Rw6G006250

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000144
Physical Location & Seq
Reverse (-)
9875069 .. 9876547
1479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G006250.1

Sequence Viewer

Length: 1479 bp
ATGGAGATGAAGCAAAAGGAAGATCAATGCGGAAACCGAACAGATCCTACTTGTTATAACAATGGATGTAGCAGTTGGCAAACACCGCCTGCAGATATCATGGAGAAGATTCTACAATGTTTCACTACTTTAGGCGATCGGATACGGATGAGCATTGTGTGCAAGTCTTGGAAATCAATTATTATGCGAAGAGAGATCTGTGCCACTGCTCCTCCTTTATTTCCATGGTTACTACCCCCTCAAGCCCCACACTGCAGCAAGAAATACTTGACATTCGGTCCCATCTCTGAGGACAAAGTGAGTCTCAATTTGAAGCTCCCTAAGCGAGTTCGAGGAGGGTGGATTTACGGGTCGTCTAAAGGTTGGTTCATCATCGTCAAGGAAAGAGGACTAAACTCAAAGATGTTTCTACTCAACCCAATTTCAAGAGTACTACACCACCTTCCATCCTTGAGAACAATTCCATTTTTCAAGAAGGTTGTAAAAACCAGGAAATGGGAACTTTTTGGTGCCAAGGAATTCTGTTGTCACGTCGCGTTAGCTACTCACAAAGATATTAACCTTTCAGAATCATTTACAGTGGCAGCAGTTTTTAATGATAGGAAGACATTGTGTTTGTGCCGACCGGGAGACAAAGCATGGACTGTCACTCAAGTCTTAGATATCAATGAAAACTGCTTCATTTTTGATATATTATTTTCTTTTGGCACTCTATATGCTTTGGTCAGGAGTCAAAAGGAGGATGGCATCGTTGATGCAGCTACTCGCACCTTAAACGTTGGCTTTGGAGATCATGCGGTAGAATTCAAGTTGAAGTTGGTCTACGACAAGCGTGAAAACAAAAATAAGATAATTAATAAACGACGTGGAGATAAAGAAATCGTTTACAATGGAATGTACAACTCAATGTTGCTAGAATCAACGAACAGTGAGGTCTTGTTGATCCATCAAATGTTGGATTTTAGATTCACCATCAATGAAGATGATGAGGGTGATGAAGCCAACAATGAAGATCATAGTGGTGATGAAAATATTGAAAATGATGACGTTGATCAAGAAGGCAACATCGTGGGAGGTAATGATGAGGATGATGGAGAAGGTGATGGTAATGAAGATGACAATACTATTAATGAAGACTTGCCTGGCTTTAATCCAGAATTAAGGACAAGGAATTTTATAGCATACAAGATTGACACAGAAAATGACAACTTCACTCTGCTGCAAAGCTTGGGCGACCAAATATTATTTTGCGGAAACGATGGTTCATTCTCCCTTCCAGCTAGTCCTTTTGAAGAATCAAATAAGAACACCATATATTATGCAAGAAACAGTGTCTGGGACTATGAAGCAACTCCAGAAACATATACATCTCGGGAGATTGGCTTTTTTAAGTTTGGTGAAGAAAAAGCTTTTCGGTTTCCAAACTGCCACGTCATGTCAGTCCGATATCAAGGTAGTTGGTTCACTCCAAGATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

492

Amino Acids

56.34

Weight (kDa)

5.35

Isoelectric Point (pI)

