Rh6DG053300

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000144
Physical Location & Seq
Reverse (-)
5574908 .. 5576879
1972 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG053300.1

Sequence Viewer

Length: 696 bp
ATGTTGCGGCAAAGGAAGAATATGCTGCAGAAAAGGAAGAATACAGTGATGATTGAGAGAAAGGAAAAGCAAAATGTTGGTAGTGAGTGTAGCCCTTGGCAGACACCACCAGAAGATATCATGGAGATGATTCTACAGTTTGTCGACTTTGCTGATCAAAGGAGGTTAGGTCAGGTCAGCAAGTCTTGGTGGTCAATTATTTTCCAAACATTCATGCGTAGTGCTCGTCGTCAACTCCCATGGTTAATACTCCCTGAAGCCCAAAATTGCAGGACCAACAAGTACTTGAGCTTTCTCACCTTCCCTGAGAACAAACTCTCCAAGGGCAAAGCTGTCAAGTTAAAGCTGCCTAAGCAACTTAAAGGCGGGTGGATATACGGATCTTCTAAAGGTTGGTTGATCATCATCAATGAAAAAGGACTGAACTCTGAGATGTATCTAGTAAACCCAATTTCAAGAGCCGTACACAAACTTCCGCTCTTGAGAACAATTCCATCCTTCAAAAACTTTTTAAAAACCAGGAAATGGGAACTTTTGGGTGCCAATGGATTCTGTCTTCGCGTTAAAATGTCTACCTCGGATGGTTTTAATTCTTCAAGCAACTTTACTGTAGCTGCATATTTCGAGGATCAGAAGACTAGGTTTGTGCAGACCAGGAGACCAAAAATGGAGTGTCTTCCCGGTATTAGATGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

231

Amino Acids

27.11

Weight (kDa)

10.36

Isoelectric Point (pI)

