Rorug05G0554300

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000144
Physical Location & Seq
Forward (+)
74189688 .. 74192725
3038 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0554300.1

Sequence Viewer

Length: 1164 bp
ATGGAAGAAAGAGGATGCGAGCCTGACATATTTACCTATAGCACCATCATTCACAGTCTTTGCAAGGACACACTTGTTGTTGATGCAATGAACCTCTTCTCAGAAATGATAAGTAAAGGTATTGCTCCAAACGTTGTTACTTACACCTCTCTAATTCATGGAGTTTGCAATTTAGGACAGTGGAAAGAAGCTACAAGGTTTTTGAATGAAATGGTGAGTAAAGGTATCTTTCCAAATGTCTTCACTTTCAATGTTTTGGTTGATACACTGTGTAAGGAAGGGATGGTTGTGGAAGCTGAAAGTGTGCTTGACATGATGATTCAAAGACATATTCAGCCTGATATGATTACATACAGTTCCCTTATGGATGGTTACTGTTTGCGAGGAGAAATGGACAAGGCGAAACAAGTTTTTGATCTAATGGTTAGCAAGGGCTCCATGGTTAATGTTCAGAGTTGTAGCATATTGATAAACGGATACTGTAAGCAGAAAAAGATCGATGAGGCCAATAAGGTTTTTCAGGAAATGACTTGTTGGGAGCTTGTTCCAAACACCGTTACTTTTAACACTCTCATTGATGGTTTTTACAAAGCAGGGAGAATACAAGATGCAGAAAAGTTGTTCTCTGAGATGCAAACTTGTGGCCAACTTCCAGATGTTCAAACTTACACTATTATACTTGATGCCCTATTTAGCAACAAACAACTTTCTAAAGCAATAGAATTGCTTAGAGAGATGGAAGACAAGAAGTTGGAACTAAATATTGTAGTTTACACCGTTATCATTGAAGGTTTGTTGAAAGCTGGAGAAGTTCAGTCTGCAAAAGATCTCTTCTATAGTTTATTTTCAAAAGGAGTTCAACCTGATGTGTGGACATATAATATAATGATTAGCGGACTTTGCAAAGGAGGCCTATTAAGTGAAGCAGAAAATTTGCTTAGGGGAATGGAACAAAAAGGCTGTTCTCCAAATGATTGTACCTATAACACAATTATCCGAGGTTTTATGAATAACAATGAGATATCAAGGGCTATGGAACTTATTCAAGAAATGCGTGAGAGGGGATTCTCTGCAGATGCATCAACTATGGACTTGATTGTTGATTTATTGTCAAAGGATACAGTAGATCCTGTATTATTAGCATGGCTTAAAGATTCAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

387

Amino Acids

43.59

Weight (kDa)

4.76

Isoelectric Point (pI)

