Rorug05G0553400

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000144
Physical Location & Seq
Reverse (-)
74118342 .. 74124233
5892 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0553400.1

Sequence Viewer

Length: 2250 bp
ATGAGCGGCGGTGGAGAGACTAGGGTTTCAATCCCAGACAATGTACACAGCACGATCCAAAACATCAGAGAGATCACTAAGAAGCTTCACAGCGACGATGAAATCTATGCTGTGCTCAAGGAGTGCTCTATGGATCCCAACGAGACCGCCCAGAAGCTCTTGTATTTAGATACATTTCATGAGGTCAAAAAGAGACGTGATCGGAGAAAGGAGAACCTGAAAGGCAGGGCATCTGAAGATTCGAGGACAGCACCAGGTGCGCACAGGCGAATGGTCAGGGGTGGTGGCCAAGGGAATTATTCTTCTGATGCTGGTGGTGGGAGGAATTCTGCTGCTCGGAGGGAAAATGGAGCCAGTCATGTTGCAGATGGAGGTTCTCTGTCCTCTTTTATGCCAGGTCAGCAGAAAACTAAGAACAATGCTGCACCTCCAATCGCAAAGGCTTCAATTGGCATTCCAAACGGGATTAAAAACCTGTCAAATGGCTCTAGTCATGTTTCTGCTCCAAAATCCTCTGCCGGTGTAGATGCAAACAAGTTGGGAGCTACAGCACCTCGTTATGCTGTCGTTGTGGCATCTACACCTGTTGTTGGCTTAAAGGATCAACGCAAGGCCCCAACAACTACATCTGATCATCTTCCAAATTCTGCCACTTCTGCATCTGATTCTGGTGTTTACTCATCTGCTTCAGATCCTTCCTTATCAACTCATATACCAGAAAACAAAGATGTTTCTGGTGAATCTGTTGTCATAGAGTTGTCAAAGAGTGAAAATGTTGCCTCCAAATCTGATAATCACGTGATCAAAGAAAAGACTCAAAGTGACAATGCTAAGGCAATTGGAAAGAATCGGCATACTAAGTCGTCAGAACCCCCTTCCTCAACTCATGATGGTTCTCAGGCCATTCTGCCTTCTAATTCTGATGGAGAATCACAGATAGAGTCGACTGATGCTTTGAAAGTGGCCAAACTGGAGGTTGAAACCGTTGCTGTTAAACCTTCTTCTCAGTTGCCACCTGACTCGAGTCTCTCAGATACCAAACTCGTTACTTTTCCAAATCATATCAAGGTCCCTGAATCTTTAAGAAATGTTTTGACTTTTGGAAGTATTAATTCTACTTTTGGAACGAGTGTTGACTGCGTTAACGGTACTGGTGGCAATGGTTCTACGGGCGGTGTAGAATCTTCTCAGGATACTGATGAAACTGCTAAAGAGCCTTCTCCTAGCAATAACAGTGTAGCCTCATCTGTTCAAGGAGATTTTTCAGAGAATCCACTGCCTTCACCAAATGTTCTTGAAAAGTTACAACCACCTGAGGGTAATGTCTCTTCAGAGAGTAATGTCCCTTCAGCCCCTGACTCAGACTCCAAAAGTGAACTGTTGAAGGATTTGCAGTTGCCTCCAGAAGTTCCACATAACCTCACTGCTCTAAATGGCCCCAGCTATAGTCTTGGTTTTTTGCCACCGATGCTAGGAGGCCAGGTTTTACAAGTGGAAGGACATGATAATCAGGCACCTGAAGCACCTCGGGTTTCGAACTTTGGTGGAAATTCTGTTGGTGTTCCCAGCCCAAATACAACCCCACCCCTGCCAAGCTCCATAGCTGCTTCTCCACAGATTCCTGTTTTCAGGCATGCATATCCCGCGAATTATTTCCCATATGGCTATCTCCCGCCGTATTATCTGCCACCAATGCACCAGTTTTTAAGCCCCAATGGCTTTCCTCCACAGCCTTCAGCATATCTACCACCAACAGCACCAGCTGCTGCTGGGATTAAATATCCTCCTCCGCAGTTCAAGGCAGGGAGCAATGCTGGAAATCCACCACAGTATAACATTCAATCCGGTGGTTCATTCATAACCACCCCTGCTGGTTATGCTCCTGGTTCAGCAGTTGCATCTGGAAACTCAGTTGGTAACACTGAAGATCTTGGAGCATCTCAATCGAAAGAAAGTCATATCTACACTACAGGACAGCTGACTGAGGGTTCAACTGTTTGGATTCATACTCCTGGACAAGATATCAGTTCTCTGTACAACCTTCCTCAGGGGCAACGCTTCGCGTTCTCGCCTGTCCATGCTGGTCATGGTGCCATGACTGGACTTTATCCACCAGGGCAAACGATGGCTTCTCCAACATATCTGCAACAGTCCCAGGCTGCGGCTGGAGCTGCTGAGACCATAGGACCCCCACCTGCTGCTTATCAGCAGCCTCAACATGCACAGATGAATTGGAACAGTAGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

