RchiOBHm_Chr5g0063131

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000144
Physical Location & Seq
Forward (+)
68874609 .. 68876099
1491 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33927

Sequence Viewer

Length: 1491 bp
ATGGAGTTCATGATTAAAACTGTATTAGTTTGCGGTTGCTTAGTGTTTGGTGGTGTGGTGATTAAGAGACTATTCCGTCACAAGCCCTGCTGGGAGACACTACCTGACGATATCATGGAGGTTATTGTGCCACACCTGAGTGTACGTGATCGTATACGCTTAAGCCTAGTTTGCAAGCCATGGAGCTCAGTTCCTATGTGCAAAGACATCCCTAGTTCTCCTCACAAACTCCCCTGGTTAGTACTCCCTCAACCTGAATCTCAAGCTCTAACTTGCAATGATACCTCCTTAAGCTTTTACGACCTCCATGACGGCAAATTTGTTAAGTTGGAGCTGCCTGAACCTGTTCGTGGAGCGTGGTTTCGAGGGTCTTCGAAAGGTTGGTTGATTATGATTAAGGAAAGTGAACAAAACACTACAACATTTCTGGTAAATCCAATTTCAGGAGTCCAACACCAACTTCCATCCTTGAGAACTATTCCGTCTTTCCAGAAGTTTGTAGAAACGAAGAAATGGAAACGATATGGTGCCTTTGCTTTCTTCAACTGCCTTGTGCTATCTACCTCAGATATTCATTCCGATGAATGTGTGGTAGCAGCTGCTCTTGGGTGTGCCAATGATATATTGGGTTTGTGCAGACCTGGAGACAAAGAATGGAATATCTTTCGGTTATTAGATGCAGATGAAGCTTTGACTGATGTACTGTTTTGTTCTGGCAAGCTATACGCCTTGGTTGTGAGTAATAATAAGTCCGGCGTTGTTTCAACTGCTCGTACGTTGAATTTTGGAGATCATTCGGTGGAATTGAAATTAATCTATAACGCAAATATAAACTTCCATACTAACTCTGAATATCATGGTAACTATGAGATCTACGTACAAGGCTATGTTGACTCATATTTGATAGAGTCTGCAACCAATAAGGAAGTATTGTTGATCCACCAAAGGTTAGATGTATTTTCGAGCATAGGTGACAACAACAACGGAGAGAATGGTGGTGGCGGCAACCATGAGGAAGAGGAAGAGGAAGTGGAAGTGGAAGTCGAAGGCAATGACGGTGGTGATGACGCCAATGAGGAATGGAACAACTTGGGAGGAAATGGTGGTGATATAGGTGACTGGGCCATCAATATTGTTGAGGAAGATGATCATTATCTACGTACATATTTGAGGACAAGTGGTTTTGATGTATACAGGATTGATCCACAGAATGACAATTTCGAAATGGTACAAAGTTTGGGGGACAAGCTATTATTTTTGTCAAACGCTGGTTCATTTTCCCTCCGGGCAAGTGATATCAAAATCAAAGACTTCAGAGGGAATTGCATTTGTTTTGCAACAAATAATTTCATGAGTTTACATTCTTTAGAAACATATACCTGTCGGGAGATTGGTGTATTTGACTTGAATGCAAGAAGAATGCGGCGACCTTTCCCGAGTGTAGAGGTGTCAAATCGGTCTCAACTCTGTTGGTTCACTCCAATGTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

496

Amino Acids

55.96

Weight (kDa)

5.08

Isoelectric Point (pI)

