Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB7I | CCANNNNNTGG | 1 cut(s) | 222 |
| AclWI | GGATC | 1 cut(s) | 198 |
| AfaI | GTAC | 1 cut(s) | 164 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 222 |
| AflIII | ACRYGT | 1 cut(s) | 515 |
| AgsI | TTSAA | 1 cut(s) | 494 |
| AjnI | CCWGG | 1 cut(s) | 275 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 486, 518 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 384, 416 |
| AluBI | AGCT | 3 cut(s) | 236, 258, 333 |
| AluI | AGCT | 3 cut(s) | 236, 258, 333 |
| AlwI | GGATC | 1 cut(s) | 198 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 142 |
| ApeKI | GCWGC | 2 cut(s) | 5, 373 |
| AspA2I | CCTAGG | 1 cut(s) | 377 |
| AsuHPI | GGTGA | 1 cut(s) | 294 |
| AvrII | CCTAGG | 1 cut(s) | 377 |
| BaeGI | GKGCMC | 1 cut(s) | 513 |
| BbsI | GAAGAC | 1 cut(s) | 402 |
| BbvI | GCAGC | 2 cut(s) | 17, 360 |
| BccI | CCATC | 4 cut(s) | 202, 301, 390, 455 |
| BciT130I | CCWGG | 1 cut(s) | 277 |
| BfaI | CTAG | 1 cut(s) | 378 |
| BisI | GCNGC | 2 cut(s) | 6, 374 |
| BlnI | CCTAGG | 1 cut(s) | 377 |
| BlsI | GCNGC | 2 cut(s) | 7, 375 |
| Bme1390I | CCNGG | 1 cut(s) | 277 |
| BmrFI | CCNGG | 1 cut(s) | 277 |
| BmsI | GCATC | 1 cut(s) | 340 |
| BpiI | GAAGAC | 1 cut(s) | 402 |
| BsaJI | CCNNGG | 2 cut(s) | 275, 377 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 384, 414 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 222 |
| Bse3DI | GCAATG | 1 cut(s) | 52 |
| BseBI | CCWGG | 1 cut(s) | 277 |
| BseDI | CCNNGG | 2 cut(s) | 275, 377 |
| BseGI | GGATG | 2 cut(s) | 213, 401 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 222 |
| BseMI | GCAATG | 1 cut(s) | 52 |
| BseMII | CTCAG | 2 cut(s) | 170, 325 |
| BseRI | GAGGAG | 1 cut(s) | 405 |
| BseSI | GKGCMC | 1 cut(s) | 513 |
| BseXI | GCAGC | 2 cut(s) | 17, 360 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 222 |
| BsmI | GAATGC | 1 cut(s) | 85 |
| Bsp1286I | GDGCHC | 1 cut(s) | 513 |
| Bsp143I | GATC | 4 cut(s) | 9, 75, 108, 190 |
| BspCNI | CTCAG | 2 cut(s) | 171, 324 |
| BspPI | GGATC | 1 cut(s) | 198 |
| BsrDI | GCAATG | 1 cut(s) | 52 |
| BssECI | CCNNGG | 2 cut(s) | 275, 377 |
| BssMI | GATC | 4 cut(s) | 9, 75, 108, 190 |
| BssT1I | CCWWGG | 1 cut(s) | 377 |
| Bst2UI | CCWGG | 1 cut(s) | 277 |
| Bst4CI | ACNGT | 2 cut(s) | 201, 461 |
| Bst6I | CTCTTC | 1 cut(s) | 238 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 142 |
| BstDEI | CTNAG | 2 cut(s) | 179, 311 |
| BstF5I | GGATG | 2 cut(s) | 213, 401 |
| BstKTI | GATC | 4 cut(s) | 12, 78, 111, 193 |
| BstMBI | GATC | 4 cut(s) | 9, 75, 108, 190 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 142, 339 |
| BstNI | CCWGG | 1 cut(s) | 277 |
| BstNSI | RCATGY | 1 cut(s) | 519 |
| BstSCI | CCNGG | 1 cut(s) | 275 |
| BstSLI | GKGCMC | 1 cut(s) | 513 |
| BstV1I | GCAGC | 2 cut(s) | 17, 360 |
| BstV2I | GAAGAC | 1 cut(s) | 402 |
| BtsCI | GGATG | 2 cut(s) | 213, 401 |
| BtsI | GCAGTG | 1 cut(s) | 140 |
| BtsIMutI | CAGTG | 1 cut(s) | 140 |
| CaiI | CAGNNNCTG | 1 cut(s) | 142 |
| CseI | GACGC | 1 cut(s) | 379 |
| Csp6I | GTAC | 1 cut(s) | 163 |
| CviAII | CATG | 3 cut(s) | 157, 404, 516 |
| CviJI | RGCY | 4 cut(s) | 236, 258, 333, 342 |
| CviKI_1 | RGCY | 4 cut(s) | 236, 258, 333, 342 |
| CviQI | GTAC | 1 cut(s) | 163 |
| DdeI | CTNAG | 2 cut(s) | 179, 311 |
| DpnI | GATC | 4 cut(s) | 11, 77, 110, 192 |
| DpnII | GATC | 4 cut(s) | 9, 75, 108, 190 |
| Eam1104I | CTCTTC | 1 cut(s) | 238 |
| EarI | CTCTTC | 1 cut(s) | 238 |
| Eco130I | CCWWGG | 1 cut(s) | 377 |