749

Amino Acids

78.29

Weight (kDa)

6.1

Isoelectric Point (pI)

56.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GIP1_N PF06972 11 - 71 2e-30 GBF-interacting protein 1 N-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2203
Acc16I TGCGCA 1 cut(s) 261
Acc36I ACCTGC 1 cut(s) 2203
AccB1I GGYRCC 2 cut(s) 1513, 2090
AccBSI CCGCTC 1 cut(s) 6
AccI GTMKAC 1 cut(s) 944
AccII CGCG 2 cut(s) 1646, 2063
AciI CCGC 8 cut(s) 6, 9, 147, 1173, 1644, 1673, 1790, 2162
AclWI GGATC 5 cut(s) 49, 128, 141, 609, 686
AcoI YGGCCR 2 cut(s) 286, 963
AcsI RAATTY 3 cut(s) 325, 643, 1549
AcuI CTGAAG 7 cut(s) 255, 672, 1314, 1332, 1539, 1719, 1944
AcvI CACGTG 1 cut(s) 799
AdeI CACNNNGTG 1 cut(s) 257
AfaI GTAC 3 cut(s) 45, 1150, 2036
AfiI CCNNNNNNNGG 5 cut(s) 590, 1316, 1472, 2047, 2161
AjiI CACGTC 1 cut(s) 197
AjnI CCWGG 7 cut(s) 253, 394, 1479, 1882, 2011, 2113, 2154
AluBI AGCT 9 cut(s) 85, 157, 545, 1443, 1596, 1604, 1763, 1978, 2171
AluI AGCT 9 cut(s) 85, 157, 545, 1443, 1596, 1604, 1763, 1978, 2171
Alw21I GWGCWC 2 cut(s) 117, 128
Alw26I GTCTC 6 cut(s) 11, 137, 187, 1031, 1330, 2171
AlwI GGATC 5 cut(s) 49, 128, 141, 609, 686
AlwNI CAGNNNCTG 2 cut(s) 1355, 1766
Ama87I CYCGRG 2 cut(s) 1021, 1527
AoxI GGCC 6 cut(s) 286, 612, 900, 963, 1435, 1477
ApeKI GCWGC 9 cut(s) 332, 422, 1604, 1763, 1766, 2159, 2171, 2198, 2209
ApoI RAATTY 3 cut(s) 325, 643, 1549
ArsI GACNNNNNNTTYG 2 cut(s) 937, 969
AseI ATTAAT 1 cut(s) 1110
Asp700I GAANNNNTTC 1 cut(s) 1652
AspLEI GCGC 1 cut(s) 262
AspS9I GGNCC 4 cut(s) 613, 1069, 1436, 2186
AsuHPI GGTGA 2 cut(s) 749, 1275
AsuII TTCGAA 1 cut(s) 1535
AvaI CYCGRG 2 cut(s) 1021, 1527
AvaII GGWCC 2 cut(s) 1069, 2186
AxyI CCTNAGG 2 cut(s) 1314, 2046
BalI TGGCCA 2 cut(s) 288, 965
BamHI GGATCC 1 cut(s) 133
BanI GGYRCC 2 cut(s) 1513, 2090
BarI GAAGNNNNNNTAC 4 cut(s) 1312, 1330, 1344, 1362
BbrPI CACGTG 1 cut(s) 799
Bbv12I GWGCWC 2 cut(s) 117, 128
BbvI GCAGC 9 cut(s) 319, 409, 1591, 1750, 1753, 2146, 2158, 2185, 2221