59.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 106 - 214 1.2e-09 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 539
AccB7I CCANNNNNTGG 1 cut(s) 525
AccBSI CCGCTC 1 cut(s) 478
AccI GTMKAC 2 cut(s) 144, 572
AccII CGCG 1 cut(s) 561
AciI CCGC 3 cut(s) 7, 366, 476
AclWI GGATC 2 cut(s) 388, 636
AcuI CTGAAG 1 cut(s) 276
AfaI GTAC 2 cut(s) 284, 465
AfiI CCNNNNNNNGG 1 cut(s) 525
AgsI TTSAA 3 cut(s) 456, 502, 597
AjnI CCWGG 2 cut(s) 518, 653
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
AluBI AGCT 4 cut(s) 291, 332, 346, 614
AluI AGCT 4 cut(s) 291, 332, 346, 614
Alw21I GWGCWC 1 cut(s) 226
Alw26I GTCTC 1 cut(s) 652
AlwI GGATC 2 cut(s) 388, 636
ApeKI GCWGC 3 cut(s) 25, 346, 614
AspS9I GGNCC 1 cut(s) 273
AsuC2I CCSGG 1 cut(s) 681
AsuHPI GGTGA 1 cut(s) 289
AvaII GGWCC 1 cut(s) 273
BanI GGYRCC 1 cut(s) 539
BarI GAAGNNNNNNTAC 2 cut(s) 456, 488
BbsI GAAGAC 3 cut(s) 548, 641, 668
Bbv12I GWGCWC 1 cut(s) 226
BbvI GCAGC 3 cut(s) 12, 333, 601
BccI CCATC 3 cut(s) 502, 575, 684
BceAI ACGGC 1 cut(s) 446
BciT130I CCWGG 2 cut(s) 520, 655
BclI TGATCA 2 cut(s) 154, 399
BcnI CCSGG 1 cut(s) 681
BcoDI GTCTC 1 cut(s) 652
BfaI CTAG 2 cut(s) 440, 639
BfmI CTRYAG 3 cut(s) 26, 134, 609
BisI GCNGC 4 cut(s) 8, 26, 347, 615
BlsI GCNGC 4 cut(s) 9, 27, 348, 616
BmcAI AGTACT 1 cut(s) 284
Bme1390I CCNGG 3 cut(s) 520, 655, 681
Bme18I GGWCC 1 cut(s) 273
BmgT120I GGNCC 1 cut(s) 273
BmiI GGNNCC 1 cut(s) 541
BmrFI CCNGG 3 cut(s) 520, 655, 681
BpiI GAAGAC 3 cut(s) 548, 641, 668
Bpu10I CCTNAGC 1 cut(s) 351
BpuEI CTTGAG 2 cut(s) 307, 502
BpuMI CCSGG 1 cut(s) 681
BsaBI GATNNNNATC 1 cut(s) 404
BsaI GGTCTC 1 cut(s) 652
BsaJI CCNNGG 4 cut(s) 95, 239, 321, 576
BsaXI ACNNNNNCTCC 4 cut(s) 154, 184, 302, 332
Bsc4I CCNNNNNNNGG 1 cut(s) 525
Bse8I GATNNNNATC 1 cut(s) 404
BseBI CCWGG 2 cut(s) 520, 655
BseDI CCNNGG 4 cut(s) 95, 239, 321, 576
BseGI GGATG 2 cut(s) 494, 586
BseJI GATNNNNATC 1 cut(s) 404
BseLI CCNNNNNNNGG 1 cut(s) 525
BseMII CTCAG 2 cut(s) 297, 420
BseXI GCAGC 3 cut(s) 12, 333, 601
BsgI GTGCAG 1 cut(s) 668
Bsh1236I CGCG 1 cut(s) 561
BshNI GGYRCC 1 cut(s) 539
BsiHKAI GWGCWC 1 cut(s) 226
BsiSI CCGG 1 cut(s) 681
BslI CCNNNNNNNGG 1 cut(s) 525
BsmAI GTCTC 1 cut(s) 652
Bso31I GGTCTC 1 cut(s) 652
Bsp1286I GDGCHC 1 cut(s) 226
Bsp143I GATC 4 cut(s) 154, 380, 399, 628
Bsp19I CCATGG 1 cut(s) 239
BspACI CCGC 3 cut(s) 7, 366, 476
BspCNI CTCAG 2 cut(s) 298, 421
BspFNI CGCG 1 cut(s) 561
BspLI GGNNCC 1 cut(s) 541
BspMAI CTGCAG 1 cut(s) 30
