Rh6AG245300

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000144
Physical Location & Seq
Forward (+)
43401693 .. 43402364
672 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG245300.1

Sequence Viewer

Length: 672 bp
ATGCTCTTAACTGTGATTAAGACTCTATTTTTGGCTCAAAAGAATGTGGGATACTACCTCGCGCTAACCATATCTGGTTTCCTTAAGAGCCTTCAAACTTATTGTTATCAGAGACTAGACAACAAGAAAAAGCAAAATGACTTGGGTCATTGGCAGGAACCACCTGAAGATATCAAAGAGATGATTGTTCAGCACATGAGTTTCAGTGGTCGAGTTCGTTTAAGCAGTTTGTGCAAGTCTTGGAGGTTGGTTTCTATGCGAAGAGATATCCGTTCTCCTAGACAACTCCCATGGTTAGTACACCCTCAACTACCAAACTGTCATACCTACTTGAGATTCTCAAGCCTCTCTGAGGGCAAAGTTTTCAAGCTGAGGCTGCCTAAGCCGTTTTTCATGAAGTCGAGGCTGGCCAGGCTAGTTCCGAGATGTTTCCACCTGCAATGGTTTATTTGTTCTTCCAAAGGTTGGTTGATTTTGTGGAAGTGGGACTATGTCGGGCTAAAACGTGAGACGTTTATCTTTCTATTAAATCCAGTTTCAGGAGCCCAACACCAACTTCCATCGTTAAAAACAATTCCATCTTATGGAAAACATTCTACCATTGATGGATTGGTTTTATGCAGCCCAGACATCTCAGAATGTATGGTGGCAGCAGTTCAACAGAATATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

223

Amino Acids

26.01

Weight (kDa)

9.9

Isoelectric Point (pI)