40.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 95 - 463 9.9e-29 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 57
AccB1I GGYRCC 1 cut(s) 509
AccB7I CCANNNNNTGG 1 cut(s) 495
AccI GTMKAC 1 cut(s) 822
AccII CGCG 1 cut(s) 536
AciI CCGC 4 cut(s) 30, 86, 797, 1251
AclI AACGTT 1 cut(s) 777
AclWI GGATC 2 cut(s) 38, 937
AcsI RAATTY 3 cut(s) 518, 803, 1171
AfaI GTAC 2 cut(s) 432, 899
AfiI CCNNNNNNNGG 1 cut(s) 495
AgsI TTSAA 7 cut(s) 313, 426, 472, 808, 814, 1037, 1292
AhdI GACNNNNNGTC 1 cut(s) 276
AjiI CACGTC 3 cut(s) 532, 866, 1432
AjnI CCWGG 2 cut(s) 488, 1141
AluBI AGCT 6 cut(s) 316, 542, 761, 1227, 1280, 1409
AluI AGCT 6 cut(s) 316, 542, 761, 1227, 1280, 1409
Alw26I GTCTC 2 cut(s) 308, 624
AlwI GGATC 2 cut(s) 38, 937
AlwNI CAGNNNCTG 1 cut(s) 1335
Ama87I CYCGRG 1 cut(s) 1371
ApeKI GCWGC 4 cut(s) 255, 584, 758, 1219
ApoI RAATTY 3 cut(s) 518, 803, 1171
ArsI GACNNNNNNTTYG 2 cut(s) 598, 630
AseI ATTAAT 2 cut(s) 855, 1128
AspS9I GGNCC 1 cut(s) 278
AsuC2I CCSGG 1 cut(s) 627
AsuHPI GGTGA 5 cut(s) 961, 1004, 1034, 1112, 1409
AvaI CYCGRG 1 cut(s) 1371
AvaII GGWCC 1 cut(s) 278
BanI GGYRCC 1 cut(s) 509
BarI GAAGNNNNNNTAC 2 cut(s) 806, 838
BbsI GAAGAC 2 cut(s) 611, 1140
BbvI GCAGC 4 cut(s) 267, 596, 770, 1206
BccI CCATC 8 cut(s) 290, 454, 737, 954, 980, 1085, 1097, 1253
BciT130I CCWGG 2 cut(s) 490, 1143
BciVI GTATCC 1 cut(s) 135
BclI TGATCA 1 cut(s) 1051
BcnI CCSGG 1 cut(s) 627
BcoDI GTCTC 2 cut(s) 308, 624
BfaI CTAG 2 cut(s) 914, 1281
BfmI CTRYAG 2 cut(s) 90, 253
BfuI GTATCC 1 cut(s) 135
BglII AGATCT 1 cut(s) 195
BisI GCNGC 4 cut(s) 256, 585, 759, 1220
BlsI GCNGC 4 cut(s) 257, 586, 760, 1221
BmcAI AGTACT 1 cut(s) 432
Bme1390I CCNGG 3 cut(s) 490, 627, 1143
Bme18I GGWCC 1 cut(s) 278
BmeRI GACNNNNNGTC 1 cut(s) 276
BmeT110I CYCGRG 1 cut(s) 1371
BmgBI CACGTC 3 cut(s) 532, 866, 1432
BmgT120I GGNCC 1 cut(s) 278
BmiI GGNNCC 2 cut(s) 280, 511
BmrFI CCNGG 3 cut(s) 490, 627, 1143
BmsI GCATC 2 cut(s) 745, 756
BpiI GAAGAC 2 cut(s) 611, 1140
BpmI CTGGAG 1 cut(s) 1338
Bpu10I CCTNAGC 1 cut(s) 321
BpuEI CTTGAG 3 cut(s) 225, 472, 636
BpuMI CCSGG 1 cut(s) 627
BsaJI CCNNGG 2 cut(s) 224, 513
BsaXI ACNNNNNCTCC 2 cut(s) 196, 226
Bsc4I CCNNNNNNNGG 1 cut(s) 495
BseBI CCWGG 2 cut(s) 490, 1143
BseDI CCNNGG 2 cut(s) 224, 513
BseGI GGATG 5 cut(s) 71, 153, 446, 748, 1093
BseLI CCNNNNNNNGG 1 cut(s) 495
BseMII CTCAG 1 cut(s) 279
BseRI GAGGAG 2 cut(s) 201, 348