BspPI GGATC 2 cut(s) 388, 636
BspT107I GGYRCC 1 cut(s) 539
BspTNI GGTCTC 1 cut(s) 652
BsrBI CCGCTC 1 cut(s) 478
BssECI CCNNGG 4 cut(s) 95, 239, 321, 576
BssMI GATC 4 cut(s) 154, 380, 399, 628
BssT1I CCWWGG 3 cut(s) 95, 239, 321
Bst2UI CCWGG 2 cut(s) 520, 655
Bst4CI ACNGT 3 cut(s) 46, 138, 610
BstDEI CTNAG 3 cut(s) 306, 351, 429
BstDSI CCRYGG 1 cut(s) 239
BstF5I GGATG 2 cut(s) 494, 586
BstFNI CGCG 1 cut(s) 561
BstKTI GATC 4 cut(s) 157, 383, 402, 631
BstMAI GTCTC 1 cut(s) 652
BstMBI GATC 4 cut(s) 154, 380, 399, 628
BstMWI GCNNNNNNNGC 1 cut(s) 352
BstNI CCWGG 2 cut(s) 520, 655
BstSCI CCNGG 3 cut(s) 518, 653, 679
BstSFI CTRYAG 3 cut(s) 26, 134, 609
BstUI CGCG 1 cut(s) 561
BstV1I GCAGC 3 cut(s) 12, 333, 601
BstV2I GAAGAC 3 cut(s) 548, 641, 668
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
BtgI CCRYGG 1 cut(s) 239
BtsCI GGATG 2 cut(s) 494, 586
BtsIMutI CAGTG 1 cut(s) 51
Cfr13I GGNCC 1 cut(s) 273
Csp6I GTAC 2 cut(s) 283, 464
CviAII CATG 3 cut(s) 121, 214, 240
CviJI RGCY 7 cut(s) 93, 260, 291, 332, 346, 461, 614
CviKI_1 RGCY 7 cut(s) 93, 260, 291, 332, 346, 461, 614
CviQI GTAC 2 cut(s) 283, 464
DdeI CTNAG 3 cut(s) 306, 351, 429
DpnI GATC 4 cut(s) 156, 382, 401, 630
DpnII GATC 4 cut(s) 154, 380, 399, 628
DraI TTTAAA 1 cut(s) 513
Eco130I CCWWGG 3 cut(s) 95, 239, 321
Eco31I GGTCTC 1 cut(s) 652
Eco32I GATATC 1 cut(s) 118
Eco47I GGWCC 1 cut(s) 273
Eco57I CTGAAG 1 cut(s) 276
EcoRII CCWGG 2 cut(s) 518, 653
EcoRV GATATC 1 cut(s) 118
EcoT14I CCWWGG 3 cut(s) 95, 239, 321
ErhI CCWWGG 3 cut(s) 95, 239, 321
FaeI CATG 3 cut(s) 124, 217, 243
FaiI YATR 6 cut(s) 23, 122, 215, 241, 376, 619
FatI CATG 3 cut(s) 120, 213, 239
FauI CCCGC 1 cut(s) 359
FbaI TGATCA 2 cut(s) 154, 399
FblI GTMKAC 2 cut(s) 144, 572
Fnu4HI GCNGC 4 cut(s) 8, 26, 347, 615
FokI GGATG 2 cut(s) 481, 593
Fsp4HI GCNGC 4 cut(s) 8, 26, 347, 615
FspBI CTAG 2 cut(s) 440, 639
GluI GCNGC 4 cut(s) 8, 26, 347, 615
HapII CCGG 1 cut(s) 681
Hin1II CATG 3 cut(s) 124, 217, 243
HincII GTYRAC 2 cut(s) 145, 233
HindII GTYRAC 2 cut(s) 145, 233
HinfI GANTC 2 cut(s) 130, 549
HpaII CCGG 1 cut(s) 681
HphI GGTGA 1 cut(s) 289
Hpy166II GTNNAC 5 cut(s) 145, 233, 445, 466, 573
Hpy188I TCNGA 3 cut(s) 430, 580, 633
Hpy188III TCNNGA 2 cut(s) 456, 481
Hpy8I GTNNAC 5 cut(s) 145, 233, 445, 466, 573
Hpy99I CGWCG 1 cut(s) 231
HpyAV CCTTC 2 cut(s) 310, 508
HpyCH4III ACNGT 3 cut(s) 46, 138, 610
HpyCH4V TGCA 4 cut(s) 28, 270, 617, 649
HpyF10VI GCNNNNNNNGC 1 cut(s) 352
HpyF3I CTNAG 3 cut(s) 306, 351, 429
