MD03G1012300.v1.1

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000144
Physical Location & Seq
Reverse (-)
936705 .. 940769
4065 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1012300.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGCTGGCGCCGAAGGAGACGAACGAGGACTTGGCTCTCCCCGACGATGCGTGCCCCGTTCTCAACGTCGGCGAGGAGAAGGAGATCGGCATGCAGGGCTTGGAGAAGAAGAATAACAGCGTCAACGAGGTTGAAGATGGGCGTTGGCAGAGACCACCTTTGGATATCATGGAGAATATCGTACAACGCCTCGACTTACTTGATCGGATTCGCTTAAGCATCCTCTGCAAGTCCTGGAGAATGATTGTTATGCAAATTATCCGCAGTGCTCCGCCATTCCCATGGTTAGTAGGCCCATCTGAAGCAGCTTCCTGGCTATTATTTGGAACTCAACCGAGTTCCAGTTACTTAGAGTTCTCCATCCCACCTGAAGGTAGAGTCATCAAGCTAGGGTTGCCTAAGCGAAATCAAGGGTGCTTTCATAGTTCCTCCAAAGGTTGGTTGATAATGGTCAATGAAGAACACTCTGAAGTATTTCTACTAAACCCTATTTCAGGAGCCAAACACGAACTTCCTTCCTTGAGAAGAATCCCATATTTCAAGAAATTTGGTGGAAAAGCCGACCGATTTGTTGACAAAATTGCACTATCGAGTTCAAATCTATCAGAATGTACGGTGGCAGCAATTTTTTCTTCTGGGGAAATTGGTGTGTGCAGGCCAAAAAAAGGTTGGAAAATATCTCGGATGTGGGATAGAAAAACGTGTCCTTCATGCCTACTGTTTTCGTCTTGTGGCATGCTATATGTCTTAGTCGAAAACACTGTCAAAAACAGTAAGTCTGGACACGTTGTAACTCGCACATTATGCCTCGGAGATGGTGATGAGCTGGAATTGAAGTTGGTTTATGACAAAGAAAAATCACGAAAGGGTTCATTCAACTGCTGGGGTGAAGGTGACTTTTTGGATGTCAAGGTCAAAGACGAGTCATATTTGATAGAATCAACAACCAGCAACGAAGTCTTGTTGATCCGTCAAATAAAGGATCAAATGAAAACCGATTTGAATACCTGGACAAGGACTCATGATTTTCTAGTTTACAAGATCGATCCGGAGAATGGTGAAAGCTTTATCGAGGTACAGAGTTTGGGGAATCAAATATTATTTGTAACAAGCCGAGGCTGTTCCTTTTCCTTTCCAGTGAGTAATTCCAACGGGTCGGAAGCAAACTGCATTTATTTTGTGGAGTTAGGAGGAATTGACCACGACGACATGTTTTGTGTTGAGAATTCTGGACATAGTAGAACCACCATGTGTGATTTTTCTCATCCTTTCTACTTAGATGGCCGGAAATTTGGAAGATCCAAAAAATTTTATGATAGTATTGAAACTGAATTCACTTGGGGAGTGAGTTGGTACAGTCCGAGTTTACAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

458

Amino Acids

51.72

Weight (kDa)

5.88

Isoelectric Point (pI)

44.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 53 - 83 1.5e-07 F-box domain
Beta-prop_KIB1-4 PF03478 123 - 404 2.1e-34 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 7
AccB7I CCANNNNNTGG 1 cut(s) 438
AccIII TCCGGA 1 cut(s) 1048
AciI CCGC 2 cut(s) 262, 272
AclWI GGATC 4 cut(s) 961, 990, 1040, 1293
AcoI YGGCCR 1 cut(s) 1282
AcsI RAATTY 5 cut(s) 545, 1225, 1289, 1307, 1331
AcuI CTGAAG 3 cut(s) 321, 390, 489
AcyI GRCGYC 1 cut(s) 8
AdeI CACNNNGTG 1 cut(s) 1251
AfaI GTAC 4 cut(s) 183, 613, 1077, 1355
AfiI CCNNNNNNNGG 6 cut(s) 371, 438, 494, 665, 1014, 1055
AflII CTTAAG 1 cut(s) 214
AflIII ACRYGT 3 cut(s) 701, 784, 1209
AgsI TTSAA 7 cut(s) 134, 541, 597, 835, 877, 1003, 1325
