Rh4BG015300

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000144
Physical Location & Seq
Forward (+)
2158373 .. 2164284
5912 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG015300.1

Sequence Viewer

Length: 648 bp
ATGGAGGTGATTAATGCGACTTTAGTAGTTTGCGGTTGCTTAGTGTTTGGTGCTGTGGAGATCAAAAGACTACTCCGCAGTAAACCCGGGGAGAAACTACCTGATGATATCATGGACATTGTGCCCTGGAAGAAACTACCTGACGATGTCATAGAGATGATTGTGCCACACCTGAGTGTACGTGATCGTATACGATTAAGCAGTGTTTGCAAGCCTTGGAGCTCAGCTCCTATGCGCACCGACATCCCTAGTGCTCAACATCAGCTTCCCTGGTTAATATTCCCTCAATCTTGCCATAGCTACTTGAGCTTTTTCGACCTCTGTGAAGGTAAAGTTGGCAAGTTGGACCTGCCTGAACCAGTTCAAGGAGGGTGGTTTCAAGGATCGTCAAAAGGTTGGATGATTATGATCACGGAAAAGGATCTGGATTCTACAATGTTTCTTGTGAATCCAATTTCAGGAGCCCAGCACCAACTTCCACATTTGAGCACGACTCCATCTTTCCAGAGCTTTGTAGACACAACCACATGGAAACGCTATGAGTATCTCAGAGCTTCAACATCAATTCCCGGCAAGGCGGCAACAGACTTCTGCTCAAAGCTTCAACCTCAGTTACCAGCAAAATCCTTATTCTATAAGACAGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

215

Amino Acids

24.23

Weight (kDa)