50.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 114 - 221 6.6e-09 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 444
Acc36I ACCTGC 1 cut(s) 444
AccB7I CCANNNNNTGG 2 cut(s) 465, 584
AccII CGCG 1 cut(s) 62
AcoI YGGCCR 1 cut(s) 408
AcuI CTGAAG 1 cut(s) 186
AfaI GTAC 1 cut(s) 300
AfiI CCNNNNNNNGG 4 cut(s) 352, 465, 539, 584
AflII CTTAAG 1 cut(s) 83
AgsI TTSAA 3 cut(s) 95, 367, 659
AjnI CCWGG 1 cut(s) 410
AjuI GAANNNNNNNTTGG 2 cut(s) 540, 572
AluBI AGCT 1 cut(s) 370
AluI AGCT 1 cut(s) 370
Alw26I GTCTC 2 cut(s) 106, 503
AoxI GGCC 1 cut(s) 408
ApeKI GCWGC 3 cut(s) 376, 621, 650
ArsI GACNNNNNNTTYG 2 cut(s) 13, 45
Asp700I GAANNNNTTC 1 cut(s) 592
AspLEI GCGC 1 cut(s) 64
BalI TGGCCA 1 cut(s) 410
BanII GRGCYC 1 cut(s) 547
BbvCI CCTCAGC 1 cut(s) 371
BbvI GCAGC 3 cut(s) 363, 633, 662
BccI CCATC 3 cut(s) 568, 586, 599
BceAI ACGGC 1 cut(s) 370
BciT130I CCWGG 1 cut(s) 412
BciVI GTATCC 1 cut(s) 44
BcoDI GTCTC 2 cut(s) 106, 503
BfaI CTAG 3 cut(s) 116, 279, 416
BfrI CTTAAG 1 cut(s) 83
BfuAI ACCTGC 1 cut(s) 444
BfuI GTATCC 1 cut(s) 44
BisI GCNGC 3 cut(s) 377, 622, 651
BlsI GCNGC 3 cut(s) 378, 623, 652
Bme1390I CCNGG 1 cut(s) 412
BmiI GGNNCC 2 cut(s) 159, 544
BmrFI CCNGG 1 cut(s) 412
BoxI GACNNNNGTC 1 cut(s) 144
Bpu10I CCTNAGC 2 cut(s) 371, 381
BpuEI CTTGAG 2 cut(s) 325, 352
BsaJI CCNNGG 1 cut(s) 290
Bsc4I CCNNNNNNNGG 4 cut(s) 352, 465, 539, 584
Bse1I ACTGG 1 cut(s) 533
Bse3DI GCAATG 1 cut(s) 446
BseBI CCWGG 1 cut(s) 412
BseDI CCNNGG 1 cut(s) 290
BseLI CCNNNNNNNGG 4 cut(s) 352, 465, 539, 584
BseMI GCAATG 1 cut(s) 446
BseMII CTCAG 3 cut(s) 342, 362, 648
BseNI ACTGG 1 cut(s) 533
BseXI GCAGC 3 cut(s) 363, 633, 662
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 1 cut(s) 410
BslFI GGGAC 1 cut(s) 500
BslI CCNNNNNNNGG 4 cut(s) 352, 465, 539, 584
BsmAI GTCTC 2 cut(s) 106, 503
BsmBI CGTCTC 1 cut(s) 503
BsmFI GGGAC 1 cut(s) 500
BsnI GGCC 1 cut(s) 410
Bsp1286I GDGCHC 1 cut(s) 547
Bsp19I CCATGG 1 cut(s) 290
BspANI GGCC 1 cut(s) 410
BspCNI CTCAG 3 cut(s) 343, 363, 647
BspFNI CGCG 1 cut(s) 62
BspHI TCATGA 1 cut(s) 393
BspLI GGNNCC 2 cut(s) 159, 544
BspMI ACCTGC 1 cut(s) 444
BspTI CTTAAG 1 cut(s) 83
BsrDI GCAATG 1 cut(s) 446
BsrI ACTGG 1 cut(s) 533
BssECI CCNNGG 1 cut(s) 290
BssT1I CCWWGG 1 cut(s) 290
Bst2UI CCWGG 1 cut(s) 412
Bst4CI ACNGT 2 cut(s) 13, 320
Bst6I CTCTTC 1 cut(s) 256
BstAFI CTTAAG 1 cut(s) 83
BstAPI GCANNNNNTGC 1 cut(s) 231
BstC8I GCNNGC 1 cut(s) 408
BstDEI CTNAG 4 cut(s) 351, 371, 381, 634
BstDSI CCRYGG 1 cut(s) 290
BstENI CCTNNNNNAGG 1 cut(s) 350
BstFNI CGCG 1 cut(s) 62
BstHHI GCGC 1 cut(s) 64
BstMAI GTCTC 2 cut(s) 106, 503
BstMWI GCNNNNNNNGC 4 cut(s) 231, 376, 382, 412
BstNI CCWGG 1 cut(s) 412
BstPAI GACNNNNGTC 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 410
BstUI CGCG 1 cut(s) 62
BstV1I GCAGC 3 cut(s) 363, 633, 662
BsuI GTATCC 1 cut(s) 44
BsuRI GGCC 1 cut(s) 410
BtgI CCRYGG 1 cut(s) 290
BtsIMutI CAGTG 1 cut(s) 211
BveI ACCTGC 1 cut(s) 444
Cac8I GCNNGC 1 cut(s) 408
CciI TCATGA 1 cut(s) 393
CfoI GCGC 1 cut(s) 64
Csp6I GTAC 1 cut(s) 299
CviAII CATG 3 cut(s) 196, 291, 394
CviQI GTAC 1 cut(s) 299
DdeI CTNAG 4 cut(s) 351, 371, 381, 634
EaeI YGGCCR 1 cut(s) 408
Eam1104I CTCTTC 1 cut(s) 256
EarI CTCTTC 1 cut(s) 256
Eco130I CCWWGG 1 cut(s) 290
Eco24I GRGCYC 1 cut(s) 547
Eco32I GATATC 2 cut(s) 172, 268
Eco57I CTGAAG 1 cut(s) 186
EcoNI CCTNNNNNAGG 1 cut(s) 350
