Rh6AG062600

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000144
Physical Location & Seq
Reverse (-)
9324221 .. 9324943
723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG062600.1

Sequence Viewer

Length: 723 bp
ATGCGCAGTGCCCGTCGTCAACTACCATGGTTAATACTCCCTGAAGCCCCAAATTGCAGGATCAACAAGTACTTGAGCTTTCTCACCTTCCCTGAGAATAAACTCTCTAAGGGCAAAGCTGTTAAGTTAAAGCTGCCTAAGCAACTTCAAGGAGGGTGGATATACGGGTCTTCTAAAGCTTGGTTGATCATCATCAAGGAAAAGGGACTAAACTCTGAGATGTGTCTACTAAACCCAATTTCAGGAGTCCTACACAAACTTCCGCCCTTGAGAACAATTCCATCTTTCAAAAACTTTGTAAAAACCAGGGAATGGGAACTTCTGGGTGCCGAAGGATTCTGTAATTGCGATAAAATGTCTACCCGGGATGGTTATAATTCTTCAAGCAACTTTACTGTGGCTGCAATTTTCAATGATCGGAATACTTTATGTTTGTGTAGACCAGGATATGAAAAATGGAGTGTCTTCCCGGAATTAGACTACCTCATTAATATGTTGTTTTCTTCTGGCACGCTATATGCTTTAGTCTGGAGTGAACAAAATGACGGCGGCGTCGCACCTACTTGCCCCTTAAACTTTCCATATCATGATGCTGTACACTTGAAGTTGAAGTTGGCCTATGACAAACACGAAAGCAGGAATTACATCATTGATGACTATCATGGTAACTATAAGATAGCTTCTAATGCACACACGTCAATGTTGTTAATTAGAATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

240

Amino Acids

27.42

Weight (kDa)

9.25

Isoelectric Point (pI)

