Rroxscaffold_178G00437520

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000178
OG0000144
Physical Location & Seq
Forward (+)
240512 .. 244237
3726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_178G00437520.1

Sequence Viewer

Length: 1683 bp
ATGAAGAAACAACAACTGACCTTGAAGCTAAGAAACTCATTAGTCCCTTGTGCACATACCAGTATGTGTTCAACGCTAGTGACTAAGGTTCTCAATGACATGGATGTCCCCGAATATGAGCAGGAAAGAATTTCTAGGCAAATAACTTGGGACACTACCTCCGCTGGTTTACCAGGTCTGTTTCCTTCAAGCAATTACATGAACACAAAATATCTGCAGGCAAGAGAAAACAAATCGAGAACTGCAACGACGACCAAATTACTTGTCATGGGTCAGTTGGGTGCTGGCAGACACCGCCACAAGACATCATGGAGGGAGATTCTACAGAAAATGGGTTTAATTGCTCGTACACGCTTAGCCAAGGTCTGCATGTCTTGGAGTTCACTTGTTATGAGAGCTGATATCCGCAGTGCTCCGCAACTGCCATGGTTAGTACACCCTCAAACCCCAAACACCAACTACTTAACCTTTTCCAGCCTCTCTGAGGGTGAAGTTGTGAACTTGGAGCTCCCAAAGCGATTCCGAGGACAAGGCAAGTTCAAGTTTCGAGCGTCTTCTAAAGGTTGGTTGATCATTTTACAAGAAAAAGGATTCGGCTTTGATATGTTCCTATACAATCCGATTTCTAGAGCCCAACACCAACTTCCTTCCTTAAACACTATTCCATCTTTCCTATCTTTCCAACTAGCAAGAATGCTAGGGAAGGTTATCAACAATCATGCCAACAAACATGCCGAGAATTTTGTATCTAAAGTTGTGTTGTCTAGTCCAAATGTCTCAGAATGCATTGTGGCTGCCGTTTTCGAACCTCAGCATGAATTGGGATTATGCAGACCTGGAGACAAAAGCTGGACTGTCTTTGAGATACTGAATCATGAGGACGACTTCCTCATGGATTTACTTTTTTCGCAGGGCATGCTATACGGCTTTGTTGATAATAGCAAGTCCACCAAGAATGGCATCGGCGCAGCTCGCATCTTAAACTTTGGAGATGATCAGGCAATGACAGTGGAATTGAAGTTGGTCTACGGCCATAAAGAAGTTGGTAATACTACAGAATATCATGATGGCTTTCTAATCTTTTTAAATAGAGACTACAACTCATATTTGTTAGAATCAACAACAAATAGCGACAAGGAAATCTTGTTGATCCATCAAATGCTCGATTGTTTAATCCCCAACTGTGATGATGATGATGATGATGATGATGATGATGAGTCGGAAATCGAATATCAGAGGACAAGCGGGTTTGCAGTATACAAGATTGACCTCGAGTATGGCAACTTTCATGAGGTACAAAATTTGGGGGACCAAATAATATTTTTGGCAGAACATAGCTCTGCCTTGTCCTTTCCTGCAAGTGATTTCAACTTACAAGGAAATTGCATTTATTTTGCAACAAGCGGATACGCAACTATCAACCTACCTCGTAAGACCCTTCTTGGATCAAATATATCTCGTGAAATCGGAATCTTTAGTTTAGATGACGGAAAAATTAAGCGACCGTTTCCAAGTCTTGATGGCATGCTATACGGCTTAGTTGATAATGACGAGTCCACCAAGAATGGCATCGGCGCAGCTCGTATCTTAAACTTTGGAGATGATCAGACAATGACAGTGGAATTGAAGTTGGTCTATGGCCATAAAGAAGTTGGTAATACTACAGAAGGTCATGATGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

560

Amino Acids

63.08

Weight (kDa)

5.7

Isoelectric Point (pI)

