Rroxscaffold_5G00338370

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000144
Physical Location & Seq
Forward (+)
6518486 .. 6520476
1991 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00338370.1

Sequence Viewer

Length: 933 bp
ATGTCGTCGTTTCGTCAACCTAAACCACAGCGAGCGAAGATGAAAGCCGAAGGTCAAATCCAGATGCAGCTCGTCGAAGGCGAAGACATCGACGATGAAGACGACCTCTTCGAAGCCATTGATAAGCATTCTGGTTTACCTCTTACAGCAAAACAAGGACTTGAGAATTCCCAATTAAACCTTAACTTGGAGTCAAACAGCAAGGATCTGTTAGATTCTTTTCCTAATCGCAAAAGCCAGATAGAGGTGATGATTGATACTCTGGTCATTTGCGGTTGCTTAGTGTTTGGTGGTAGTGTGATCAAAAGACTACTCTGTAGCAGACCCAGACCCTGGGAGACATTACCTGACGATGTCATGGAGATGGTTGTGCCGCACCTGAGTGTACGTGATCGTATACGATTAAGCAGTGTTTGCAAGTCTTGGAACACCGTTCCTATGCGCAGAGATATCCCTAGTGCTCCACATCAACTCCCCTGGTTAATACTTCCTCAATGTTGCCATAAGAGCTTGAGCTTTTACGACCTCTATGACGGCAAAGTTGGCAAGTTGGACCTGCCTGAACCAGTTCAAGGAGGGTGGTTTCAAGGATCTTCTAAAGGTTGGATGATCATGGTCATGGAAAAAGATCTAGATTCTACAATGTTTCTAGTAAATCCCATTTCAGGAGCCCAACACCAACTTCCATCTTTGAGCACGGTTCCATCTTTCCAAAATTTTGTAGAAACGACCGGATGGAAACGCTATGGTGCCTCCCGTTTCAGCTATTGCCTTGTGTTGTCCACCTCGGATATCACTTCAGAGCAATGTATGATAGCAGTAAATCAGTTTCCATTGAGGAGTATTAGATCTGTCTCAAAGGGATCCGCAAAGGATTTTCTTCCTCACTCGGATCCATCTCAAGGGGATATTACCTCTCTAAGATCTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

310

Amino Acids

34.79

Weight (kDa)

6.09

Isoelectric Point (pI)