BccI CCATC 4 cut(s) 362, 884, 917, 2119
BceAI ACGGC 1 cut(s) 1660
BcgI CGANNNNNNTGC 2 cut(s) 1926, 1960
BciT130I CCWGG 7 cut(s) 255, 396, 1481, 1884, 2013, 2115, 2156
BciVI GTATCC 1 cut(s) 1186
BclI TGATCA 2 cut(s) 631, 801
BcoDI GTCTC 6 cut(s) 11, 137, 187, 1031, 1330, 2171
BfaI CTAG 4 cut(s) 21, 489, 1224, 1472
BfmI CTRYAG 3 cut(s) 546, 1444, 1968
BfuAI ACCTGC 1 cut(s) 2203
BfuI GTATCC 1 cut(s) 1186
BglI GCCNNNNNGGC 1 cut(s) 1716
BglII AGATCT 1 cut(s) 1927
Bme1390I CCNGG 7 cut(s) 255, 396, 1481, 1884, 2013, 2115, 2156
Bme18I GGWCC 2 cut(s) 1069, 2186
BmeT110I CYCGRG 2 cut(s) 1021, 1527
BmgBI CACGTC 1 cut(s) 197
BmgT120I GGNCC 4 cut(s) 613, 1069, 1436, 2186
BmiI GGNNCC 8 cut(s) 135, 352, 615, 1071, 1438, 1515, 2092, 2188
BmrFI CCNGG 7 cut(s) 255, 396, 1481, 1884, 2013, 2115, 2156
BmsI GCATC 9 cut(s) 239, 298, 517, 584, 668, 940, 1458, 1907, 1946
BoxI GACNNNNGTC 1 cut(s) 1023
BpmI CTGGAG 3 cut(s) 992, 1386, 2187
Bpu10I CCTNAGC 1 cut(s) 831
Bpu14I TTCGAA 1 cut(s) 1535
BpuEI CTTGAG 1 cut(s) 101
BsaAI YACGTR 1 cut(s) 799
BsaI GGTCTC 2 cut(s) 137, 2171
BsaJI CCNNGG 4 cut(s) 289, 1526, 2114, 2154
BsaWI WCCGGW 1 cut(s) 1844
BsaXI ACNNNNNCTCC 4 cut(s) 1349, 1379, 1467, 1497
Bsc4I CCNNNNNNNGG 5 cut(s) 590, 1316, 1472, 2047, 2161
Bse118I RCCGGY 1 cut(s) 518
Bse1I ACTGG 5 cut(s) 354, 975, 1156, 1699, 2104
Bse21I CCTNAGG 2 cut(s) 1314, 2046
Bse3DI GCAATG 2 cut(s) 1165, 1816
BseBI CCWGG 7 cut(s) 255, 396, 1481, 1884, 2013, 2115, 2156
BseDI CCNNGG 4 cut(s) 289, 1526, 2114, 2154
BseLI CCNNNNNNNGG 5 cut(s) 590, 1316, 1472, 2047, 2161
BseMI GCAATG 2 cut(s) 1165, 1816
BseNI ACTGG 5 cut(s) 354, 975, 1156, 1699, 2104
BseRI GAGGAG 1 cut(s) 1776
BseXI GCAGC 9 cut(s) 319, 409, 1591, 1750, 1753, 2146, 2158, 2185, 2221
BseYI CCCAGC 3 cut(s) 1439, 1565, 1769
BsgI GTGCAG 1 cut(s) 408
Bsh1236I CGCG 2 cut(s) 1646, 2063
BshFI GGCC 6 cut(s) 288, 614, 902, 965, 1437, 1479
BshNI GGYRCC 2 cut(s) 1513, 2090
BsiHKAI GWGCWC 2 cut(s) 117, 128
BsiHKCI CYCGRG 2 cut(s) 1021, 1527
BsiSI CCGG 2 cut(s) 519, 1845
BslFI GGGAC 3 cut(s) 1055, 1328, 2137
BslI CCNNNNNNNGG 5 cut(s) 590, 1316, 1472, 2047, 2161
BsmAI GTCTC 6 cut(s) 11, 137, 187, 1031, 1330, 2171
BsmBI CGTCTC 1 cut(s) 187
BsmFI GGGAC 3 cut(s) 1055, 1328, 2137
BsmI GAATGC 1 cut(s) 453