33.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 157 - 492 3.9e-38 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 104
AccI GTMKAC 2 cut(s) 1026, 1257
AciI CCGC 6 cut(s) 162, 295, 406, 416, 1245, 1404
AclWI GGATC 2 cut(s) 1144, 1453
AcoI YGGCCR 2 cut(s) 1030, 1639
AcsI RAATTY 3 cut(s) 129, 739, 1300
AfaI GTAC 3 cut(s) 349, 435, 1296
AfiI CCNNNNNNNGG 1 cut(s) 484
AgsI TTSAA 7 cut(s) 25, 72, 189, 541, 1018, 1369, 1627
AjnI CCWGG 2 cut(s) 172, 835
AjuI GAANNNNNNNTTGG 2 cut(s) 627, 659
AluBI AGCT 7 cut(s) 28, 398, 508, 849, 971, 1338, 1580
AluI AGCT 7 cut(s) 28, 398, 508, 849, 971, 1338, 1580
Alw21I GWGCWC 3 cut(s) 55, 415, 510
Alw26I GTCTC 3 cut(s) 781, 834, 1086
Alw44I GTGCAC 1 cut(s) 51
AlwI GGATC 2 cut(s) 1144, 1453
Ama87I CYCGRG 1 cut(s) 1271
AoxI GGCC 2 cut(s) 1030, 1639
ApaLI GTGCAC 1 cut(s) 51
ApeKI GCWGC 3 cut(s) 794, 968, 1577
ApoI RAATTY 3 cut(s) 129, 739, 1300
ArsI GACNNNNNNTTYG 2 cut(s) 249, 281
AspLEI GCGC 2 cut(s) 968, 1577
AspS9I GGNCC 1 cut(s) 1309
AsuHPI GGTGA 1 cut(s) 500
AsuII TTCGAA 1 cut(s) 804
AvaI CYCGRG 1 cut(s) 1271
AvaII GGWCC 1 cut(s) 1309
BaeGI GKGCMC 1 cut(s) 55
BalI TGGCCA 1 cut(s) 1641
BanII GRGCYC 2 cut(s) 510, 634
BarI GAAGNNNNNNTAC 2 cut(s) 1010, 1042
BauI CACGAG 1 cut(s) 1458
BbsI GAAGAC 1 cut(s) 546
Bbv12I GWGCWC 3 cut(s) 55, 415, 510
BbvCI CCTCAGC 1 cut(s) 810
BbvI GCAGC 3 cut(s) 781, 980, 1589
BccI CCATC 4 cut(s) 673, 1061, 1161, 1514
BceAI ACGGC 4 cut(s) 782, 940, 1045, 1549
BciT130I CCWGG 2 cut(s) 174, 837
BciVI GTATCC 1 cut(s) 1400
BclI TGATCA 3 cut(s) 570, 994, 1603
BcoDI GTCTC 3 cut(s) 781, 834, 1086
BfaI CTAG 6 cut(s) 77, 135, 627, 686, 698, 765
BfmI CTRYAG 4 cut(s) 215, 323, 1053, 1662
BfuI GTATCC 1 cut(s) 1400
BisI GCNGC 3 cut(s) 795, 969, 1578
BlpI GCTNAGC 1 cut(s) 355
BlsI GCNGC 3 cut(s) 796, 970, 1579
Bme1390I CCNGG 2 cut(s) 174, 837
Bme18I GGWCC 1 cut(s) 1309
BmeT110I CYCGRG 1 cut(s) 1271
BmgT120I GGNCC 1 cut(s) 1309
BmiI GGNNCC 1 cut(s) 1310
BmrFI CCNGG 2 cut(s) 174, 837
BmsI GCATC 3 cut(s) 969, 984, 1578
BpiI GAAGAC 1 cut(s) 546
BpmI CTGGAG 1 cut(s) 858