AjnI CCWGG 3 cut(s) 233, 311, 1007
AjuI GAANNNNNNNTTGG 2 cut(s) 14, 46
AluBI AGCT 4 cut(s) 308, 388, 826, 1065
AluI AGCT 4 cut(s) 308, 388, 826, 1065
Alw21I GWGCWC 1 cut(s) 271
Alw26I GTCTC 2 cut(s) 11, 145
AlwI GGATC 4 cut(s) 961, 990, 1040, 1293
Aor13HI TCCGGA 1 cut(s) 1048
AoxI GGCC 3 cut(s) 292, 656, 1282
ApeKI GCWGC 2 cut(s) 305, 620
ApoI RAATTY 5 cut(s) 545, 1225, 1289, 1307, 1331
ArsI GACNNNNNNTTYG 2 cut(s) 747, 779
Asp700I GAANNNNTTC 2 cut(s) 474, 868
AspLEI GCGC 1 cut(s) 10
AspS9I GGNCC 1 cut(s) 293
AsuHPI GGTGA 4 cut(s) 830, 899, 905, 1070
BaeGI GKGCMC 1 cut(s) 56
BanI GGYRCC 1 cut(s) 7
BarI GAAGNNNNNNTAC 2 cut(s) 462, 494
Bbv12I GWGCWC 1 cut(s) 271
BbvI GCAGC 2 cut(s) 317, 632
BccI CCATC 5 cut(s) 131, 304, 368, 809, 1274
BciT130I CCWGG 3 cut(s) 235, 313, 1009
BcoDI GTCTC 2 cut(s) 11, 145
BfaI CTAG 2 cut(s) 389, 1031
BfoI RGCGCY 1 cut(s) 11
BfrI CTTAAG 1 cut(s) 214
BisI GCNGC 2 cut(s) 306, 621
BlsI GCNGC 2 cut(s) 307, 622
Bme1390I CCNGG 3 cut(s) 235, 313, 1009
BmgT120I GGNCC 1 cut(s) 293
BmiI GGNNCC 2 cut(s) 9, 499
BmrFI CCNGG 3 cut(s) 235, 313, 1009
BmsI GCATC 2 cut(s) 37, 228
BpmI CTGGAG 1 cut(s) 256
Bpu10I CCTNAGC 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 541
Bsa29I ATCGAT 1 cut(s) 1044
BsaHI GRCGYC 1 cut(s) 8
BsaI GGTCTC 1 cut(s) 145
BsaJI CCNNGG 3 cut(s) 281, 808, 1114
BsaWI WCCGGW 1 cut(s) 1048
Bsc4I CCNNNNNNNGG 6 cut(s) 371, 438, 494, 665, 1014, 1055
Bse1I ACTGG 2 cut(s) 342, 1136
BseAI TCCGGA 1 cut(s) 1048
BseBI CCWGG 3 cut(s) 235, 313, 1009
BseCI ATCGAT 1 cut(s) 1044
BseDI CCNNGG 3 cut(s) 281, 808, 1114
BseGI GGATG 5 cut(s) 219, 360, 690, 910, 1264
BseLI CCNNNNNNNGG 6 cut(s) 371, 438, 494, 665, 1014, 1055
BseNI ACTGG 2 cut(s) 342, 1136
BseRI GAGGAG 1 cut(s) 89
BseSI GKGCMC 1 cut(s) 56
BseXI GCAGC 2 cut(s) 317, 632
BseYI CCCAGC 1 cut(s) 882
BsgI GTGCAG 1 cut(s) 673
Bsh1285I CGRYCG 1 cut(s) 565
BshFI GGCC 3 cut(s) 294, 658, 1284
BshNI GGYRCC 1 cut(s) 7
BshVI ATCGAT 1 cut(s) 1044
BsiEI CGRYCG 1 cut(s) 565
BsiHKAI GWGCWC 1 cut(s) 271
BsiSI CCGG 2 cut(s) 1049, 1285
BslI CCNNNNNNNGG 6 cut(s) 371, 438, 494, 665, 1014, 1055
BsmAI GTCTC 2 cut(s) 11, 145
BsmBI CGTCTC 1 cut(s) 11
BsnI GGCC 3 cut(s) 294, 658, 1284
Bso31I GGTCTC 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 56, 271
Bsp13I TCCGGA 1 cut(s) 1048
Bsp143I GATC 7 cut(s) 84, 202, 966, 982, 1041, 1045, 1298
Bsp19I CCATGG 1 cut(s) 281
BspACI CCGC 2 cut(s) 262, 272