7.62

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 43 - 76 1.2e-06 F-box domain
F-box-like PF12937 43 - 75 4.4e-06 F-box-like
Beta-prop_KIB1-4 PF03478 104 - 177 2.6e-09 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 236
Acc36I ACCTGC 1 cut(s) 357
AccI GTMKAC 2 cut(s) 190, 516
AciI CCGC 3 cut(s) 33, 76, 578
AclWI GGATC 2 cut(s) 391, 429
AfaI GTAC 1 cut(s) 180
AfiI CCNNNNNNNGG 2 cut(s) 365, 458
AgsI TTSAA 4 cut(s) 365, 380, 558, 605
AjnI CCWGG 2 cut(s) 125, 269
AjuI GAANNNNNNNTTGG 2 cut(s) 318, 350
AleI CACNNNNGTG 1 cut(s) 174
AluBI AGCT 8 cut(s) 222, 227, 265, 300, 309, 510, 554, 601
AluI AGCT 8 cut(s) 222, 227, 265, 300, 309, 510, 554, 601
Alw21I GWGCWC 3 cut(s) 224, 256, 491
AlwI GGATC 2 cut(s) 391, 429
Ama87I CYCGRG 1 cut(s) 86
AseI ATTAAT 1 cut(s) 12
Asp700I GAANNNNTTC 1 cut(s) 360
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 346
AsuC2I CCSGG 3 cut(s) 87, 88, 570
AsuHPI GGTGA 1 cut(s) 19
AvaI CYCGRG 1 cut(s) 86
AvaII GGWCC 1 cut(s) 346
BaeGI GKGCMC 1 cut(s) 126
BanII GRGCYC 2 cut(s) 224, 466
Bbv12I GWGCWC 3 cut(s) 224, 256, 491
BccI CCATC 1 cut(s) 505
BciT130I CCWGG 2 cut(s) 127, 271
BclI TGATCA 1 cut(s) 408
BcnI CCSGG 3 cut(s) 87, 88, 570
BfaI CTAG 1 cut(s) 249
BfuAI ACCTGC 1 cut(s) 357
BisI GCNGC 1 cut(s) 579
BlpI GCTNAGC 1 cut(s) 223
BlsI GCNGC 1 cut(s) 580
Bme1390I CCNGG 5 cut(s) 87, 88, 127, 271, 570
Bme18I GGWCC 1 cut(s) 346
BmeT110I CYCGRG 1 cut(s) 86
BmgT120I GGNCC 1 cut(s) 346
BmiI GGNNCC 1 cut(s) 463
BmrFI CCNGG 5 cut(s) 87, 88, 127, 271, 570
BplI GAGNNNNNCTC 4 cut(s) 211, 243, 478, 510
Bpu1102I GCTNAGC 1 cut(s) 223
BpuEI CTTGAG 1 cut(s) 325
BpuMI CCSGG 3 cut(s) 87, 88, 570
BsaAI YACGTR 1 cut(s) 182
BsaBI GATNNNNATC 1 cut(s) 407
BsaJI CCNNGG 5 cut(s) 86, 87, 125, 215, 269
Bsc4I CCNNNNNNNGG 2 cut(s) 365, 458
Bse1I ACTGG 1 cut(s) 359
Bse8I GATNNNNATC 1 cut(s) 407
BseBI CCWGG 2 cut(s) 127, 271
BseDI CCNNGG 5 cut(s) 86, 87, 125, 215, 269
BseGI GGATG 2 cut(s) 243, 405
BseJI GATNNNNATC 1 cut(s) 407
BseLI CCNNNNNNNGG 2 cut(s) 365, 458
BseMII CTCAG 4 cut(s) 164, 237, 562, 623
BseNI ACTGG 1 cut(s) 359
BseSI GKGCMC 1 cut(s) 126
BseYI CCCAGC 1 cut(s) 465
BsiHKAI GWGCWC 3 cut(s) 224, 256, 491
BsiHKCI CYCGRG 1 cut(s) 86
BsiSI CCGG 2 cut(s) 87, 570
BslI CCNNNNNNNGG 2 cut(s) 365, 458
BsoBI CYCGRG 1 cut(s) 86
Bsp1286I GDGCHC 5 cut(s) 126, 224, 256, 466, 491
Bsp143I GATC 5 cut(s) 60, 184, 383, 408, 421
Bsp1720I GCTNAGC 1 cut(s) 223
BspACI CCGC 3 cut(s) 33, 76, 578
BspCNI CTCAG 4 cut(s) 165, 236, 561, 622
BspLI GGNNCC 1 cut(s) 463
BspMI ACCTGC 1 cut(s) 357
BspPI GGATC 2 cut(s) 391, 429
BsrI ACTGG 1 cut(s) 359
