RLG00000014897

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000144
Physical Location & Seq
Reverse (-)
59251763 .. 59253544
1782 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014897

Sequence Viewer

Length: 1782 bp
ATGATGATCGAGTCTCTATTCGCTTGCTTCAATCCTCGATGCAATACGAGCAAGCTTAGGCTGCTAAAGAAAAAAAAACTCCCAAAGAAATTAGAGAGAGAGCAGAAGGAGAATAAAGATGATAAGTACAGAAACCAAACCAGTTCTAATGAATGTACCTTTTGGCAGCCACCACCTCCTGATATCATGGAGCAAGTTCTGCCCCGTGTCAGAGTAAGTGATCAAATAAGGCTAAGCATTGCCTGCAAGTCTTGGATGTCAATTGTTATGCGTAAAGACATCCGCAGTGCTTCCCATGAATTCCCATGGTTACTACTCCCTCAAACCCCACATTGCAGCAACAAGTACGTAAGCTTTGCCAGCGTGTCAGAGGGGAGAGTTGTCAAGTTGAAGCTACCTAAGCAAGTTCGTGGATGGTGGATTTATGGGTCTTCTAAAGGTTGGTTGATCATGATCAAGGAAAGAGGTCTAAATTCCAAGATGTGTCTACTTAACCCAATTTCAGGAGCCCTACTCCGACTTCCACCCTTGAGAACACTTCCTTTATTGAAAGATTTTATGAAAACCGAGGACTGGAAACTTTTTGGTGCCAATGCATTCGACTTGAGGATTGCACTATCAACCTCTGATGGAATCGCTTTAGATTCAAAGCGTTGTACGGTAGCAGCAGTTTTTAATGATAAGTCGAAATTGAGTTTGTGCAGACCTGGAGACAGAACATGGAATTACTTTCAAGTATTAGATACTAATCAAAACGACTGGATTGCTGATTTATTGTTTTCTTCCGGCAGTCTATATGTTTTGGTTCGTAGTAGTCAAGAGGAAAGCTTTGTAGACTCAGTTACTCAAACCTTGAACTTTGGCTTTGGAGATGCAGAAAATTTGAAAATGAAGTTGGTCTACGACAAGCGTGAAAACAGGAACCTGAACGTTAATGAATGTCATAGTGACTATAAGATCGTTTACAATGCATTATACTTCTCAAGATTGTTAGAATCAACCAGCAATGAAGTCTTATTGATCCATCAAATGGTAGATTATGTTGTGGGAAGAGGAGAGGCTATTATTGGTGACAATAATGAGAATAATGGCGGCGGCAATATGGAAAATGATGACGGTGAGGAACACATTCATCATGATAATGACGACGGAAATCTTGAGGTTGATGGGGTAGACATCGATCAGAATGACGAGGATGAGGATGAAGCCAACAACTTAGAAGATATTGATCAGCAAAATGGAGGAAACATTATTTATGGTGATGAAGGCAGTAGTGAGAATGATGGTGGCGACAGCAATATGGAAAATGATGATGGTGGTGAAGGCCATAACGACAATAATGATGTTGATGTGGGAGACATTAATCAGAATCCTGAAGGTGATGAGGAAGACAACAATAACCCAGAAGATAATGGTCAGCAAGGAGAAACTATTGATGAAGATGCACCGGGATTTAATGCATATATGACAACAAGGAGTTTTAGAGTATACAAGACTGACGACGACAACTTTATTCCATTGCAATGCTTGGGGGATCAATTATTCTTTCTTGGAGATTGTGGTTCCTTCTCCTTTGGAGTCAGTAATATGAAAAAATCAGAAAGGAATTGTATTTATTTTGCATCAAATTTGATGTACGATTGGGAGGCAGCTCCAAAAACGTATACATCTCGTGACATTGGCATATTCTACCTAGATAGCCAAAGAATTGAGCGGTCATTTCCAAGTGTTGAGATGTCTCTACGGTATCAAGGGACTTGGTTCACTCCAAGTTTATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

594

Amino Acids

67.54

Weight (kDa)

4.67

Isoelectric Point (pI)