BspANI GGCC 3 cut(s) 294, 658, 1284
BspDI ATCGAT 1 cut(s) 1044
BspEI TCCGGA 1 cut(s) 1048
BspHI TCATGA 1 cut(s) 1021
BspLI GGNNCC 2 cut(s) 9, 499
BspPI GGATC 4 cut(s) 961, 990, 1040, 1293
BspT107I GGYRCC 1 cut(s) 7
BspTI CTTAAG 1 cut(s) 214
BspTNI GGTCTC 1 cut(s) 145
BsrI ACTGG 2 cut(s) 342, 1136
BssECI CCNNGG 3 cut(s) 281, 808, 1114
BssMI GATC 7 cut(s) 84, 202, 966, 982, 1041, 1045, 1298
BssNI GRCGYC 1 cut(s) 8
BssT1I CCWWGG 1 cut(s) 281
Bst2UI CCWGG 3 cut(s) 235, 313, 1009
Bst4CI ACNGT 6 cut(s) 616, 720, 763, 773, 1358, 1371
BstACI GRCGYC 1 cut(s) 8
BstAFI CTTAAG 1 cut(s) 214
BstAPI GCANNNNNTGC 2 cut(s) 225, 804
BstC8I GCNNGC 5 cut(s) 6, 52, 92, 656, 737
BstDEI CTNAG 4 cut(s) 349, 399, 748, 1276
BstDSI CCRYGG 1 cut(s) 281
BstENI CCTNNNNNAGG 2 cut(s) 492, 1012
BstF5I GGATG 5 cut(s) 219, 360, 690, 910, 1264
BstH2I RGCGCY 1 cut(s) 11
BstHHI GCGC 1 cut(s) 10
BstKTI GATC 7 cut(s) 87, 205, 969, 985, 1044, 1048, 1301
BstMAI GTCTC 2 cut(s) 11, 145
BstMBI GATC 7 cut(s) 84, 202, 966, 982, 1041, 1045, 1298
BstMCI CGRYCG 1 cut(s) 565
BstMWI GCNNNNNNNGC 4 cut(s) 96, 225, 394, 804
BstNI CCWGG 3 cut(s) 235, 313, 1009
BstNSI RCATGY 3 cut(s) 94, 739, 1213
BstSCI CCNGG 3 cut(s) 233, 311, 1007
BstSLI GKGCMC 1 cut(s) 56
BstV1I GCAGC 2 cut(s) 317, 632
BstX2I RGATCY 1 cut(s) 1298
BstXI CCANNNNNNTGG 1 cut(s) 282
BstYI RGATCY 1 cut(s) 1298
Bsu15I ATCGAT 1 cut(s) 1044
BsuRI GGCC 3 cut(s) 294, 658, 1284
BsuTUI ATCGAT 1 cut(s) 1044
BtgI CCRYGG 1 cut(s) 281
BtsCI GGATG 5 cut(s) 219, 360, 690, 910, 1264
BtsI GCAGTG 1 cut(s) 271
BtsIMutI CAGTG 3 cut(s) 271, 759, 1143
Cac8I GCNNGC 5 cut(s) 6, 52, 92, 656, 737
CciI TCATGA 1 cut(s) 1021
CfoI GCGC 1 cut(s) 10
Cfr13I GGNCC 1 cut(s) 293
ClaI ATCGAT 1 cut(s) 1044
CseI GACGC 1 cut(s) 109
Csp6I GTAC 4 cut(s) 182, 612, 1076, 1354
CviAII CATG 8 cut(s) 91, 169, 282, 711, 736, 1022, 1210, 1249
CviQI GTAC 4 cut(s) 182, 612, 1076, 1354
DdeI CTNAG 4 cut(s) 349, 399, 748, 1276
DinI GGCGCC 1 cut(s) 9
DpnI GATC 7 cut(s) 86, 204, 968, 984, 1043, 1047, 1300
DpnII GATC 7 cut(s) 84, 202, 966, 982, 1041, 1045, 1298
DraIII CACNNNGTG 1 cut(s) 1251
EaeI YGGCCR 1 cut(s) 1282
EciI GGCGGA 1 cut(s) 261
Eco130I CCWWGG 1 cut(s) 281
Eco31I GGTCTC 1 cut(s) 145
Eco32I GATATC 1 cut(s) 166
Eco57I CTGAAG 3 cut(s) 321, 390, 489
EcoNI CCTNNNNNAGG 2 cut(s) 492, 1012
EcoRI GAATTC 2 cut(s) 1225, 1331
EcoRII CCWGG 3 cut(s) 233, 311, 1007
EcoRV GATATC 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 281