| Eco32I | GATATC | 1 cut(s) | 154 |
| EcoRII | CCWGG | 1 cut(s) | 275 |
| EcoRV | GATATC | 1 cut(s) | 154 |
| EcoT14I | CCWWGG | 1 cut(s) | 377 |
| ErhI | CCWWGG | 1 cut(s) | 377 |
| FaeI | CATG | 3 cut(s) | 160, 407, 519 |
| FaiI | YATR | 9 cut(s) | 41, 158, 187, 239, 329, 405, 431, 470, 517 |
| FatI | CATG | 3 cut(s) | 156, 403, 515 |
| Fnu4HI | GCNGC | 2 cut(s) | 6, 374 |
| FokI | GGATG | 2 cut(s) | 220, 408 |
| Fsp4HI | GCNGC | 2 cut(s) | 6, 374 |
| FspBI | CTAG | 1 cut(s) | 378 |
| GluI | GCNGC | 2 cut(s) | 6, 374 |
| HgaI | GACGC | 1 cut(s) | 379 |
| Hin1II | CATG | 3 cut(s) | 160, 407, 519 |
| HindIII | AAGCTT | 1 cut(s) | 331 |
| HinfI | GANTC | 1 cut(s) | 299 |
| HphI | GGTGA | 1 cut(s) | 294 |
| Hpy166II | GTNNAC | 1 cut(s) | 361 |
| Hpy188I | TCNGA | 3 cut(s) | 180, 298, 349 |
| Hpy8I | GTNNAC | 1 cut(s) | 361 |
| HpyCH4III | ACNGT | 2 cut(s) | 201, 461 |
| HpyCH4IV | ACGT | 1 cut(s) | 175 |
| HpyCH4V | TGCA | 1 cut(s) | 129 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 142, 339 |
| HpyF3I | CTNAG | 2 cut(s) | 179, 311 |
| HpySE526I | ACGT | 1 cut(s) | 175 |
| Hsp92II | CATG | 3 cut(s) | 160, 407, 519 |
| Kzo9I | GATC | 4 cut(s) | 9, 75, 108, 190 |
| LpnPI | CCDG | 3 cut(s) | 159, 262, 289 |
| Lsp1109I | GCAGC | 2 cut(s) | 17, 360 |
| LweI | GCATC | 1 cut(s) | 340 |
| MaeI | CTAG | 1 cut(s) | 378 |
| MaeII | ACGT | 1 cut(s) | 175 |
| MaeIII | GTNAC | 1 cut(s) | 279 |
| MalI | GATC | 4 cut(s) | 11, 77, 110, 192 |
| MboI | GATC | 4 cut(s) | 9, 75, 108, 190 |
| MboII | GAAGA | 4 cut(s) | 82, 85, 255, 402 |
| MhlI | GDGCHC | 1 cut(s) | 513 |
| MluCI | AATT | 2 cut(s) | 478, 527 |
| MmeI | TCCRAC | 2 cut(s) | 287, 484 |
| MnlI | CCTC | 5 cut(s) | 219, 300, 383, 386, 500 |
| MseI | TTAA | 2 cut(s) | 203, 453 |
| MspR9I | CCNGG | 1 cut(s) | 277 |
| Mva1269I | GAATGC | 1 cut(s) | 85 |
| MvaI | CCWGG | 1 cut(s) | 277 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 142, 339 |
| NdeII | GATC | 4 cut(s) | 9, 75, 108, 190 |
| NlaIII | CATG | 3 cut(s) | 160, 407, 519 |
| NspI | RCATGY | 1 cut(s) | 519 |
| PciI | ACATGT | 1 cut(s) | 515 |
| PctI | GAATGC | 1 cut(s) | 85 |
| PfeI | GAWTC | 1 cut(s) | 299 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 222 |
| PkrI | GCNGC | 2 cut(s) | 7, 375 |
| PscI | ACATGT | 1 cut(s) | 515 |
| Psp6I | CCWGG | 1 cut(s) | 275 |
| PspGI | CCWGG | 1 cut(s) | 275 |
| PstNI | CAGNNNCTG | 1 cut(s) | 142 |
| RsaI | GTAC | 1 cut(s) | 164 |
| RsaNI | GTAC | 1 cut(s) | 163 |
| SaqAI | TTAA | 2 cut(s) | 203, 453 |
| SatI | GCNGC | 2 cut(s) | 6, 374 |
| Sau3AI | GATC | 4 cut(s) | 9, 75, 108, 190 |
| ScrFI | CCNGG | 1 cut(s) | 277 |
| SduI | GDGCHC | 1 cut(s) | 513 |
| SetI | ASST | 6 cut(s) | 178, 230, 238, 260, 335, 421 |
| SfaNI | GCATC | 1 cut(s) | 340 |
| Sse9I | AATT | 2 cut(s) | 478, 527 |
| SspMI | CTAG | 1 cut(s) | 378 |
| StyD4I | CCNGG | 1 cut(s) | 275 |
| StyI | CCWWGG | 1 cut(s) | 377 |
| TaaI | ACNGT | 2 cut(s) | 201, 461 |
| TaiI | ACGT | 1 cut(s) | 178 |
| TaqI | TCGA | 1 cut(s) | 193 |
| TasI | AATT | 2 cut(s) | 478, 527 |
| TatI | WGTACW | 1 cut(s) | 162 |
| TfiI | GAWTC | 1 cut(s) | 299 |
| Tru1I | TTAA | 2 cut(s) | 203, 453 |
| Tru9I | TTAA | 2 cut(s) | 203, 453 |
| TscAI | CASTG | 1 cut(s) | 147 |
| TseI | GCWGC | 2 cut(s) | 5, 373 |
| TspDTI | ATGAA | 1 cut(s) | 392 |
| TspRI | CASTG | 1 cut(s) | 147 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 222 |
| XceI | RCATGY | 1 cut(s) | 519 |
| XmaJI | CCTAGG | 1 cut(s) | 377 |
| XspI | CTAG | 1 cut(s) | 378 |