BsnI GGCC 6 cut(s) 288, 614, 902, 965, 1437, 1479
Bso31I GGTCTC 2 cut(s) 137, 2171
BsoBI CYCGRG 2 cut(s) 1021, 1527
Bsp119I TTCGAA 1 cut(s) 1535
Bsp1286I GDGCHC 2 cut(s) 117, 128
Bsp1407I TGTACA 2 cut(s) 43, 2034
Bsp143I GATC 9 cut(s) 54, 72, 133, 199, 601, 631, 691, 801, 1927
BspACI CCGC 8 cut(s) 6, 9, 147, 1173, 1644, 1673, 1790, 2162
BspANI GGCC 6 cut(s) 288, 614, 902, 965, 1437, 1479
BspFNI CGCG 2 cut(s) 1646, 2063
BspHI TCATGA 2 cut(s) 178, 886
BspLI GGNNCC 8 cut(s) 135, 352, 615, 1071, 1438, 1515, 2092, 2188
BspMI ACCTGC 1 cut(s) 2203
BspPI GGATC 5 cut(s) 49, 128, 141, 609, 686
BspT104I TTCGAA 1 cut(s) 1535
BspT107I GGYRCC 2 cut(s) 1513, 2090
BspTNI GGTCTC 2 cut(s) 137, 2171
BsrBI CCGCTC 1 cut(s) 6
BsrDI GCAATG 2 cut(s) 1165, 1816
BsrFI RCCGGY 1 cut(s) 518
BsrGI TGTACA 2 cut(s) 43, 2034
BsrI ACTGG 5 cut(s) 354, 975, 1156, 1699, 2104
BssAI RCCGGY 1 cut(s) 518
BssECI CCNNGG 4 cut(s) 289, 1526, 2114, 2154
BssMI GATC 9 cut(s) 54, 72, 133, 199, 601, 631, 691, 801, 1927
BssT1I CCWWGG 1 cut(s) 289
Bst2UI CCWGG 7 cut(s) 255, 396, 1481, 1884, 2013, 2115, 2156
Bst4CI ACNGT 8 cut(s) 985, 1148, 1235, 1380, 1830, 1996, 2151, 2240
Bst6I CTCTTC 1 cut(s) 1333
BstAPI GCANNNNNTGC 2 cut(s) 257, 1763
BstAUI TGTACA 2 cut(s) 43, 2034
BstBAI YACGTR 1 cut(s) 799
BstBI TTCGAA 1 cut(s) 1535
BstC8I GCNNGC 1 cut(s) 1635
BstENI CCTNNNNNAGG 1 cut(s) 2045
BstFNI CGCG 2 cut(s) 1646, 2063
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 9 cut(s) 57, 75, 136, 202, 604, 634, 694, 804, 1930
BstMAI GTCTC 6 cut(s) 11, 137, 187, 1031, 1330, 2171
BstMBI GATC 9 cut(s) 54, 72, 133, 199, 601, 631, 691, 801, 1927
BstNI CCWGG 7 cut(s) 255, 396, 1481, 1884, 2013, 2115, 2156
BstNSI RCATGY 2 cut(s) 1637, 2222
BstPAI GACNNNNGTC 1 cut(s) 1023
BstSCI CCNGG 7 cut(s) 253, 394, 1479, 1882, 2011, 2113, 2154
BstSFI CTRYAG 3 cut(s) 546, 1444, 1968
BstUI CGCG 2 cut(s) 1646, 2063
BstV1I GCAGC 9 cut(s) 319, 409, 1591, 1750, 1753, 2146, 2158, 2185, 2221
BstX2I RGATCY 3 cut(s) 133, 691, 1927
BstYI RGATCY 3 cut(s) 133, 691, 1927
Bsu36I CCTNAGG 2 cut(s) 1314, 2046
BsuI GTATCC 1 cut(s) 1186
BsuRI GGCC 6 cut(s) 288, 614, 902, 965, 1437, 1479
BtrI CACGTC 1 cut(s) 197
BtsI GCAGTG 2 cut(s) 1274, 1422
BtsIMutI CAGTG 4 cut(s) 1240, 1274, 1422, 1920
BveI ACCTGC 1 cut(s) 2203
