Rh6AG070300

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000144
Physical Location & Seq
Forward (+)
10074780 .. 10075620
841 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG070300.1

Sequence Viewer

Length: 834 bp
ATGGTCAAGGAAAAAGGATTACAGTCTACGATGTGTCTACTTAACCCAATTTCAGGAGCCCTACACAAACTTCCGCCGTTGAGGACAATTCCATCTTTCAAAAAGATTGTAAAAACGAGGCAATGGAAACGTTTTGGTGCCAGTATTTTCCTCTGGTATGTTGCATTACTTACCCCTCATGATCTTAATTCTTCAGGCCATTTTACGGTAGCCATAATCTTTGAGGATTTCAAGGGCCAGAAGACATTGGGTTTATGCAAACTTGAAGACCTAACATGGAGTCTATTCCGGGTATTCGACAACAAGGACCGCCTTGAGCTTGTGGATATACTGTTTTCTCGAGGCATTCTGTATGCTTTAGTCGATAGTCATAAGGATGGCTTGGTAGCAGCTACTCGTGTTTTAAACTTTGCAGATCATGGAGTGAAGTTGAAATTGATTCACGACAAGCAAGAACACAAGAATGTGAGAATTGATTATTTGAATAGTGACTATCCGAGAGTTTCCAATGCAAACTATCGCTCAATGTTGCTAGAATCCACCAGCAATGAAGTCTTGGTAATCCATCAAATGGTAGATTATGTTTTGTGCAGAGAAGGTGCTGGTGATATCAATGACATTGAAGAAGACGACGGCAATATTATTGTGGATAATCAGGAAGGCGTTGATCAAGATAATGATGGTGATGAAGGCAATCATGAGAATGATCACGAGGAAAGCAACTTGGAAGCTAATAATCAAGATGGAGGAGAAATTGATGATGTTGAATATGTTATTGATGAAGACGCGCCCGGCTTTAATCCACATGTCACAACTCACAACAAGGAGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

277

Amino Acids

31.45

Weight (kDa)

4.89

Isoelectric Point (pI)