Bpu10I CCTNAGC 1 cut(s) 810
Bpu1102I GCTNAGC 1 cut(s) 355
Bpu14I TTCGAA 1 cut(s) 804
BsaJI CCNNGG 3 cut(s) 360, 425, 523
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 1 cut(s) 484
Bse1I ACTGG 1 cut(s) 60
Bse3DI GCAATG 1 cut(s) 1008
BseBI CCWGG 2 cut(s) 174, 837
BseDI CCNNGG 3 cut(s) 360, 425, 523
BseGI GGATG 1 cut(s) 109
BseLI CCNNNNNNNGG 1 cut(s) 484
BseMI GCAATG 1 cut(s) 1008
BseMII CTCAG 3 cut(s) 474, 792, 824
BseNI ACTGG 1 cut(s) 60
BseSI GKGCMC 1 cut(s) 55
BseXI GCAGC 3 cut(s) 781, 980, 1589
Bsh1285I CGRYCG 1 cut(s) 1505
BshFI GGCC 2 cut(s) 1032, 1641
BsiEI CGRYCG 1 cut(s) 1505
BsiHKAI GWGCWC 3 cut(s) 55, 415, 510
BsiHKCI CYCGRG 1 cut(s) 1271
BslFI GGGAC 4 cut(s) 29, 92, 164, 1322
BslI CCNNNNNNNGG 1 cut(s) 484
BsmAI GTCTC 3 cut(s) 781, 834, 1086
BsmFI GGGAC 4 cut(s) 29, 92, 164, 1322
BsmI GAATGC 2 cut(s) 699, 788
BsnI GGCC 2 cut(s) 1032, 1641
BsoBI CYCGRG 1 cut(s) 1271
Bsp119I TTCGAA 1 cut(s) 804
Bsp1286I GDGCHC 4 cut(s) 55, 415, 510, 634
Bsp143I GATC 5 cut(s) 570, 994, 1149, 1445, 1603
Bsp1720I GCTNAGC 1 cut(s) 355
Bsp19I CCATGG 1 cut(s) 425
BspACI CCGC 6 cut(s) 162, 295, 406, 416, 1245, 1404
BspANI GGCC 2 cut(s) 1032, 1641
BspCNI CTCAG 3 cut(s) 475, 791, 823
BspHI TCATGA 4 cut(s) 874, 1063, 1288, 1672
BspLI GGNNCC 1 cut(s) 1310
BspMAI CTGCAG 1 cut(s) 219
BspPI GGATC 2 cut(s) 1144, 1453
BspT104I TTCGAA 1 cut(s) 804
BsrDI GCAATG 1 cut(s) 1008
BsrI ACTGG 1 cut(s) 60
BssECI CCNNGG 3 cut(s) 360, 425, 523
BssMI GATC 5 cut(s) 570, 994, 1149, 1445, 1603
BssNAI GTATAC 1 cut(s) 1258
BssSI CACGAG 1 cut(s) 1458
BssT1I CCWWGG 2 cut(s) 360, 425
Bst1107I GTATAC 1 cut(s) 1258
Bst2BI CACGAG 1 cut(s) 1458
Bst2UI CCWGG 2 cut(s) 174, 837
Bst4CI ACNGT 5 cut(s) 856, 1009, 1184, 1506, 1618
BstAPI GCANNNNNTGC 1 cut(s) 916
BstBI TTCGAA 1 cut(s) 804
BstC8I GCNNGC 5 cut(s) 219, 286, 917, 973, 1526
BstDEI CTNAG 7 cut(s) 29, 84, 355, 483, 778, 810, 1537
BstDSI CCRYGG 1 cut(s) 425
BstENI CCTNNNNNAGG 1 cut(s) 482
BstF5I GGATG 1 cut(s) 109
BstHHI GCGC 2 cut(s) 968, 1577
BstKTI GATC 5 cut(s) 573, 997, 1152, 1448, 1606
