCCTGGTTATTATTTGGAACTCAACCGAGTTCCAGTTACTTAGAGTTCTCCATCCCACCTGAAGGTAAAGTTATCAAGCTAGGGTTGCCTAAGCGAAATCAAGGGCGCTTTCATAGTTCCTCCAAAGGTTGGTTGATAATGGTCAATGAAGAACACTCTGAAGTATTTCTACTAAACCCTTTTTCAGGAGCCAAACACGAACTTCCTTCCTTGAGAAGAATCCCATATTTCAAGAAATTTGGTGGAAAACCCGACCGATATGTTGACAAAATTGCCCTATCGAGTTCAAATCTATCAGAATGTACGGTGGCAGCAATTTTTTCTTCTGGGGAAATTGGTGTGTGCAGGCCAAAGAAAGGTTGGAGAATATCTCGGATGTGGGATAGAAAAACGTATCCTTCATGCCTACTGTTTTCGTCTTGTGGCATGCTATATGTCTTAGTCGAAAACAGTATGTCTGGACACGTTGTAACTCGCACATTACGCCTTGGAGATGGTGATGAGCTGGAATTGAAGTTGGTTTATGACAAAGAAAAATCACGAAAGGGTTCATTCAATTGCTGGGGTGAAGGTGACTTTTTGGATGTCAAGGTCAAAGACGAGTCATATTTGATAGAATCAACAACCTGCAACGAAGTCTTGTTGATCCGTCAAATTAGGGATCAAATGAAAACCGATTTGAATACCTGGACAAGGACTCATGATTTTCTAGTTTACAAGATCGATCCGGAGAATGGTGAAAGCTTTATCGAGGTACAGAGTTTGGGAAATCAAATATTATTTGTAACAAGGCGAGGCTGTTCCTTTTCCTTTCCGGTGAGTAATTCTAACGGGTTGGAAGTGAACTGCATTTATTTTGTGGAGTTAGGAGGAATTGACCACGACGACATGTTTTGTGTTGAGAATTCTGGACATAGTAAAACCACCATGTGTGATTTTTCTCATCCTTTCTACTTAGATGGCCGGAAATTTGGAAGATCCAAAAAATTTTATGATAGCATTGAAACCGAATTCACTTGGGGAGTGAGTTGGTACAGTCCGAGTTTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

456

Amino Acids

51.91

Weight (kDa)

6.0

Isoelectric Point (pI)

43.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 55 - 85 8.2e-07 F-box domain
Beta-prop_KIB1-4 PF03478 123 - 402 5e-37 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 950
AccB1I GGYRCC 1 cut(s) 7
AccB7I CCANNNNNTGG 1 cut(s) 444
AccII CGCG 1 cut(s) 50
AccIII TCCGGA 1 cut(s) 1042
AciI CCGC 2 cut(s) 268, 278
AclWI GGATC 4 cut(s) 955, 984, 1034, 1287
AcoI YGGCCR 1 cut(s) 1276
AcsI RAATTY 5 cut(s) 551, 1219, 1283, 1301, 1325
AcuI CTGAAG 3 cut(s) 327, 396, 495
AcyI GRCGYC 2 cut(s) 8, 120
AdeI CACNNNGTG 1 cut(s) 1245
AfaI GTAC 5 cut(s) 189, 239, 619, 1071, 1349
AfiI CCNNNNNNNGG 6 cut(s) 377, 444, 500, 671, 1008, 1049
AflII CTTAAG 1 cut(s) 220
AflIII ACRYGT 3 cut(s) 48, 778, 1203
AgsI TTSAA 7 cut(s) 134, 547, 603, 829, 871, 997, 1319
AjnI CCWGG 2 cut(s) 317, 1001
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 4 cut(s) 314, 394, 820, 1059
AluI AGCT 4 cut(s) 314, 394, 820, 1059
Alw21I GWGCWC 1 cut(s) 277
Alw26I GTCTC 2 cut(s) 11, 151
AlwI GGATC 4 cut(s) 955, 984, 1034, 1287
Aor13HI TCCGGA 1 cut(s) 1042
AoxI GGCC 3 cut(s) 298, 662, 1276
ApeKI GCWGC 2 cut(s) 311, 626
ApoI RAATTY 5 cut(s) 551, 1219, 1283, 1301, 1325
Asp700I GAANNNNTTC 2 cut(s) 480, 862
AspLEI GCGC 2 cut(s) 10, 423
AspS9I GGNCC 1 cut(s) 299
