Rw4G001400

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000144
Physical Location & Seq
Forward (+)
2516282 .. 2517280
999 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G001400.1

Sequence Viewer

Length: 999 bp
ATGGACATTGTGCCCTGGAAGAAACTACCTGACGATGTCATAGAGATGATTGTGCCACACCTGAGTGTACGTGATCGTATACGATTAAGCAGTGTTTGCAAGCCTTGGAGCTCAGTTCCTATGCGCACCGACATCGCTAGTGCTCAACATCAGCTTCCCTGGTTAATACTCCCTCAATCTTGCCATAGCTACTTGAGCTTTTTCGACCTCTGTGAAGGTAAAGTTGGCAAGTTGGACCTGCCTGAACCAGTTCAAGGAGGGTGGTTTCAAGGATCATCAAAAGGTTGGATGATTATGATCAAGGAAAAGGATCTGGATTCTACAATGTTTCTTGTGAATCCAATTTCAGGAGCCCAGCACCAACTTCCACCTTTGAGCACGACTCCATCTTTCCAGAGCTTTGTAGACACAACCGCATGGAAACGCTATGGTGCTTCTGCTTTCTGCTATAGCGTTGTGTTATCTACCTCAGATATTAATTCAGATCAATTTATGGTTGCAGCAACTTTTGGAGATACACTGTGTTTGTTTAGACTGGGGGACAAGAGGTGGAGGTTCATCGAGGTGTTAGATGAAAACCAGGGCATTACAGATTTATTGTTTTCTTCTGGGAAGCTCTACGCCTTGCCTATTAGTGATAATAAGATTGGCGTCATCTCAGCTCCTCGCACCTTAAAATGTGGAGATCATGTATTGGAATTGCAGCTGGTCTATGACAAAAAACCAAGAACCAATGACAGCATGGAAGAATATGCTGACTATCTAATCCAATTACAAGGGACAGTAAGCTCACGTTTGGTAGAGTCAACAACCAAGGATGAAATACTGTTGATCCATCAAGTTCAAGATATATTTGCAAAATGGCATGACAACCATGAGGAGGTGGAGAATGTTGAAGGCAGTGATGATGACGATGACGACAACGAAGATGACAACTTGGAAGGTAATGAGGAGGGAGGAGAAGAGGTTGATGCATTAAAGAACACAGATTTTTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

332

Amino Acids

37.47

Weight (kDa)

