Rorug07G0074300

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0000144
Physical Location & Seq
Reverse (-)
5724965 .. 5727703
2739 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0074300.1

Sequence Viewer

Length: 1296 bp
ATGGACAAAGCTTTAAAAACCAAGCCTTGCAGACTTGGTGATCAGAATACCAGCGACGTTGTCATCTGCTTGAAGGACAGAGAAGGAAGACACCAACAGTTTTACTCCCACTCATCTGTTCTCATAAAAAGGAGCAAGTACTTTGCTGAACGGTTGTCTAATCCAGACCCTAGTAATTCCATTAATGTTCATTGCTCTGAGGTCAACTATGATCACCATGTCAACCTGTTGAGGCTGCTCTATCTTCCAACAGAGTTGATTTTGGACGCATTGGACTCTGTTAAGTCTGCTGTCGGTATTCTTCAGTCAGCAGTTTCCTTCAAGTGTGACGAGATTGTGTCCTGCTGCATCCAGTACTTGGAGGCTGTTCCTTGGGAGGACAAAGAAGAAGAGCACATCTTAAAAGCTGTATCAAAGTTGGGTCCTATAGCTATGCCCATAATAGCTAGGATCCAACCAGTTGATTTAGCTGCCACCAAAACTGTTTTGATCTCAGCAATTCGTGTTGCCACATCCATTGTTGGACCATGTCCTCCATTCGGCAATGAGCTTAAAACTTCTGCTCAAGAGCAAGTCGAATACATGTTAGGAGAAGATGAGGATACACCGTTGGTCACAGCAGATGATGAAGTCAGATCAGTGGTAAGAACAGGTCTGTCACATATTTGTGCAACATTTGAAAAGGATTTGTCTTCCTTACTTTTGGAGCCAGACCTTGCATCCGAGACAGCAGATGTGAAAATAATGCATAGTTTATCGGATCTTGAATGGATGTGTAGCATACTCGGAAAGATGGATTTGATGAAGGATTTCGTTTCCAACTGGGCTGAAAGCTCCAGTAATGTTTTAGGAGTGATCGAGGACAAGAAACTTGATTCGTTTATGTGGGGCTTGAAGATTAAGCTCATAGAAGTCACTGCTAAAGTCTTGGAAGCAGTTGGGTATGGTAATGTGATTCTTCCAGCTCCAATTCGCTTGACATTACTGAAGTCATGGCTCCCTTATATTAGGAAGATGAAGCCACTCTTGGATAAAAGGGGCAGCGAGGACACAGGTTTTCCATACAAGATGAACGAAGACTTGTGCCAGACCATCGAAGGTGCCATTGTCACCTTAGTACTAGCGTTACCATCAAATGATCAAGCCGACATTCTGGCGGACTGGATGAGAGCCGAGCAGGTTCGGTATCCGGACTTGAGCGAGGCTTTCGAGGTCTGGTGTTTCAGAACAAAGTCCGCAAAGAGAAGATTAGTGGAGGGATTGGATGGGGCTGGCAATGCCACACTTACGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

431

Amino Acids

48.01

Weight (kDa)

