Rh6DG082300

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000144
Physical Location & Seq
Reverse (-)
9594540 .. 9595982
1443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG082300.1

Sequence Viewer

Length: 900 bp
ATGGTCATGCTCTCTATATATTTGTCTTACAGTAACCTGGGAAACTACCTCAGCTGGTTCACCAGGTCTGTTTCCTTCAAGCAATTACATGAACACAAAATATCTGCAGGCAAGAGAAAACAACTCGAGAAGTACTGCAACGACAACCAAATTACTTGTCATGGGTCAGTCGGGTGCTGGCAGACACCACCACAAGACATCATGGAGGAGATTCTACAGAAAATGGGTTTAATTGCTCGTACACGCTTAGCCAAGGTCTGCATGTCTTGGAGCTCACTTGTTATGAGAGCTGACATCCGCAGTGCTCCGCAACTGCCATGGTTAGTACACCCTCAAACCCCAAACACCAACTACCTAACCTTTTCCAGCCTCTCTGAGGGTGAAGTTGTGAACTTGGAGCTCCCAAACCCAAAGCGATTCGGAGGACAAGGCGAATTCAAGTTTCGAGCGGCTTCTCAAGAATTAACAACAATTAGCGAAAAGGAAATCTTGTTGATCCATCAAATGCTCGATTGTTTAATGCTCAACTGTGTTGATGATAATGAGTGGGAAATCGAATATCAGAGGACAAGAGGGTTTATAGTATACAAGATTGACCTCGAGTGTGGCAACTTTCGTGAGGTACAAAATTTGGGGGACCAAATAATATTCTTGGCAGAGCATAGCTCTTCCTTGTCCTTTCCAGCAAGTGATTTCAAACTACAAGGAAATTGCATTTATTTTGCAACAAGCGGATATGCAGCTATCCGCCTGCCTCGTACGACACCTCTTGGACCAAATATATCTCGTGAAATCGGAATCTTTAGTTTAGATGACGGAAAAGTTAAGCGACCCTTTCCAAGTCTTGCTTTTATTGGCTATTCATCTTGTCAACTGAGTTGGTTCAGTCCGAGTCTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

299

Amino Acids

34.02

Weight (kDa)

6.99

Isoelectric Point (pI)