AsuHPI GGTGA 5 cut(s) 824, 893, 899, 1064, 1144
BaeGI GKGCMC 1 cut(s) 56
BanI GGYRCC 1 cut(s) 7
BarI GAAGNNNNNNTAC 2 cut(s) 468, 500
Bbv12I GWGCWC 1 cut(s) 277
BbvI GCAGC 2 cut(s) 323, 638
BccI CCATC 5 cut(s) 137, 310, 374, 803, 1268
BceAI ACGGC 1 cut(s) 133
BciT130I CCWGG 2 cut(s) 319, 1003
BciVI GTATCC 1 cut(s) 720
BcoDI GTCTC 2 cut(s) 11, 151
BfaI CTAG 2 cut(s) 395, 1025
BfoI RGCGCY 2 cut(s) 11, 424
BfrI CTTAAG 1 cut(s) 220
BfuAI ACCTGC 1 cut(s) 950
BfuI GTATCC 1 cut(s) 720
BisI GCNGC 2 cut(s) 312, 627
BlsI GCNGC 2 cut(s) 313, 628
BmcAI AGTACT 1 cut(s) 239
Bme1390I CCNGG 2 cut(s) 319, 1003
BmgT120I GGNCC 1 cut(s) 299
BmiI GGNNCC 2 cut(s) 9, 505
BmrFI CCNGG 2 cut(s) 319, 1003
BmsI GCATC 1 cut(s) 234
BplI GAGNNNNNCTC 2 cut(s) 670, 702
BpmI CTGGAG 1 cut(s) 262
Bpu10I CCTNAGC 1 cut(s) 405
BpuEI CTTGAG 1 cut(s) 547
Bsa29I ATCGAT 1 cut(s) 1038
BsaHI GRCGYC 2 cut(s) 8, 120
BsaI GGTCTC 1 cut(s) 151
BsaJI CCNNGG 2 cut(s) 287, 802
BsaWI WCCGGW 2 cut(s) 1042, 1129
Bsc4I CCNNNNNNNGG 6 cut(s) 377, 444, 500, 671, 1008, 1049
Bse1I ACTGG 2 cut(s) 245, 348
BseAI TCCGGA 1 cut(s) 1042
BseBI CCWGG 2 cut(s) 319, 1003
BseCI ATCGAT 1 cut(s) 1038
BseDI CCNNGG 2 cut(s) 287, 802
BseGI GGATG 5 cut(s) 225, 366, 696, 904, 1258
BseLI CCNNNNNNNGG 6 cut(s) 377, 444, 500, 671, 1008, 1049
BseNI ACTGG 2 cut(s) 245, 348
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 2 cut(s) 323, 638
BseYI CCCAGC 1 cut(s) 876
BsgI GTGCAG 1 cut(s) 679
Bsh1236I CGCG 1 cut(s) 50
Bsh1285I CGRYCG 1 cut(s) 571
BshFI GGCC 3 cut(s) 300, 664, 1278
BshNI GGYRCC 1 cut(s) 7
BshVI ATCGAT 1 cut(s) 1038
BsiEI CGRYCG 1 cut(s) 571
BsiHKAI GWGCWC 1 cut(s) 277
BsiSI CCGG 3 cut(s) 1043, 1130, 1279
BslI CCNNNNNNNGG 6 cut(s) 377, 444, 500, 671, 1008, 1049
BsmAI GTCTC 2 cut(s) 11, 151
BsmBI CGTCTC 1 cut(s) 11
BsnI GGCC 3 cut(s) 300, 664, 1278
Bso31I GGTCTC 1 cut(s) 151
Bsp1286I GDGCHC 2 cut(s) 56, 277
Bsp13I TCCGGA 1 cut(s) 1042
Bsp143I GATC 8 cut(s) 84, 208, 249, 960, 976, 1035, 1039, 1292
Bsp19I CCATGG 1 cut(s) 287
BspACI CCGC 2 cut(s) 268, 278
BspANI GGCC 3 cut(s) 300, 664, 1278
BspDI ATCGAT 1 cut(s) 1038
BspEI TCCGGA 1 cut(s) 1042
BspFNI CGCG 1 cut(s) 50
BspHI TCATGA 1 cut(s) 1015
BspLI GGNNCC 2 cut(s) 9, 505
BspMI ACCTGC 1 cut(s) 950
BspPI GGATC 4 cut(s) 955, 984, 1034, 1287
BspT107I GGYRCC 1 cut(s) 7
BspTI CTTAAG 1 cut(s) 220
BspTNI GGTCTC 1 cut(s) 151
BsrI ACTGG 2 cut(s) 245, 348
BssECI CCNNGG 2 cut(s) 287, 802
BssMI GATC 8 cut(s) 84, 208, 249, 960, 976, 1035, 1039, 1292