51.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 112 - 144 5.2e-07 F-box domain
F-box-like PF12937 112 - 144 5e-07 F-box-like
Beta-prop_KIB1-4 PF03478 173 - 278 3.8e-12 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 443
Acc36I ACCTGC 1 cut(s) 564
AccB1I GGYRCC 1 cut(s) 749
AccB7I CCANNNNNTGG 1 cut(s) 333
AccI GTMKAC 1 cut(s) 397
AciI CCGC 3 cut(s) 273, 374, 867
AclWI GGATC 6 cut(s) 213, 598, 858, 871, 887, 900
AcsI RAATTY 2 cut(s) 166, 715
AcuI CTGAAG 1 cut(s) 783
AfaI GTAC 1 cut(s) 387
AfiI CCNNNNNNNGG 6 cut(s) 187, 244, 333, 572, 665, 902
AgsI TTSAA 2 cut(s) 572, 587
AjnI CCWGG 2 cut(s) 332, 476
AjuI GAANNNNNNNTTGG 2 cut(s) 666, 698
AleI CACNNNNGTG 1 cut(s) 381
AluBI AGCT 4 cut(s) 70, 510, 516, 765
AluI AGCT 4 cut(s) 70, 510, 516, 765
Alw21I GWGCWC 2 cut(s) 463, 698
Alw26I GTCTC 2 cut(s) 332, 859
AlwI GGATC 6 cut(s) 213, 598, 858, 871, 887, 900
AlwNI CAGNNNCTG 1 cut(s) 333
ApeKI GCWGC 1 cut(s) 67
ApoI RAATTY 2 cut(s) 166, 715
ArsI GACNNNNNNTTYG 2 cut(s) 92, 124
Asp700I GAANNNNTTC 1 cut(s) 567
AspLEI GCGC 1 cut(s) 444
AspS9I GGNCC 1 cut(s) 553
AsuHPI GGTGA 1 cut(s) 259
AsuII TTCGAA 1 cut(s) 111
AvaII GGWCC 1 cut(s) 553
BaeI ACNNNNGTAYC 2 cut(s) 249, 282
BamHI GGATCC 2 cut(s) 863, 892
BanI GGYRCC 1 cut(s) 749
BanII GRGCYC 1 cut(s) 673
BbsI GAAGAC 2 cut(s) 90, 105
Bbv12I GWGCWC 2 cut(s) 463, 698
BbvI GCAGC 1 cut(s) 79
BccI CCATC 5 cut(s) 358, 694, 712, 729, 904
BceAI ACGGC 1 cut(s) 550
BciT130I CCWGG 2 cut(s) 334, 478
BclI TGATCA 2 cut(s) 300, 609
BcoDI GTCTC 2 cut(s) 332, 859
BfaI CTAG 4 cut(s) 456, 632, 650, 931
BfmI CTRYAG 1 cut(s) 316
BfuAI ACCTGC 1 cut(s) 564
BglII AGATCT 3 cut(s) 628, 848, 923
BisI GCNGC 2 cut(s) 68, 374
BlsI GCNGC 2 cut(s) 69, 375
Bme1390I CCNGG 2 cut(s) 334, 478
Bme18I GGWCC 1 cut(s) 553
BmgT120I GGNCC 1 cut(s) 553
BmiI GGNNCC 5 cut(s) 670, 702, 751, 865, 894
BmrFI CCNGG 2 cut(s) 334, 478
BmsI GCATC 1 cut(s) 54
BpiI GAAGAC 2 cut(s) 90, 105
Bpu14I TTCGAA 1 cut(s) 111
BpuEI CTTGAG 3 cut(s) 182, 532, 885
BsaAI YACGTR 1 cut(s) 389
BsaJI CCNNGG 4 cut(s) 332, 333, 476, 786
BsaWI WCCGGW 1 cut(s) 731
BsaXI ACNNNNNCTCC 2 cut(s) 456, 486
Bsc4I CCNNNNNNNGG 6 cut(s) 187, 244, 333, 572, 665, 902
Bse1I ACTGG 1 cut(s) 566
Bse3DI GCAATG 1 cut(s) 812
BseBI CCWGG 2 cut(s) 334, 478
BseDI CCNNGG 4 cut(s) 332, 333, 476, 786
BseGI GGATG 2 cut(s) 612, 740
BseLI CCNNNNNNNGG 6 cut(s) 187, 244, 333, 572, 665, 902
BseMI GCAATG 1 cut(s) 812
BseMII CTCAG 1 cut(s) 371
BseNI ACTGG 1 cut(s) 566
BseRI GAGGAG 1 cut(s) 853
BseXI GCAGC 1 cut(s) 79
Bsh1285I CGRYCG 1 cut(s) 732
BshNI GGYRCC 1 cut(s) 749
BsiEI CGRYCG 1 cut(s) 732
BsiHKAI GWGCWC 2 cut(s) 463, 698
BsiSI CCGG 1 cut(s) 732
BslI CCNNNNNNNGG 6 cut(s) 187, 244, 333, 572, 665, 902
BsmAI GTCTC 2 cut(s) 332, 859
BsmI GAATGC 1 cut(s) 127
Bsp119I TTCGAA 1 cut(s) 111
Bsp1286I GDGCHC 3 cut(s) 463, 673, 698
BspACI CCGC 3 cut(s) 273, 374, 867