BstMAI GTCTC 3 cut(s) 781, 834, 1086
BstMBI GATC 5 cut(s) 570, 994, 1149, 1445, 1603
BstMCI CGRYCG 1 cut(s) 1505
BstMWI GCNNNNNNNGC 4 cut(s) 294, 514, 916, 972
BstNI CCWGG 2 cut(s) 174, 837
BstNSI RCATGY 4 cut(s) 373, 734, 919, 1528
BstSCI CCNGG 2 cut(s) 172, 835
BstSFI CTRYAG 4 cut(s) 215, 323, 1053, 1662
BstSLI GKGCMC 1 cut(s) 55
BstV1I GCAGC 3 cut(s) 781, 980, 1589
BstV2I GAAGAC 1 cut(s) 546
BstZ17I GTATAC 1 cut(s) 1258
BsuI GTATCC 1 cut(s) 1400
BsuRI GGCC 2 cut(s) 1032, 1641
BtgI CCRYGG 1 cut(s) 425
BtsCI GGATG 1 cut(s) 109
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 3 cut(s) 415, 1014, 1623
Cac8I GCNNGC 5 cut(s) 219, 286, 917, 973, 1526
CciI TCATGA 4 cut(s) 874, 1063, 1288, 1672
CfoI GCGC 2 cut(s) 968, 1577
Cfr13I GGNCC 1 cut(s) 1309
CseI GACGC 1 cut(s) 540
CsiI ACCWGGT 1 cut(s) 172
Csp6I GTAC 3 cut(s) 348, 434, 1295
CspCI CAANNNNNGTGG 4 cut(s) 990, 1025, 1599, 1634
CviQI GTAC 3 cut(s) 348, 434, 1295
DdeI CTNAG 7 cut(s) 29, 84, 355, 483, 778, 810, 1537
DpnI GATC 5 cut(s) 572, 996, 1151, 1447, 1605
DpnII GATC 5 cut(s) 570, 994, 1149, 1445, 1603
DraI TTTAAA 1 cut(s) 1086
DrdI GACNNNNNNGTC 1 cut(s) 104
DseDI GACNNNNNNGTC 1 cut(s) 104
EaeI YGGCCR 2 cut(s) 1030, 1639
Ecl136II GAGCTC 1 cut(s) 508
Eco130I CCWWGG 2 cut(s) 360, 425
Eco24I GRGCYC 2 cut(s) 510, 634
Eco32I GATATC 1 cut(s) 403
Eco47I GGWCC 1 cut(s) 1309
Eco53kI GAGCTC 1 cut(s) 508
Eco88I CYCGRG 1 cut(s) 1271
EcoICRI GAGCTC 1 cut(s) 508
EcoNI CCTNNNNNAGG 1 cut(s) 482
EcoRII CCWGG 2 cut(s) 172, 835
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 2 cut(s) 360, 425
EcoT22I ATGCAT 1 cut(s) 788
EcoT38I GRGCYC 2 cut(s) 510, 634
ErhI CCWWGG 2 cut(s) 360, 425
FalI AAGNNNNNCTT 2 cut(s) 1127, 1159
FaqI GGGAC 4 cut(s) 29, 92, 164, 1322
FauI CCCGC 1 cut(s) 1238
FbaI TGATCA 3 cut(s) 570, 994, 1603
FblI GTMKAC 2 cut(s) 1026, 1257
Fnu4HI GCNGC 3 cut(s) 795, 969, 1578
FokI GGATG 1 cut(s) 116
FriOI GRGCYC 2 cut(s) 510, 634
Fsp4HI GCNGC 3 cut(s) 795, 969, 1578
FspBI CTAG 6 cut(s) 77, 135, 627, 686, 698, 765
GlaI GCGC 2 cut(s) 967, 1576
GluI GCNGC 3 cut(s) 795, 969, 1578