40.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 99 - 468 2e-38 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1489
AasI GACNNNNNNGTC 1 cut(s) 75
AccB1I GGYRCC 1 cut(s) 527
AccI GTMKAC 2 cut(s) 154, 1191
AciI CCGC 3 cut(s) 33, 1002, 1423
AclWI GGATC 2 cut(s) 931, 1196
AcsI RAATTY 2 cut(s) 317, 781
AcuI CTGAAG 1 cut(s) 1297
AcyI GRCGYC 1 cut(s) 1068
AfaI GTAC 7 cut(s) 144, 243, 702, 775, 879, 1162, 1230
AfiI CCNNNNNNNGG 2 cut(s) 350, 443
AflII CTTAAG 2 cut(s) 160, 289
AgsI TTSAA 5 cut(s) 544, 765, 781, 808, 1408
AjnI CCWGG 2 cut(s) 233, 640
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AleI CACNNNNGTG 1 cut(s) 138
AluBI AGCT 8 cut(s) 186, 266, 294, 334, 599, 689, 721, 1249
AluI AGCT 8 cut(s) 186, 266, 294, 334, 599, 689, 721, 1249
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 4 cut(s) 61, 89, 639, 1464
AlwI GGATC 2 cut(s) 931, 1196
Ama87I CYCGRG 1 cut(s) 1435
AoxI GGCC 1 cut(s) 1122
ApeKI GCWGC 3 cut(s) 334, 596, 599
ApoI RAATTY 2 cut(s) 317, 781
ArsI GACNNNNNNTTYG 2 cut(s) 302, 334
AseI ATTAAT 1 cut(s) 812
Asp700I GAANNNNTTC 1 cut(s) 345
AspS9I GGNCC 1 cut(s) 1122
AsuC2I CCSGG 1 cut(s) 1286
AsuHPI GGTGA 5 cut(s) 70, 983, 1073, 1118, 1127
AsuII TTCGAA 2 cut(s) 374, 1221
AvaI CYCGRG 1 cut(s) 1435
BanI GGYRCC 1 cut(s) 527
BanII GRGCYC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 3 cut(s) 321, 586, 608
BccI CCATC 2 cut(s) 472, 1133
BceAI ACGGC 1 cut(s) 328
BciT130I CCWGG 2 cut(s) 235, 642
BclI TGATCA 1 cut(s) 1147
BcnI CCSGG 1 cut(s) 1286
BcoDI GTCTC 4 cut(s) 61, 89, 639, 1464
BfaI CTAG 2 cut(s) 167, 213
BfrI CTTAAG 2 cut(s) 160, 289
BglII AGATCT 1 cut(s) 870
BisI GCNGC 5 cut(s) 335, 597, 600, 1003, 1424
BlsI GCNGC 5 cut(s) 336, 598, 601, 1004, 1425
BmcAI AGTACT 1 cut(s) 243
Bme1390I CCNGG 3 cut(s) 235, 642, 1286
BmeT110I CYCGRG 1 cut(s) 1435
BmgT120I GGNCC 1 cut(s) 1122
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 3 cut(s) 235, 642, 1286
BmrI ACTGGG 1 cut(s) 1129
BmsI GCATC 1 cut(s) 667
BmuI ACTGGG 1 cut(s) 1129
BpiI GAAGAC 1 cut(s) 363
BpmI CTGGAG 1 cut(s) 663
Bpu14I TTCGAA 2 cut(s) 374, 1221
BpuEI CTTGAG 2 cut(s) 246, 490
BpuMI CCSGG 1 cut(s) 1286
BsaAI YACGTR 3 cut(s) 146, 877, 1160
BsaBI GATNNNNATC 1 cut(s) 1152
BsaHI GRCGYC 1 cut(s) 1068
BsaI GGTCTC 1 cut(s) 1464
BsaJI CCNNGG 3 cut(s) 179, 233, 729
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 443
Bse1I ACTGG 1 cut(s) 1124
Bse3DI GCAATG 2 cut(s) 283, 1057
Bse8I GATNNNNATC 1 cut(s) 1152
BseBI CCWGG 2 cut(s) 235, 642
BseDI CCNNGG 3 cut(s) 179, 233, 729
BseGI GGATG 2 cut(s) 207, 464
BseJI GATNNNNATC 1 cut(s) 1152
BseLI CCNNNNNNNGG 2 cut(s) 350, 443
BseMI GCAATG 2 cut(s) 283, 1057
BseMII CTCAG 3 cut(s) 128, 201, 579
BseNI ACTGG 1 cut(s) 1124
BseRI GAGGAG 1 cut(s) 210
BseXI GCAGC 3 cut(s) 321, 586, 608
BseYI CCCAGC 1 cut(s) 90
BsgI GTGCAG 1 cut(s) 655