5.12

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB PF00651 16 - 121 2.1e-08 BTB/POZ domain
BTB-POZ_ANK-like PF25553 244 - 409 7.2e-09 BTB/POZ Ankyrin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1168
AccB1I GGYRCC 1 cut(s) 1100
AccB7I CCANNNNNTGG 1 cut(s) 358
AccIII TCCGGA 1 cut(s) 1189
AciI CCGC 2 cut(s) 1157, 1236
AclWI GGATC 3 cut(s) 445, 458, 768
AcuI CTGAAG 2 cut(s) 287, 1007
AfaI GTAC 3 cut(s) 140, 356, 1119
AfiI CCNNNNNNNGG 2 cut(s) 358, 539
AflIII ACRYGT 1 cut(s) 582
AgsI TTSAA 5 cut(s) 73, 322, 680, 767, 895
AjuI GAANNNNNNNTTGG 2 cut(s) 1010, 1042
AluBI AGCT 9 cut(s) 11, 407, 431, 446, 470, 550, 834, 904, 965
AluI AGCT 9 cut(s) 11, 407, 431, 446, 470, 550, 834, 904, 965
Alw21I GWGCWC 1 cut(s) 396
Alw26I GTCTC 1 cut(s) 719
AlwI GGATC 3 cut(s) 445, 458, 768
Aor13HI TCCGGA 1 cut(s) 1189
ApeKI GCWGC 4 cut(s) 235, 345, 470, 1041
AseI ATTAAT 1 cut(s) 183
Asp700I GAANNNNTTC 1 cut(s) 809
AspS9I GGNCC 2 cut(s) 422, 524
AsuHPI GGTGA 3 cut(s) 50, 206, 1102
AvaII GGWCC 2 cut(s) 422, 524
BamHI GGATCC 1 cut(s) 450
BanI GGYRCC 1 cut(s) 1100
BbsI GAAGAC 3 cut(s) 94, 684, 1083
Bbv12I GWGCWC 1 cut(s) 396
BbvI GCAGC 4 cut(s) 222, 332, 457, 1053
BccI CCATC 4 cut(s) 787, 1100, 1138, 1259
BciVI GTATCC 2 cut(s) 595, 1197
BclI TGATCA 3 cut(s) 40, 211, 1138
BcoDI GTCTC 1 cut(s) 719
BfaI CTAG 3 cut(s) 171, 447, 1121
BfmI CTRYAG 1 cut(s) 426
BfuAI ACCTGC 1 cut(s) 1168
BfuI GTATCC 2 cut(s) 595, 1197
BisI GCNGC 4 cut(s) 236, 346, 471, 1042
BlsI GCNGC 4 cut(s) 237, 347, 472, 1043
BmcAI AGTACT 3 cut(s) 140, 356, 1119
Bme18I GGWCC 2 cut(s) 422, 524
BmgT120I GGNCC 2 cut(s) 422, 524
BmiI GGNNCC 5 cut(s) 423, 452, 708, 998, 1102
BmrI ACTGGG 1 cut(s) 832
BmsI GCATC 2 cut(s) 357, 728
BmuI ACTGGG 1 cut(s) 832
BpiI GAAGAC 3 cut(s) 94, 684, 1083
BpmI CTGGAG 1 cut(s) 820
BpuEI CTTGAG 2 cut(s) 549, 1216
BsaJI CCNNGG 1 cut(s) 371
BsaWI WCCGGW 1 cut(s) 1189
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 539
Bse1I ACTGG 5 cut(s) 352, 458, 827, 837, 1166
Bse3DI GCAATG 3 cut(s) 190, 550, 1282
BseAI TCCGGA 1 cut(s) 1189
BseDI CCNNGG 1 cut(s) 371
BseGI GGATG 6 cut(s) 348, 512, 719, 777, 1170, 1270
BseLI CCNNNNNNNGG 2 cut(s) 358, 539
BseMI GCAATG 3 cut(s) 190, 550, 1282