BspCNI CTCAG 1 cut(s) 372
BspLI GGNNCC 5 cut(s) 670, 702, 751, 865, 894
BspMI ACCTGC 1 cut(s) 564
BspPI GGATC 6 cut(s) 213, 598, 858, 871, 887, 900
BspT104I TTCGAA 1 cut(s) 111
BspT107I GGYRCC 1 cut(s) 749
BsrDI GCAATG 1 cut(s) 812
BsrI ACTGG 1 cut(s) 566
BssECI CCNNGG 4 cut(s) 332, 333, 476, 786
BssNAI GTATAC 1 cut(s) 398
Bst1107I GTATAC 1 cut(s) 398
Bst2UI CCWGG 2 cut(s) 334, 478
Bst4CI ACNGT 2 cut(s) 433, 700
Bst6I CTCTTC 1 cut(s) 113
BstAPI GCANNNNNTGC 1 cut(s) 414
BstBAI YACGTR 1 cut(s) 389
BstBI TTCGAA 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 33
BstDEI CTNAG 3 cut(s) 280, 380, 920
BstF5I GGATG 2 cut(s) 612, 740
BstHHI GCGC 1 cut(s) 444
BstMAI GTCTC 2 cut(s) 332, 859
BstMCI CGRYCG 1 cut(s) 732
BstMWI GCNNNNNNNGC 3 cut(s) 414, 507, 543
BstNI CCWGG 2 cut(s) 334, 478
BstSCI CCNGG 2 cut(s) 332, 476
BstSFI CTRYAG 1 cut(s) 316
BstV1I GCAGC 1 cut(s) 79
BstV2I GAAGAC 2 cut(s) 90, 105
BstX2I RGATCY 7 cut(s) 205, 590, 628, 848, 863, 892, 923
BstYI RGATCY 7 cut(s) 205, 590, 628, 848, 863, 892, 923
BstZ17I GTATAC 1 cut(s) 398
BtsCI GGATG 2 cut(s) 612, 740
BtsI GCAGTG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 415
BveI ACCTGC 1 cut(s) 564
Cac8I GCNNGC 1 cut(s) 33
CaiI CAGNNNCTG 1 cut(s) 333
CfoI GCGC 1 cut(s) 444
Cfr13I GGNCC 1 cut(s) 553
Csp6I GTAC 1 cut(s) 386
CspCI CAANNNNNGTGG 2 cut(s) 560, 595
CviAII CATG 3 cut(s) 358, 613, 619
CviJI RGCY 8 cut(s) 47, 70, 116, 237, 510, 516, 671, 765
CviKI_1 RGCY 8 cut(s) 47, 70, 116, 237, 510, 516, 671, 765
CviQI GTAC 1 cut(s) 386
DdeI CTNAG 3 cut(s) 280, 380, 920
Eam1104I CTCTTC 1 cut(s) 113
EarI CTCTTC 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 673
Eco32I GATATC 2 cut(s) 451, 793
Eco47I GGWCC 1 cut(s) 553
Eco57I CTGAAG 1 cut(s) 783
EcoRI GAATTC 1 cut(s) 166
EcoRII CCWGG 2 cut(s) 332, 476
EcoRV GATATC 2 cut(s) 451, 793
EcoT38I GRGCYC 1 cut(s) 673
FaeI CATG 3 cut(s) 361, 616, 622
FaiI YATR 9 cut(s) 359, 398, 440, 504, 531, 614, 620, 747, 812
FatI CATG 3 cut(s) 357, 612, 618
FbaI TGATCA 2 cut(s) 300, 609
FblI GTMKAC 1 cut(s) 397
Fnu4HI GCNGC 2 cut(s) 68, 374
FokI GGATG 2 cut(s) 619, 747
FriOI GRGCYC 1 cut(s) 673
Fsp4HI GCNGC 2 cut(s) 68, 374
FspBI CTAG 4 cut(s) 456, 632, 650, 931
FspI TGCGCA 1 cut(s) 443
GlaI GCGC 1 cut(s) 443
GluI GCNGC 2 cut(s) 68, 374
HapII CCGG 1 cut(s) 732
HhaI GCGC 1 cut(s) 444
Hin1II CATG 3 cut(s) 361, 616, 622
Hin6I GCGC 1 cut(s) 442
HinP1I GCGC 1 cut(s) 442
HincII GTYRAC 1 cut(s) 17
HindII GTYRAC 1 cut(s) 17
HinfI GANTC 3 cut(s) 191, 215, 635
HpaII CCGG 1 cut(s) 732
HphI GGTGA 1 cut(s) 259
Hpy166II GTNNAC 5 cut(s) 17, 137, 386, 398, 783
Hpy188I TCNGA 3 cut(s) 790, 802, 892
Hpy188III TCNNGA 3 cut(s) 61, 632, 666
Hpy8I GTNNAC 5 cut(s) 17, 137, 386, 398, 783
Hpy99I CGWCG 3 cut(s) 10, 77, 95
HpyAV CCTTC 2 cut(s) 44, 71
HpyCH4III ACNGT 2 cut(s) 433, 700
HpyCH4IV ACGT 1 cut(s) 388
HpyCH4V TGCA 2 cut(s) 67, 417