Cac8I GCNNGC 1 cut(s) 1635
CaiI CAGNNNCTG 2 cut(s) 1355, 1766
CciI TCATGA 2 cut(s) 178, 886
CfoI GCGC 1 cut(s) 262
Cfr10I RCCGGY 1 cut(s) 518
Cfr13I GGNCC 4 cut(s) 613, 1069, 1436, 2186
CsiI ACCWGGT 1 cut(s) 253
Csp6I GTAC 3 cut(s) 44, 1149, 2035
CviQI GTAC 3 cut(s) 44, 1149, 2035
DpnI GATC 9 cut(s) 56, 74, 135, 201, 603, 633, 693, 803, 1929
DpnII GATC 9 cut(s) 54, 72, 133, 199, 601, 631, 691, 801, 1927
DraIII CACNNNGTG 1 cut(s) 257
EaeI YGGCCR 2 cut(s) 286, 963
Eam1104I CTCTTC 1 cut(s) 1333
EarI CTCTTC 1 cut(s) 1333
Eco130I CCWWGG 1 cut(s) 289
Eco31I GGTCTC 2 cut(s) 137, 2171
Eco32I GATATC 1 cut(s) 2023
Eco47I GGWCC 2 cut(s) 1069, 2186
Eco57I CTGAAG 7 cut(s) 255, 672, 1314, 1332, 1539, 1719, 1944
Eco72I CACGTG 1 cut(s) 799
Eco81I CCTNAGG 2 cut(s) 1314, 2046
Eco88I CYCGRG 2 cut(s) 1021, 1527
EcoNI CCTNNNNNAGG 1 cut(s) 2045
EcoO109I RGGNCCY 3 cut(s) 613, 1069, 2186
EcoRI GAATTC 1 cut(s) 325
EcoRII CCWGG 7 cut(s) 253, 394, 1479, 1882, 2011, 2113, 2154
EcoRV GATATC 1 cut(s) 2023
EcoT14I CCWWGG 1 cut(s) 289
EcoT22I ATGCAT 1 cut(s) 1639
ErhI CCWWGG 1 cut(s) 289
Esp3I CGTCTC 1 cut(s) 187
FaqI GGGAC 3 cut(s) 1055, 1328, 2137
FauI CCCGC 2 cut(s) 1651, 1680
FauNDI CATATG 1 cut(s) 1660
FbaI TGATCA 2 cut(s) 631, 801
FblI GTMKAC 1 cut(s) 944
FspAI RTGCGCAY 1 cut(s) 261
FspBI CTAG 4 cut(s) 21, 489, 1224, 1472
FspI TGCGCA 1 cut(s) 261
GlaI GCGC 1 cut(s) 261
GsaI CCCAGC 3 cut(s) 1443, 1569, 1773
GsuI CTGGAG 3 cut(s) 992, 1386, 2187
HaeIII GGCC 6 cut(s) 288, 614, 902, 965, 1437, 1479
HapII CCGG 2 cut(s) 519, 1845
HhaI GCGC 1 cut(s) 262
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HincII GTYRAC 3 cut(s) 945, 1135, 1144
HindII GTYRAC 3 cut(s) 945, 1135, 1144
HindIII AAGCTT 1 cut(s) 83
HpaI GTTAAC 1 cut(s) 1144
HpaII CCGG 2 cut(s) 519, 1845
HphI GGTGA 2 cut(s) 749, 1275
Hpy166II GTNNAC 6 cut(s) 46, 676, 945, 1135, 1144, 1376
Hpy188III TCNNGA 6 cut(s) 179, 887, 1190, 1295, 1403, 1902
Hpy8I GTNNAC 6 cut(s) 46, 676, 945, 1135, 1144, 1376
Hpy99I CGWCG 1 cut(s) 98
HpyCH4III ACNGT 8 cut(s) 985, 1148, 1235, 1380, 1830, 1996, 2151, 2240
HpyCH4IV ACGT 2 cut(s) 196, 798
HpySE526I ACGT 2 cut(s) 196, 798
HspAI GCGC 1 cut(s) 260
Ksp22I TGATCA 2 cut(s) 631, 801
KspAI GTTAAC 1 cut(s) 1144
Kzo9I GATC 9 cut(s) 54, 72, 133, 199, 601, 631, 691, 801, 1927