45.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 134 - 268 5.4e-15 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 449
AccI GTMKAC 1 cut(s) 585
AciI CCGC 5 cut(s) 298, 308, 449, 732, 748
AclWI GGATC 1 cut(s) 490
AcsI RAATTY 2 cut(s) 434, 628
AfaI GTAC 5 cut(s) 134, 241, 327, 624, 760
AfiI CCNNNNNNNGG 1 cut(s) 376
AgsI TTSAA 3 cut(s) 79, 439, 697
AjnI CCWGG 2 cut(s) 36, 62
AluBI AGCT 6 cut(s) 54, 273, 290, 400, 666, 743
AluI AGCT 6 cut(s) 54, 273, 290, 400, 666, 743
Alw21I GWGCWC 3 cut(s) 275, 307, 402
AlwI GGATC 1 cut(s) 490
Ama87I CYCGRG 2 cut(s) 125, 599
ApeKI GCWGC 1 cut(s) 740
ApoI RAATTY 2 cut(s) 434, 628
ArsI GACNNNNNNTTYG 2 cut(s) 142, 174
AspS9I GGNCC 2 cut(s) 637, 773
AsuHPI GGTGA 2 cut(s) 52, 392
AvaI CYCGRG 2 cut(s) 125, 599
AvaII GGWCC 2 cut(s) 637, 773
BanII GRGCYC 2 cut(s) 275, 402
BauI CACGAG 1 cut(s) 786
Bbv12I GWGCWC 3 cut(s) 275, 307, 402
BbvCI CCTCAGC 1 cut(s) 50
BbvI GCAGC 1 cut(s) 752
BccI CCATC 1 cut(s) 507
BciT130I CCWGG 2 cut(s) 38, 64
BfmI CTRYAG 2 cut(s) 105, 215
BisI GCNGC 2 cut(s) 450, 741
BlpI GCTNAGC 1 cut(s) 247
BlsI GCNGC 2 cut(s) 451, 742
BmcAI AGTACT 1 cut(s) 134
Bme1390I CCNGG 2 cut(s) 38, 64
Bme18I GGWCC 2 cut(s) 637, 773
BmeT110I CYCGRG 2 cut(s) 125, 599
BmgT120I GGNCC 2 cut(s) 637, 773
BmiI GGNNCC 1 cut(s) 638
BmrFI CCNGG 2 cut(s) 38, 64
BplI GAGNNNNNCTC 2 cut(s) 650, 682
Bpu10I CCTNAGC 1 cut(s) 50
Bpu1102I GCTNAGC 1 cut(s) 247
BpuEI CTTGAG 1 cut(s) 441
BsaJI CCNNGG 3 cut(s) 37, 252, 317
Bsc4I CCNNNNNNNGG 1 cut(s) 376
BseBI CCWGG 2 cut(s) 38, 64
BseDI CCNNGG 3 cut(s) 37, 252, 317
BseGI GGATG 1 cut(s) 294
BseLI CCNNNNNNNGG 1 cut(s) 376
BseMII CTCAG 3 cut(s) 64, 366, 866
BseRI GAGGAG 1 cut(s) 221
BseXI GCAGC 1 cut(s) 752
BsiHKAI GWGCWC 3 cut(s) 275, 307, 402
BsiHKCI CYCGRG 2 cut(s) 125, 599
BsiWI CGTACG 1 cut(s) 758
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 1 cut(s) 376
BsmFI GGGAC 1 cut(s) 650
BsoBI CYCGRG 2 cut(s) 125, 599
Bsp1286I GDGCHC 3 cut(s) 275, 307, 402
Bsp143I GATC 1 cut(s) 495
Bsp1720I GCTNAGC 1 cut(s) 247
Bsp19I CCATGG 1 cut(s) 317
BspACI CCGC 5 cut(s) 298, 308, 449, 732, 748
BspCNI CTCAG 3 cut(s) 63, 367, 867
BspLI GGNNCC 1 cut(s) 638
BspMAI CTGCAG 1 cut(s) 109
BspPI GGATC 1 cut(s) 490
BspQI GCTCTTC 1 cut(s) 673
BsrBI CCGCTC 1 cut(s) 449
BssECI CCNNGG 3 cut(s) 37, 252, 317
BssMI GATC 1 cut(s) 495
BssNAI GTATAC 1 cut(s) 586
BssSI CACGAG 1 cut(s) 786
BssT1I CCWWGG 2 cut(s) 252, 317
Bst1107I GTATAC 1 cut(s) 586