HpyF10VI GCNNNNNNNGC 3 cut(s) 414, 507, 543
HpyF3I CTNAG 3 cut(s) 280, 380, 920
HpySE526I ACGT 1 cut(s) 388
Hsp92II CATG 3 cut(s) 361, 616, 622
HspAI GCGC 1 cut(s) 442
Ksp22I TGATCA 2 cut(s) 300, 609
LmnI GCTCC 2 cut(s) 466, 668
Lsp1109I GCAGC 1 cut(s) 79
LweI GCATC 1 cut(s) 54
MaeI CTAG 4 cut(s) 456, 632, 650, 931
MaeII ACGT 1 cut(s) 388
MboII GAAGA 6 cut(s) 49, 95, 100, 110, 585, 872
MflI RGATCY 7 cut(s) 205, 590, 628, 848, 863, 892, 923
MhlI GDGCHC 3 cut(s) 463, 673, 698
MluCI AATT 3 cut(s) 166, 173, 715
MlyI GAGTC 1 cut(s) 200
MmeI TCCRAC 2 cut(s) 531, 584
MroXI GAANNNNTTC 1 cut(s) 567
MseI TTAA 4 cut(s) 176, 183, 404, 482
MslI CAYNNNNRTG 3 cut(s) 362, 381, 617
MspI CCGG 1 cut(s) 732
MspR9I CCNGG 2 cut(s) 334, 478
Mva1269I GAATGC 1 cut(s) 127
MvaI CCWGG 2 cut(s) 334, 478
MwoI GCNNNNNNNGC 3 cut(s) 414, 507, 543
NlaIII CATG 3 cut(s) 361, 616, 622
NlaIV GGNNCC 5 cut(s) 670, 702, 751, 865, 894
NsbI TGCGCA 1 cut(s) 443
NspV TTCGAA 1 cut(s) 111
OliI CACNNNNGTG 1 cut(s) 381
PasI CCCWGGG 1 cut(s) 333
PcsI WCGNNNNNNNCGW 3 cut(s) 78, 99, 108
PctI GAATGC 1 cut(s) 127
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 2 cut(s) 215, 635
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 333
PkrI GCNGC 2 cut(s) 69, 375
PleI GAGTC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 199
Ppu21I YACGTR 1 cut(s) 389
Psp6I CCWGG 2 cut(s) 332, 476
PspGI CCWGG 2 cut(s) 332, 476
PspN4I GGNNCC 5 cut(s) 670, 702, 751, 865, 894
PspPI GGNCC 1 cut(s) 553
PstNI CAGNNNCTG 1 cut(s) 333
PsuI RGATCY 7 cut(s) 205, 590, 628, 848, 863, 892, 923
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 1 cut(s) 387
RsaNI GTAC 1 cut(s) 386
RseI CAYNNNNRTG 3 cut(s) 362, 381, 617
SaqAI TTAA 4 cut(s) 176, 183, 404, 482
SatI GCNGC 2 cut(s) 68, 374
Sau96I GGNCC 1 cut(s) 553
SchI GAGTC 1 cut(s) 200
ScrFI CCNGG 2 cut(s) 334, 478
SduI GDGCHC 3 cut(s) 463, 673, 698
SfaNI GCATC 1 cut(s) 54
SfcI CTRYAG 1 cut(s) 316
SfuI TTCGAA 1 cut(s) 111
SinI GGWCC 1 cut(s) 553
SmiMI CAYNNNNRTG 3 cut(s) 362, 381, 617
SmlI CTYRAG 3 cut(s) 161, 511, 900
SmoI CTYRAG 3 cut(s) 161, 511, 900
Sse9I AATT 3 cut(s) 166, 173, 715
SsiI CCGC 3 cut(s) 273, 374, 867
SspMI CTAG 4 cut(s) 456, 632, 650, 931
StyD4I CCNGG 2 cut(s) 332, 476
TaaI ACNGT 2 cut(s) 433, 700
TaiI ACGT 1 cut(s) 391
TaqI TCGA 3 cut(s) 75, 90, 111
TasI AATT 3 cut(s) 166, 173, 715
TauI GCSGC 1 cut(s) 376
TfiI GAWTC 2 cut(s) 215, 635
Tru1I TTAA 4 cut(s) 176, 183, 404, 482
Tru9I TTAA 4 cut(s) 176, 183, 404, 482
TscAI CASTG 1 cut(s) 415
TseI GCWGC 1 cut(s) 67
TspDTI ATGAA 2 cut(s) 56, 111
TspRI CASTG 1 cut(s) 415
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 333
VpaK11BI GGWCC 1 cut(s) 553
XapI RAATTY 2 cut(s) 166, 715
XbaI TCTAGA 1 cut(s) 631
XmiI GTMKAC 1 cut(s) 397
XmnI GAANNNNTTC 1 cut(s) 567
XspI CTAG 4 cut(s) 456, 632, 650, 931
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.