Rroxscaffold_5G00356450

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000144
Physical Location & Seq
Forward (+)
35757254 .. 35760566
3313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00356450.1

Sequence Viewer

Length: 411 bp
ATGTGGGATCCTGTTGCGTACGCTCTTGGGTTCACCGAGTATGGTGCACAAAAGAGGATAGACATTGACTCCTACTTAGACTTACTATTACTCGATTTCATAATGATTGGTTTTATCCGGCCCGGACATCTCATAATGTATGCAGCAGTTCATAATAGTGGCGAACTGGCTTTGTGTAGACCTAGAGACAAAAGCTGGAGTGTCTACCAAGTATTTGAAGAAAATGAAAATCTTGTTGAGAGAGTCAACAGAATATTGTTTTCCACCACCGGCGTGCTATATGCCTTGGTTACGTGTAACCGCAATGGTTGTGGTGTTGATGCAGACCGCTCTTTAAAGTTCGGAGATGAGGCGGTGAAATTGAAGCTGATCTATGACATAGAGGAGTGGTGGATTGATGCGAGGGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

136

Amino Acids

15.61

Weight (kDa)

4.85

Isoelectric Point (pI)

29.71

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 330
AccI GTMKAC 2 cut(s) 178, 204
AciI CCGC 3 cut(s) 301, 328, 353
AclWI GGATC 2 cut(s) 2, 15
AfaI GTAC 1 cut(s) 20
AflIII ACRYGT 1 cut(s) 293
AgsI TTSAA 2 cut(s) 218, 364
AleI CACNNNNGTG 1 cut(s) 272
AluBI AGCT 2 cut(s) 195, 367
AluI AGCT 2 cut(s) 195, 367
Alw21I GWGCWC 1 cut(s) 49
Alw26I GTCTC 1 cut(s) 180
Alw44I GTGCAC 1 cut(s) 45
AlwI GGATC 2 cut(s) 2, 15
AoxI GGCC 1 cut(s) 119
ApaLI GTGCAC 1 cut(s) 45
ApeKI GCWGC 1 cut(s) 143
AspS9I GGNCC 1 cut(s) 120
AsuC2I CCSGG 1 cut(s) 123
AsuHPI GGTGA 2 cut(s) 25, 367
BaeGI GKGCMC 1 cut(s) 49
BamHI GGATCC 1 cut(s) 7
Bbv12I GWGCWC 1 cut(s) 49
BbvI GCAGC 1 cut(s) 155
BcgI CGANNNNNNTGC 2 cut(s) 26, 60
BcnI CCSGG 1 cut(s) 123
BcoDI GTCTC 1 cut(s) 180
BfaI CTAG 1 cut(s) 183
BisI GCNGC 1 cut(s) 144
BlsI GCNGC 1 cut(s) 145
Bme1390I CCNGG 1 cut(s) 123
BmgT120I GGNCC 1 cut(s) 120
BmiI GGNNCC 1 cut(s) 9
BmrFI CCNGG 1 cut(s) 123
BmsI GCATC 2 cut(s) 310, 388
BpmI CTGGAG 1 cut(s) 217
BpuMI CCSGG 1 cut(s) 123
BsaAI YACGTR 1 cut(s) 294
BsaJI CCNNGG 1 cut(s) 285
BsaXI ACNNNNNCTCC 2 cut(s) 53, 83
Bse118I RCCGGY 1 cut(s) 269
Bse1I ACTGG 1 cut(s) 171
Bse3DI GCAATG 1 cut(s) 310
BseDI CCNNGG 1 cut(s) 285
BseMI GCAATG 1 cut(s) 310
BseNI ACTGG 1 cut(s) 171
BseRI GAGGAG 1 cut(s) 398
BseSI GKGCMC 1 cut(s) 49
BseXI GCAGC 1 cut(s) 155
BshFI GGCC 1 cut(s) 121
BsiHKAI GWGCWC 1 cut(s) 49
BsiSI CCGG 3 cut(s) 118, 123, 270
BsiWI CGTACG 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 180
BsnI GGCC 1 cut(s) 121
Bsp1286I GDGCHC 1 cut(s) 49
Bsp143I GATC 2 cut(s) 7, 369
BspACI CCGC 3 cut(s) 301, 328, 353
BspANI GGCC 1 cut(s) 121
BspLI GGNNCC 1 cut(s) 9
BspPI GGATC 2 cut(s) 2, 15
BsrBI CCGCTC 1 cut(s) 330
BsrDI GCAATG 1 cut(s) 310
BsrFI RCCGGY 1 cut(s) 269
BsrI ACTGG 1 cut(s) 171
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 1 cut(s) 285
BssMI GATC 2 cut(s) 7, 369
BssT1I CCWWGG 1 cut(s) 285
BstBAI YACGTR 1 cut(s) 294
BstC8I GCNNGC 1 cut(s) 275
BstDEI CTNAG 1 cut(s) 76
BstKTI GATC 2 cut(s) 10, 372
BstMAI GTCTC 1 cut(s) 180
BstMBI GATC 2 cut(s) 7, 369
BstSCI CCNGG 1 cut(s) 121
BstSLI GKGCMC 1 cut(s) 49
BstV1I GCAGC 1 cut(s) 155