BseMII CTCAG 2 cut(s) 189, 507
BseNI ACTGG 5 cut(s) 352, 458, 827, 837, 1166
BseXI GCAGC 4 cut(s) 222, 332, 457, 1053
BshNI GGYRCC 1 cut(s) 1100
BsiHKAI GWGCWC 1 cut(s) 396
BsiSI CCGG 1 cut(s) 1190
BslI CCNNNNNNNGG 2 cut(s) 358, 539
BsmAI GTCTC 1 cut(s) 719
Bsp1286I GDGCHC 1 cut(s) 396
Bsp13I TCCGGA 1 cut(s) 1189
Bsp143I GATC 8 cut(s) 40, 211, 450, 489, 635, 760, 855, 1138
BspACI CCGC 2 cut(s) 1157, 1236
BspCNI CTCAG 2 cut(s) 190, 506
BspEI TCCGGA 1 cut(s) 1189
BspLI GGNNCC 5 cut(s) 423, 452, 708, 998, 1102
BspMI ACCTGC 1 cut(s) 1168
BspPI GGATC 3 cut(s) 445, 458, 768
BspQI GCTCTTC 1 cut(s) 384
BspT107I GGYRCC 1 cut(s) 1100
BsrDI GCAATG 3 cut(s) 190, 550, 1282
BsrI ACTGG 5 cut(s) 352, 458, 827, 837, 1166
BssECI CCNNGG 1 cut(s) 371
BssMI GATC 8 cut(s) 40, 211, 450, 489, 635, 760, 855, 1138
BssT1I CCWWGG 1 cut(s) 371
Bst4CI ACNGT 4 cut(s) 99, 153, 484, 609
Bst6I CTCTTC 1 cut(s) 384
BstC8I GCNNGC 1 cut(s) 1273
BstDEI CTNAG 3 cut(s) 198, 493, 1114
BstF5I GGATG 6 cut(s) 348, 512, 719, 777, 1170, 1270
BstKTI GATC 8 cut(s) 43, 214, 453, 492, 638, 763, 858, 1141
BstMAI GTCTC 1 cut(s) 719
BstMBI GATC 8 cut(s) 40, 211, 450, 489, 635, 760, 855, 1138
BstMWI GCNNNNNNNGC 1 cut(s) 1277
BstNSI RCATGY 1 cut(s) 586
BstSFI CTRYAG 1 cut(s) 426
BstV1I GCAGC 4 cut(s) 222, 332, 457, 1053
BstV2I GAAGAC 3 cut(s) 94, 684, 1083
BstX2I RGATCY 2 cut(s) 450, 760
BstYI RGATCY 2 cut(s) 450, 760
BsuI GTATCC 2 cut(s) 595, 1197
BtsCI GGATG 6 cut(s) 348, 512, 719, 777, 1170, 1270
BtsI GCAGTG 1 cut(s) 915
BtsIMutI CAGTG 2 cut(s) 645, 915
BveI ACCTGC 1 cut(s) 1168
Cac8I GCNNGC 1 cut(s) 1273
Cfr13I GGNCC 2 cut(s) 422, 524
CseI GACGC 1 cut(s) 275
Csp6I GTAC 3 cut(s) 139, 355, 1118
CspCI CAANNNNNGTGG 2 cut(s) 499, 534
CviAII CATG 4 cut(s) 218, 528, 583, 993
CviQI GTAC 3 cut(s) 139, 355, 1118
DdeI CTNAG 3 cut(s) 198, 493, 1114
DpnI GATC 8 cut(s) 42, 213, 452, 491, 637, 762, 857, 1140
DpnII GATC 8 cut(s) 40, 211, 450, 489, 635, 760, 855, 1138
DraI TTTAAA 1 cut(s) 15
Eam1104I CTCTTC 1 cut(s) 384
EarI CTCTTC 1 cut(s) 384
EciI GGCGGA 1 cut(s) 1172
Eco130I CCWWGG 1 cut(s) 371
Eco47I GGWCC 2 cut(s) 422, 524
Eco57I CTGAAG 2 cut(s) 287, 1007
EcoO109I RGGNCCY 1 cut(s) 422
EcoT14I CCWWGG 1 cut(s) 371
EcoT22I ATGCAT 1 cut(s) 750