LmnI GCTCC 8 cut(s) 350, 508, 542, 1601, 1806, 1885, 1934, 2168
Lsp1109I GCAGC 9 cut(s) 319, 409, 1591, 1750, 1753, 2146, 2158, 2185, 2221
LweI GCATC 9 cut(s) 239, 298, 517, 584, 668, 940, 1458, 1907, 1946
MabI ACCWGGT 1 cut(s) 253
MaeI CTAG 4 cut(s) 21, 489, 1224, 1472
MaeII ACGT 2 cut(s) 196, 798
MaeIII GTNAC 4 cut(s) 821, 1045, 1302, 1916
MalI GATC 9 cut(s) 56, 74, 135, 201, 603, 633, 693, 803, 1929
MbiI CCGCTC 1 cut(s) 6
MboI GATC 9 cut(s) 54, 72, 133, 199, 601, 631, 691, 801, 1927
MboII GAAGA 7 cut(s) 248, 294, 629, 993, 1176, 1320, 1937
MfeI CAATTG 2 cut(s) 447, 837
MflI RGATCY 3 cut(s) 133, 691, 1927
MhlI GDGCHC 2 cut(s) 117, 128
MlsI TGGCCA 2 cut(s) 288, 965
MluNI TGGCCA 2 cut(s) 288, 965
MlyI GAGTC 6 cut(s) 808, 950, 1013, 1033, 1352, 1358
MmeI TCCRAC 1 cut(s) 2159
Mox20I TGGCCA 2 cut(s) 288, 965
Mph1103I ATGCAT 1 cut(s) 1639
MroXI GAANNNNTTC 1 cut(s) 1652
MscI TGGCCA 2 cut(s) 288, 965
MseI TTAA 8 cut(s) 468, 596, 993, 1082, 1110, 1143, 1706, 1776
Msp20I TGGCCA 2 cut(s) 288, 965
MspA1I CMGCKG 2 cut(s) 1763, 1978
MspI CCGG 2 cut(s) 519, 1845
MspR9I CCNGG 7 cut(s) 255, 396, 1481, 1884, 2013, 2115, 2156
MunI CAATTG 2 cut(s) 447, 837
Mva1269I GAATGC 1 cut(s) 453
MvaI CCWGG 7 cut(s) 255, 396, 1481, 1884, 2013, 2115, 2156
MvnI CGCG 2 cut(s) 1646, 2063
NdeI CATATG 1 cut(s) 1660
NdeII GATC 9 cut(s) 54, 72, 133, 199, 601, 631, 691, 801, 1927
NlaIV GGNNCC 8 cut(s) 135, 352, 615, 1071, 1438, 1515, 2092, 2188
NmuCI GTSAC 1 cut(s) 821
NsbI TGCGCA 1 cut(s) 261
NsiI ATGCAT 1 cut(s) 1639
NspI RCATGY 2 cut(s) 1637, 2222
NspV TTCGAA 1 cut(s) 1535
PaeI GCATGC 1 cut(s) 1637
PaeR7I CTCGAG 1 cut(s) 1021
PagI TCATGA 2 cut(s) 178, 886
PaqCI CACCTGC 1 cut(s) 2203
PctI GAATGC 1 cut(s) 453
PdmI GAANNNNTTC 1 cut(s) 1652
PfoI TCCNGGA 1 cut(s) 2011
PleI GAGTC 6 cut(s) 808, 949, 1013, 1032, 1352, 1358
PmaCI CACGTG 1 cut(s) 799
PmlI CACGTG 1 cut(s) 799
PpsI GAGTC 6 cut(s) 808, 949, 1013, 1032, 1352, 1358
Ppu21I YACGTR 1 cut(s) 799
PpuMI RGGWCCY 2 cut(s) 1069, 2186
PshAI GACNNNNGTC 1 cut(s) 1023
PshBI ATTAAT 1 cut(s) 1110
Psp5II RGGWCCY 2 cut(s) 1069, 2186
Psp6I CCWGG 7 cut(s) 253, 394, 1479, 1882, 2011, 2113, 2154
PspCI CACGTG 1 cut(s) 799
PspFI CCCAGC 3 cut(s) 1439, 1565, 1769