BssECI CCNNGG 5 cut(s) 86, 87, 125, 215, 269
BssMI GATC 5 cut(s) 60, 184, 383, 408, 421
BssNAI GTATAC 1 cut(s) 191
BssT1I CCWWGG 1 cut(s) 215
Bst1107I GTATAC 1 cut(s) 191
Bst2UI CCWGG 2 cut(s) 127, 271
BstAPI GCANNNNNTGC 1 cut(s) 207
BstBAI YACGTR 1 cut(s) 182
BstC8I GCNNGC 1 cut(s) 212
BstDEI CTNAG 5 cut(s) 40, 173, 223, 548, 609
BstF5I GGATG 2 cut(s) 243, 405
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 5 cut(s) 63, 187, 386, 411, 424
BstMBI GATC 5 cut(s) 60, 184, 383, 408, 421
BstMWI GCNNNNNNNGC 2 cut(s) 207, 306
BstNI CCWGG 2 cut(s) 127, 271
BstSCI CCNGG 5 cut(s) 85, 86, 125, 269, 568
BstSLI GKGCMC 1 cut(s) 126
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
BstZ17I GTATAC 1 cut(s) 191
BtsCI GGATG 2 cut(s) 243, 405
BtsI GCAGTG 1 cut(s) 208
BtsIMutI CAGTG 1 cut(s) 208
BveI ACCTGC 1 cut(s) 357
Cac8I GCNNGC 1 cut(s) 212
CfoI GCGC 1 cut(s) 237
Cfr13I GGNCC 1 cut(s) 346
Cfr9I CCCGGG 1 cut(s) 86
Csp6I GTAC 1 cut(s) 179
CspCI CAANNNNNGTGG 2 cut(s) 353, 388
CviAII CATG 2 cut(s) 112, 528
CviQI GTAC 1 cut(s) 179
DdeI CTNAG 5 cut(s) 40, 173, 223, 548, 609
DpnI GATC 5 cut(s) 62, 186, 385, 410, 423
DpnII GATC 5 cut(s) 60, 184, 383, 408, 421
Ecl136II GAGCTC 1 cut(s) 222
Eco130I CCWWGG 1 cut(s) 215
Eco24I GRGCYC 2 cut(s) 224, 466
Eco32I GATATC 1 cut(s) 109
Eco47I GGWCC 1 cut(s) 346
Eco53kI GAGCTC 1 cut(s) 222
Eco88I CYCGRG 1 cut(s) 86
EcoICRI GAGCTC 1 cut(s) 222
EcoRII CCWGG 2 cut(s) 125, 269
EcoRV GATATC 1 cut(s) 109
EcoT14I CCWWGG 1 cut(s) 215
EcoT38I GRGCYC 2 cut(s) 224, 466
ErhI CCWWGG 1 cut(s) 215
FaeI CATG 2 cut(s) 115, 531
FaiI YATR 9 cut(s) 113, 152, 191, 233, 297, 407, 529, 540, 636
FatI CATG 2 cut(s) 111, 527
FbaI TGATCA 1 cut(s) 408
FblI GTMKAC 2 cut(s) 190, 516
Fnu4HI GCNGC 1 cut(s) 579
FokI GGATG 2 cut(s) 230, 412
FriOI GRGCYC 2 cut(s) 224, 466
Fsp4HI GCNGC 1 cut(s) 579
FspAI RTGCGCAY 1 cut(s) 236
FspBI CTAG 1 cut(s) 249
FspI TGCGCA 1 cut(s) 236
GlaI GCGC 1 cut(s) 236
GluI GCNGC 1 cut(s) 579
GsaI CCCAGC 1 cut(s) 469
HapII CCGG 2 cut(s) 87, 570
HhaI GCGC 1 cut(s) 237
Hin1II CATG 2 cut(s) 115, 531
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HindIII AAGCTT 1 cut(s) 599
HinfI GANTC 3 cut(s) 428, 448, 493
HpaII CCGG 2 cut(s) 87, 570
HphI GGTGA 1 cut(s) 19
Hpy166II GTNNAC 4 cut(s) 83, 179, 191, 517
Hpy188I TCNGA 1 cut(s) 551
Hpy188III TCNNGA 3 cut(s) 425, 459, 505
Hpy8I GTNNAC 4 cut(s) 83, 179, 191, 517
HpyAV CCTTC 1 cut(s) 320
HpyCH4IV ACGT 1 cut(s) 181
HpyCH4V TGCA 1 cut(s) 210
HpyF10VI GCNNNNNNNGC 2 cut(s) 207, 306
HpyF3I CTNAG 5 cut(s) 40, 173, 223, 548, 609
HpySE526I ACGT 1 cut(s) 181
Hsp92II CATG 2 cut(s) 115, 531
HspAI GCGC 1 cut(s) 235