29.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 1 - 22 6e-06 PPR repeat family
PPR_1 PF12854 4 - 36 1.1e-12 PPR repeat
PPR_2 PF13041 8 - 57 2.4e-17 PPR repeat family
PPR PF01535 11 - 41 4.8e-06 PPR repeat
PPR_3 PF13812 32 - 90 2.7e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 39 - 72 7e-12 PPR repeat
PPR_2 PF13041 43 - 92 1.1e-18 PPR repeat family
PPR PF01535 46 - 76 1.7e-07 PPR repeat
MRP-S27 PF10037 47 - 109 1.1e-06 Mitochondrial 28S ribosomal protein S27
PPR_1 PF12854 74 - 106 3.5e-11 PPR repeat
PPR_2 PF13041 80 - 126 5.5e-13 PPR repeat family
PPR PF01535 81 - 110 9.8e-06 PPR repeat
PPR_1 PF12854 110 - 142 6.7e-11 PPR repeat
PPR PF01535 116 - 145 3.8e-08 PPR repeat
PPR_2 PF13041 120 - 162 5.2e-10 PPR repeat family
PPR_3 PF13812 141 - 193 4.1e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 149 - 197 9e-16 PPR repeat family
PPR PF01535 152 - 177 3e-06 PPR repeat
PPR_1 PF12854 154 - 177 4.7e-07 PPR repeat
PPR_long PF17177 162 - 272 2e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 180 - 212 4.4e-12 PPR repeat
PPR_2 PF13041 183 - 230 2e-17 PPR repeat family
TPR_24 PF23276 183 - 279 2.4e-10 Fungal tetratrico peptide repeats
PPR PF01535 186 - 215 1.7e-08 PPR repeat
PPR_3 PF13812 206 - 263 1.7e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 215 - 247 1.2e-06 PPR repeat
PPR_2 PF13041 222 - 265 2.5e-09 PPR repeat family
PPR_3 PF13812 242 - 298 1.2e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 254 - 302 3e-15 PPR repeat family
PPR_3 PF13812 283 - 335 2.9e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 284 - 317 2.6e-13 PPR repeat
PPR PF01535 292 - 321 8.6e-09 PPR repeat
PPR_2 PF13041 292 - 336 1.9e-14 PPR repeat family
PPR_1 PF12854 319 - 352 6.1e-09 PPR repeat
PPR_2 PF13041 326 - 370 1.8e-09 PPR repeat family
PPR PF01535 327 - 356 1e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 894
AclI AACGTT 1 cut(s) 132
AclWI GGATC 1 cut(s) 1121
AcoI YGGCCR 1 cut(s) 643
AcsI RAATTY 1 cut(s) 931
AfaI GTAC 1 cut(s) 979
AgsI TTSAA 9 cut(s) 205, 250, 323, 662, 788, 799, 849, 860, 1046
AluBI AGCT 4 cut(s) 191, 296, 541, 803
AluI AGCT 4 cut(s) 191, 296, 541, 803
AlwI GGATC 1 cut(s) 1121
AoxI GGCC 3 cut(s) 504, 643, 910
ApoI RAATTY 1 cut(s) 931
Asp700I GAANNNNTTC 2 cut(s) 95, 1041
AsuHPI GGTGA 1 cut(s) 226
BalI TGGCCA 1 cut(s) 645
BanII GRGCYC 1 cut(s) 437
BbsI GAAGAC 2 cut(s) 232, 747
BccI CCATC 5 cut(s) 53, 277, 362, 572, 730
BciVI GTATCC 2 cut(s) 470, 1111
BfmI CTRYAG 3 cut(s) 37, 835, 1071
BfuI GTATCC 2 cut(s) 470, 1111
BglII AGATCT 1 cut(s) 826
BmiI GGNNCC 1 cut(s) 436