BseXI GCAGC 4 cut(s) 267, 596, 770, 1206
Bsh1236I CGCG 1 cut(s) 536
Bsh1285I CGRYCG 2 cut(s) 139, 626
BshNI GGYRCC 1 cut(s) 509
BsiEI CGRYCG 2 cut(s) 139, 626
BsiHKCI CYCGRG 1 cut(s) 1371
BsiSI CCGG 1 cut(s) 626
BslFI GGGAC 2 cut(s) 264, 1352
BslI CCNNNNNNNGG 1 cut(s) 495
BsmAI GTCTC 2 cut(s) 308, 624
BsmFI GGGAC 2 cut(s) 264, 1352
BsoBI CYCGRG 1 cut(s) 1371
Bsp1407I TGTACA 1 cut(s) 897
Bsp143I GATC 8 cut(s) 22, 43, 136, 195, 790, 942, 1012, 1051
Bsp19I CCATGG 1 cut(s) 224
BspACI CCGC 4 cut(s) 30, 86, 797, 1251
BspCNI CTCAG 1 cut(s) 280
BspFNI CGCG 1 cut(s) 536
BspLI GGNNCC 2 cut(s) 280, 511
BspMAI CTGCAG 2 cut(s) 94, 257
BspPI GGATC 2 cut(s) 38, 937
BspT107I GGYRCC 1 cut(s) 509
BsrGI TGTACA 1 cut(s) 897
BssECI CCNNGG 2 cut(s) 224, 513
BssMI GATC 8 cut(s) 22, 43, 136, 195, 790, 942, 1012, 1051
BssT1I CCWWGG 2 cut(s) 224, 513
Bst2UI CCWGG 2 cut(s) 490, 1143
Bst4CI ACNGT 4 cut(s) 580, 646, 929, 1331
Bst6I CTCTTC 1 cut(s) 184
BstAPI GCANNNNNTGC 1 cut(s) 159
BstAUI TGTACA 1 cut(s) 897
BstC8I GCNNGC 1 cut(s) 90
BstDEI CTNAG 3 cut(s) 288, 321, 658
BstDSI CCRYGG 1 cut(s) 224
BstF5I GGATG 5 cut(s) 71, 153, 446, 748, 1093
BstFNI CGCG 1 cut(s) 536
BstKTI GATC 8 cut(s) 25, 46, 139, 198, 793, 945, 1015, 1054
BstMAI GTCTC 2 cut(s) 308, 624
BstMBI GATC 8 cut(s) 22, 43, 136, 195, 790, 942, 1012, 1051
BstMCI CGRYCG 2 cut(s) 139, 626
BstMWI GCNNNNNNNGC 3 cut(s) 85, 159, 322
BstNI CCWGG 2 cut(s) 490, 1143
BstSCI CCNGG 3 cut(s) 488, 625, 1141
BstSFI CTRYAG 2 cut(s) 90, 253
BstUI CGCG 1 cut(s) 536
BstV1I GCAGC 4 cut(s) 267, 596, 770, 1206
BstV2I GAAGAC 2 cut(s) 611, 1140
BstX2I RGATCY 2 cut(s) 43, 195
BstYI RGATCY 2 cut(s) 43, 195
BsuI GTATCC 1 cut(s) 135
BtgI CCRYGG 1 cut(s) 224
BtrI CACGTC 3 cut(s) 532, 866, 1432
BtsCI GGATG 5 cut(s) 71, 153, 446, 748, 1093
BtsI GCAGTG 2 cut(s) 204, 250
BtsIMutI CAGTG 5 cut(s) 204, 250, 585, 934, 1336
Cac8I GCNNGC 1 cut(s) 90
CaiI CAGNNNCTG 1 cut(s) 1335
Cfr13I GGNCC 1 cut(s) 278
Csp6I GTAC 2 cut(s) 431, 898
CviAII CATG 5 cut(s) 100, 225, 639, 794, 1435
CviQI GTAC 2 cut(s) 431, 898
DdeI CTNAG 3 cut(s) 288, 321, 658
DpnI GATC 8 cut(s) 24, 45, 138, 197, 792, 944, 1014, 1053
DpnII GATC 8 cut(s) 22, 43, 136, 195, 790, 942, 1012, 1051
DriI GACNNNNNGTC 1 cut(s) 276
Eam1104I CTCTTC 1 cut(s) 184
Eam1105I GACNNNNNGTC 1 cut(s) 276
EarI CTCTTC 1 cut(s) 184
Eco130I CCWWGG 2 cut(s) 224, 513
Eco32I GATATC 3 cut(s) 97, 664, 1448
