MD17G1018900.v1.1

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0000144
Physical Location & Seq
Forward (+)
1477564 .. 1481630
4067 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1018900.v1.1.491

Sequence Viewer

Length: 738 bp
ATGAGGACTTCGATCTTCCCAGCGAGGAGAAGGAAATTGGCAGGCAGGGCCTGGAGAAGAAGAAGAAGAAGAAGCGAAGGTTCACTGAAAACTTTGGTGTCATGGCGTGAAGTTCTGGAACTGCTACAGTTGTGGAGGGATGTTGGCAGACACCCTTTGGATTTCAGGGAGAATATCGTACGTCGCCTCGACTTGCTTGATCTGATTCGCTTAAGCGTCCTCTGCAAGTACTGGAGTTCAATTGCTATGCGAAGGGACATCCGCAGTACTGCTCCACCATTTCCCTGGTTAGTACTCGCTCAACGTAGTTCCGACTACTTAGAGTTCTCCATCCCATCTGAGGGTAAAGTTATCAAGATAGATTTGCCTAAGCGAAATCAAGGGCGGTTTCATAGTTCCTCCAAAGGTTGGTTGAATATGGTGAAGGGAAAATCCATATTTCTACTAAACCCTATTTCAGGAGACAAGCAAGAACTTCCTTCCTTGAGAAATATCCCATCTCTCCACAGAATGTACGGTGGAAAACCTGCTCAATTTCTTCATGAAATTGCACTATCCGGTTCAAATACCTCAGAATGTACGGTGGCAGCGATTTTTTCTTCTAAGGAAATTGGTGTGTGCAGGCCGGGAGAAAAAAGTTGGAGCATCTTCTCGATGTTGGATGATAGAAAAGGTTCTCCTTTACAACTATTGTTTTCGTCTTGTGGCATGCTATACGTATTGGTAAAAAAACAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

246

Amino Acids

28.23

Weight (kDa)

10.71

Isoelectric Point (pI)