BssNI GRCGYC 2 cut(s) 8, 120
BssT1I CCWWGG 2 cut(s) 287, 802
Bst2UI CCWGG 2 cut(s) 319, 1003
Bst4CI ACNGT 5 cut(s) 622, 726, 767, 1352, 1365
BstACI GRCGYC 2 cut(s) 8, 120
BstAFI CTTAAG 1 cut(s) 220
BstAPI GCANNNNNTGC 1 cut(s) 231
BstC8I GCNNGC 5 cut(s) 6, 52, 92, 662, 743
BstDEI CTNAG 4 cut(s) 355, 405, 754, 1270
BstDSI CCRYGG 1 cut(s) 287
BstENI CCTNNNNNAGG 2 cut(s) 498, 1006
BstF5I GGATG 5 cut(s) 225, 366, 696, 904, 1258
BstFNI CGCG 1 cut(s) 50
BstH2I RGCGCY 2 cut(s) 11, 424
BstHHI GCGC 2 cut(s) 10, 423
BstKTI GATC 8 cut(s) 87, 211, 252, 963, 979, 1038, 1042, 1295
BstMAI GTCTC 2 cut(s) 11, 151
BstMBI GATC 8 cut(s) 84, 208, 249, 960, 976, 1035, 1039, 1292
BstMCI CGRYCG 1 cut(s) 571
BstMWI GCNNNNNNNGC 3 cut(s) 231, 400, 798
BstNI CCWGG 2 cut(s) 319, 1003
BstNSI RCATGY 3 cut(s) 94, 745, 1207
BstSCI CCNGG 2 cut(s) 317, 1001
BstSLI GKGCMC 1 cut(s) 56
BstUI CGCG 1 cut(s) 50
BstV1I GCAGC 2 cut(s) 323, 638
BstX2I RGATCY 1 cut(s) 1292
BstXI CCANNNNNNTGG 1 cut(s) 288
BstYI RGATCY 1 cut(s) 1292
Bsu15I ATCGAT 1 cut(s) 1038
BsuI GTATCC 1 cut(s) 720
BsuRI GGCC 3 cut(s) 300, 664, 1278
BsuTUI ATCGAT 1 cut(s) 1038
BtgI CCRYGG 1 cut(s) 287
BtsCI GGATG 5 cut(s) 225, 366, 696, 904, 1258
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 1 cut(s) 277
BveI ACCTGC 1 cut(s) 950
Cac8I GCNNGC 5 cut(s) 6, 52, 92, 662, 743
CciI TCATGA 1 cut(s) 1015
CfoI GCGC 2 cut(s) 10, 423
Cfr13I GGNCC 1 cut(s) 299
ClaI ATCGAT 1 cut(s) 1038
CseI GACGC 2 cut(s) 56, 109
Csp6I GTAC 5 cut(s) 188, 238, 618, 1070, 1348
CviAII CATG 8 cut(s) 91, 175, 288, 717, 742, 1016, 1204, 1243
CviQI GTAC 5 cut(s) 188, 238, 618, 1070, 1348
DdeI CTNAG 4 cut(s) 355, 405, 754, 1270
DinI GGCGCC 1 cut(s) 9
DpnI GATC 8 cut(s) 86, 210, 251, 962, 978, 1037, 1041, 1294
DpnII GATC 8 cut(s) 84, 208, 249, 960, 976, 1035, 1039, 1292
DraIII CACNNNGTG 1 cut(s) 1245
EaeI YGGCCR 1 cut(s) 1276
EciI GGCGGA 1 cut(s) 267
Eco130I CCWWGG 2 cut(s) 287, 802
Eco31I GGTCTC 1 cut(s) 151
Eco32I GATATC 1 cut(s) 172
Eco57I CTGAAG 3 cut(s) 327, 396, 495
EcoNI CCTNNNNNAGG 2 cut(s) 498, 1006
EcoRI GAATTC 2 cut(s) 1219, 1325
EcoRII CCWGG 2 cut(s) 317, 1001
EcoRV GATATC 1 cut(s) 172
EcoT14I CCWWGG 2 cut(s) 287, 802
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 2 cut(s) 287, 802
Esp3I CGTCTC 1 cut(s) 11
FaeI CATG 8 cut(s) 94, 178, 291, 720, 745, 1019, 1207, 1246
FatI CATG 8 cut(s) 90, 174, 287, 716, 741, 1015, 1203, 1242