41.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 119 - 343 3.3e-20 KIB1-4 beta-propeller
Beta-prop_KIB1-4 PF03478 488 - 563 8.9e-09 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 587
AccB7I CCANNNNNTGG 1 cut(s) 1030
AccBSI CCGCTC 1 cut(s) 1714
AccI GTMKAC 6 cut(s) 487, 834, 900, 1173, 1488, 1664
AciI CCGC 4 cut(s) 283, 1092, 1095, 1714
AclI AACGTT 1 cut(s) 930
AclWI GGATC 2 cut(s) 1015, 1542
AcsI RAATTY 4 cut(s) 299, 472, 880, 1627
AcuI CTGAAG 1 cut(s) 1395
AfaI GTAC 5 cut(s) 128, 157, 347, 658, 1637
AfiI CCNNNNNNNGG 3 cut(s) 503, 573, 1030
AgsI TTSAA 7 cut(s) 31, 391, 550, 648, 734, 856, 886
AjnI CCWGG 1 cut(s) 706
AjuI GAANNNNNNNTTGG 2 cut(s) 878, 910
AluBI AGCT 5 cut(s) 55, 354, 394, 828, 1652
AluI AGCT 5 cut(s) 55, 354, 394, 828, 1652
Alw26I GTCTC 4 cut(s) 18, 705, 1350, 1743
AlwI GGATC 2 cut(s) 1015, 1542
AoxI GGCC 1 cut(s) 1324
ApeKI GCWGC 5 cut(s) 61, 166, 336, 665, 1649
ApoI RAATTY 4 cut(s) 299, 472, 880, 1627
AseI ATTAAT 1 cut(s) 1362
Asp700I GAANNNNTTC 1 cut(s) 1128
AsuC2I CCSGG 1 cut(s) 1449
AsuHPI GGTGA 5 cut(s) 1082, 1130, 1271, 1331, 1391
BanI GGYRCC 1 cut(s) 587
BanII GRGCYC 1 cut(s) 511
BarI GAAGNNNNNNTAC 2 cut(s) 884, 916
BauI CACGAG 1 cut(s) 1671
BbsI GAAGAC 2 cut(s) 423, 1395
BbvI GCAGC 5 cut(s) 48, 178, 348, 677, 1661
BccI CCATC 6 cut(s) 408, 623, 1032, 1160, 1277, 1307
BcgI CGANNNNNNTGC 2 cut(s) 746, 780
BciT130I CCWGG 1 cut(s) 708
BclI TGATCA 4 cut(s) 220, 447, 453, 1228
BcnI CCSGG 1 cut(s) 1449
BcoDI GTCTC 4 cut(s) 18, 705, 1350, 1743
BfaI CTAG 1 cut(s) 1694
BisI GCNGC 7 cut(s) 62, 167, 337, 666, 1093, 1096, 1650
BlpI GCTNAGC 1 cut(s) 233
BlsI GCNGC 7 cut(s) 63, 168, 338, 667, 1094, 1097, 1651
Bme1390I CCNGG 2 cut(s) 708, 1449
BmiI GGNNCC 4 cut(s) 508, 589, 923, 1564
BmrFI CCNGG 2 cut(s) 708, 1449
BmsI GCATC 4 cut(s) 29, 862, 1432, 1631
BpiI GAAGAC 2 cut(s) 423, 1395
BplI GAGNNNNNCTC 2 cut(s) 498, 530
BpmI CTGGAG 1 cut(s) 729
Bpu10I CCTNAGC 2 cut(s) 56, 399
Bpu1102I GCTNAGC 1 cut(s) 233
BpuEI CTTGAG 4 cut(s) 550, 625, 967, 1178
BpuMI CCSGG 1 cut(s) 1449
Bsa29I ATCGAT 1 cut(s) 1179
BsaAI YACGTR 1 cut(s) 349
BsaBI GATNNNNATC 3 cut(s) 452, 747, 1227
BsaJI CCNNGG 2 cut(s) 305, 567
Bsc4I CCNNNNNNNGG 3 cut(s) 503, 573, 1030
Bse1I ACTGG 3 cut(s) 141, 578, 764
Bse3DI GCAATG 5 cut(s) 237, 331, 1012, 1517, 1529
Bse8I GATNNNNATC 3 cut(s) 452, 747, 1227
BseBI CCWGG 1 cut(s) 708
BseCI ATCGAT 1 cut(s) 1179
BseDI CCNNGG 2 cut(s) 305, 567
BseGI GGATG 5 cut(s) 261, 279, 419, 1201, 1207
BseJI GATNNNNATC 3 cut(s) 452, 747, 1227
BseLI CCNNNNNNNGG 3 cut(s) 503, 573, 1030
BseMI GCAATG 5 cut(s) 237, 331, 1012, 1517, 1529