Bst2BI CACGAG 1 cut(s) 786
Bst2UI CCWGG 2 cut(s) 38, 64
Bst4CI ACNGT 2 cut(s) 32, 530
Bst6I CTCTTC 1 cut(s) 673
BstC8I GCNNGC 3 cut(s) 109, 179, 752
BstDEI CTNAG 4 cut(s) 50, 247, 375, 875
BstDSI CCRYGG 1 cut(s) 317
BstENI CCTNNNNNAGG 1 cut(s) 374
BstF5I GGATG 1 cut(s) 294
BstKTI GATC 1 cut(s) 498
BstMBI GATC 1 cut(s) 495
BstNI CCWGG 2 cut(s) 38, 64
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 2 cut(s) 36, 62
BstSFI CTRYAG 2 cut(s) 105, 215
BstV1I GCAGC 1 cut(s) 752
BstZ17I GTATAC 1 cut(s) 586
BtgI CCRYGG 1 cut(s) 317
BtsCI GGATG 1 cut(s) 294
BtsI GCAGTG 1 cut(s) 307
BtsIMutI CAGTG 1 cut(s) 307
Cac8I GCNNGC 3 cut(s) 109, 179, 752
Cfr13I GGNCC 2 cut(s) 637, 773
CsiI ACCWGGT 1 cut(s) 62
Csp6I GTAC 5 cut(s) 133, 240, 326, 623, 759
CviAII CATG 6 cut(s) 7, 89, 161, 202, 262, 318
CviQI GTAC 5 cut(s) 133, 240, 326, 623, 759
DdeI CTNAG 4 cut(s) 50, 247, 375, 875
DpnI GATC 1 cut(s) 497
DpnII GATC 1 cut(s) 495
Eam1104I CTCTTC 1 cut(s) 673
EarI CTCTTC 1 cut(s) 673
EciI GGCGGA 1 cut(s) 737
Ecl136II GAGCTC 2 cut(s) 273, 400
Eco130I CCWWGG 2 cut(s) 252, 317
Eco24I GRGCYC 2 cut(s) 275, 402
Eco47I GGWCC 2 cut(s) 637, 773
Eco53kI GAGCTC 2 cut(s) 273, 400
Eco88I CYCGRG 2 cut(s) 125, 599
EcoICRI GAGCTC 2 cut(s) 273, 400
EcoNI CCTNNNNNAGG 1 cut(s) 374
EcoRI GAATTC 1 cut(s) 434
EcoRII CCWGG 2 cut(s) 36, 62
EcoT14I CCWWGG 2 cut(s) 252, 317
EcoT38I GRGCYC 2 cut(s) 275, 402
ErhI CCWWGG 2 cut(s) 252, 317
FaeI CATG 6 cut(s) 10, 92, 164, 205, 265, 321
FalI AAGNNNNNCTT 6 cut(s) 473, 505, 818, 850, 832, 864
FaqI GGGAC 1 cut(s) 650
FatI CATG 6 cut(s) 6, 88, 160, 201, 261, 317
FblI GTMKAC 1 cut(s) 585
Fnu4HI GCNGC 2 cut(s) 450, 741
FokI GGATG 1 cut(s) 281
FriOI GRGCYC 2 cut(s) 275, 402
Fsp4HI GCNGC 2 cut(s) 450, 741
GluI GCNGC 2 cut(s) 450, 741
Hin1II CATG 6 cut(s) 10, 92, 164, 205, 265, 321
HincII GTYRAC 1 cut(s) 872
HindII GTYRAC 1 cut(s) 872
HinfI GANTC 4 cut(s) 211, 417, 798, 892
HphI GGTGA 2 cut(s) 52, 392
Hpy166II GTNNAC 6 cut(s) 60, 242, 328, 391, 586, 872
Hpy188I TCNGA 5 cut(s) 376, 422, 564, 797, 891
Hpy188III TCNNGA 4 cut(s) 127, 458, 617, 788
Hpy8I GTNNAC 6 cut(s) 60, 242, 328, 391, 586, 872
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 2 cut(s) 32, 530
HpyCH4V TGCA 6 cut(s) 107, 138, 261, 714, 725, 740
HpyF3I CTNAG 4 cut(s) 50, 247, 375, 875
Hsp92II CATG 6 cut(s) 10, 92, 164, 205, 265, 321
Kzo9I GATC 1 cut(s) 495
LguI GCTCTTC 1 cut(s) 673
LmnI GCTCC 4 cut(s) 270, 310, 397, 405