Eco47I GGWCC 1 cut(s) 278
Eco88I CYCGRG 1 cut(s) 1371
EcoRI GAATTC 2 cut(s) 518, 803
EcoRII CCWGG 2 cut(s) 488, 1141
EcoRV GATATC 3 cut(s) 97, 664, 1448
EcoT14I CCWWGG 2 cut(s) 224, 513
ErhI CCWWGG 2 cut(s) 224, 513
FaeI CATG 5 cut(s) 103, 228, 642, 797, 1438
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FaqI GGGAC 2 cut(s) 264, 1352
FatI CATG 5 cut(s) 99, 224, 638, 793, 1434
FbaI TGATCA 1 cut(s) 1051
FblI GTMKAC 1 cut(s) 822
Fnu4HI GCNGC 4 cut(s) 256, 585, 759, 1220
FokI GGATG 5 cut(s) 78, 160, 433, 755, 1100
Fsp4HI GCNGC 4 cut(s) 256, 585, 759, 1220
FspBI CTAG 2 cut(s) 914, 1281
GluI GCNGC 4 cut(s) 256, 585, 759, 1220
GsuI CTGGAG 1 cut(s) 1338
HapII CCGG 1 cut(s) 626
Hin1II CATG 5 cut(s) 103, 228, 642, 797, 1438
HindIII AAGCTT 2 cut(s) 1225, 1407
HinfI GANTC 7 cut(s) 109, 301, 569, 730, 917, 966, 1295
HpaII CCGG 1 cut(s) 626
HphI GGTGA 5 cut(s) 961, 1004, 1034, 1112, 1409
Hpy166II GTNNAC 3 cut(s) 823, 886, 1464
Hpy188I TCNGA 4 cut(s) 141, 289, 568, 1445
Hpy188III TCNNGA 7 cut(s) 426, 472, 727, 1055, 1154, 1355, 1373
Hpy8I GTNNAC 3 cut(s) 823, 886, 1464
Hpy99I CGWCG 2 cut(s) 536, 867
HpyAV CCTTC 5 cut(s) 452, 469, 1052, 1091, 1283
HpyCH4III ACNGT 4 cut(s) 580, 646, 929, 1331
HpyCH4IV ACGT 5 cut(s) 531, 777, 865, 1047, 1431
HpyCH4V TGCA 6 cut(s) 92, 162, 255, 758, 1222, 1322
HpyF10VI GCNNNNNNNGC 3 cut(s) 85, 159, 322
HpyF3I CTNAG 3 cut(s) 288, 321, 658
HpySE526I ACGT 5 cut(s) 531, 777, 865, 1047, 1431
Hsp92II CATG 5 cut(s) 103, 228, 642, 797, 1438
Ksp22I TGATCA 1 cut(s) 1051
Kzo9I GATC 8 cut(s) 22, 43, 136, 195, 790, 942, 1012, 1051
LmnI GCTCC 2 cut(s) 214, 321
Lsp1109I GCAGC 4 cut(s) 267, 596, 770, 1206
LweI GCATC 2 cut(s) 745, 756
MaeI CTAG 2 cut(s) 914, 1281
MaeII ACGT 5 cut(s) 531, 777, 865, 1047, 1431
MaeIII GTNAC 3 cut(s) 228, 527, 646
MalI GATC 8 cut(s) 24, 45, 138, 197, 792, 944, 1014, 1053
MboI GATC 8 cut(s) 22, 43, 136, 195, 790, 942, 1012, 1051
MflI RGATCY 2 cut(s) 43, 195
MluCI AATT 9 cut(s) 177, 307, 420, 459, 518, 803, 852, 1157, 1171
MlyI GAGTC 2 cut(s) 310, 739
MmeI TCCRAC 1 cut(s) 936
MseI TTAA 8 cut(s) 558, 594, 773, 855, 1128, 1149, 1160, 1389
MslI CAYNNNNRTG 1 cut(s) 1020
MspI CCGG 1 cut(s) 626
MspR9I CCNGG 3 cut(s) 490, 627, 1143
MvaI CCWGG 2 cut(s) 490, 1143
MvnI CGCG 1 cut(s) 536
MwoI GCNNNNNNNGC 3 cut(s) 85, 159, 322
NciI CCSGG 1 cut(s) 627
NcoI CCATGG 1 cut(s) 224