BseMII CTCAG 1 cut(s) 852
BseNI ACTGG 3 cut(s) 141, 578, 764
BseRI GAGGAG 1 cut(s) 1068
BseXI GCAGC 5 cut(s) 48, 178, 348, 677, 1661
BsgI GTGCAG 1 cut(s) 721
BshFI GGCC 1 cut(s) 1326
BshNI GGYRCC 1 cut(s) 587
BshVI ATCGAT 1 cut(s) 1179
BsiSI CCGG 2 cut(s) 786, 1448
BslFI GGGAC 1 cut(s) 1768
BslI CCNNNNNNNGG 3 cut(s) 503, 573, 1030
BsmAI GTCTC 4 cut(s) 18, 705, 1350, 1743
BsmFI GGGAC 1 cut(s) 1768
BsmI GAATGC 1 cut(s) 596
BsnI GGCC 1 cut(s) 1326
Bsp1286I GDGCHC 1 cut(s) 511
Bsp143I GATC 9 cut(s) 6, 220, 447, 453, 957, 1020, 1180, 1228, 1534
Bsp1720I GCTNAGC 1 cut(s) 233
Bsp19I CCATGG 1 cut(s) 305
BspACI CCGC 4 cut(s) 283, 1092, 1095, 1714
BspANI GGCC 1 cut(s) 1326
BspCNI CTCAG 1 cut(s) 851
BspDI ATCGAT 1 cut(s) 1179
BspHI TCATGA 2 cut(s) 450, 1135
BspLI GGNNCC 4 cut(s) 508, 589, 923, 1564
BspPI GGATC 2 cut(s) 1015, 1542
BspT107I GGYRCC 1 cut(s) 587
BsrBI CCGCTC 1 cut(s) 1714
BsrDI GCAATG 5 cut(s) 237, 331, 1012, 1517, 1529
BsrI ACTGG 3 cut(s) 141, 578, 764
BssECI CCNNGG 2 cut(s) 305, 567
BssMI GATC 9 cut(s) 6, 220, 447, 453, 957, 1020, 1180, 1228, 1534
BssNAI GTATAC 2 cut(s) 1489, 1665
BssSI CACGAG 1 cut(s) 1671
BssT1I CCWWGG 1 cut(s) 305
Bst1107I GTATAC 2 cut(s) 1489, 1665
Bst2BI CACGAG 1 cut(s) 1671
Bst2UI CCWGG 1 cut(s) 708
Bst4CI ACNGT 3 cut(s) 661, 1118, 1746
Bst6I CTCTTC 1 cut(s) 1045
BstAPI GCANNNNNTGC 2 cut(s) 199, 243
BstBAI YACGTR 1 cut(s) 349
BstC8I GCNNGC 4 cut(s) 25, 53, 244, 361
BstDEI CTNAG 5 cut(s) 56, 233, 399, 838, 1216
BstDSI CCRYGG 1 cut(s) 305
BstF5I GGATG 5 cut(s) 261, 279, 419, 1201, 1207
BstKTI GATC 9 cut(s) 9, 223, 450, 456, 960, 1023, 1183, 1231, 1537
BstMAI GTCTC 4 cut(s) 18, 705, 1350, 1743
BstMBI GATC 9 cut(s) 6, 220, 447, 453, 957, 1020, 1180, 1228, 1534
BstMWI GCNNNNNNNGC 6 cut(s) 48, 61, 199, 243, 360, 400
BstNI CCWGG 1 cut(s) 708
BstSCI CCNGG 2 cut(s) 706, 1447
BstSNI TACGTA 1 cut(s) 349
BstV1I GCAGC 5 cut(s) 48, 178, 348, 677, 1661
BstV2I GAAGAC 2 cut(s) 423, 1395
BstZ17I GTATAC 2 cut(s) 1489, 1665
Bsu15I ATCGAT 1 cut(s) 1179
BsuRI GGCC 1 cut(s) 1326
BsuTUI ATCGAT 1 cut(s) 1179
BtgI CCRYGG 1 cut(s) 305
BtsCI GGATG 5 cut(s) 261, 279, 419, 1201, 1207
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 1 cut(s) 292
Cac8I GCNNGC 4 cut(s) 25, 53, 244, 361
CciI TCATGA 2 cut(s) 450, 1135
ClaI ATCGAT 1 cut(s) 1179
Csp6I GTAC 5 cut(s) 127, 156, 346, 657, 1636
CviAII CATG 6 cut(s) 187, 296, 306, 451, 720, 1136
CviQI GTAC 5 cut(s) 127, 156, 346, 657, 1636
DdeI CTNAG 5 cut(s) 56, 233, 399, 838, 1216
DpnI GATC 9 cut(s) 8, 222, 449, 455, 959, 1022, 1182, 1230, 1536