69.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 52 - 87 4.6e-06 F-box domain
Beta-prop_KIB1-4 PF03478 109 - 225 4.7e-17 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 535
AccB7I CCANNNNNTGG 1 cut(s) 408
AciI CCGC 2 cut(s) 262, 385
AfaI GTAC 6 cut(s) 180, 230, 268, 294, 515, 580
AfiI CCNNNNNNNGG 4 cut(s) 340, 341, 408, 458
AflII CTTAAG 1 cut(s) 211
AgsI TTSAA 3 cut(s) 240, 415, 564
AjnI CCWGG 2 cut(s) 50, 284
Alw26I GTCTC 1 cut(s) 456
AlwNI CAGNNNCTG 1 cut(s) 51
AoxI GGCC 2 cut(s) 48, 623
ApeKI GCWGC 1 cut(s) 587
Asp700I GAANNNNTTC 1 cut(s) 673
AspS9I GGNCC 1 cut(s) 48
AsuC2I CCSGG 1 cut(s) 627
AsuHPI GGTGA 1 cut(s) 433
BbvI GCAGC 1 cut(s) 599
BccI CCATC 3 cut(s) 338, 343, 505
BciT130I CCWGG 2 cut(s) 52, 286
BcnI CCSGG 1 cut(s) 627
BcoDI GTCTC 1 cut(s) 456
BfmI CTRYAG 1 cut(s) 125
BfrI CTTAAG 1 cut(s) 211
BfuAI ACCTGC 1 cut(s) 535
BisI GCNGC 1 cut(s) 588
BlsI GCNGC 1 cut(s) 589
BmcAI AGTACT 3 cut(s) 230, 268, 294
Bme1390I CCNGG 3 cut(s) 52, 286, 627
BmgT120I GGNCC 1 cut(s) 48
BmrFI CCNGG 3 cut(s) 52, 286, 627
BmsI GCATC 1 cut(s) 654
BpmI CTGGAG 2 cut(s) 73, 253
Bpu10I CCTNAGC 1 cut(s) 369
BpuEI CTTGAG 1 cut(s) 505
BpuMI CCSGG 1 cut(s) 627
BsaAI YACGTR 1 cut(s) 718
BsaJI CCNNGG 1 cut(s) 284
BsaWI WCCGGW 1 cut(s) 557
Bsc4I CCNNNNNNNGG 4 cut(s) 340, 341, 408, 458
Bse1I ACTGG 1 cut(s) 236
BseBI CCWGG 2 cut(s) 52, 286
BseDI CCNNGG 1 cut(s) 284
BseGI GGATG 4 cut(s) 145, 258, 330, 667
BseLI CCNNNNNNNGG 4 cut(s) 340, 341, 408, 458
BseMII CTCAG 2 cut(s) 330, 585
BseNI ACTGG 1 cut(s) 236
BseRI GAGGAG 1 cut(s) 40
BseXI GCAGC 1 cut(s) 599
BseYI CCCAGC 1 cut(s) 19
BsgI GTGCAG 1 cut(s) 640
BshFI GGCC 2 cut(s) 50, 625
BsiSI CCGG 2 cut(s) 558, 626
BsiWI CGTACG 1 cut(s) 178
BslFI GGGAC 1 cut(s) 269
BslI CCNNNNNNNGG 4 cut(s) 340, 341, 408, 458
BsmAI GTCTC 1 cut(s) 456
BsmFI GGGAC 1 cut(s) 269
BsnI GGCC 2 cut(s) 50, 625
Bsp143I GATC 2 cut(s) 12, 199
BspACI CCGC 2 cut(s) 262, 385
BspANI GGCC 2 cut(s) 50, 625
BspCNI CTCAG 2 cut(s) 331, 584
BspHI TCATGA 1 cut(s) 541
BspMI ACCTGC 1 cut(s) 535
BspTI CTTAAG 1 cut(s) 211
BsrI ACTGG 1 cut(s) 236
BssECI CCNNGG 1 cut(s) 284
BssMI GATC 2 cut(s) 12, 199
Bst2UI CCWGG 2 cut(s) 52, 286
Bst4CI ACNGT 3 cut(s) 129, 518, 583
BstAFI CTTAAG 1 cut(s) 211
BstBAI YACGTR 1 cut(s) 718
BstC8I GCNNGC 3 cut(s) 43, 623, 710
BstDEI CTNAG 5 cut(s) 319, 339, 369, 571, 603
BstENI CCTNNNNNAGG 1 cut(s) 456
BstF5I GGATG 4 cut(s) 145, 258, 330, 667
BstKTI GATC 2 cut(s) 15, 202
BstMAI GTCTC 1 cut(s) 456
BstMBI GATC 2 cut(s) 12, 199
BstMWI GCNNNNNNNGC 2 cut(s) 47, 222
BstNI CCWGG 2 cut(s) 52, 286
BstNSI RCATGY 1 cut(s) 712
BstSCI CCNGG 3 cut(s) 50, 284, 625
BstSFI CTRYAG 1 cut(s) 125
BstSNI TACGTA 1 cut(s) 718
BstV1I GCAGC 1 cut(s) 599
BstXI CCANNNNNNTGG 1 cut(s) 285
BsuRI GGCC 2 cut(s) 50, 625
BtsCI GGATG 4 cut(s) 145, 258, 330, 667
BtsIMutI CAGTG 1 cut(s) 83
BveI ACCTGC 1 cut(s) 535
Cac8I GCNNGC 3 cut(s) 43, 623, 710
CaiI CAGNNNCTG 1 cut(s) 51
CciI TCATGA 1 cut(s) 541
Cfr13I GGNCC 1 cut(s) 48
CseI GACGC 1 cut(s) 205
Csp6I GTAC 6 cut(s) 179, 229, 267, 293, 514, 579
CviAII CATG 3 cut(s) 102, 542, 709
CviJI RGCY 2 cut(s) 50, 625
CviKI_1 RGCY 2 cut(s) 50, 625
CviQI GTAC 6 cut(s) 179, 229, 267, 293, 514, 579
DdeI CTNAG 5 cut(s) 319, 339, 369, 571, 603
DpnI GATC 2 cut(s) 14, 201
DpnII GATC 2 cut(s) 12, 199
Eco105I TACGTA 1 cut(s) 718
EcoNI CCTNNNNNAGG 1 cut(s) 456
EcoO109I RGGNCCY 1 cut(s) 48
EcoRII CCWGG 2 cut(s) 50, 284