BstX2I RGATCY 1 cut(s) 7
BstYI RGATCY 1 cut(s) 7
BsuRI GGCC 1 cut(s) 121
Cac8I GCNNGC 1 cut(s) 275
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 120
Csp6I GTAC 1 cut(s) 19
CspCI CAANNNNNGTGG 2 cut(s) 292, 327
CviJI RGCY 4 cut(s) 121, 170, 195, 367
CviKI_1 RGCY 4 cut(s) 121, 170, 195, 367
CviQI GTAC 1 cut(s) 19
DdeI CTNAG 1 cut(s) 76
DpnI GATC 2 cut(s) 9, 371
DpnII GATC 2 cut(s) 7, 369
DraI TTTAAA 1 cut(s) 336
Eco130I CCWWGG 1 cut(s) 285
EcoT14I CCWWGG 1 cut(s) 285
ErhI CCWWGG 1 cut(s) 285
FaiI YATR 9 cut(s) 42, 101, 134, 141, 153, 280, 282, 375, 380
FblI GTMKAC 2 cut(s) 178, 204
Fnu4HI GCNGC 1 cut(s) 144
Fsp4HI GCNGC 1 cut(s) 144
FspBI CTAG 1 cut(s) 183
GluI GCNGC 1 cut(s) 144
GsuI CTGGAG 1 cut(s) 217
HaeIII GGCC 1 cut(s) 121
HapII CCGG 3 cut(s) 118, 123, 270
HincII GTYRAC 1 cut(s) 247
HindII GTYRAC 1 cut(s) 247
HinfI GANTC 2 cut(s) 68, 243
HpaII CCGG 3 cut(s) 118, 123, 270
HphI GGTGA 2 cut(s) 25, 367
Hpy166II GTNNAC 5 cut(s) 33, 47, 179, 205, 247
Hpy188I TCNGA 1 cut(s) 344
Hpy8I GTNNAC 5 cut(s) 33, 47, 179, 205, 247
HpyCH4IV ACGT 1 cut(s) 293
HpyCH4V TGCA 3 cut(s) 47, 143, 323
HpyF3I CTNAG 1 cut(s) 76
HpySE526I ACGT 1 cut(s) 293
Kzo9I GATC 2 cut(s) 7, 369
LpnPI CCDG 6 cut(s) 24, 131, 136, 152, 181, 283
Lsp1109I GCAGC 1 cut(s) 155
LweI GCATC 2 cut(s) 310, 388
MaeI CTAG 1 cut(s) 183
MaeII ACGT 1 cut(s) 293
MaeIII GTNAC 2 cut(s) 289, 296
MalI GATC 2 cut(s) 9, 371
MbiI CCGCTC 1 cut(s) 330
MboI GATC 2 cut(s) 7, 369
MboII GAAGA 1 cut(s) 230
MflI RGATCY 1 cut(s) 7
MhlI GDGCHC 1 cut(s) 49
MluCI AATT 1 cut(s) 359
MlyI GAGTC 2 cut(s) 62, 252
MnlI CCTC 4 cut(s) 48, 343, 376, 396
MseI TTAA 1 cut(s) 335
MslI CAYNNNNRTG 2 cut(s) 156, 272
MspI CCGG 3 cut(s) 118, 123, 270
MspR9I CCNGG 1 cut(s) 123
NciI CCSGG 1 cut(s) 123
NdeII GATC 2 cut(s) 7, 369
NlaIV GGNNCC 1 cut(s) 9
OliI CACNNNNGTG 1 cut(s) 272
Pfl23II CGTACG 1 cut(s) 18
PkrI GCNGC 1 cut(s) 145
PleI GAGTC 2 cut(s) 62, 251
PpsI GAGTC 2 cut(s) 62, 251
Ppu21I YACGTR 1 cut(s) 294
PspLI CGTACG 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 9
PspPI GGNCC 1 cut(s) 120
PsuI RGATCY 1 cut(s) 7
RsaI GTAC 1 cut(s) 20
RsaNI GTAC 1 cut(s) 19
RseI CAYNNNNRTG 2 cut(s) 156, 272
SaqAI TTAA 1 cut(s) 335
SatI GCNGC 1 cut(s) 144
Sau3AI GATC 2 cut(s) 7, 369
Sau96I GGNCC 1 cut(s) 120
SchI GAGTC 2 cut(s) 62, 252
ScrFI CCNGG 1 cut(s) 123
SduI GDGCHC 1 cut(s) 49
SetI ASST 4 cut(s) 184, 197, 296, 369
SfaNI GCATC 2 cut(s) 310, 388
SgrAI CRCCGGYG 1 cut(s) 269
SmiMI CAYNNNNRTG 2 cut(s) 156, 272
Sse9I AATT 1 cut(s) 359
SsiI CCGC 3 cut(s) 301, 328, 353
SspI AATATT 1 cut(s) 255
SspMI CTAG 1 cut(s) 183
StyD4I CCNGG 1 cut(s) 121
StyI CCWWGG 1 cut(s) 285
TaiI ACGT 1 cut(s) 296
TaqI TCGA 1 cut(s) 93
TasI AATT 1 cut(s) 359
Tru1I TTAA 1 cut(s) 335
Tru9I TTAA 1 cut(s) 335
TseI GCWGC 1 cut(s) 143
TspDTI ATGAA 3 cut(s) 88, 140, 240
VneI GTGCAC 1 cut(s) 45
XmiI GTMKAC 2 cut(s) 178, 204
XspI CTAG 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.