BshFI GGCC 1 cut(s) 1124
BshNI GGYRCC 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 188
BsiHKCI CYCGRG 1 cut(s) 1435
BsiSI CCGG 2 cut(s) 753, 1285
BsiWI CGTACG 1 cut(s) 773
BslFI GGGAC 1 cut(s) 1256
BslI CCNNNNNNNGG 2 cut(s) 350, 443
BsmAI GTCTC 4 cut(s) 61, 89, 639, 1464
BsmFI GGGAC 1 cut(s) 1256
BsmI GAATGC 2 cut(s) 1414, 1425
BsnI GGCC 1 cut(s) 1124
Bso31I GGTCTC 1 cut(s) 1464
BsoBI CYCGRG 1 cut(s) 1435
Bsp119I TTCGAA 2 cut(s) 374, 1221
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 6 cut(s) 148, 790, 870, 936, 1147, 1201
Bsp19I CCATGG 1 cut(s) 179
BspACI CCGC 3 cut(s) 33, 1002, 1423
BspANI GGCC 1 cut(s) 1124
BspCNI CTCAG 3 cut(s) 129, 200, 578
BspHI TCATGA 2 cut(s) 9, 1350
BspLI GGNNCC 1 cut(s) 529
BspPI GGATC 2 cut(s) 931, 1196
BspT104I TTCGAA 2 cut(s) 374, 1221
BspT107I GGYRCC 1 cut(s) 527
BspTI CTTAAG 2 cut(s) 160, 289
BspTNI GGTCTC 1 cut(s) 1464
BsrDI GCAATG 2 cut(s) 283, 1057
BsrI ACTGG 1 cut(s) 1124
BssECI CCNNGG 3 cut(s) 179, 233, 729
BssMI GATC 6 cut(s) 148, 790, 870, 936, 1147, 1201
BssNAI GTATAC 2 cut(s) 155, 1192
BssNI GRCGYC 1 cut(s) 1068
BssT1I CCWWGG 2 cut(s) 179, 729
Bst1107I GTATAC 2 cut(s) 155, 1192
Bst2UI CCWGG 2 cut(s) 235, 642
Bst4CI ACNGT 3 cut(s) 22, 705, 1058
Bst6I CTCTTC 2 cut(s) 1011, 1017
BstACI GRCGYC 1 cut(s) 1068
BstAFI CTTAAG 2 cut(s) 160, 289
BstBAI YACGTR 3 cut(s) 146, 877, 1160
BstBI TTCGAA 2 cut(s) 374, 1221
BstC8I GCNNGC 2 cut(s) 176, 719
BstDEI CTNAG 4 cut(s) 40, 137, 187, 565
BstDSI CCRYGG 1 cut(s) 179
BstF5I GGATG 2 cut(s) 207, 464
BstKTI GATC 6 cut(s) 151, 793, 873, 939, 1150, 1204
BstMAI GTCTC 4 cut(s) 61, 89, 639, 1464
BstMBI GATC 6 cut(s) 148, 790, 870, 936, 1147, 1201
BstMWI GCNNNNNNNGC 2 cut(s) 171, 686
BstNI CCWGG 2 cut(s) 235, 642
BstSCI CCNGG 3 cut(s) 233, 640, 1284
BstSNI TACGTA 2 cut(s) 877, 1160
BstV1I GCAGC 3 cut(s) 321, 586, 608
BstV2I GAAGAC 1 cut(s) 363
BstX2I RGATCY 1 cut(s) 870
BstYI RGATCY 1 cut(s) 870
BstZ17I GTATAC 2 cut(s) 155, 1192
BsuRI GGCC 1 cut(s) 1124
BtgI CCRYGG 1 cut(s) 179
BtsCI GGATG 2 cut(s) 207, 464
Cac8I GCNNGC 2 cut(s) 176, 719
CciI TCATGA 2 cut(s) 9, 1350
Cfr13I GGNCC 1 cut(s) 1122
CseI GACGC 1 cut(s) 1076
Csp6I GTAC 7 cut(s) 143, 242, 701, 774, 878, 1161, 1229
CspCI CAANNNNNGTGG 2 cut(s) 1039, 1074
CviAII CATG 7 cut(s) 10, 115, 180, 308, 857, 1010, 1351
CviQI GTAC 7 cut(s) 143, 242, 701, 774, 878, 1161, 1229
DdeI CTNAG 4 cut(s) 40, 137, 187, 565
DpnI GATC 6 cut(s) 150, 792, 872, 938, 1149, 1203
DpnII GATC 6 cut(s) 148, 790, 870, 936, 1147, 1201
DrdI GACNNNNNNGTC 1 cut(s) 75
DseDI GACNNNNNNGTC 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 1011, 1017
EarI CTCTTC 2 cut(s) 1011, 1017
Ecl136II GAGCTC 1 cut(s) 186
Eco105I TACGTA 2 cut(s) 877, 1160
Eco130I CCWWGG 2 cut(s) 179, 729
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 1464