EgeI GGCGCC 1 cut(s) 9
EheI GGCGCC 1 cut(s) 9
ErhI CCWWGG 1 cut(s) 281
Esp3I CGTCTC 1 cut(s) 11
FaeI CATG 8 cut(s) 94, 172, 285, 714, 739, 1025, 1213, 1252
FatI CATG 8 cut(s) 90, 168, 281, 710, 735, 1021, 1209, 1248
Fnu4HI GCNGC 2 cut(s) 306, 621
FokI GGATG 5 cut(s) 206, 347, 697, 917, 1251
Fsp4HI GCNGC 2 cut(s) 306, 621
FspBI CTAG 2 cut(s) 389, 1031
GlaI GCGC 1 cut(s) 9
GluI GCNGC 2 cut(s) 306, 621
GsaI CCCAGC 1 cut(s) 886
GsuI CTGGAG 1 cut(s) 256
HaeII RGCGCY 1 cut(s) 11
HaeIII GGCC 3 cut(s) 294, 658, 1284
HapII CCGG 2 cut(s) 1049, 1285
HgaI GACGC 1 cut(s) 109
HhaI GCGC 1 cut(s) 10
Hin1I GRCGYC 1 cut(s) 8
Hin1II CATG 8 cut(s) 94, 172, 285, 714, 739, 1025, 1213, 1252
Hin6I GCGC 1 cut(s) 8
HinP1I GCGC 1 cut(s) 8
HincII GTYRAC 2 cut(s) 124, 574
HindII GTYRAC 2 cut(s) 124, 574
HindIII AAGCTT 1 cut(s) 1063
HinfI GANTC 7 cut(s) 208, 378, 528, 923, 938, 1018, 1090
HpaII CCGG 2 cut(s) 1049, 1285
HphI GGTGA 4 cut(s) 830, 899, 905, 1070
Hpy166II GTNNAC 4 cut(s) 124, 574, 1036, 1367
Hpy188I TCNGA 8 cut(s) 207, 301, 469, 607, 684, 812, 1159, 1362
Hpy188III TCNNGA 7 cut(s) 495, 541, 780, 861, 1022, 1049, 1230
Hpy8I GTNNAC 4 cut(s) 124, 574, 1036, 1367
Hpy99I CGWCG 3 cut(s) 47, 71, 1208
HpyAV CCTTC 6 cut(s) 7, 73, 365, 525, 717, 884
HpyCH4III ACNGT 6 cut(s) 616, 720, 763, 773, 1358, 1371
HpyCH4IV ACGT 3 cut(s) 66, 701, 786
HpyCH4V TGCA 6 cut(s) 94, 228, 253, 584, 654, 1170
HpyF10VI GCNNNNNNNGC 4 cut(s) 96, 225, 394, 804
HpyF3I CTNAG 4 cut(s) 349, 399, 748, 1276
HpySE526I ACGT 3 cut(s) 66, 701, 786
Hsp92I GRCGYC 1 cut(s) 8
Hsp92II CATG 8 cut(s) 94, 172, 285, 714, 739, 1025, 1213, 1252
HspAI GCGC 1 cut(s) 8
KasI GGCGCC 1 cut(s) 7
Kpn2I TCCGGA 1 cut(s) 1048
Kzo9I GATC 7 cut(s) 84, 202, 966, 982, 1041, 1045, 1298
LmnI GCTCC 2 cut(s) 274, 497
Lsp1109I GCAGC 2 cut(s) 317, 632
LweI GCATC 2 cut(s) 37, 228
MaeI CTAG 2 cut(s) 389, 1031
MaeII ACGT 3 cut(s) 66, 701, 786
MaeIII GTNAC 4 cut(s) 344, 790, 893, 1105
MalI GATC 7 cut(s) 86, 204, 968, 984, 1043, 1047, 1300
MboI GATC 7 cut(s) 84, 202, 966, 982, 1041, 1045, 1298
MboII GAAGA 7 cut(s) 118, 121, 146, 470, 537, 624, 1308
MflI RGATCY 1 cut(s) 1298
MhlI GDGCHC 2 cut(s) 56, 271
Mly113I GGCGCC 1 cut(s) 8
MlyI GAGTC 3 cut(s) 387, 932, 1012
MmeI TCCRAC 3 cut(s) 650, 1137, 1173
MroI TCCGGA 1 cut(s) 1048
MroXI GAANNNNTTC 2 cut(s) 474, 868
MseI TTAA 1 cut(s) 215
MslI CAYNNNNRTG 1 cut(s) 280
MspCI CTTAAG 1 cut(s) 214
MspI CCGG 2 cut(s) 1049, 1285