PspGI CCWGG 7 cut(s) 253, 394, 1479, 1882, 2011, 2113, 2154
PspN4I GGNNCC 8 cut(s) 135, 352, 615, 1071, 1438, 1515, 2092, 2188
PspPI GGNCC 4 cut(s) 613, 1069, 1436, 2186
PspPPI RGGWCCY 2 cut(s) 1069, 2186
PspXI VCTCGAGB 1 cut(s) 1021
PstNI CAGNNNCTG 2 cut(s) 1355, 1766
PsuI RGATCY 3 cut(s) 133, 691, 1927
PvuII CAGCTG 2 cut(s) 1763, 1978
RsaI GTAC 3 cut(s) 45, 1150, 2036
RsaNI GTAC 3 cut(s) 44, 1149, 2035
SalI GTCGAC 1 cut(s) 943
SaqAI TTAA 8 cut(s) 468, 596, 993, 1082, 1110, 1143, 1706, 1776
Sau3AI GATC 9 cut(s) 54, 72, 133, 199, 601, 631, 691, 801, 1927
Sau96I GGNCC 4 cut(s) 613, 1069, 1436, 2186
SchI GAGTC 6 cut(s) 808, 950, 1013, 1033, 1352, 1358
ScrFI CCNGG 7 cut(s) 255, 396, 1481, 1884, 2013, 2115, 2156
SduI GDGCHC 2 cut(s) 117, 128
SexAI ACCWGGT 1 cut(s) 253
SfaNI GCATC 9 cut(s) 239, 298, 517, 584, 668, 940, 1458, 1907, 1946
SfcI CTRYAG 3 cut(s) 546, 1444, 1968
Sfr274I CTCGAG 1 cut(s) 1021
SfuI TTCGAA 1 cut(s) 1535
SinI GGWCC 2 cut(s) 1069, 2186
SlaI CTCGAG 1 cut(s) 1021
SmlI CTYRAG 2 cut(s) 116, 1021
SmoI CTYRAG 2 cut(s) 116, 1021
SphI GCATGC 1 cut(s) 1637
SsiI CCGC 8 cut(s) 6, 9, 147, 1173, 1644, 1673, 1790, 2162
SspMI CTAG 4 cut(s) 21, 489, 1224, 1472
StyD4I CCNGG 7 cut(s) 253, 394, 1479, 1882, 2011, 2113, 2154
StyI CCWWGG 1 cut(s) 289
TaaI ACNGT 8 cut(s) 985, 1148, 1235, 1380, 1830, 1996, 2151, 2240
TaiI ACGT 2 cut(s) 199, 801
TaqI TCGA 5 cut(s) 242, 944, 1022, 1535, 1946
TatI WGTACW 2 cut(s) 43, 2034
TauI GCSGC 2 cut(s) 9, 2165
Tru1I TTAA 8 cut(s) 468, 596, 993, 1082, 1110, 1143, 1706, 1776
Tru9I TTAA 8 cut(s) 468, 596, 993, 1082, 1110, 1143, 1706, 1776
TscAI CASTG 4 cut(s) 1240, 1281, 1429, 1927
TseFI GTSAC 1 cut(s) 821
TseI GCWGC 9 cut(s) 332, 422, 1604, 1763, 1766, 2159, 2171, 2198, 2209
Tsp45I GTSAC 1 cut(s) 821
TspDTI ATGAA 7 cut(s) 114, 167, 1215, 1842, 1846, 1994, 2243
TspRI CASTG 4 cut(s) 1240, 1281, 1429, 1927
VpaK11BI GGWCC 2 cut(s) 1069, 2186
VspI ATTAAT 1 cut(s) 1110
XagI CCTNNNNNAGG 1 cut(s) 2045
XapI RAATTY 3 cut(s) 325, 643, 1549
XceI RCATGY 2 cut(s) 1637, 2222
XcmI CCANNNNNNNNNTGG 2 cut(s) 2084, 2162
XhoI CTCGAG 1 cut(s) 1021
XmiI GTMKAC 1 cut(s) 944
XmnI GAANNNNTTC 1 cut(s) 1652
XspI CTAG 4 cut(s) 21, 489, 1224, 1472
Zsp2I ATGCAT 1 cut(s) 1639
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.