Lsp1109I GCAGC 1 cut(s) 752
MabI ACCWGGT 1 cut(s) 62
MaeIII GTNAC 1 cut(s) 32
MalI GATC 1 cut(s) 497
MbiI CCGCTC 1 cut(s) 449
MboI GATC 1 cut(s) 495
MboII GAAGA 1 cut(s) 660
MhlI GDGCHC 3 cut(s) 275, 307, 402
MluCI AATT 8 cut(s) 83, 150, 231, 434, 461, 471, 628, 709
MseI TTAA 4 cut(s) 230, 464, 518, 825
MspA1I CMGCKG 1 cut(s) 54
MspR9I CCNGG 2 cut(s) 38, 64
MvaI CCWGG 2 cut(s) 38, 64
NcoI CCATGG 1 cut(s) 317
NdeII GATC 1 cut(s) 495
NlaIII CATG 6 cut(s) 10, 92, 164, 205, 265, 321
NlaIV GGNNCC 1 cut(s) 638
NspI RCATGY 1 cut(s) 265
PaeR7I CTCGAG 2 cut(s) 125, 599
PciSI GCTCTTC 1 cut(s) 673
PfeI GAWTC 3 cut(s) 211, 417, 798
Pfl23II CGTACG 1 cut(s) 758
PkrI GCNGC 2 cut(s) 451, 742
Psp124BI GAGCTC 2 cut(s) 275, 402
Psp6I CCWGG 2 cut(s) 36, 62
PspGI CCWGG 2 cut(s) 36, 62
PspLI CGTACG 1 cut(s) 758
PspN4I GGNNCC 1 cut(s) 638
PspPI GGNCC 2 cut(s) 637, 773
PspXI VCTCGAGB 1 cut(s) 599
PstI CTGCAG 1 cut(s) 109
PvuII CAGCTG 1 cut(s) 54
RsaI GTAC 5 cut(s) 134, 241, 327, 624, 760
RsaNI GTAC 5 cut(s) 133, 240, 326, 623, 759
SacI GAGCTC 2 cut(s) 275, 402
SapI GCTCTTC 1 cut(s) 673
SaqAI TTAA 4 cut(s) 230, 464, 518, 825
SatI GCNGC 2 cut(s) 450, 741
Sau3AI GATC 1 cut(s) 495
Sau96I GGNCC 2 cut(s) 637, 773
ScaI AGTACT 1 cut(s) 134
ScrFI CCNGG 2 cut(s) 38, 64
SduI GDGCHC 3 cut(s) 275, 307, 402
SexAI ACCWGGT 1 cut(s) 62
SfcI CTRYAG 2 cut(s) 105, 215
Sfr274I CTCGAG 2 cut(s) 125, 599
SinI GGWCC 2 cut(s) 637, 773
SlaI CTCGAG 2 cut(s) 125, 599
SmlI CTYRAG 3 cut(s) 125, 456, 599
SmoI CTYRAG 3 cut(s) 125, 456, 599
Sse9I AATT 8 cut(s) 83, 150, 231, 434, 461, 471, 628, 709
SsiI CCGC 5 cut(s) 298, 308, 449, 732, 748
SspI AATATT 1 cut(s) 648
SstI GAGCTC 2 cut(s) 275, 402
StyD4I CCNGG 2 cut(s) 36, 62
StyI CCWWGG 2 cut(s) 252, 317
TaaI ACNGT 2 cut(s) 32, 530
TaqI TCGA 5 cut(s) 126, 445, 510, 555, 600
TasI AATT 8 cut(s) 83, 150, 231, 434, 461, 471, 628, 709
TatI WGTACW 2 cut(s) 132, 325
TauI GCSGC 1 cut(s) 452
TfiI GAWTC 3 cut(s) 211, 417, 798
Tru1I TTAA 4 cut(s) 230, 464, 518, 825
Tru9I TTAA 4 cut(s) 230, 464, 518, 825
TscAI CASTG 1 cut(s) 307
TseI GCWGC 1 cut(s) 740
TspDTI ATGAA 2 cut(s) 105, 852
TspGWI ACGGA 1 cut(s) 831
TspRI CASTG 1 cut(s) 307
VpaK11BI GGWCC 2 cut(s) 637, 773
XagI CCTNNNNNAGG 1 cut(s) 374
XapI RAATTY 2 cut(s) 434, 628
XceI RCATGY 1 cut(s) 265
XhoI CTCGAG 2 cut(s) 125, 599
XmiI GTMKAC 1 cut(s) 585
ZrmI AGTACT 1 cut(s) 134
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.