Eco32I GATATC 2 cut(s) 112, 1297
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 1297
Eco88I CYCGRG 1 cut(s) 1435
EcoICRI GAGCTC 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 233, 640
EcoRV GATATC 2 cut(s) 112, 1297
EcoT14I CCWWGG 2 cut(s) 179, 729
EcoT38I GRGCYC 1 cut(s) 188
ErhI CCWWGG 2 cut(s) 179, 729
FaeI CATG 7 cut(s) 13, 118, 183, 311, 860, 1013, 1354
FaqI GGGAC 1 cut(s) 1256
FatI CATG 7 cut(s) 9, 114, 179, 307, 856, 1009, 1350
FbaI TGATCA 1 cut(s) 1147
FblI GTMKAC 2 cut(s) 154, 1191
Fnu4HI GCNGC 5 cut(s) 335, 597, 600, 1003, 1424
FokI GGATG 2 cut(s) 194, 451
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 5 cut(s) 335, 597, 600, 1003, 1424
FspBI CTAG 2 cut(s) 167, 213
GluI GCNGC 5 cut(s) 335, 597, 600, 1003, 1424
GsaI CCCAGC 1 cut(s) 94
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 1 cut(s) 1124
HapII CCGG 2 cut(s) 753, 1285
HgaI GACGC 1 cut(s) 1076
Hin1I GRCGYC 1 cut(s) 1068
Hin1II CATG 7 cut(s) 13, 118, 183, 311, 860, 1013, 1354
HincII GTYRAC 1 cut(s) 892
HindII GTYRAC 1 cut(s) 892
HindIII AAGCTT 2 cut(s) 292, 687
HinfI GANTC 4 cut(s) 257, 447, 893, 908
HpaII CCGG 2 cut(s) 753, 1285
HphI GGTGA 5 cut(s) 70, 983, 1073, 1118, 1127
Hpy166II GTNNAC 7 cut(s) 143, 155, 407, 892, 1192, 1358, 1476
Hpy188I TCNGA 4 cut(s) 568, 580, 850, 1316
Hpy188III TCNNGA 6 cut(s) 10, 444, 490, 1351, 1385, 1435
Hpy8I GTNNAC 7 cut(s) 143, 155, 407, 892, 1192, 1358, 1476
HpyAV CCTTC 1 cut(s) 1040
HpyCH4III ACNGT 3 cut(s) 22, 705, 1058
HpyCH4IV ACGT 4 cut(s) 145, 776, 876, 1159
HpyCH4V TGCA 9 cut(s) 174, 201, 276, 636, 680, 914, 1326, 1337, 1412
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 686
HpyF3I CTNAG 4 cut(s) 40, 137, 187, 565
HpySE526I ACGT 4 cut(s) 145, 776, 876, 1159
Hsp92I GRCGYC 1 cut(s) 1068
Hsp92II CATG 7 cut(s) 13, 118, 183, 311, 860, 1013, 1354
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 6 cut(s) 148, 790, 870, 936, 1147, 1201
LmnI GCTCC 3 cut(s) 183, 331, 353
Lsp1109I GCAGC 3 cut(s) 321, 586, 608
LweI GCATC 1 cut(s) 667
MaeI CTAG 2 cut(s) 167, 213
MaeII ACGT 4 cut(s) 145, 776, 876, 1159
MaeIII GTNAC 4 cut(s) 77, 860, 971, 1115
MalI GATC 6 cut(s) 150, 792, 872, 938, 1149, 1203
MboI GATC 6 cut(s) 148, 790, 870, 936, 1147, 1201
MboII GAAGA 7 cut(s) 363, 520, 532, 1028, 1034, 1154, 1428
MflI RGATCY 1 cut(s) 870
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 8 cut(s) 317, 438, 781, 803, 809, 1216, 1321, 1345
MlyI GAGTC 3 cut(s) 456, 887, 917
MmeI TCCRAC 2 cut(s) 309, 475
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 7 cut(s) 15, 63, 161, 290, 324, 396, 812
MslI CAYNNNNRTG 3 cut(s) 138, 579, 1481
MspA1I CMGCKG 1 cut(s) 599
MspCI CTTAAG 2 cut(s) 160, 289
MspI CCGG 2 cut(s) 753, 1285
MspR9I CCNGG 3 cut(s) 235, 642, 1286
Mva1269I GAATGC 2 cut(s) 1414, 1425
MvaI CCWGG 2 cut(s) 235, 642
MwoI GCNNNNNNNGC 2 cut(s) 171, 686