BmsI GCATC 7 cut(s) 5, 73, 598, 621, 673, 1066, 1088
BpiI GAAGAC 2 cut(s) 232, 747
BpmI CTGGAG 1 cut(s) 825
Bpu10I CCTNAGC 1 cut(s) 938
Bsa29I ATCGAT 1 cut(s) 498
BsaJI CCNNGG 2 cut(s) 438, 997
Bse3DI GCAATG 1 cut(s) 93
BseCI ATCGAT 1 cut(s) 498
BseDI CCNNGG 2 cut(s) 438, 997
BseGI GGATG 3 cut(s) 20, 288, 373
BseMI GCAATG 1 cut(s) 93
BseMII CTCAG 2 cut(s) 114, 618
BseRI GAGGAG 1 cut(s) 399
BshFI GGCC 3 cut(s) 506, 645, 912
BshVI ATCGAT 1 cut(s) 498
BsnI GGCC 3 cut(s) 506, 645, 912
Bsp1286I GDGCHC 1 cut(s) 437
Bsp143I GATC 4 cut(s) 415, 495, 826, 1126
Bsp19I CCATGG 1 cut(s) 438
BspACI CCGC 1 cut(s) 894
BspANI GGCC 3 cut(s) 506, 645, 912
BspCNI CTCAG 2 cut(s) 113, 619
BspDI ATCGAT 1 cut(s) 498
BspLI GGNNCC 1 cut(s) 436
BspMAI CTGCAG 1 cut(s) 1075
BspPI GGATC 1 cut(s) 1121
BsrDI GCAATG 1 cut(s) 93
BssECI CCNNGG 2 cut(s) 438, 997
BssMI GATC 4 cut(s) 415, 495, 826, 1126
BssT1I CCWWGG 1 cut(s) 438
Bst4CI ACNGT 9 cut(s) 56, 180, 270, 356, 377, 482, 556, 778, 1123
Bst6I CTCTTC 2 cut(s) 101, 836
BstC8I GCNNGC 1 cut(s) 20
BstDEI CTNAG 4 cut(s) 100, 627, 728, 938
BstDSI CCRYGG 1 cut(s) 438
BstF5I GGATG 3 cut(s) 20, 288, 373
BstKTI GATC 4 cut(s) 418, 498, 829, 1129
BstMBI GATC 4 cut(s) 415, 495, 826, 1126
BstMWI GCNNNNNNNGC 2 cut(s) 900, 909
BstSFI CTRYAG 3 cut(s) 37, 835, 1071
BstV2I GAAGAC 2 cut(s) 232, 747
BstX2I RGATCY 2 cut(s) 826, 1126
BstYI RGATCY 2 cut(s) 826, 1126
Bsu15I ATCGAT 1 cut(s) 498
BsuI GTATCC 2 cut(s) 470, 1111
BsuRI GGCC 3 cut(s) 506, 645, 912
BsuTUI ATCGAT 1 cut(s) 498
BtgI CCRYGG 1 cut(s) 438
BtsCI GGATG 3 cut(s) 20, 288, 373
BtsIMutI CAGTG 2 cut(s) 185, 266
Cac8I GCNNGC 1 cut(s) 20
ClaI ATCGAT 1 cut(s) 498
Csp6I GTAC 1 cut(s) 978
CviAII CATG 4 cut(s) 158, 313, 439, 1143
CviQI GTAC 1 cut(s) 978
DdeI CTNAG 4 cut(s) 100, 627, 728, 938
DpnI GATC 4 cut(s) 417, 497, 828, 1128
DpnII GATC 4 cut(s) 415, 495, 826, 1126
EaeI YGGCCR 1 cut(s) 643
Eam1104I CTCTTC 2 cut(s) 101, 836
EarI CTCTTC 2 cut(s) 101, 836
Eco130I CCWWGG 1 cut(s) 438
Eco147I AGGCCT 1 cut(s) 912
Eco24I GRGCYC 1 cut(s) 437
Eco32I GATATC 1 cut(s) 1023
EcoRV GATATC 1 cut(s) 1023
EcoT14I CCWWGG 1 cut(s) 438
EcoT22I ATGCAT 1 cut(s) 1081
EcoT38I GRGCYC 1 cut(s) 437
ErhI CCWWGG 1 cut(s) 438
FaeI CATG 4 cut(s) 161, 316, 442, 1146
FatI CATG 4 cut(s) 157, 312, 438, 1142
FokI GGATG 3 cut(s) 27, 295, 380
FriOI GRGCYC 1 cut(s) 437
GsuI CTGGAG 1 cut(s) 825
HaeIII GGCC 3 cut(s) 506, 645, 912
Hin1II CATG 4 cut(s) 161, 316, 442, 1146
HinfI GANTC 3 cut(s) 319, 1065, 1154
HphI GGTGA 1 cut(s) 226
Hpy166II GTNNAC 2 cut(s) 772, 873
Hpy188I TCNGA 5 cut(s) 103, 453, 628, 998, 1163