FaeI CATG 3 cut(s) 105, 545, 712
FaiI YATR 8 cut(s) 103, 248, 393, 419, 437, 543, 710, 715
FaqI GGGAC 1 cut(s) 269
FatI CATG 3 cut(s) 101, 541, 708
Fnu4HI GCNGC 1 cut(s) 588
FokI GGATG 4 cut(s) 152, 245, 317, 674
Fsp4HI GCNGC 1 cut(s) 588
GluI GCNGC 1 cut(s) 588
GsaI CCCAGC 1 cut(s) 23
GsuI CTGGAG 2 cut(s) 73, 253
HaeIII GGCC 2 cut(s) 50, 625
HapII CCGG 2 cut(s) 558, 626
HgaI GACGC 1 cut(s) 205
Hin1II CATG 3 cut(s) 105, 545, 712
HinfI GANTC 1 cut(s) 205
HpaII CCGG 2 cut(s) 558, 626
HphI GGTGA 1 cut(s) 433
Hpy166II GTNNAC 1 cut(s) 83
Hpy188I TCNGA 4 cut(s) 204, 313, 340, 574
Hpy188III TCNNGA 5 cut(s) 116, 355, 459, 542, 652
Hpy8I GTNNAC 1 cut(s) 83
Hpy99I CGWCG 1 cut(s) 186
HpyAV CCTTC 5 cut(s) 24, 71, 246, 418, 489
HpyCH4III ACNGT 3 cut(s) 129, 518, 583
HpyCH4IV ACGT 3 cut(s) 181, 304, 717
HpyCH4V TGCA 3 cut(s) 225, 551, 621
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 222
HpyF3I CTNAG 5 cut(s) 319, 339, 369, 571, 603
HpySE526I ACGT 3 cut(s) 181, 304, 717
Hsp92II CATG 3 cut(s) 105, 545, 712
Kzo9I GATC 2 cut(s) 12, 199
LmnI GCTCC 2 cut(s) 277, 642
Lsp1109I GCAGC 1 cut(s) 599
LweI GCATC 1 cut(s) 654
MaeII ACGT 3 cut(s) 181, 304, 717
MalI GATC 2 cut(s) 14, 201
MboI GATC 2 cut(s) 12, 199
MboII GAAGA 9 cut(s) 7, 69, 72, 75, 78, 81, 530, 591, 640
MfeI CAATTG 1 cut(s) 240
MluCI AATT 5 cut(s) 35, 240, 533, 546, 609
MmeI TCCRAC 3 cut(s) 336, 620, 639
MnlI CCTC 7 cut(s) 18, 129, 197, 230, 334, 409, 580
MroXI GAANNNNTTC 1 cut(s) 673
MseI TTAA 1 cut(s) 212
MspCI CTTAAG 1 cut(s) 211
MspI CCGG 2 cut(s) 558, 626
MspR9I CCNGG 3 cut(s) 52, 286, 627
MunI CAATTG 1 cut(s) 240
MvaI CCWGG 2 cut(s) 52, 286
MwoI GCNNNNNNNGC 2 cut(s) 47, 222
NciI CCSGG 1 cut(s) 627
NdeII GATC 2 cut(s) 12, 199
NlaIII CATG 3 cut(s) 105, 545, 712
NspI RCATGY 1 cut(s) 712
PaeI GCATGC 1 cut(s) 712
PagI TCATGA 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 587
PdmI GAANNNNTTC 1 cut(s) 673
PfeI GAWTC 1 cut(s) 205
Pfl23II CGTACG 1 cut(s) 178
PflMI CCANNNNNTGG 1 cut(s) 408
PkrI GCNGC 1 cut(s) 589
Ppu21I YACGTR 1 cut(s) 718
Psp6I CCWGG 2 cut(s) 50, 284
PspFI CCCAGC 1 cut(s) 19
PspGI CCWGG 2 cut(s) 50, 284
PspLI CGTACG 1 cut(s) 178
PspPI GGNCC 1 cut(s) 48
PstNI CAGNNNCTG 1 cut(s) 51
RsaI GTAC 6 cut(s) 180, 230, 268, 294, 515, 580
RsaNI GTAC 6 cut(s) 179, 229, 267, 293, 514, 579
SaqAI TTAA 1 cut(s) 212
SatI GCNGC 1 cut(s) 588
Sau3AI GATC 2 cut(s) 12, 199
Sau96I GGNCC 1 cut(s) 48
ScaI AGTACT 3 cut(s) 230, 268, 294
ScrFI CCNGG 3 cut(s) 52, 286, 627
SetI ASST 8 cut(s) 82, 184, 307, 409, 529, 572, 676, 720
SfaNI GCATC 1 cut(s) 654
SfcI CTRYAG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 211, 484
SmoI CTYRAG 2 cut(s) 211, 484
SnaBI TACGTA 1 cut(s) 718
SphI GCATGC 1 cut(s) 712
Sse9I AATT 5 cut(s) 35, 240, 533, 546, 609
SsiI CCGC 2 cut(s) 262, 385
StyD4I CCNGG 3 cut(s) 50, 284, 625
TaaI ACNGT 3 cut(s) 129, 518, 583
TaiI ACGT 3 cut(s) 184, 307, 720
TaqI TCGA 3 cut(s) 11, 189, 653
TasI AATT 5 cut(s) 35, 240, 533, 546, 609
TatI WGTACW 3 cut(s) 228, 266, 292
TfiI GAWTC 1 cut(s) 205
Tru1I TTAA 1 cut(s) 212
Tru9I TTAA 1 cut(s) 212
TscAI CASTG 1 cut(s) 90
TseI GCWGC 1 cut(s) 587
TspDTI ATGAA 3 cut(s) 380, 530, 558
TspRI CASTG 1 cut(s) 90
Van91I CCANNNNNTGG 1 cut(s) 408
Vha464I CTTAAG 1 cut(s) 211
XagI CCTNNNNNAGG 1 cut(s) 456
XceI RCATGY 1 cut(s) 712
XcmI CCANNNNNNNNNTGG 1 cut(s) 282
XmnI GAANNNNTTC 1 cut(s) 673
ZrmI AGTACT 3 cut(s) 230, 268, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.