EcoRII CCWGG 1 cut(s) 410
EcoRV GATATC 2 cut(s) 172, 268
EcoT14I CCWWGG 1 cut(s) 290
EcoT38I GRGCYC 1 cut(s) 547
ErhI CCWWGG 1 cut(s) 290
Esp3I CGTCTC 1 cut(s) 503
FaeI CATG 3 cut(s) 199, 294, 397
FaqI GGGAC 1 cut(s) 500
FatI CATG 3 cut(s) 195, 290, 393
Fnu4HI GCNGC 3 cut(s) 377, 622, 651
FriOI GRGCYC 1 cut(s) 547
Fsp4HI GCNGC 3 cut(s) 377, 622, 651
FspBI CTAG 3 cut(s) 116, 279, 416
GlaI GCGC 1 cut(s) 63
GluI GCNGC 3 cut(s) 377, 622, 651
HaeIII GGCC 1 cut(s) 410
HhaI GCGC 1 cut(s) 64
Hin1II CATG 3 cut(s) 199, 294, 397
Hin6I GCGC 1 cut(s) 62
HinP1I GCGC 1 cut(s) 62
HinfI GANTC 2 cut(s) 22, 336
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 4 cut(s) 111, 352, 423, 637
Hpy188III TCNNGA 2 cut(s) 394, 540
Hpy8I GTNNAC 1 cut(s) 301
HpyAV CCTTC 1 cut(s) 101
HpyCH4III ACNGT 2 cut(s) 13, 320
HpyCH4IV ACGT 2 cut(s) 505, 512
HpyCH4V TGCA 3 cut(s) 234, 439, 621
HpyF10VI GCNNNNNNNGC 4 cut(s) 231, 376, 382, 412
HpyF3I CTNAG 4 cut(s) 351, 371, 381, 634
HpySE526I ACGT 2 cut(s) 505, 512
Hsp92II CATG 3 cut(s) 199, 294, 397
HspAI GCGC 1 cut(s) 62
LmnI GCTCC 1 cut(s) 542
Lsp1109I GCAGC 3 cut(s) 363, 633, 662
MaeI CTAG 3 cut(s) 116, 279, 416
MaeII ACGT 2 cut(s) 505, 512
MboII GAAGA 3 cut(s) 179, 273, 447
MhlI GDGCHC 1 cut(s) 547
MlsI TGGCCA 1 cut(s) 410
MluCI AATT 1 cut(s) 573
MluNI TGGCCA 1 cut(s) 410
MlyI GAGTC 1 cut(s) 16
MnlI CCTC 7 cut(s) 68, 237, 315, 346, 356, 366, 396
Mox20I TGGCCA 1 cut(s) 410
MroXI GAANNNNTTC 1 cut(s) 592
MscI TGGCCA 1 cut(s) 410
MseI TTAA 6 cut(s) 8, 18, 84, 221, 527, 566
Msp20I TGGCCA 1 cut(s) 410
MspCI CTTAAG 1 cut(s) 83
MspR9I CCNGG 1 cut(s) 412
MvaI CCWGG 1 cut(s) 412
MvnI CGCG 1 cut(s) 62
MwoI GCNNNNNNNGC 4 cut(s) 231, 376, 382, 412
NcoI CCATGG 1 cut(s) 290
NlaIII CATG 3 cut(s) 199, 294, 397
NlaIV GGNNCC 2 cut(s) 159, 544
PagI TCATGA 1 cut(s) 393
PaqCI CACCTGC 1 cut(s) 444
PdmI GAANNNNTTC 1 cut(s) 592
PfeI GAWTC 1 cut(s) 336
PflFI GACNNNGTC 1 cut(s) 491
PflMI CCANNNNNTGG 2 cut(s) 465, 584
PkrI GCNGC 3 cut(s) 378, 623, 652
PleI GAGTC 1 cut(s) 16
PpsI GAGTC 1 cut(s) 16
PshAI GACNNNNGTC 1 cut(s) 144
Psp6I CCWGG 1 cut(s) 410
PspGI CCWGG 1 cut(s) 410
PspN4I GGNNCC 2 cut(s) 159, 544
PsyI GACNNNGTC 1 cut(s) 491
RsaI GTAC 1 cut(s) 300
RsaNI GTAC 1 cut(s) 299
SaqAI TTAA 6 cut(s) 8, 18, 84, 221, 527, 566
SatI GCNGC 3 cut(s) 377, 622, 651
SchI GAGTC 1 cut(s) 16
ScrFI CCNGG 1 cut(s) 412
SduI GDGCHC 1 cut(s) 547
SetI ASST 9 cut(s) 60, 166, 248, 329, 372, 438, 466, 508, 515
SmlI CTYRAG 3 cut(s) 83, 331, 340
SmoI CTYRAG 3 cut(s) 83, 331, 340
Sse9I AATT 1 cut(s) 573
SspI AATATT 1 cut(s) 667
SspMI CTAG 3 cut(s) 116, 279, 416
StyD4I CCNGG 1 cut(s) 410
StyI CCWWGG 1 cut(s) 290
TaaI ACNGT 2 cut(s) 13, 320
TaiI ACGT 2 cut(s) 508, 515
TaqI TCGA 2 cut(s) 211, 401
TasI AATT 1 cut(s) 573
TatI WGTACW 1 cut(s) 298
TfiI GAWTC 1 cut(s) 336
Tru1I TTAA 6 cut(s) 8, 18, 84, 221, 527, 566
Tru9I TTAA 6 cut(s) 8, 18, 84, 221, 527, 566
TscAI CASTG 1 cut(s) 211
TseI GCWGC 3 cut(s) 376, 621, 650
TspDTI ATGAA 2 cut(s) 382, 410
TspGWI ACGGA 1 cut(s) 260
TspRI CASTG 1 cut(s) 211
Tth111I GACNNNGTC 1 cut(s) 491
Van91I CCANNNNNTGG 2 cut(s) 465, 584
Vha464I CTTAAG 1 cut(s) 83
XagI CCTNNNNNAGG 1 cut(s) 350
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmnI GAANNNNTTC 1 cut(s) 592
XspI CTAG 3 cut(s) 116, 279, 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.