Rorug06G0132700

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000144
Physical Location & Seq
Forward (+)
18727823 .. 18732220
4398 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0132700.1

Sequence Viewer

Length: 1617 bp
ATGTCTGCACCTGATGCTGATGGACGTCCTGCTGAAAACTCTGAGGATGAGAAGAAAACAAAGCTTGGGTCTTTTAAGAAGGCAATTTCGAATTCCTTCAATAAGGGCCGCAGGAACAGTAAGGTAATGTCTTTCCCTATGGATGACATTGATGCAGAGGAGTTAAAGTCAGCCGAAGAATTTCGGCAAGCACTTATTGCGGAAGAACTGCTGCCTTCAAAACATGATGATACTCATATGCTGCTAAGATTTTTAAGGGCCAGGAAGTTTGACCCTGAGAAAACAAAGCAAATGTGGTCTGATATGCTCCACTGGAGGAAGGAATTTGGTTCTGACACTATTATGGAGGACTTCGAATTCAAGGAAATTGATGAAGTCTTGAAATATTATCCACAAGGGCATCATGGAGTTGATAAGGATGGACGCCCTGTCTATATTGAGAAACTTGGTCAAGTAGATTCCAACAAGCTGTTGCAAGTGACAACAATGGACAGATATGTGAAGTACCATGTACGAGAGTTTGAGAGGACGTTTGTGCATAAATTCCCTGCCTGTTCCATTGCGGCGAAGAAGCACATTGATCAGAGCACTACCATTTTGGATGTACAAGGAGTGGGGCTGACAAGTTTGAACAAATCTGCAAGGGAACTCATCCAACGCCTTCAGAAAGTTGACGGTGACAATTATCCTGAGACCCTGAACCGTATGTTCATCATCAATGCTGGTTCTGGATTTAGGCTCTTATGGAACACTGTCAAATCTTTCCTTGACCCCAAGACCACTGCAAAAATCAATGTACTGGGAAACCAATACCAAAGCAAGTTGCTTGAAATAATTGAAGCTAGCGAGCTGCCAGAGTTTTTGGGAGGTACTTGTACTTGTTCTGATAAGGGTGGCTGTATGCTTTCTGATAAGGGCCCATGGAATGATCTAGAAATAATGAAGATGGTTCAGAATTATGGTGAGGACCGTGAGGTTGACGCCAAATCCTCTAGCACAACTATATCCGGCAATGTGGATTCAAGCAATAAGGAGTTGGGCAAAAAGGAATCAGGCAAAAAGGAGTCAGGCAAAAAGGAGTCGGGCAAAAATGAGTCGGGTAAAAATGAGTCGGGCAAACATGAGTCGGACAAGAAGGAGTTGGACAAGAAGGAGGAGAAGAAGGTCGCTGAAGAAGAAAAAATCTCTGAAGATATGAAGGGTCCCCCAAATCGATCTGATTCAGTCTGCTCGGAGGCTTTTCTGGAGGCTAATGACATGCATATGGATCATCTCTCTCCTGTGCCTGAAGAACTGTCAACTCCCATGGGAGGTGAAAAAGAGGAAAAGCTTAGAGCTGCTCTTACTGCCTTGGAACAACAACTGTCTACAACCAAGCAGGCTGAGGTTGAGAAAGAGCAAAAGCTGAAGGCTGCTCTTGATCGCGTCAATGCCTTGGAACAAGAGCTAACAGAAACCAAGAAGGCACTTGATGAAGCCCGTGTTAAACAAGAGGAGCTTCTAGCCTCTGTTGGGAAGGAAAAGAAAGCCTATTCTGTTGGTGAGGCGCACCATATAAACATATATGTAGTATGGTGGATTAGTGGCTCAAAGTTTGAGTGGTTCTCATCAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

538

Amino Acids

60.87

Weight (kDa)

5.67

Isoelectric Point (pI)