DpnII GATC 9 cut(s) 6, 220, 447, 453, 957, 1020, 1180, 1228, 1534
Eam1104I CTCTTC 1 cut(s) 1045
EarI CTCTTC 1 cut(s) 1045
Eco105I TACGTA 1 cut(s) 349
Eco130I CCWWGG 1 cut(s) 305
Eco24I GRGCYC 1 cut(s) 511
Eco32I GATATC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 1395
EcoRI GAATTC 1 cut(s) 299
EcoRII CCWGG 1 cut(s) 706
EcoRV GATATC 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 305
EcoT22I ATGCAT 3 cut(s) 598, 973, 1462
EcoT38I GRGCYC 1 cut(s) 511
ErhI CCWWGG 1 cut(s) 305
FaeI CATG 6 cut(s) 190, 299, 309, 454, 723, 1139
FaqI GGGAC 1 cut(s) 1768
FatI CATG 6 cut(s) 186, 295, 305, 450, 719, 1135
FbaI TGATCA 4 cut(s) 220, 447, 453, 1228
FblI GTMKAC 6 cut(s) 487, 834, 900, 1173, 1488, 1664
Fnu4HI GCNGC 7 cut(s) 62, 167, 337, 666, 1093, 1096, 1650
FokI GGATG 5 cut(s) 266, 268, 426, 1208, 1214
FriOI GRGCYC 1 cut(s) 511
Fsp4HI GCNGC 7 cut(s) 62, 167, 337, 666, 1093, 1096, 1650
FspBI CTAG 1 cut(s) 1694
GluI GCNGC 7 cut(s) 62, 167, 337, 666, 1093, 1096, 1650
GsuI CTGGAG 1 cut(s) 729
HaeIII GGCC 1 cut(s) 1326
HapII CCGG 2 cut(s) 786, 1448
Hin1II CATG 6 cut(s) 190, 299, 309, 454, 723, 1139
HindIII AAGCTT 3 cut(s) 53, 352, 826
HinfI GANTC 7 cut(s) 11, 633, 644, 836, 995, 1369, 1578
HpaII CCGG 2 cut(s) 786, 1448
HphI GGTGA 5 cut(s) 1082, 1130, 1271, 1331, 1391
Hpy166II GTNNAC 8 cut(s) 488, 835, 901, 964, 1174, 1489, 1665, 1764
Hpy188I TCNGA 7 cut(s) 212, 370, 518, 628, 1185, 1368, 1600
Hpy188III TCNNGA 9 cut(s) 179, 451, 504, 818, 984, 1136, 1157, 1373, 1673
Hpy8I GTNNAC 8 cut(s) 488, 835, 901, 964, 1174, 1489, 1665, 1764
Hpy99I CGWCG 2 cut(s) 1151, 1505
HpyAV CCTTC 5 cut(s) 100, 1259, 1316, 1370, 1576
HpyCH4III ACNGT 3 cut(s) 661, 1118, 1746
HpyCH4IV ACGT 3 cut(s) 348, 930, 1661
HpyF10VI GCNNNNNNNGC 6 cut(s) 48, 61, 199, 243, 360, 400
HpyF3I CTNAG 5 cut(s) 56, 233, 399, 838, 1216
HpySE526I ACGT 3 cut(s) 348, 930, 1661
Hsp92II CATG 6 cut(s) 190, 299, 309, 454, 723, 1139
Ksp22I TGATCA 4 cut(s) 220, 447, 453, 1228
Kzo9I GATC 9 cut(s) 6, 220, 447, 453, 957, 1020, 1180, 1228, 1534
LmnI GCTCC 3 cut(s) 190, 506, 1657
Lsp1109I GCAGC 5 cut(s) 48, 178, 348, 677, 1661
LweI GCATC 4 cut(s) 29, 862, 1432, 1631
MaeI CTAG 1 cut(s) 1694
MaeII ACGT 3 cut(s) 348, 930, 1661
MaeIII GTNAC 5 cut(s) 309, 841, 947, 1070, 1673
MalI GATC 9 cut(s) 8, 222, 449, 455, 959, 1022, 1182, 1230, 1536
MbiI CCGCTC 1 cut(s) 1714
MboI GATC 9 cut(s) 6, 220, 447, 453, 957, 1020, 1180, 1228, 1534
MboII GAAGA 7 cut(s) 423, 774, 1062, 1232, 1400, 1418, 1451
MfeI CAATTG 1 cut(s) 261
MhlI GDGCHC 1 cut(s) 511
MlyI GAGTC 3 cut(s) 20, 830, 1587
MmeI TCCRAC 1 cut(s) 541