40.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 35 - 178 1.4e-15 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AasI GACNNNNNNGTC 1 cut(s) 551
Acc16I TGCGCA 1 cut(s) 5
AccB1I GGYRCC 1 cut(s) 326
AccB7I CCANNNNNTGG 1 cut(s) 312
AccI GTMKAC 3 cut(s) 226, 359, 439
AciI CCGC 2 cut(s) 263, 549
AclWI GGATC 1 cut(s) 68
AcuI CTGAAG 1 cut(s) 63
AcyI GRCGYC 1 cut(s) 552
AfaI GTAC 2 cut(s) 71, 597
AfiI CCNNNNNNNGG 2 cut(s) 242, 312
AflIII ACRYGT 1 cut(s) 693
AgsI TTSAA 6 cut(s) 149, 289, 384, 412, 604, 610
AjiI CACGTC 1 cut(s) 696
AjnI CCWGG 2 cut(s) 305, 442
AjuI GAANNNNNNNTTGG 2 cut(s) 596, 628
AluBI AGCT 5 cut(s) 78, 119, 133, 179, 680
AluI AGCT 5 cut(s) 78, 119, 133, 179, 680
AlwI GGATC 1 cut(s) 68
Ama87I CYCGRG 1 cut(s) 363
AoxI GGCC 1 cut(s) 615
ApeKI GCWGC 2 cut(s) 133, 401
AseI ATTAAT 1 cut(s) 489
AspLEI GCGC 1 cut(s) 6
AsuC2I CCSGG 3 cut(s) 364, 365, 470
AsuHPI GGTGA 1 cut(s) 76
AvaI CYCGRG 1 cut(s) 363
BaeGI GKGCMC 1 cut(s) 13
BanI GGYRCC 1 cut(s) 326
BbsI GAAGAC 2 cut(s) 162, 457
BbvI GCAGC 2 cut(s) 120, 388
BccI CCATC 2 cut(s) 289, 362
BceAI ACGGC 1 cut(s) 562
BciT130I CCWGG 2 cut(s) 307, 444
BclI TGATCA 1 cut(s) 186
BcnI CCSGG 3 cut(s) 364, 365, 470
BfaI CTAG 1 cut(s) 721
BisI GCNGC 3 cut(s) 134, 402, 550
BlsI GCNGC 3 cut(s) 135, 403, 551
BmcAI AGTACT 1 cut(s) 71
Bme1390I CCNGG 5 cut(s) 307, 364, 365, 444, 470
BmeT110I CYCGRG 1 cut(s) 363
BmgBI CACGTC 1 cut(s) 696
BmiI GGNNCC 1 cut(s) 328
BmrFI CCNGG 5 cut(s) 307, 364, 365, 444, 470
BmsI GCATC 1 cut(s) 580
BpiI GAAGAC 2 cut(s) 162, 457
BpmI CTGGAG 1 cut(s) 550
Bpu10I CCTNAGC 1 cut(s) 138
BpuEI CTTGAG 2 cut(s) 94, 289
BpuMI CCSGG 3 cut(s) 364, 365, 470
BsaBI GATNNNNATC 2 cut(s) 191, 657
BsaHI GRCGYC 1 cut(s) 552
BsaJI CCNNGG 3 cut(s) 26, 306, 363
Bsc4I CCNNNNNNNGG 2 cut(s) 242, 312
Bse8I GATNNNNATC 2 cut(s) 191, 657
BseBI CCWGG 2 cut(s) 307, 444
BseDI CCNNGG 3 cut(s) 26, 306, 363
BseGI GGATG 1 cut(s) 373
BseJI GATNNNNATC 2 cut(s) 191, 657
BseLI CCNNNNNNNGG 2 cut(s) 242, 312
BseMII CTCAG 2 cut(s) 84, 207
BseSI GKGCMC 1 cut(s) 13
BseXI GCAGC 2 cut(s) 120, 388
BshFI GGCC 1 cut(s) 617
BshNI GGYRCC 1 cut(s) 326
BsiHKCI CYCGRG 1 cut(s) 363
BsiSI CCGG 2 cut(s) 364, 470
BslFI GGGAC 1 cut(s) 219
BslI CCNNNNNNNGG 2 cut(s) 242, 312
BsmFI GGGAC 1 cut(s) 219
BsnI GGCC 1 cut(s) 617
BsoBI CYCGRG 1 cut(s) 363
Bsp1286I GDGCHC 1 cut(s) 13
Bsp1407I TGTACA 1 cut(s) 595
Bsp143I GATC 3 cut(s) 60, 186, 415
Bsp19I CCATGG 1 cut(s) 26
BspACI CCGC 2 cut(s) 263, 549
BspANI GGCC 1 cut(s) 617
BspCNI CTCAG 2 cut(s) 85, 208
BspHI TCATGA 1 cut(s) 586
BspLI GGNNCC 1 cut(s) 328
BspPI GGATC 1 cut(s) 68
BspT107I GGYRCC 1 cut(s) 326
BsrGI TGTACA 1 cut(s) 595
BssECI CCNNGG 3 cut(s) 26, 306, 363