ErhI CCWWGG 1 cut(s) 371
FaeI CATG 4 cut(s) 221, 531, 586, 996
FalI AAGNNNNNCTT 2 cut(s) 1010, 1042
FatI CATG 4 cut(s) 217, 527, 582, 992
FbaI TGATCA 3 cut(s) 40, 211, 1138
Fnu4HI GCNGC 4 cut(s) 236, 346, 471, 1042
FokI GGATG 6 cut(s) 335, 499, 706, 784, 1177, 1277
Fsp4HI GCNGC 4 cut(s) 236, 346, 471, 1042
FspBI CTAG 3 cut(s) 171, 447, 1121
GluI GCNGC 4 cut(s) 236, 346, 471, 1042
GsuI CTGGAG 1 cut(s) 820
HapII CCGG 1 cut(s) 1190
HgaI GACGC 1 cut(s) 275
Hin1II CATG 4 cut(s) 221, 531, 586, 996
HincII GTYRAC 2 cut(s) 205, 223
HindII GTYRAC 2 cut(s) 205, 223
HindIII AAGCTT 1 cut(s) 9
HinfI GANTC 3 cut(s) 275, 875, 955
HpaII CCGG 1 cut(s) 1190
HphI GGTGA 3 cut(s) 50, 206, 1102
Hpy166II GTNNAC 2 cut(s) 205, 223
Hpy188I TCNGA 8 cut(s) 45, 199, 635, 724, 760, 788, 1226, 1295
Hpy188III TCNNGA 4 cut(s) 164, 566, 764, 1190
Hpy8I GTNNAC 2 cut(s) 205, 223
Hpy99I CGWCG 1 cut(s) 59
HpyAV CCTTC 5 cut(s) 67, 77, 328, 799, 1091
HpyCH4III ACNGT 4 cut(s) 99, 153, 484, 609
HpyCH4IV ACGT 1 cut(s) 57
HpyCH4V TGCA 5 cut(s) 30, 348, 671, 719, 748
HpyF10VI GCNNNNNNNGC 1 cut(s) 1277
HpyF3I CTNAG 3 cut(s) 198, 493, 1114
HpySE526I ACGT 1 cut(s) 57
Hsp92II CATG 4 cut(s) 221, 531, 586, 996
Kpn2I TCCGGA 1 cut(s) 1189
Ksp22I TGATCA 3 cut(s) 40, 211, 1138
Kzo9I GATC 8 cut(s) 40, 211, 450, 489, 635, 760, 855, 1138
LguI GCTCTTC 1 cut(s) 384
LmnI GCTCC 5 cut(s) 132, 706, 839, 970, 1002
Lsp1109I GCAGC 4 cut(s) 222, 332, 457, 1053
LweI GCATC 2 cut(s) 357, 728
MaeI CTAG 3 cut(s) 171, 447, 1121
MaeII ACGT 1 cut(s) 57
MaeIII GTNAC 6 cut(s) 326, 613, 657, 913, 1108, 1125
MalI GATC 8 cut(s) 42, 213, 452, 491, 637, 762, 857, 1140
MboI GATC 8 cut(s) 40, 211, 450, 489, 635, 760, 855, 1138
MflI RGATCY 2 cut(s) 450, 760
MhlI GDGCHC 1 cut(s) 396
MluCI AATT 3 cut(s) 175, 498, 969
MlyI GAGTC 1 cut(s) 269
MmeI TCCRAC 4 cut(s) 272, 478, 502, 843
Mph1103I ATGCAT 1 cut(s) 750
MroI TCCGGA 1 cut(s) 1189
MroXI GAANNNNTTC 1 cut(s) 809
MseI TTAA 6 cut(s) 14, 183, 282, 401, 552, 900
MslI CAYNNNNRTG 1 cut(s) 666
MspI CCGG 1 cut(s) 1190
MwoI GCNNNNNNNGC 1 cut(s) 1277
NdeII GATC 8 cut(s) 40, 211, 450, 489, 635, 760, 855, 1138
NlaIII CATG 4 cut(s) 221, 531, 586, 996
NlaIV GGNNCC 5 cut(s) 423, 452, 708, 998, 1102