Ksp22I TGATCA 1 cut(s) 408
Kzo9I GATC 5 cut(s) 60, 184, 383, 408, 421
LmnI GCTCC 3 cut(s) 219, 232, 461
MaeI CTAG 1 cut(s) 249
MaeII ACGT 1 cut(s) 181
MaeIII GTNAC 1 cut(s) 612
MalI GATC 5 cut(s) 62, 186, 385, 410, 423
MboI GATC 5 cut(s) 60, 184, 383, 408, 421
MboII GAAGA 1 cut(s) 142
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 5 cut(s) 126, 224, 256, 466, 491
MluCI AATT 2 cut(s) 453, 564
MlyI GAGTC 1 cut(s) 487
MmeI TCCRAC 2 cut(s) 324, 377
MnlI CCTC 4 cut(s) 294, 329, 362, 618
MroXI GAANNNNTTC 1 cut(s) 360
MseI TTAA 3 cut(s) 12, 197, 275
MslI CAYNNNNRTG 2 cut(s) 155, 174
MspI CCGG 2 cut(s) 87, 570
MspR9I CCNGG 5 cut(s) 87, 88, 127, 271, 570
MvaI CCWGG 2 cut(s) 127, 271
MwoI GCNNNNNNNGC 2 cut(s) 207, 306
NciI CCSGG 3 cut(s) 87, 88, 570
NdeII GATC 5 cut(s) 60, 184, 383, 408, 421
NlaIII CATG 2 cut(s) 115, 531
NlaIV GGNNCC 1 cut(s) 463
NsbI TGCGCA 1 cut(s) 236
OliI CACNNNNGTG 1 cut(s) 174
PdmI GAANNNNTTC 1 cut(s) 360
PfeI GAWTC 2 cut(s) 428, 448
PflFI GACNNNGTC 1 cut(s) 146
PkrI GCNGC 1 cut(s) 580
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
Ppu21I YACGTR 1 cut(s) 182
PshBI ATTAAT 1 cut(s) 12
Psp124BI GAGCTC 1 cut(s) 224
Psp6I CCWGG 2 cut(s) 125, 269
PspFI CCCAGC 1 cut(s) 465
PspGI CCWGG 2 cut(s) 125, 269
PspN4I GGNNCC 1 cut(s) 463
PspPI GGNCC 1 cut(s) 346
PsuI RGATCY 1 cut(s) 421
PsyI GACNNNGTC 1 cut(s) 146
RsaI GTAC 1 cut(s) 180
RsaNI GTAC 1 cut(s) 179
RseI CAYNNNNRTG 2 cut(s) 155, 174
SacI GAGCTC 1 cut(s) 224
SaqAI TTAA 3 cut(s) 12, 197, 275
SatI GCNGC 1 cut(s) 579
Sau3AI GATC 5 cut(s) 60, 184, 383, 408, 421
Sau96I GGNCC 1 cut(s) 346
SchI GAGTC 1 cut(s) 487
ScrFI CCNGG 5 cut(s) 87, 88, 127, 271, 570
SduI GDGCHC 5 cut(s) 126, 224, 256, 466, 491
SinI GGWCC 1 cut(s) 346
SmaI CCCGGG 1 cut(s) 88
SmiMI CAYNNNNRTG 2 cut(s) 155, 174
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
Sse9I AATT 2 cut(s) 453, 564
SsiI CCGC 3 cut(s) 33, 76, 578
SspI AATATT 1 cut(s) 279
SspMI CTAG 1 cut(s) 249
SstI GAGCTC 1 cut(s) 224
StyD4I CCNGG 5 cut(s) 85, 86, 125, 269, 568
StyI CCWWGG 1 cut(s) 215
TaiI ACGT 1 cut(s) 184
TaqI TCGA 1 cut(s) 315
TasI AATT 2 cut(s) 453, 564
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 2 cut(s) 428, 448
Tru1I TTAA 3 cut(s) 12, 197, 275
Tru9I TTAA 3 cut(s) 12, 197, 275
TscAI CASTG 1 cut(s) 208
TspGWI ACGGA 1 cut(s) 428
TspMI CCCGGG 1 cut(s) 86
TspRI CASTG 1 cut(s) 208
Tth111I GACNNNGTC 1 cut(s) 146
VpaK11BI GGWCC 1 cut(s) 346
VspI ATTAAT 1 cut(s) 12
XmaI CCCGGG 1 cut(s) 86
XmiI GTMKAC 2 cut(s) 190, 516
XmnI GAANNNNTTC 1 cut(s) 360
XspI CTAG 1 cut(s) 249
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.