Mph1103I ATGCAT 3 cut(s) 598, 973, 1462
MroXI GAANNNNTTC 1 cut(s) 1128
MseI TTAA 5 cut(s) 492, 675, 933, 1362, 1455
MslI CAYNNNNRTG 2 cut(s) 1140, 1522
MspI CCGG 2 cut(s) 786, 1448
MspR9I CCNGG 2 cut(s) 708, 1449
MunI CAATTG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 596
MvaI CCWGG 1 cut(s) 708
MwoI GCNNNNNNNGC 6 cut(s) 48, 61, 199, 243, 360, 400
NciI CCSGG 1 cut(s) 1449
NcoI CCATGG 1 cut(s) 305
NdeII GATC 9 cut(s) 6, 220, 447, 453, 957, 1020, 1180, 1228, 1534
NlaIII CATG 6 cut(s) 190, 299, 309, 454, 723, 1139
NlaIV GGNNCC 4 cut(s) 508, 589, 923, 1564
NmuCI GTSAC 3 cut(s) 947, 1070, 1673
NsiI ATGCAT 3 cut(s) 598, 973, 1462
PagI TCATGA 2 cut(s) 450, 1135
PctI GAATGC 1 cut(s) 596
PdmI GAANNNNTTC 1 cut(s) 1128
PfeI GAWTC 4 cut(s) 633, 644, 995, 1369
PflMI CCANNNNNTGG 1 cut(s) 1030
PkrI GCNGC 7 cut(s) 63, 168, 338, 667, 1094, 1097, 1651
PleI GAGTC 3 cut(s) 19, 830, 1586
PpsI GAGTC 3 cut(s) 19, 830, 1586
Ppu21I YACGTR 1 cut(s) 349
PshBI ATTAAT 1 cut(s) 1362
Psp1406I AACGTT 1 cut(s) 930
Psp6I CCWGG 1 cut(s) 706
PspGI CCWGG 1 cut(s) 706
PspN4I GGNNCC 4 cut(s) 508, 589, 923, 1564
RsaI GTAC 5 cut(s) 128, 157, 347, 658, 1637
RsaNI GTAC 5 cut(s) 127, 156, 346, 657, 1636
RseI CAYNNNNRTG 2 cut(s) 1140, 1522
SaqAI TTAA 5 cut(s) 492, 675, 933, 1362, 1455
SatI GCNGC 7 cut(s) 62, 167, 337, 666, 1093, 1096, 1650
Sau3AI GATC 9 cut(s) 6, 220, 447, 453, 957, 1020, 1180, 1228, 1534
SchI GAGTC 3 cut(s) 20, 830, 1587
ScrFI CCNGG 2 cut(s) 708, 1449
SduI GDGCHC 1 cut(s) 511
SfaNI GCATC 4 cut(s) 29, 862, 1432, 1631
SmiMI CAYNNNNRTG 2 cut(s) 1140, 1522
SmlI CTYRAG 4 cut(s) 529, 604, 982, 1157
SmoI CTYRAG 4 cut(s) 529, 604, 982, 1157
SnaBI TACGTA 1 cut(s) 349
SsiI CCGC 4 cut(s) 283, 1092, 1095, 1714
SspMI CTAG 1 cut(s) 1694
StyD4I CCNGG 2 cut(s) 706, 1447
StyI CCWWGG 1 cut(s) 305
TaaI ACNGT 3 cut(s) 661, 1118, 1746
TaiI ACGT 3 cut(s) 351, 933, 1664
TaqI TCGA 5 cut(s) 9, 37, 600, 686, 1179
TatI WGTACW 1 cut(s) 126
TauI GCSGC 2 cut(s) 1095, 1098
TfiI GAWTC 4 cut(s) 633, 644, 995, 1369
Tru1I TTAA 5 cut(s) 492, 675, 933, 1362, 1455
Tru9I TTAA 5 cut(s) 492, 675, 933, 1362, 1455
TscAI CASTG 1 cut(s) 292
TseFI GTSAC 3 cut(s) 947, 1070, 1673
TseI GCWGC 5 cut(s) 61, 166, 336, 665, 1649
Tsp45I GTSAC 3 cut(s) 947, 1070, 1673
TspGWI ACGGA 1 cut(s) 1164
TspRI CASTG 1 cut(s) 292
Van91I CCANNNNNTGG 1 cut(s) 1030
VspI ATTAAT 1 cut(s) 1362
XapI RAATTY 4 cut(s) 299, 472, 880, 1627
XmiI GTMKAC 6 cut(s) 487, 834, 900, 1173, 1488, 1664
XmnI GAANNNNTTC 1 cut(s) 1128
XspI CTAG 1 cut(s) 1694
Zsp2I ATGCAT 3 cut(s) 598, 973, 1462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.