Hpy188III TCNNGA 3 cut(s) 521, 653, 1046
Hpy8I GTNNAC 2 cut(s) 772, 873
HpyAV CCTTC 2 cut(s) 272, 782
HpyCH4III ACNGT 9 cut(s) 56, 180, 270, 356, 377, 482, 556, 778, 1123
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 9 cut(s) 63, 86, 168, 611, 634, 821, 903, 1073, 1079
HpyF10VI GCNNNNNNNGC 2 cut(s) 900, 909
HpyF3I CTNAG 4 cut(s) 100, 627, 728, 938
HpySE526I ACGT 1 cut(s) 132
Hsp92II CATG 4 cut(s) 161, 316, 442, 1146
Kzo9I GATC 4 cut(s) 415, 495, 826, 1126
LmnI GCTCC 3 cut(s) 130, 440, 538
LpnPI CCDG 8 cut(s) 36, 351, 506, 579, 666, 789, 876, 1143
LweI GCATC 7 cut(s) 5, 73, 598, 621, 673, 1066, 1088
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 136, 371, 556
MalI GATC 4 cut(s) 417, 497, 828, 1128
MboI GATC 4 cut(s) 415, 495, 826, 1126
MboII GAAGA 5 cut(s) 17, 88, 232, 752, 823
MflI RGATCY 2 cut(s) 826, 1126
MhlI GDGCHC 1 cut(s) 437
MlsI TGGCCA 1 cut(s) 645
MluCI AATT 5 cut(s) 153, 169, 722, 931, 990
MluNI TGGCCA 1 cut(s) 645
MmeI TCCRAC 1 cut(s) 732
MnlI CCTC 8 cut(s) 5, 104, 157, 377, 496, 902, 992, 1053
Mox20I TGGCCA 1 cut(s) 645
Mph1103I ATGCAT 1 cut(s) 1081
MroXI GAANNNNTTC 2 cut(s) 95, 1041
MscI TGGCCA 1 cut(s) 645
MseI TTAA 4 cut(s) 444, 564, 917, 1149
Msp20I TGGCCA 1 cut(s) 645
MwoI GCNNNNNNNGC 2 cut(s) 900, 909
NcoI CCATGG 1 cut(s) 438
NdeII GATC 4 cut(s) 415, 495, 826, 1126
NlaIII CATG 4 cut(s) 161, 316, 442, 1146
NlaIV GGNNCC 1 cut(s) 436
NsiI ATGCAT 1 cut(s) 1081
PceI AGGCCT 1 cut(s) 912
PdmI GAANNNNTTC 2 cut(s) 95, 1041
PfeI GAWTC 3 cut(s) 319, 1065, 1154
Psp1406I AACGTT 1 cut(s) 132
PspN4I GGNNCC 1 cut(s) 436
PstI CTGCAG 1 cut(s) 1075
PsuI RGATCY 2 cut(s) 826, 1126
RsaI GTAC 1 cut(s) 979
RsaNI GTAC 1 cut(s) 978
SaqAI TTAA 4 cut(s) 444, 564, 917, 1149
Sau3AI GATC 4 cut(s) 415, 495, 826, 1126
SduI GDGCHC 1 cut(s) 437
SfaNI GCATC 7 cut(s) 5, 73, 598, 621, 673, 1066, 1088
SfcI CTRYAG 3 cut(s) 37, 835, 1071
Sse9I AATT 5 cut(s) 153, 169, 722, 931, 990
SseBI AGGCCT 1 cut(s) 912
SsiI CCGC 1 cut(s) 894
SspI AATATT 1 cut(s) 763
StuI AGGCCT 1 cut(s) 912
StyI CCWWGG 1 cut(s) 438
TaaI ACNGT 9 cut(s) 56, 180, 270, 356, 377, 482, 556, 778, 1123
TaiI ACGT 1 cut(s) 135
TaqI TCGA 1 cut(s) 498
TasI AATT 5 cut(s) 153, 169, 722, 931, 990
TfiI GAWTC 3 cut(s) 319, 1065, 1154
Tru1I TTAA 4 cut(s) 444, 564, 917, 1149
Tru9I TTAA 4 cut(s) 444, 564, 917, 1149
TscAI CASTG 2 cut(s) 185, 273
TspDTI ATGAA 4 cut(s) 104, 146, 222, 1022
TspGWI ACGGA 1 cut(s) 489
TspRI CASTG 2 cut(s) 185, 273
XapI RAATTY 1 cut(s) 931
XmnI GAANNNNTTC 2 cut(s) 95, 1041
Zsp2I ATGCAT 1 cut(s) 1081
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.