NdeII GATC 8 cut(s) 22, 43, 136, 195, 790, 942, 1012, 1051
NlaIII CATG 5 cut(s) 103, 228, 642, 797, 1438
NlaIV GGNNCC 2 cut(s) 280, 511
NmuCI GTSAC 2 cut(s) 527, 646
PfeI GAWTC 5 cut(s) 109, 569, 917, 966, 1295
PflMI CCANNNNNTGG 1 cut(s) 495
PkrI GCNGC 4 cut(s) 257, 586, 760, 1221
Ple19I CGATCG 1 cut(s) 139
PleI GAGTC 2 cut(s) 309, 738
PpsI GAGTC 2 cut(s) 309, 738
PshBI ATTAAT 2 cut(s) 855, 1128
PsiI TTATAA 1 cut(s) 57
Psp1406I AACGTT 1 cut(s) 777
Psp6I CCWGG 2 cut(s) 488, 1141
PspGI CCWGG 2 cut(s) 488, 1141
PspN4I GGNNCC 2 cut(s) 280, 511
PspPI GGNCC 1 cut(s) 278
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PstI CTGCAG 2 cut(s) 94, 257
PstNI CAGNNNCTG 1 cut(s) 1335
PsuI RGATCY 2 cut(s) 43, 195
PvuI CGATCG 1 cut(s) 139
RsaI GTAC 2 cut(s) 432, 899
RsaNI GTAC 2 cut(s) 431, 898
RseI CAYNNNNRTG 1 cut(s) 1020
SaqAI TTAA 8 cut(s) 558, 594, 773, 855, 1128, 1149, 1160, 1389
SatI GCNGC 4 cut(s) 256, 585, 759, 1220
Sau3AI GATC 8 cut(s) 22, 43, 136, 195, 790, 942, 1012, 1051
Sau96I GGNCC 1 cut(s) 278
ScaI AGTACT 1 cut(s) 432
SchI GAGTC 2 cut(s) 310, 739
ScrFI CCNGG 3 cut(s) 490, 627, 1143
SfaNI GCATC 2 cut(s) 745, 756
SfcI CTRYAG 2 cut(s) 90, 253
SinI GGWCC 1 cut(s) 278
SmiMI CAYNNNNRTG 1 cut(s) 1020
SmlI CTYRAG 3 cut(s) 240, 451, 651
SmoI CTYRAG 3 cut(s) 240, 451, 651
Sse9I AATT 9 cut(s) 177, 307, 420, 459, 518, 803, 852, 1157, 1171
SsiI CCGC 4 cut(s) 30, 86, 797, 1251
SspI AATATT 2 cut(s) 1033, 1242
SspMI CTAG 2 cut(s) 914, 1281
StyD4I CCNGG 3 cut(s) 488, 625, 1141
StyI CCWWGG 2 cut(s) 224, 513
TaaI ACNGT 4 cut(s) 580, 646, 929, 1331
TaiI ACGT 5 cut(s) 534, 780, 868, 1050, 1434
TaqI TCGA 1 cut(s) 331
TaqII GACCGA 1 cut(s) 266
TasI AATT 9 cut(s) 177, 307, 420, 459, 518, 803, 852, 1157, 1171
TatI WGTACW 2 cut(s) 430, 897
TfiI GAWTC 5 cut(s) 109, 569, 917, 966, 1295
Tru1I TTAA 8 cut(s) 558, 594, 773, 855, 1128, 1149, 1160, 1389
Tru9I TTAA 8 cut(s) 558, 594, 773, 855, 1128, 1149, 1160, 1389
TscAI CASTG 5 cut(s) 211, 257, 585, 934, 1336
TseFI GTSAC 2 cut(s) 527, 646
TseI GCWGC 4 cut(s) 255, 584, 758, 1219
Tsp45I GTSAC 2 cut(s) 527, 646
TspGWI ACGGA 1 cut(s) 160
TspRI CASTG 5 cut(s) 211, 257, 585, 934, 1336
Van91I CCANNNNNTGG 1 cut(s) 495
VpaK11BI GGWCC 1 cut(s) 278
VspI ATTAAT 2 cut(s) 855, 1128
XapI RAATTY 3 cut(s) 518, 803, 1171
XmiI GTMKAC 1 cut(s) 822
XspI CTAG 2 cut(s) 914, 1281
ZrmI AGTACT 1 cut(s) 432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.