4.52

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 6 - 38 2.9e-06 F-box domain
F-box-like PF12937 6 - 38 7.4e-06 F-box-like
Beta-prop_KIB1-4 PF03478 67 - 288 2.3e-24 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 125
Acc36I ACCTGC 1 cut(s) 246
AccI GTMKAC 2 cut(s) 79, 405
AciI CCGC 1 cut(s) 414
AclWI GGATC 3 cut(s) 280, 318, 826
AcyI GRCGYC 1 cut(s) 651
AfaI GTAC 1 cut(s) 69
AfiI CCNNNNNNNGG 3 cut(s) 254, 347, 880
AgsI TTSAA 4 cut(s) 254, 269, 845, 896
AjnI CCWGG 3 cut(s) 14, 158, 579
AjuI GAANNNNNNNTTGG 2 cut(s) 207, 239
AleI CACNNNNGTG 1 cut(s) 63
AluBI AGCT 9 cut(s) 111, 154, 189, 198, 399, 616, 662, 706, 789
AluI AGCT 9 cut(s) 111, 154, 189, 198, 399, 616, 662, 706, 789
Alw21I GWGCWC 3 cut(s) 113, 145, 380
AlwI GGATC 3 cut(s) 280, 318, 826
ApeKI GCWGC 2 cut(s) 500, 703
AseI ATTAAT 1 cut(s) 477
Asp700I GAANNNNTTC 1 cut(s) 249
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 235
AvaII GGWCC 1 cut(s) 235
BaeGI GKGCMC 1 cut(s) 15
BanII GRGCYC 2 cut(s) 113, 355
Bbv12I GWGCWC 3 cut(s) 113, 145, 380
BbvI GCAGC 2 cut(s) 512, 715
BccI CCATC 2 cut(s) 394, 843
BcgI CGANNNNNNTGC 2 cut(s) 115, 149
BciT130I CCWGG 3 cut(s) 16, 160, 581
BclI TGATCA 1 cut(s) 297
BfaI CTAG 1 cut(s) 138
BfmI CTRYAG 1 cut(s) 448
BfuAI ACCTGC 1 cut(s) 246
BisI GCNGC 2 cut(s) 501, 704
BlsI GCNGC 2 cut(s) 502, 705
Bme1390I CCNGG 3 cut(s) 16, 160, 581
Bme18I GGWCC 1 cut(s) 235
BmgT120I GGNCC 1 cut(s) 235
BmiI GGNNCC 1 cut(s) 352
BmrFI CCNGG 3 cut(s) 16, 160, 581
BmrI ACTGGG 1 cut(s) 545
BmsI GCATC 1 cut(s) 961
BmuI ACTGGG 1 cut(s) 545
BplI GAGNNNNNCTC 2 cut(s) 367, 399
BpuEI CTTGAG 1 cut(s) 214
BsaAI YACGTR 1 cut(s) 71
BsaBI GATNNNNATC 1 cut(s) 296
BsaHI GRCGYC 1 cut(s) 651
BsaJI CCNNGG 5 cut(s) 14, 104, 158, 580, 813
BsaXI ACNNNNNCTCC 4 cut(s) 675, 705, 951, 981
Bsc4I CCNNNNNNNGG 3 cut(s) 254, 347, 880
Bse1I ACTGG 2 cut(s) 248, 540
Bse8I GATNNNNATC 1 cut(s) 296
BseBI CCWGG 3 cut(s) 16, 160, 581
BseDI CCNNGG 5 cut(s) 14, 104, 158, 580, 813
BseGI GGATG 2 cut(s) 294, 823
BseJI GATNNNNATC 1 cut(s) 296
BseLI CCNNNNNNNGG 3 cut(s) 254, 347, 880
BseMII CTCAG 4 cut(s) 53, 126, 483, 672
BseNI ACTGG 2 cut(s) 248, 540
BseRI GAGGAG 4 cut(s) 654, 893, 965, 972
BseSI GKGCMC 1 cut(s) 15
BseXI GCAGC 2 cut(s) 512, 715
BseYI CCCAGC 1 cut(s) 354
BsiHKAI GWGCWC 3 cut(s) 113, 145, 380
BslFI GGGAC 2 cut(s) 554, 793
BslI CCNNNNNNNGG 3 cut(s) 254, 347, 880
BsmFI GGGAC 2 cut(s) 554, 793
Bsp1286I GDGCHC 5 cut(s) 15, 113, 145, 355, 380
Bsp143I GATC 7 cut(s) 73, 272, 297, 310, 484, 685, 831
BspACI CCGC 1 cut(s) 414
BspCNI CTCAG 4 cut(s) 54, 125, 482, 671
BspLI GGNNCC 1 cut(s) 352
BspMI ACCTGC 1 cut(s) 246
BspPI GGATC 3 cut(s) 280, 318, 826
BsrI ACTGG 2 cut(s) 248, 540
BssECI CCNNGG 5 cut(s) 14, 104, 158, 580, 813
BssMI GATC 7 cut(s) 73, 272, 297, 310, 484, 685, 831
BssNAI GTATAC 1 cut(s) 80
BssNI GRCGYC 1 cut(s) 651
BssT1I CCWWGG 2 cut(s) 104, 813
Bst1107I GTATAC 1 cut(s) 80