32.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 8 - 190 6.8e-10 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 137
AccB7I CCANNNNNTGG 1 cut(s) 571
AccI GTMKAC 2 cut(s) 26, 37
AccII CGCG 1 cut(s) 788
AciI CCGC 2 cut(s) 74, 310
AclI AACGTT 1 cut(s) 130
AcuI CTGAAG 1 cut(s) 177
AfiI CCNNNNNNNGG 3 cut(s) 53, 205, 571
AflIII ACRYGT 1 cut(s) 805
AgsI TTSAA 7 cut(s) 100, 232, 266, 433, 484, 623, 767
AluBI AGCT 3 cut(s) 319, 392, 731
AluI AGCT 3 cut(s) 319, 392, 731
Ama87I CYCGRG 1 cut(s) 339
AoxI GGCC 2 cut(s) 196, 235
ApeKI GCWGC 1 cut(s) 389
AspLEI GCGC 1 cut(s) 790
AspS9I GGNCC 2 cut(s) 235, 307
AsuC2I CCSGG 2 cut(s) 290, 792
AsuHPI GGTGA 2 cut(s) 617, 695
AvaI CYCGRG 1 cut(s) 339
AvaII GGWCC 1 cut(s) 307
BanI GGYRCC 1 cut(s) 137
BanII GRGCYC 1 cut(s) 61
BauI CACGAG 2 cut(s) 396, 710
BbsI GAAGAC 4 cut(s) 248, 273, 633, 789
BbvI GCAGC 1 cut(s) 401
BccI CCATC 5 cut(s) 100, 371, 573, 674, 737
BceAI ACGGC 2 cut(s) 61, 649
BclI TGATCA 2 cut(s) 667, 706
BcnI CCSGG 2 cut(s) 290, 792
BfaI CTAG 1 cut(s) 533
BisI GCNGC 1 cut(s) 390
BlsI GCNGC 1 cut(s) 391
Bme1390I CCNGG 2 cut(s) 290, 792
Bme18I GGWCC 1 cut(s) 307
BmeT110I CYCGRG 1 cut(s) 339
BmgT120I GGNCC 2 cut(s) 235, 307
BmiI GGNNCC 2 cut(s) 58, 139
BmrFI CCNGG 2 cut(s) 290, 792
BpiI GAAGAC 4 cut(s) 248, 273, 633, 789
BpuEI CTTGAG 1 cut(s) 335
BpuMI CCSGG 2 cut(s) 290, 792
Bsc4I CCNNNNNNNGG 3 cut(s) 53, 205, 571
Bse1I ACTGG 1 cut(s) 141
Bse3DI GCAATG 2 cut(s) 128, 553
BseGI GGATG 1 cut(s) 382
BseLI CCNNNNNNNGG 3 cut(s) 53, 205, 571
BseMI GCAATG 2 cut(s) 128, 553
BseNI ACTGG 1 cut(s) 141
BseRI GAGGAG 1 cut(s) 762
BseXI GCAGC 1 cut(s) 401
BsgI GTGCAG 1 cut(s) 610
Bsh1236I CGCG 1 cut(s) 788
BshFI GGCC 2 cut(s) 198, 237
BshNI GGYRCC 1 cut(s) 137
BsiHKCI CYCGRG 1 cut(s) 339
BsiSI CCGG 2 cut(s) 289, 792
BslI CCNNNNNNNGG 3 cut(s) 53, 205, 571
BsmI GAATGC 1 cut(s) 345
BsnI GGCC 2 cut(s) 198, 237
BsoBI CYCGRG 1 cut(s) 339
Bsp1286I GDGCHC 1 cut(s) 61
Bsp143I GATC 4 cut(s) 181, 415, 667, 706
BspACI CCGC 2 cut(s) 74, 310
BspANI GGCC 2 cut(s) 198, 237
BspFNI CGCG 1 cut(s) 788
BspHI TCATGA 2 cut(s) 178, 697
BspLI GGNNCC 2 cut(s) 58, 139
BspT107I GGYRCC 1 cut(s) 137
BsrDI GCAATG 2 cut(s) 128, 553
BsrI ACTGG 1 cut(s) 141
BssMI GATC 4 cut(s) 181, 415, 667, 706
BssSI CACGAG 2 cut(s) 396, 710
Bst2BI CACGAG 2 cut(s) 396, 710
Bst4CI ACNGT 3 cut(s) 24, 208, 333
BstF5I GGATG 1 cut(s) 382
BstFNI CGCG 1 cut(s) 788
BstHHI GCGC 1 cut(s) 790
BstKTI GATC 4 cut(s) 184, 418, 670, 709
BstMBI GATC 4 cut(s) 181, 415, 667, 706
BstNSI RCATGY 1 cut(s) 809
BstSCI CCNGG 2 cut(s) 288, 790
BstUI CGCG 1 cut(s) 788
BstV1I GCAGC 1 cut(s) 401
BstV2I GAAGAC 4 cut(s) 248, 273, 633, 789
BsuRI GGCC 2 cut(s) 198, 237
BtsCI GGATG 1 cut(s) 382
CciI TCATGA 2 cut(s) 178, 697
CfoI GCGC 1 cut(s) 790
Cfr13I GGNCC 2 cut(s) 235, 307
CseI GACGC 1 cut(s) 794
CviAII CATG 5 cut(s) 179, 276, 419, 698, 806
CviJI RGCY 9 cut(s) 59, 198, 212, 237, 319, 381, 392, 731, 795
CviKI_1 RGCY 9 cut(s) 59, 198, 212, 237, 319, 381, 392, 731, 795
DpnI GATC 4 cut(s) 183, 417, 669, 708
DpnII GATC 4 cut(s) 181, 415, 667, 706
DraI TTTAAA 1 cut(s) 405
EciI GGCGGA 1 cut(s) 63
Eco24I GRGCYC 1 cut(s) 61
Eco32I GATATC 1 cut(s) 610
Eco47I GGWCC 1 cut(s) 307
Eco57I CTGAAG 1 cut(s) 177
Eco88I CYCGRG 1 cut(s) 339
EcoRV GATATC 1 cut(s) 610
EcoT38I GRGCYC 1 cut(s) 61
FaeI CATG 5 cut(s) 182, 279, 422, 701, 809
FalI AAGNNNNNCTT 2 cut(s) 365, 397
FatI CATG 5 cut(s) 178, 275, 418, 697, 805