BssMI GATC 3 cut(s) 60, 186, 415
BssNI GRCGYC 1 cut(s) 552
BssT1I CCWWGG 1 cut(s) 26
Bst2UI CCWGG 2 cut(s) 307, 444
Bst4CI ACNGT 1 cut(s) 397
BstACI GRCGYC 1 cut(s) 552
BstAUI TGTACA 1 cut(s) 595
BstC8I GCNNGC 1 cut(s) 512
BstDEI CTNAG 4 cut(s) 93, 108, 138, 216
BstDSI CCRYGG 1 cut(s) 26
BstF5I GGATG 1 cut(s) 373
BstHHI GCGC 1 cut(s) 6
BstKTI GATC 3 cut(s) 63, 189, 418
BstMBI GATC 3 cut(s) 60, 186, 415
BstMWI GCNNNNNNNGC 2 cut(s) 139, 686
BstNI CCWGG 2 cut(s) 307, 444
BstSCI CCNGG 5 cut(s) 305, 362, 363, 442, 468
BstSLI GKGCMC 1 cut(s) 13
BstV1I GCAGC 2 cut(s) 120, 388
BstV2I GAAGAC 2 cut(s) 162, 457
BsuRI GGCC 1 cut(s) 617
BtgI CCRYGG 1 cut(s) 26
BtrI CACGTC 1 cut(s) 696
BtsCI GGATG 1 cut(s) 373
BtsI GCAGTG 1 cut(s) 13
BtsIMutI CAGTG 1 cut(s) 13
Cac8I GCNNGC 1 cut(s) 512
CciI TCATGA 1 cut(s) 586
CfoI GCGC 1 cut(s) 6
Cfr9I CCCGGG 1 cut(s) 363
CseI GACGC 1 cut(s) 541
Csp6I GTAC 2 cut(s) 70, 596
CspCI CAANNNNNGTGG 2 cut(s) 137, 172
CviAII CATG 3 cut(s) 27, 587, 662
CviJI RGCY 8 cut(s) 47, 78, 119, 133, 179, 401, 617, 680
CviKI_1 RGCY 8 cut(s) 47, 78, 119, 133, 179, 401, 617, 680
CviQI GTAC 2 cut(s) 70, 596
DdeI CTNAG 4 cut(s) 93, 108, 138, 216
DpnI GATC 3 cut(s) 62, 188, 417
DpnII GATC 3 cut(s) 60, 186, 415
DrdI GACNNNNNNGTC 1 cut(s) 551
DseDI GACNNNNNNGTC 1 cut(s) 551
EciI GGCGGA 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 26
Eco57I CTGAAG 1 cut(s) 63
Eco88I CYCGRG 1 cut(s) 363
EcoRII CCWGG 2 cut(s) 305, 442
EcoT14I CCWWGG 1 cut(s) 26
ErhI CCWWGG 1 cut(s) 26
FaeI CATG 3 cut(s) 30, 590, 665
FaqI GGGAC 1 cut(s) 219
FatI CATG 3 cut(s) 26, 586, 661
FbaI TGATCA 1 cut(s) 186
FblI GTMKAC 3 cut(s) 226, 359, 439
Fnu4HI GCNGC 3 cut(s) 134, 402, 550
FokI GGATG 1 cut(s) 380
Fsp4HI GCNGC 3 cut(s) 134, 402, 550
FspBI CTAG 1 cut(s) 721
FspI TGCGCA 1 cut(s) 5
GlaI GCGC 1 cut(s) 5
GluI GCNGC 3 cut(s) 134, 402, 550
GsuI CTGGAG 1 cut(s) 550
HaeIII GGCC 1 cut(s) 617
HapII CCGG 2 cut(s) 364, 470
HgaI GACGC 1 cut(s) 541
HhaI GCGC 1 cut(s) 6
Hin1I GRCGYC 1 cut(s) 552
Hin1II CATG 3 cut(s) 30, 590, 665
Hin6I GCGC 1 cut(s) 4
HinP1I GCGC 1 cut(s) 4
HincII GTYRAC 1 cut(s) 20
HindII GTYRAC 1 cut(s) 20
HindIII AAGCTT 1 cut(s) 177
HinfI GANTC 3 cut(s) 246, 336, 714
HpaII CCGG 2 cut(s) 364, 470
HphI GGTGA 1 cut(s) 76
Hpy166II GTNNAC 6 cut(s) 20, 227, 360, 440, 536, 598
Hpy188I TCNGA 2 cut(s) 217, 420
Hpy188III TCNNGA 3 cut(s) 243, 529, 587
Hpy8I GTNNAC 6 cut(s) 20, 227, 360, 440, 536, 598
Hpy99I CGWCG 2 cut(s) 18, 557
HpyAV CCTTC 2 cut(s) 97, 326
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 1 cut(s) 695
HpyCH4V TGCA 3 cut(s) 57, 404, 689
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 686