Fnu4HI GCNGC 2 cut(s) 312, 627
FokI GGATG 5 cut(s) 212, 353, 703, 911, 1245
Fsp4HI GCNGC 2 cut(s) 312, 627
FspBI CTAG 2 cut(s) 395, 1025
GlaI GCGC 2 cut(s) 9, 422
GluI GCNGC 2 cut(s) 312, 627
GsaI CCCAGC 1 cut(s) 880
GsuI CTGGAG 1 cut(s) 262
HaeII RGCGCY 2 cut(s) 11, 424
HaeIII GGCC 3 cut(s) 300, 664, 1278
HapII CCGG 3 cut(s) 1043, 1130, 1279
HgaI GACGC 2 cut(s) 56, 109
HhaI GCGC 2 cut(s) 10, 423
Hin1I GRCGYC 2 cut(s) 8, 120
Hin1II CATG 8 cut(s) 94, 178, 291, 720, 745, 1019, 1207, 1246
Hin6I GCGC 2 cut(s) 8, 421
HinP1I GCGC 2 cut(s) 8, 421
HincII GTYRAC 2 cut(s) 124, 580
HindII GTYRAC 2 cut(s) 124, 580
HindIII AAGCTT 1 cut(s) 1057
HinfI GANTC 5 cut(s) 214, 534, 917, 932, 1012
HpaII CCGG 3 cut(s) 1043, 1130, 1279
HphI GGTGA 5 cut(s) 824, 893, 899, 1064, 1144
Hpy166II GTNNAC 5 cut(s) 124, 580, 1030, 1159, 1361
Hpy188I TCNGA 6 cut(s) 213, 307, 475, 613, 690, 1356
Hpy188III TCNNGA 7 cut(s) 501, 547, 774, 855, 1016, 1043, 1224
Hpy8I GTNNAC 5 cut(s) 124, 580, 1030, 1159, 1361
Hpy99I CGWCG 4 cut(s) 47, 50, 71, 1202
HpyAV CCTTC 6 cut(s) 7, 73, 371, 531, 723, 878
HpyCH4III ACNGT 5 cut(s) 622, 726, 767, 1352, 1365
HpyCH4IV ACGT 3 cut(s) 66, 707, 780
HpyCH4V TGCA 6 cut(s) 94, 234, 259, 660, 945, 1164
HpyF10VI GCNNNNNNNGC 3 cut(s) 231, 400, 798
HpyF3I CTNAG 4 cut(s) 355, 405, 754, 1270
HpySE526I ACGT 3 cut(s) 66, 707, 780
Hsp92I GRCGYC 2 cut(s) 8, 120
Hsp92II CATG 8 cut(s) 94, 178, 291, 720, 745, 1019, 1207, 1246
HspAI GCGC 2 cut(s) 8, 421
KasI GGCGCC 1 cut(s) 7
Kpn2I TCCGGA 1 cut(s) 1042
Kzo9I GATC 8 cut(s) 84, 208, 249, 960, 976, 1035, 1039, 1292
LmnI GCTCC 2 cut(s) 280, 503
Lsp1109I GCAGC 2 cut(s) 323, 638
LweI GCATC 1 cut(s) 234
MaeI CTAG 2 cut(s) 395, 1025
MaeII ACGT 3 cut(s) 66, 707, 780
MaeIII GTNAC 4 cut(s) 350, 784, 887, 1099
MalI GATC 8 cut(s) 86, 210, 251, 962, 978, 1037, 1041, 1294
MboI GATC 8 cut(s) 84, 208, 249, 960, 976, 1035, 1039, 1292
MboII GAAGA 6 cut(s) 118, 121, 476, 543, 630, 1302
MfeI CAATTG 1 cut(s) 871
MflI RGATCY 1 cut(s) 1292
MhlI GDGCHC 2 cut(s) 56, 277
MluI ACGCGT 1 cut(s) 48
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 2 cut(s) 926, 1006
MmeI TCCRAC 2 cut(s) 656, 1131
MnlI CCTC 8 cut(s) 19, 121, 206, 239, 445, 1060, 1103, 1178
MroI TCCGGA 1 cut(s) 1042
MroXI GAANNNNTTC 2 cut(s) 480, 862
MseI TTAA 1 cut(s) 221
MslI CAYNNNNRTG 1 cut(s) 286
MspCI CTTAAG 1 cut(s) 220
MspI CCGG 3 cut(s) 1043, 1130, 1279
MspR9I CCNGG 2 cut(s) 319, 1003
MunI CAATTG 1 cut(s) 871