MspR9I CCNGG 3 cut(s) 235, 313, 1009
MvaI CCWGG 3 cut(s) 235, 313, 1009
MwoI GCNNNNNNNGC 4 cut(s) 96, 225, 394, 804
NarI GGCGCC 1 cut(s) 8
NcoI CCATGG 1 cut(s) 281
NdeII GATC 7 cut(s) 84, 202, 966, 982, 1041, 1045, 1298
NlaIII CATG 8 cut(s) 94, 172, 285, 714, 739, 1025, 1213, 1252
NlaIV GGNNCC 2 cut(s) 9, 499
NmeAIII GCCGAG 1 cut(s) 1139
NmuCI GTSAC 1 cut(s) 893
NspI RCATGY 3 cut(s) 94, 739, 1213
PaeI GCATGC 2 cut(s) 94, 739
PagI TCATGA 1 cut(s) 1021
PciI ACATGT 1 cut(s) 1209
PdmI GAANNNNTTC 2 cut(s) 474, 868
PfeI GAWTC 4 cut(s) 208, 528, 938, 1090
PflMI CCANNNNNTGG 1 cut(s) 438
PfoI TCCNGGA 1 cut(s) 233
PkrI GCNGC 2 cut(s) 307, 622
PleI GAGTC 3 cut(s) 386, 931, 1012
PluTI GGCGCC 1 cut(s) 11
PpsI GAGTC 3 cut(s) 386, 931, 1012
PscI ACATGT 1 cut(s) 1209
Psp6I CCWGG 3 cut(s) 233, 311, 1007
PspFI CCCAGC 1 cut(s) 882
PspGI CCWGG 3 cut(s) 233, 311, 1007
PspN4I GGNNCC 2 cut(s) 9, 499
PspPI GGNCC 1 cut(s) 293
PsuI RGATCY 1 cut(s) 1298
RsaI GTAC 4 cut(s) 183, 613, 1077, 1355
RsaNI GTAC 4 cut(s) 182, 612, 1076, 1354
RseI CAYNNNNRTG 1 cut(s) 280
SaqAI TTAA 1 cut(s) 215
SatI GCNGC 2 cut(s) 306, 621
Sau3AI GATC 7 cut(s) 84, 202, 966, 982, 1041, 1045, 1298
Sau96I GGNCC 1 cut(s) 293
SchI GAGTC 3 cut(s) 387, 932, 1012
ScrFI CCNGG 3 cut(s) 235, 313, 1009
SduI GDGCHC 2 cut(s) 56, 271
SfaNI GCATC 2 cut(s) 37, 228
SfoI GGCGCC 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 280
SmlI CTYRAG 2 cut(s) 214, 520
SmoI CTYRAG 2 cut(s) 214, 520
SphI GCATGC 2 cut(s) 94, 739
SsiI CCGC 2 cut(s) 262, 272
SspDI GGCGCC 1 cut(s) 7
SspI AATATT 1 cut(s) 1098
SspMI CTAG 2 cut(s) 389, 1031
StyD4I CCNGG 3 cut(s) 233, 311, 1007
StyI CCWWGG 1 cut(s) 281
TaaI ACNGT 6 cut(s) 616, 720, 763, 773, 1358, 1371
TaiI ACGT 3 cut(s) 69, 704, 789
TaqI TCGA 5 cut(s) 192, 590, 753, 1044, 1071
TaqII GACCGA 1 cut(s) 579
TfiI GAWTC 4 cut(s) 208, 528, 938, 1090
Tru1I TTAA 1 cut(s) 215
Tru9I TTAA 1 cut(s) 215
TscAI CASTG 3 cut(s) 271, 766, 1143
TseFI GTSAC 1 cut(s) 893
TseI GCWGC 2 cut(s) 305, 620
Tsp45I GTSAC 1 cut(s) 893
TspDTI ATGAA 5 cut(s) 410, 471, 699, 861, 1004
TspGWI ACGGA 1 cut(s) 959
TspRI CASTG 3 cut(s) 271, 766, 1143
Van91I CCANNNNNTGG 1 cut(s) 438
Vha464I CTTAAG 1 cut(s) 214
XagI CCTNNNNNAGG 2 cut(s) 492, 1012
XapI RAATTY 5 cut(s) 545, 1225, 1289, 1307, 1331
XceI RCATGY 3 cut(s) 94, 739, 1213
XcmI CCANNNNNNNNNTGG 1 cut(s) 666
XmnI GAANNNNTTC 2 cut(s) 474, 868
XspI CTAG 2 cut(s) 389, 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.