NciI CCSGG 1 cut(s) 1286
NcoI CCATGG 1 cut(s) 179
NdeII GATC 6 cut(s) 148, 790, 870, 936, 1147, 1201
NlaIII CATG 7 cut(s) 13, 118, 183, 311, 860, 1013, 1354
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 3 cut(s) 77, 971, 1115
NspV TTCGAA 2 cut(s) 374, 1221
OliI CACNNNNGTG 1 cut(s) 138
PagI TCATGA 2 cut(s) 9, 1350
PctI GAATGC 2 cut(s) 1414, 1425
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 1 cut(s) 257
Pfl23II CGTACG 1 cut(s) 773
PkrI GCNGC 5 cut(s) 336, 598, 601, 1004, 1425
PleI GAGTC 3 cut(s) 455, 887, 916
PpsI GAGTC 3 cut(s) 455, 887, 916
Ppu21I YACGTR 3 cut(s) 146, 877, 1160
PshBI ATTAAT 1 cut(s) 812
PsiI TTATAA 1 cut(s) 1489
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 233, 640
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 2 cut(s) 233, 640
PspLI CGTACG 1 cut(s) 773
PspN4I GGNNCC 1 cut(s) 529
PspPI GGNCC 1 cut(s) 1122
PsuI RGATCY 1 cut(s) 870
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 7 cut(s) 144, 243, 702, 775, 879, 1162, 1230
RsaNI GTAC 7 cut(s) 143, 242, 701, 774, 878, 1161, 1229
RseI CAYNNNNRTG 3 cut(s) 138, 579, 1481
SacI GAGCTC 1 cut(s) 188
SaqAI TTAA 7 cut(s) 15, 63, 161, 290, 324, 396, 812
SatI GCNGC 5 cut(s) 335, 597, 600, 1003, 1424
Sau3AI GATC 6 cut(s) 148, 790, 870, 936, 1147, 1201
Sau96I GGNCC 1 cut(s) 1122
ScaI AGTACT 1 cut(s) 243
SchI GAGTC 3 cut(s) 456, 887, 917
ScrFI CCNGG 3 cut(s) 235, 642, 1286
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 1 cut(s) 667
SfuI TTCGAA 2 cut(s) 374, 1221
SmiMI CAYNNNNRTG 3 cut(s) 138, 579, 1481
SmlI CTYRAG 4 cut(s) 160, 261, 289, 469
SmoI CTYRAG 4 cut(s) 160, 261, 289, 469
SnaBI TACGTA 2 cut(s) 877, 1160
Sse9I AATT 8 cut(s) 317, 438, 781, 803, 809, 1216, 1321, 1345
SsiI CCGC 3 cut(s) 33, 1002, 1423
SspI AATATT 1 cut(s) 1132
SspMI CTAG 2 cut(s) 167, 213
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 3 cut(s) 233, 640, 1284
StyI CCWWGG 2 cut(s) 179, 729
TaaI ACNGT 3 cut(s) 22, 705, 1058
TaiI ACGT 4 cut(s) 148, 779, 879, 1162
TaqI TCGA 5 cut(s) 364, 374, 962, 1044, 1221
TaqII GACCGA 1 cut(s) 1446
TasI AATT 8 cut(s) 317, 438, 781, 803, 809, 1216, 1321, 1345
TatI WGTACW 2 cut(s) 241, 700
TauI GCSGC 2 cut(s) 1005, 1426
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 7 cut(s) 15, 63, 161, 290, 324, 396, 812
Tru9I TTAA 7 cut(s) 15, 63, 161, 290, 324, 396, 812
TseFI GTSAC 3 cut(s) 77, 971, 1115
TseI GCWGC 3 cut(s) 334, 596, 599
Tsp45I GTSAC 3 cut(s) 77, 971, 1115
TspDTI ATGAA 5 cut(s) 563, 597, 699, 1263, 1339
TspGWI ACGGA 3 cut(s) 65, 471, 999
Vha464I CTTAAG 2 cut(s) 160, 289
VspI ATTAAT 1 cut(s) 812
XapI RAATTY 2 cut(s) 317, 781
XcmI CCANNNNNNNNNTGG 1 cut(s) 622
XmiI GTMKAC 2 cut(s) 154, 1191
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 2 cut(s) 167, 213
ZrmI AGTACT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.