NmeAIII GCCGAG 1 cut(s) 1198
NmuCI GTSAC 5 cut(s) 326, 613, 657, 913, 1108
NsiI ATGCAT 1 cut(s) 750
NspI RCATGY 1 cut(s) 586
PciI ACATGT 1 cut(s) 582
PciSI GCTCTTC 1 cut(s) 384
PdmI GAANNNNTTC 1 cut(s) 809
PfeI GAWTC 2 cut(s) 875, 955
PflFI GACNNNGTC 2 cut(s) 59, 528
PflMI CCANNNNNTGG 1 cut(s) 358
PkrI GCNGC 4 cut(s) 237, 347, 472, 1043
PleI GAGTC 1 cut(s) 269
PpsI GAGTC 1 cut(s) 269
PpuMI RGGWCCY 1 cut(s) 422
PscI ACATGT 1 cut(s) 582
PshBI ATTAAT 1 cut(s) 183
Psp5II RGGWCCY 1 cut(s) 422
PspN4I GGNNCC 5 cut(s) 423, 452, 708, 998, 1102
PspPI GGNCC 2 cut(s) 422, 524
PspPPI RGGWCCY 1 cut(s) 422
PsuI RGATCY 2 cut(s) 450, 760
PsyI GACNNNGTC 2 cut(s) 59, 528
RsaI GTAC 3 cut(s) 140, 356, 1119
RsaNI GTAC 3 cut(s) 139, 355, 1118
RseI CAYNNNNRTG 1 cut(s) 666
SapI GCTCTTC 1 cut(s) 384
SaqAI TTAA 6 cut(s) 14, 183, 282, 401, 552, 900
SatI GCNGC 4 cut(s) 236, 346, 471, 1042
Sau3AI GATC 8 cut(s) 40, 211, 450, 489, 635, 760, 855, 1138
Sau96I GGNCC 2 cut(s) 422, 524
ScaI AGTACT 3 cut(s) 140, 356, 1119
SchI GAGTC 1 cut(s) 269
SduI GDGCHC 1 cut(s) 396
SfaNI GCATC 2 cut(s) 357, 728
SfcI CTRYAG 1 cut(s) 426
SinI GGWCC 2 cut(s) 422, 524
SmiMI CAYNNNNRTG 1 cut(s) 666
SmlI CTYRAG 2 cut(s) 564, 1195
SmoI CTYRAG 2 cut(s) 564, 1195
Sse9I AATT 3 cut(s) 175, 498, 969
SsiI CCGC 2 cut(s) 1157, 1236
SspMI CTAG 3 cut(s) 171, 447, 1121
StyI CCWWGG 1 cut(s) 371
TaaI ACNGT 4 cut(s) 99, 153, 484, 609
TaiI ACGT 1 cut(s) 60
TaqI TCGA 4 cut(s) 576, 858, 1095, 1209
TasI AATT 3 cut(s) 175, 498, 969
TatI WGTACW 3 cut(s) 138, 354, 1117
TfiI GAWTC 2 cut(s) 875, 955
Tru1I TTAA 6 cut(s) 14, 183, 282, 401, 552, 900
Tru9I TTAA 6 cut(s) 14, 183, 282, 401, 552, 900
TscAI CASTG 2 cut(s) 645, 922
TseFI GTSAC 5 cut(s) 326, 613, 657, 913, 1108
TseI GCWGC 4 cut(s) 235, 345, 470, 1041
Tsp45I GTSAC 5 cut(s) 326, 613, 657, 913, 1108
TspDTI ATGAA 5 cut(s) 179, 642, 818, 1031, 1085
TspRI CASTG 2 cut(s) 645, 922
Tth111I GACNNNGTC 2 cut(s) 59, 528
Van91I CCANNNNNTGG 1 cut(s) 358
VpaK11BI GGWCC 2 cut(s) 422, 524
VspI ATTAAT 1 cut(s) 183
XceI RCATGY 1 cut(s) 586
XmnI GAANNNNTTC 1 cut(s) 809
XspI CTAG 3 cut(s) 171, 447, 1121
ZrmI AGTACT 3 cut(s) 140, 356, 1119
Zsp2I ATGCAT 1 cut(s) 750
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.