Bst2UI CCWGG 3 cut(s) 16, 160, 581
Bst4CI ACNGT 3 cut(s) 522, 784, 828
Bst6I CTCTTC 1 cut(s) 957
BstACI GRCGYC 1 cut(s) 651
BstAPI GCANNNNNTGC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 71
BstC8I GCNNGC 1 cut(s) 101
BstDEI CTNAG 4 cut(s) 62, 112, 469, 658
BstF5I GGATG 2 cut(s) 294, 823
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 7 cut(s) 76, 275, 300, 313, 487, 688, 834
BstMBI GATC 7 cut(s) 73, 272, 297, 310, 484, 685, 831
BstMWI GCNNNNNNNGC 2 cut(s) 96, 195
BstNI CCWGG 3 cut(s) 16, 160, 581
BstSCI CCNGG 3 cut(s) 14, 158, 579
BstSFI CTRYAG 1 cut(s) 448
BstSLI GKGCMC 1 cut(s) 15
BstV1I GCAGC 2 cut(s) 512, 715
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BstZ17I GTATAC 1 cut(s) 80
BtgZI GCGATG 1 cut(s) 118
BtsCI GGATG 2 cut(s) 294, 823
BtsI GCAGTG 2 cut(s) 97, 907
BtsIMutI CAGTG 3 cut(s) 97, 518, 907
BveI ACCTGC 1 cut(s) 246
Cac8I GCNNGC 1 cut(s) 101
CfoI GCGC 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 235
CseI GACGC 1 cut(s) 640
Csp6I GTAC 1 cut(s) 68
CspCI CAANNNNNGTGG 2 cut(s) 242, 277
CviAII CATG 5 cut(s) 417, 689, 742, 866, 875
CviQI GTAC 1 cut(s) 68
DdeI CTNAG 4 cut(s) 62, 112, 469, 658
DpnI GATC 7 cut(s) 75, 274, 299, 312, 486, 687, 833
DpnII GATC 7 cut(s) 73, 272, 297, 310, 484, 685, 831
Eam1104I CTCTTC 1 cut(s) 957
EarI CTCTTC 1 cut(s) 957
Ecl136II GAGCTC 1 cut(s) 111
Eco130I CCWWGG 2 cut(s) 104, 813
Eco24I GRGCYC 2 cut(s) 113, 355
Eco47I GGWCC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 111
EcoICRI GAGCTC 1 cut(s) 111
EcoRII CCWGG 3 cut(s) 14, 158, 579
EcoT14I CCWWGG 2 cut(s) 104, 813
EcoT22I ATGCAT 1 cut(s) 976
EcoT38I GRGCYC 2 cut(s) 113, 355
ErhI CCWWGG 2 cut(s) 104, 813
FaeI CATG 5 cut(s) 420, 692, 745, 869, 878
FaqI GGGAC 2 cut(s) 554, 793
FatI CATG 5 cut(s) 416, 688, 741, 865, 874
FbaI TGATCA 1 cut(s) 297
FblI GTMKAC 2 cut(s) 79, 405
Fnu4HI GCNGC 2 cut(s) 501, 704
FokI GGATG 2 cut(s) 301, 830
FriOI GRGCYC 2 cut(s) 113, 355
Fsp4HI GCNGC 2 cut(s) 501, 704
FspAI RTGCGCAY 1 cut(s) 125
FspBI CTAG 1 cut(s) 138
FspI TGCGCA 1 cut(s) 125
GlaI GCGC 1 cut(s) 125
GluI GCNGC 2 cut(s) 501, 704
GsaI CCCAGC 1 cut(s) 358
HgaI GACGC 1 cut(s) 640
HhaI GCGC 1 cut(s) 126
Hin1I GRCGYC 1 cut(s) 651
Hin1II CATG 5 cut(s) 420, 692, 745, 869, 878
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HincII GTYRAC 1 cut(s) 807
HindII GTYRAC 1 cut(s) 807
HinfI GANTC 4 cut(s) 317, 337, 382, 803
Hpy166II GTNNAC 4 cut(s) 68, 80, 406, 807
Hpy188I TCNGA 2 cut(s) 472, 484
Hpy188III TCNNGA 4 cut(s) 314, 348, 394, 845
Hpy8I GTNNAC 4 cut(s) 68, 80, 406, 807
HpyAV CCTTC 3 cut(s) 209, 890, 935
HpyCH4III ACNGT 3 cut(s) 522, 784, 828
HpyCH4IV ACGT 2 cut(s) 70, 793
HpyCH4V TGCA 5 cut(s) 99, 500, 703, 857, 974
HpyF10VI GCNNNNNNNGC 2 cut(s) 96, 195
HpyF3I CTNAG 4 cut(s) 62, 112, 469, 658
HpySE526I ACGT 2 cut(s) 70, 793
Hsp92I GRCGYC 1 cut(s) 651
Hsp92II CATG 5 cut(s) 420, 692, 745, 869, 878
HspAI GCGC 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 297