Hsp92II CATG 3 cut(s) 124, 217, 243
Ksp22I TGATCA 2 cut(s) 154, 399
Kzo9I GATC 4 cut(s) 154, 380, 399, 628
LpnPI CCDG 9 cut(s) 123, 158, 256, 267, 318, 505, 532, 640, 667
Lsp1109I GCAGC 3 cut(s) 12, 333, 601
MaeI CTAG 2 cut(s) 440, 639
MalI GATC 4 cut(s) 156, 382, 401, 630
MbiI CCGCTC 1 cut(s) 478
MboI GATC 4 cut(s) 154, 380, 399, 628
MboII GAAGA 8 cut(s) 28, 49, 125, 375, 548, 585, 646, 668
MflI RGATCY 1 cut(s) 380
MhlI GDGCHC 1 cut(s) 226
MluCI AATT 5 cut(s) 195, 265, 450, 489, 589
MnlI CCTC 3 cut(s) 156, 586, 619
MseI TTAA 6 cut(s) 245, 341, 360, 512, 564, 588
MslI CAYNNNNRTG 1 cut(s) 125
MspI CCGG 1 cut(s) 681
MspR9I CCNGG 3 cut(s) 520, 655, 681
MvaI CCWGG 2 cut(s) 520, 655
MvnI CGCG 1 cut(s) 561
MwoI GCNNNNNNNGC 1 cut(s) 352
NciI CCSGG 1 cut(s) 681
NcoI CCATGG 1 cut(s) 239
NdeII GATC 4 cut(s) 154, 380, 399, 628
NlaIII CATG 3 cut(s) 124, 217, 243
NlaIV GGNNCC 1 cut(s) 541
PfeI GAWTC 2 cut(s) 130, 549
PflMI CCANNNNNTGG 1 cut(s) 525
PkrI GCNGC 4 cut(s) 9, 27, 348, 616
Psp6I CCWGG 2 cut(s) 518, 653
PspGI CCWGG 2 cut(s) 518, 653
PspN4I GGNNCC 1 cut(s) 541
PspPI GGNCC 1 cut(s) 273
PstI CTGCAG 1 cut(s) 30
PsuI RGATCY 1 cut(s) 380
RsaI GTAC 2 cut(s) 284, 465
RsaNI GTAC 2 cut(s) 283, 464
RseI CAYNNNNRTG 1 cut(s) 125
SalI GTCGAC 1 cut(s) 143
SaqAI TTAA 6 cut(s) 245, 341, 360, 512, 564, 588
SatI GCNGC 4 cut(s) 8, 26, 347, 615
Sau3AI GATC 4 cut(s) 154, 380, 399, 628
Sau96I GGNCC 1 cut(s) 273
ScaI AGTACT 1 cut(s) 284
ScrFI CCNGG 3 cut(s) 520, 655, 681
SduI GDGCHC 1 cut(s) 226
SfcI CTRYAG 3 cut(s) 26, 134, 609
SinI GGWCC 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 125
SmlI CTYRAG 2 cut(s) 286, 481
SmoI CTYRAG 2 cut(s) 286, 481
Sse9I AATT 5 cut(s) 195, 265, 450, 489, 589
SsiI CCGC 3 cut(s) 7, 366, 476
SspMI CTAG 2 cut(s) 440, 639
StyD4I CCNGG 3 cut(s) 518, 653, 679
StyI CCWWGG 3 cut(s) 95, 239, 321
TaaI ACNGT 3 cut(s) 46, 138, 610
TaqI TCGA 2 cut(s) 144, 624
TasI AATT 5 cut(s) 195, 265, 450, 489, 589
TatI WGTACW 1 cut(s) 282
TauI GCSGC 1 cut(s) 10
TfiI GAWTC 2 cut(s) 130, 549
Tru1I TTAA 6 cut(s) 245, 341, 360, 512, 564, 588
Tru9I TTAA 6 cut(s) 245, 341, 360, 512, 564, 588
TscAI CASTG 1 cut(s) 51
TseI GCWGC 3 cut(s) 25, 346, 614
TspDTI ATGAA 2 cut(s) 202, 426
TspGWI ACGGA 1 cut(s) 393
TspRI CASTG 1 cut(s) 51
Van91I CCANNNNNTGG 1 cut(s) 525
VpaK11BI GGWCC 1 cut(s) 273
XmiI GTMKAC 2 cut(s) 144, 572
XspI CTAG 2 cut(s) 440, 639
ZrmI AGTACT 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.