GsuI CTGGAG 1 cut(s) 858
HaeIII GGCC 2 cut(s) 1032, 1641
HgaI GACGC 1 cut(s) 540
HhaI GCGC 2 cut(s) 968, 1577
Hin6I GCGC 2 cut(s) 966, 1575
HinP1I GCGC 2 cut(s) 966, 1575
HinfI GANTC 8 cut(s) 319, 519, 591, 871, 1115, 1217, 1470, 1553
HphI GGTGA 1 cut(s) 500
Hpy188I TCNGA 8 cut(s) 484, 524, 621, 781, 1222, 1236, 1469, 1608
Hpy188III TCNNGA 8 cut(s) 237, 627, 875, 1064, 1289, 1460, 1517, 1673
Hpy99I CGWCG 1 cut(s) 253
HpyAV CCTTC 5 cut(s) 195, 657, 697, 1448, 1661
HpyCH4III ACNGT 5 cut(s) 856, 1009, 1184, 1506, 1618
HpyF10VI GCNNNNNNNGC 4 cut(s) 294, 514, 916, 972
HpyF3I CTNAG 7 cut(s) 29, 84, 355, 483, 778, 810, 1537
HspAI GCGC 2 cut(s) 966, 1575
Ksp22I TGATCA 3 cut(s) 570, 994, 1603
Kzo9I GATC 5 cut(s) 570, 994, 1149, 1445, 1603
LmnI GCTCC 3 cut(s) 418, 505, 513
Lsp1109I GCAGC 3 cut(s) 781, 980, 1589
LweI GCATC 3 cut(s) 969, 984, 1578
MabI ACCWGGT 1 cut(s) 172
MaeI CTAG 6 cut(s) 77, 135, 627, 686, 698, 765
MaeIII GTNAC 1 cut(s) 79
MalI GATC 5 cut(s) 572, 996, 1151, 1447, 1605
MboI GATC 5 cut(s) 570, 994, 1149, 1445, 1603
MboII GAAGA 2 cut(s) 16, 546
MhlI GDGCHC 4 cut(s) 55, 415, 510, 634
MlsI TGGCCA 1 cut(s) 1641
MluNI TGGCCA 1 cut(s) 1641
MlyI GAGTC 2 cut(s) 1226, 1562
MmeI TCCRAC 2 cut(s) 706, 1200
Mox20I TGGCCA 1 cut(s) 1641
Mph1103I ATGCAT 1 cut(s) 788
MscI TGGCCA 1 cut(s) 1641
MseI TTAA 8 cut(s) 338, 464, 653, 980, 1085, 1172, 1497, 1589
Msp20I TGGCCA 1 cut(s) 1641
MspA1I CMGCKG 1 cut(s) 164
MspR9I CCNGG 2 cut(s) 174, 837
Mva1269I GAATGC 2 cut(s) 699, 788
MvaI CCWGG 2 cut(s) 174, 837
MwoI GCNNNNNNNGC 4 cut(s) 294, 514, 916, 972
NcoI CCATGG 1 cut(s) 425
NdeII GATC 5 cut(s) 570, 994, 1149, 1445, 1603
NlaIV GGNNCC 1 cut(s) 1310
NmeAIII GCCGAG 1 cut(s) 760
NmuCI GTSAC 1 cut(s) 79
NsiI ATGCAT 1 cut(s) 788
NspI RCATGY 4 cut(s) 373, 734, 919, 1528
NspV TTCGAA 1 cut(s) 804
PaeI GCATGC 2 cut(s) 919, 1528
PaeR7I CTCGAG 1 cut(s) 1271
PagI TCATGA 4 cut(s) 874, 1063, 1288, 1672
PctI GAATGC 2 cut(s) 699, 788
PfeI GAWTC 6 cut(s) 319, 519, 591, 871, 1115, 1470
PkrI GCNGC 3 cut(s) 796, 970, 1579
PleI GAGTC 2 cut(s) 1225, 1561