MvaI CCWGG 2 cut(s) 319, 1003
MvnI CGCG 1 cut(s) 50
MwoI GCNNNNNNNGC 3 cut(s) 231, 400, 798
NarI GGCGCC 1 cut(s) 8
NcoI CCATGG 1 cut(s) 287
NdeII GATC 8 cut(s) 84, 208, 249, 960, 976, 1035, 1039, 1292
NlaIII CATG 8 cut(s) 94, 178, 291, 720, 745, 1019, 1207, 1246
NlaIV GGNNCC 2 cut(s) 9, 505
NmuCI GTSAC 1 cut(s) 887
NspI RCATGY 3 cut(s) 94, 745, 1207
PaeI GCATGC 2 cut(s) 94, 745
PagI TCATGA 1 cut(s) 1015
PciI ACATGT 1 cut(s) 1203
PcsI WCGNNNNNNNCGW 2 cut(s) 54, 123
PdmI GAANNNNTTC 2 cut(s) 480, 862
PfeI GAWTC 3 cut(s) 214, 534, 932
PflMI CCANNNNNTGG 1 cut(s) 444
PkrI GCNGC 2 cut(s) 313, 628
PleI GAGTC 2 cut(s) 925, 1006
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 2 cut(s) 925, 1006
PscI ACATGT 1 cut(s) 1203
Psp6I CCWGG 2 cut(s) 317, 1001
PspFI CCCAGC 1 cut(s) 876
PspGI CCWGG 2 cut(s) 317, 1001
PspN4I GGNNCC 2 cut(s) 9, 505
PspPI GGNCC 1 cut(s) 299
PsuI RGATCY 1 cut(s) 1292
RsaI GTAC 5 cut(s) 189, 239, 619, 1071, 1349
RsaNI GTAC 5 cut(s) 188, 238, 618, 1070, 1348
RseI CAYNNNNRTG 1 cut(s) 286
SaqAI TTAA 1 cut(s) 221
SatI GCNGC 2 cut(s) 312, 627
Sau3AI GATC 8 cut(s) 84, 208, 249, 960, 976, 1035, 1039, 1292
Sau96I GGNCC 1 cut(s) 299
ScaI AGTACT 1 cut(s) 239
SchI GAGTC 2 cut(s) 926, 1006
ScrFI CCNGG 2 cut(s) 319, 1003
SduI GDGCHC 2 cut(s) 56, 277
SfaNI GCATC 1 cut(s) 234
SfoI GGCGCC 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 286
SmlI CTYRAG 2 cut(s) 220, 526
SmoI CTYRAG 2 cut(s) 220, 526
SphI GCATGC 2 cut(s) 94, 745
SsiI CCGC 2 cut(s) 268, 278
SspDI GGCGCC 1 cut(s) 7
SspI AATATT 1 cut(s) 1092
SspMI CTAG 2 cut(s) 395, 1025
StyD4I CCNGG 2 cut(s) 317, 1001
StyI CCWWGG 2 cut(s) 287, 802
TaaI ACNGT 5 cut(s) 622, 726, 767, 1352, 1365
TaiI ACGT 3 cut(s) 69, 710, 783
TaqI TCGA 5 cut(s) 198, 596, 759, 1038, 1065
TaqII GACCGA 1 cut(s) 585
TatI WGTACW 1 cut(s) 237
TfiI GAWTC 3 cut(s) 214, 534, 932
Tru1I TTAA 1 cut(s) 221
Tru9I TTAA 1 cut(s) 221
TscAI CASTG 1 cut(s) 277
TseFI GTSAC 1 cut(s) 887
TseI GCWGC 2 cut(s) 311, 626
Tsp45I GTSAC 1 cut(s) 887
TspDTI ATGAA 5 cut(s) 416, 477, 705, 855, 998
TspGWI ACGGA 1 cut(s) 953
TspRI CASTG 1 cut(s) 277
Van91I CCANNNNNTGG 1 cut(s) 444
Vha464I CTTAAG 1 cut(s) 220
XagI CCTNNNNNAGG 2 cut(s) 498, 1006
XapI RAATTY 5 cut(s) 551, 1219, 1283, 1301, 1325
XceI RCATGY 3 cut(s) 94, 745, 1207
XcmI CCANNNNNNNNNTGG 1 cut(s) 672
XmnI GAANNNNTTC 2 cut(s) 480, 862
XspI CTAG 2 cut(s) 395, 1025
ZrmI AGTACT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.