Kzo9I GATC 7 cut(s) 73, 272, 297, 310, 484, 685, 831
LmnI GCTCC 3 cut(s) 108, 350, 667
Lsp1109I GCAGC 2 cut(s) 512, 715
LweI GCATC 1 cut(s) 961
MaeI CTAG 1 cut(s) 138
MaeII ACGT 2 cut(s) 70, 793
MalI GATC 7 cut(s) 75, 274, 299, 312, 486, 687, 833
MboI GATC 7 cut(s) 73, 272, 297, 310, 484, 685, 831
MboII GAAGA 5 cut(s) 31, 597, 758, 938, 974
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 5 cut(s) 15, 113, 145, 355, 380
MluCI AATT 5 cut(s) 342, 478, 488, 698, 770
MlyI GAGTC 2 cut(s) 376, 812
MmeI TCCRAC 2 cut(s) 213, 266
Mph1103I ATGCAT 1 cut(s) 976
MroXI GAANNNNTTC 1 cut(s) 249
MseI TTAA 6 cut(s) 86, 164, 477, 674, 977, 997
MslI CAYNNNNRTG 3 cut(s) 44, 63, 563
MspA1I CMGCKG 1 cut(s) 706
MspR9I CCNGG 3 cut(s) 16, 160, 581
MvaI CCWGG 3 cut(s) 16, 160, 581
MwoI GCNNNNNNNGC 2 cut(s) 96, 195
NdeII GATC 7 cut(s) 73, 272, 297, 310, 484, 685, 831
NlaIII CATG 5 cut(s) 420, 692, 745, 869, 878
NlaIV GGNNCC 1 cut(s) 352
NsbI TGCGCA 1 cut(s) 125
NsiI ATGCAT 1 cut(s) 976
OliI CACNNNNGTG 1 cut(s) 63
PdmI GAANNNNTTC 1 cut(s) 249
PfeI GAWTC 2 cut(s) 317, 337
PflFI GACNNNGTC 1 cut(s) 35
PkrI GCNGC 2 cut(s) 502, 705
PleI GAGTC 2 cut(s) 376, 811
PpsI GAGTC 2 cut(s) 376, 811
Ppu21I YACGTR 1 cut(s) 71
PshBI ATTAAT 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 113
Psp6I CCWGG 3 cut(s) 14, 158, 579
PspFI CCCAGC 1 cut(s) 354
PspGI CCWGG 3 cut(s) 14, 158, 579
PspN4I GGNNCC 1 cut(s) 352
PspPI GGNCC 1 cut(s) 235
PsuI RGATCY 1 cut(s) 310
PsyI GACNNNGTC 1 cut(s) 35
PvuII CAGCTG 1 cut(s) 706
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
RseI CAYNNNNRTG 3 cut(s) 44, 63, 563
SacI GAGCTC 1 cut(s) 113
SaqAI TTAA 6 cut(s) 86, 164, 477, 674, 977, 997
SatI GCNGC 2 cut(s) 501, 704
Sau3AI GATC 7 cut(s) 73, 272, 297, 310, 484, 685, 831
Sau96I GGNCC 1 cut(s) 235
SchI GAGTC 2 cut(s) 376, 812
ScrFI CCNGG 3 cut(s) 16, 160, 581
SduI GDGCHC 5 cut(s) 15, 113, 145, 355, 380
SfaNI GCATC 1 cut(s) 961
SfcI CTRYAG 1 cut(s) 448
SinI GGWCC 1 cut(s) 235
SmiMI CAYNNNNRTG 3 cut(s) 44, 63, 563
SmlI CTYRAG 1 cut(s) 193
SmoI CTYRAG 1 cut(s) 193
Sse9I AATT 5 cut(s) 342, 478, 488, 698, 770
SsiI CCGC 1 cut(s) 414
SspMI CTAG 1 cut(s) 138
SstI GAGCTC 1 cut(s) 113
StyD4I CCNGG 3 cut(s) 14, 158, 579
StyI CCWWGG 2 cut(s) 104, 813
TaaI ACNGT 3 cut(s) 522, 784, 828
TaiI ACGT 2 cut(s) 73, 796
TaqI TCGA 2 cut(s) 204, 561
TasI AATT 5 cut(s) 342, 478, 488, 698, 770
TfiI GAWTC 2 cut(s) 317, 337
Tru1I TTAA 6 cut(s) 86, 164, 477, 674, 977, 997
Tru9I TTAA 6 cut(s) 86, 164, 477, 674, 977, 997
TscAI CASTG 3 cut(s) 97, 525, 907
TseI GCWGC 2 cut(s) 500, 703
TspDTI ATGAA 3 cut(s) 547, 588, 834
TspRI CASTG 3 cut(s) 97, 525, 907
Tth111I GACNNNGTC 1 cut(s) 35
VpaK11BI GGWCC 1 cut(s) 235
VspI ATTAAT 1 cut(s) 477
XcmI CCANNNNNNNNNTGG 1 cut(s) 739
XmiI GTMKAC 2 cut(s) 79, 405
XmnI GAANNNNTTC 1 cut(s) 249
XspI CTAG 1 cut(s) 138
Zsp2I ATGCAT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.