HpyF3I CTNAG 4 cut(s) 93, 108, 138, 216
HpySE526I ACGT 1 cut(s) 695
Hsp92I GRCGYC 1 cut(s) 552
Hsp92II CATG 3 cut(s) 30, 590, 665
HspAI GCGC 1 cut(s) 4
Ksp22I TGATCA 1 cut(s) 186
Kzo9I GATC 3 cut(s) 60, 186, 415
Lsp1109I GCAGC 2 cut(s) 120, 388
LweI GCATC 1 cut(s) 580
MaeI CTAG 1 cut(s) 721
MaeII ACGT 1 cut(s) 695
MaeIII GTNAC 1 cut(s) 665
MalI GATC 3 cut(s) 62, 188, 417
MboI GATC 3 cut(s) 60, 186, 415
MboII GAAGA 4 cut(s) 162, 372, 457, 495
MhlI GDGCHC 1 cut(s) 13
MluCI AATT 9 cut(s) 52, 237, 276, 343, 376, 405, 473, 640, 708
MlyI GAGTC 1 cut(s) 255
MnlI CCTC 2 cut(s) 146, 494
MseI TTAA 6 cut(s) 32, 123, 128, 489, 572, 707
MslI CAYNNNNRTG 2 cut(s) 491, 698
MspI CCGG 2 cut(s) 364, 470
MspR9I CCNGG 5 cut(s) 307, 364, 365, 444, 470
MvaI CCWGG 2 cut(s) 307, 444
MwoI GCNNNNNNNGC 2 cut(s) 139, 686
NciI CCSGG 3 cut(s) 364, 365, 470
NcoI CCATGG 1 cut(s) 26
NdeII GATC 3 cut(s) 60, 186, 415
NlaIII CATG 3 cut(s) 30, 590, 665
NlaIV GGNNCC 1 cut(s) 328
NsbI TGCGCA 1 cut(s) 5
PagI TCATGA 1 cut(s) 586
PfeI GAWTC 2 cut(s) 336, 714
PflMI CCANNNNNTGG 1 cut(s) 312
PfoI TCCNGGA 1 cut(s) 468
PkrI GCNGC 3 cut(s) 135, 403, 551
PleI GAGTC 1 cut(s) 254
PpsI GAGTC 1 cut(s) 254
PshBI ATTAAT 1 cut(s) 489
PsiI TTATAA 1 cut(s) 375
Psp6I CCWGG 2 cut(s) 305, 442
PspGI CCWGG 2 cut(s) 305, 442
PspN4I GGNNCC 1 cut(s) 328
RsaI GTAC 2 cut(s) 71, 597
RsaNI GTAC 2 cut(s) 70, 596
RseI CAYNNNNRTG 2 cut(s) 491, 698
SaqAI TTAA 6 cut(s) 32, 123, 128, 489, 572, 707
SatI GCNGC 3 cut(s) 134, 402, 550
Sau3AI GATC 3 cut(s) 60, 186, 415
ScaI AGTACT 1 cut(s) 71
SchI GAGTC 1 cut(s) 255
ScrFI CCNGG 5 cut(s) 307, 364, 365, 444, 470
SduI GDGCHC 1 cut(s) 13
SetI ASST 9 cut(s) 80, 89, 121, 135, 181, 486, 562, 682, 698
SfaNI GCATC 1 cut(s) 580
SmaI CCCGGG 1 cut(s) 365
SmiMI CAYNNNNRTG 2 cut(s) 491, 698
SmlI CTYRAG 2 cut(s) 73, 268
SmoI CTYRAG 2 cut(s) 73, 268
Sse9I AATT 9 cut(s) 52, 237, 276, 343, 376, 405, 473, 640, 708
SsiI CCGC 2 cut(s) 263, 549
SspMI CTAG 1 cut(s) 721
StyD4I CCNGG 5 cut(s) 305, 362, 363, 442, 468
StyI CCWWGG 1 cut(s) 26
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 1 cut(s) 698
TasI AATT 9 cut(s) 52, 237, 276, 343, 376, 405, 473, 640, 708
TatI WGTACW 2 cut(s) 69, 595
TauI GCSGC 1 cut(s) 552
TfiI GAWTC 2 cut(s) 336, 714
Tru1I TTAA 6 cut(s) 32, 123, 128, 489, 572, 707
Tru9I TTAA 6 cut(s) 32, 123, 128, 489, 572, 707
TscAI CASTG 1 cut(s) 13
TseI GCWGC 2 cut(s) 133, 401
TspDTI ATGAA 1 cut(s) 465
TspMI CCCGGG 1 cut(s) 363
TspRI CASTG 1 cut(s) 13
Van91I CCANNNNNTGG 1 cut(s) 312
VspI ATTAAT 1 cut(s) 489
XmaI CCCGGG 1 cut(s) 363
XmiI GTMKAC 3 cut(s) 226, 359, 439
XspI CTAG 1 cut(s) 721
ZrmI AGTACT 1 cut(s) 71
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.