PpsI GAGTC 2 cut(s) 1225, 1561
Psp124BI GAGCTC 1 cut(s) 510
Psp6I CCWGG 2 cut(s) 172, 835
PspGI CCWGG 2 cut(s) 172, 835
PspN4I GGNNCC 1 cut(s) 1310
PspPI GGNCC 1 cut(s) 1309
PspXI VCTCGAGB 1 cut(s) 1271
PstI CTGCAG 1 cut(s) 219
RsaI GTAC 3 cut(s) 349, 435, 1296
RsaNI GTAC 3 cut(s) 348, 434, 1295
SacI GAGCTC 1 cut(s) 510
SaqAI TTAA 8 cut(s) 338, 464, 653, 980, 1085, 1172, 1497, 1589
SatI GCNGC 3 cut(s) 795, 969, 1578
Sau3AI GATC 5 cut(s) 570, 994, 1149, 1445, 1603
Sau96I GGNCC 1 cut(s) 1309
SchI GAGTC 2 cut(s) 1226, 1562
ScrFI CCNGG 2 cut(s) 174, 837
SduI GDGCHC 4 cut(s) 55, 415, 510, 634
SexAI ACCWGGT 1 cut(s) 172
SfaNI GCATC 3 cut(s) 969, 984, 1578
SfcI CTRYAG 4 cut(s) 215, 323, 1053, 1662
Sfr274I CTCGAG 1 cut(s) 1271
SfuI TTCGAA 1 cut(s) 804
SinI GGWCC 1 cut(s) 1309
SlaI CTCGAG 1 cut(s) 1271
SmlI CTYRAG 1 cut(s) 1271
SmoI CTYRAG 1 cut(s) 1271
SphI GCATGC 2 cut(s) 919, 1528
SsiI CCGC 6 cut(s) 162, 295, 406, 416, 1245, 1404
SspI AATATT 1 cut(s) 1320
SspMI CTAG 6 cut(s) 77, 135, 627, 686, 698, 765
SstI GAGCTC 1 cut(s) 510
StyD4I CCNGG 2 cut(s) 172, 835
StyI CCWWGG 2 cut(s) 360, 425
TaaI ACNGT 5 cut(s) 856, 1009, 1184, 1506, 1618
TaqI TCGA 6 cut(s) 236, 547, 804, 1164, 1227, 1272
TatI WGTACW 1 cut(s) 433
TfiI GAWTC 6 cut(s) 319, 519, 591, 871, 1115, 1470
Tru1I TTAA 8 cut(s) 338, 464, 653, 980, 1085, 1172, 1497, 1589
Tru9I TTAA 8 cut(s) 338, 464, 653, 980, 1085, 1172, 1497, 1589
TscAI CASTG 3 cut(s) 415, 1014, 1623
TseFI GTSAC 1 cut(s) 79
TseI GCWGC 3 cut(s) 794, 968, 1577
Tsp45I GTSAC 1 cut(s) 79
TspDTI ATGAA 4 cut(s) 17, 215, 831, 1277
TspGWI ACGGA 1 cut(s) 1503
TspRI CASTG 3 cut(s) 415, 1014, 1623
VneI GTGCAC 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 1309
XagI CCTNNNNNAGG 1 cut(s) 482
XapI RAATTY 3 cut(s) 129, 739, 1300
XbaI TCTAGA 1 cut(s) 626
XceI RCATGY 4 cut(s) 373, 734, 919, 1528
XcmI CCANNNNNNNNNTGG 2 cut(s) 1040, 1649
XhoI CTCGAG 1 cut(s) 1271
XmiI GTMKAC 2 cut(s) 1026, 1257
XspI CTAG 6 cut(s) 77, 135, 627, 686, 698, 765
Zsp2I ATGCAT 1 cut(s) 788
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.