FbaI TGATCA 2 cut(s) 667, 706
FblI GTMKAC 2 cut(s) 26, 37
Fnu4HI GCNGC 1 cut(s) 390
FokI GGATG 1 cut(s) 389
FriOI GRGCYC 1 cut(s) 61
Fsp4HI GCNGC 1 cut(s) 390
FspBI CTAG 1 cut(s) 533
GlaI GCGC 1 cut(s) 789
GluI GCNGC 1 cut(s) 390
HaeIII GGCC 2 cut(s) 198, 237
HapII CCGG 2 cut(s) 289, 792
HgaI GACGC 1 cut(s) 794
HhaI GCGC 1 cut(s) 790
Hin1II CATG 5 cut(s) 182, 279, 422, 701, 809
Hin6I GCGC 1 cut(s) 788
HinP1I GCGC 1 cut(s) 788
HinfI GANTC 3 cut(s) 280, 439, 536
HpaII CCGG 2 cut(s) 289, 792
HphI GGTGA 2 cut(s) 617, 695
Hpy166II GTNNAC 2 cut(s) 27, 38
Hpy188I TCNGA 1 cut(s) 498
Hpy188III TCNNGA 9 cut(s) 54, 179, 339, 443, 656, 671, 698, 710, 740
Hpy8I GTNNAC 2 cut(s) 27, 38
Hpy99I CGWCG 1 cut(s) 635
HpyAV CCTTC 3 cut(s) 590, 653, 683
HpyCH4III ACNGT 3 cut(s) 24, 208, 333
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 5 cut(s) 164, 258, 413, 512, 591
HpySE526I ACGT 1 cut(s) 130
Hsp92II CATG 5 cut(s) 182, 279, 422, 701, 809
HspAI GCGC 1 cut(s) 788
Ksp22I TGATCA 2 cut(s) 667, 706
Kzo9I GATC 4 cut(s) 181, 415, 667, 706
LmnI GCTCC 1 cut(s) 56
Lsp1109I GCAGC 1 cut(s) 401
MaeI CTAG 1 cut(s) 533
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 2 cut(s) 488, 808
MalI GATC 4 cut(s) 183, 417, 669, 708
MboI GATC 4 cut(s) 181, 415, 667, 706
MboII GAAGA 6 cut(s) 183, 253, 278, 635, 638, 794
MhlI GDGCHC 1 cut(s) 61
MluCI AATT 6 cut(s) 48, 87, 187, 434, 471, 753
MlyI GAGTC 1 cut(s) 289
MnlI CCTC 8 cut(s) 75, 111, 161, 186, 217, 335, 706, 740
MseI TTAA 4 cut(s) 42, 186, 404, 798
MslI CAYNNNNRTG 3 cut(s) 375, 462, 702
MspI CCGG 2 cut(s) 289, 792
MspR9I CCNGG 2 cut(s) 290, 792
Mva1269I GAATGC 1 cut(s) 345
MvnI CGCG 1 cut(s) 788
NciI CCSGG 2 cut(s) 290, 792
NdeII GATC 4 cut(s) 181, 415, 667, 706
NlaIII CATG 5 cut(s) 182, 279, 422, 701, 809
NlaIV GGNNCC 2 cut(s) 58, 139
NmuCI GTSAC 2 cut(s) 488, 808
NspI RCATGY 1 cut(s) 809
PaeR7I CTCGAG 1 cut(s) 339
PagI TCATGA 2 cut(s) 178, 697
PciI ACATGT 1 cut(s) 805
PctI GAATGC 1 cut(s) 345
PfeI GAWTC 2 cut(s) 439, 536
PflMI CCANNNNNTGG 1 cut(s) 571
PkrI GCNGC 1 cut(s) 391
PleI GAGTC 1 cut(s) 288
PpsI GAGTC 1 cut(s) 288
PscI ACATGT 1 cut(s) 805
Psp1406I AACGTT 1 cut(s) 130
PspN4I GGNNCC 2 cut(s) 58, 139
PspPI GGNCC 2 cut(s) 235, 307
RseI CAYNNNNRTG 3 cut(s) 375, 462, 702
SaqAI TTAA 4 cut(s) 42, 186, 404, 798
SatI GCNGC 1 cut(s) 390
Sau3AI GATC 4 cut(s) 181, 415, 667, 706
Sau96I GGNCC 2 cut(s) 235, 307
SchI GAGTC 1 cut(s) 289
ScrFI CCNGG 2 cut(s) 290, 792
SduI GDGCHC 1 cut(s) 61
SetI ASST 6 cut(s) 133, 273, 321, 394, 601, 733
Sfr274I CTCGAG 1 cut(s) 339
SinI GGWCC 1 cut(s) 307
SlaI CTCGAG 1 cut(s) 339
SmiMI CAYNNNNRTG 3 cut(s) 375, 462, 702
SmlI CTYRAG 2 cut(s) 314, 339
SmoI CTYRAG 2 cut(s) 314, 339
Sse9I AATT 6 cut(s) 48, 87, 187, 434, 471, 753
SsiI CCGC 2 cut(s) 74, 310
SspI AATATT 1 cut(s) 640
SspMI CTAG 1 cut(s) 533
StyD4I CCNGG 2 cut(s) 288, 790
TaaI ACNGT 3 cut(s) 24, 208, 333
TaiI ACGT 1 cut(s) 133
TaqI TCGA 3 cut(s) 297, 340, 363
TasI AATT 6 cut(s) 48, 87, 187, 434, 471, 753
TfiI GAWTC 2 cut(s) 439, 536
Tru1I TTAA 4 cut(s) 42, 186, 404, 798
Tru9I TTAA 4 cut(s) 42, 186, 404, 798
TseFI GTSAC 2 cut(s) 488, 808
TseI GCWGC 1 cut(s) 389
Tsp45I GTSAC 2 cut(s) 488, 808
TspDTI ATGAA 3 cut(s) 564, 702, 795
Van91I CCANNNNNTGG 1 cut(s) 571
VpaK11BI GGWCC 1 cut(s) 307
XceI RCATGY 1 cut(s) 809
XhoI CTCGAG 1 cut(s) 339
XmiI GTMKAC 2 cut(s) 26, 37
XspI CTAG 1 cut(s) 533
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.