44.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_N PF03765 60 - 100 1.2e-07 CRAL/TRIO, N-terminal domain
CRAL_TRIO PF00650 128 - 290 7.1e-30 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 28
AccI GTMKAC 1 cut(s) 1367
AccII CGCG 1 cut(s) 1425
AciI CCGC 3 cut(s) 109, 200, 563
AclWI GGATC 1 cut(s) 1275
AcsI RAATTY 5 cut(s) 91, 179, 323, 356, 542
AcuI CTGAAG 5 cut(s) 647, 1191, 1209, 1308, 1427
AcyI GRCGYC 3 cut(s) 25, 424, 981
AfaI GTAC 6 cut(s) 506, 513, 606, 798, 871, 877
AfiI CCNNNNNNNGG 2 cut(s) 1310, 1512
AgsI TTSAA 8 cut(s) 100, 219, 361, 382, 631, 830, 839, 1023
AhdI GACNNNNNGTC 1 cut(s) 428
AjnI CCWGG 1 cut(s) 260
AluBI AGCT 9 cut(s) 64, 469, 842, 850, 1330, 1337, 1405, 1447, 1498
AluI AGCT 9 cut(s) 64, 469, 842, 850, 1330, 1337, 1405, 1447, 1498
Alw21I GWGCWC 1 cut(s) 590
Alw26I GTCTC 1 cut(s) 686
AlwI GGATC 1 cut(s) 1275
AoxI GGCC 3 cut(s) 106, 258, 916
ApaI GGGCCC 1 cut(s) 920
ApeKI GCWGC 5 cut(s) 211, 241, 850, 1337, 1412
ApoI RAATTY 5 cut(s) 91, 179, 323, 356, 542
ArsI GACNNNNNNTTYG 2 cut(s) 53, 85
Asp700I GAANNNNTTC 2 cut(s) 95, 180
AspLEI GCGC 1 cut(s) 1549
AspS9I GGNCC 6 cut(s) 106, 258, 916, 917, 967, 1202
AsuHPI GGTGA 4 cut(s) 689, 974, 1325, 1553
AsuII TTCGAA 2 cut(s) 89, 354
AsuNHI GCTAGC 1 cut(s) 842
AvaII GGWCC 2 cut(s) 967, 1202
BaeGI GKGCMC 1 cut(s) 920
BanII GRGCYC 1 cut(s) 920
Bbv12I GWGCWC 1 cut(s) 590
BbvCI CCTCAGC 1 cut(s) 1383
BbvI GCAGC 5 cut(s) 198, 228, 837, 1324, 1399
BccI CCATC 3 cut(s) 14, 413, 940
BciT130I CCWGG 1 cut(s) 262
BclI TGATCA 1 cut(s) 580
BcoDI GTCTC 1 cut(s) 686
BfaI CTAG 4 cut(s) 843, 932, 993, 1502
BisI GCNGC 7 cut(s) 109, 212, 242, 564, 851, 1338, 1413
BlsI GCNGC 7 cut(s) 110, 213, 243, 565, 852, 1339, 1414
Bme1390I CCNGG 1 cut(s) 262
Bme18I GGWCC 2 cut(s) 967, 1202
BmeRI GACNNNNNGTC 1 cut(s) 428
BmgT120I GGNCC 6 cut(s) 106, 258, 916, 917, 967, 1202
BmiI GGNNCC 3 cut(s) 918, 1203, 1204
BmrFI CCNGG 1 cut(s) 262
BmrI ACTGGG 1 cut(s) 809
BmsI GCATC 3 cut(s) 4, 142, 409
BmtI GCTAGC 1 cut(s) 846
BmuI ACTGGG 1 cut(s) 809
BplI GAGNNNNNCTC 1 cut(s) 1589
BpmI CTGGAG 2 cut(s) 334, 1265
Bpu10I CCTNAGC 1 cut(s) 1383
Bpu14I TTCGAA 2 cut(s) 89, 354
Bsa29I ATCGAT 1 cut(s) 1213
BsaHI GRCGYC 3 cut(s) 25, 424, 981
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 4 cut(s) 920, 1305, 1350, 1434
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 2 cut(s) 1310, 1512
Bse1I ACTGG 2 cut(s) 317, 804
Bse3DI GCAATG 2 cut(s) 558, 1018
BseBI CCWGG 1 cut(s) 262
BseCI ATCGAT 1 cut(s) 1213
BseDI CCNNGG 4 cut(s) 920, 1305, 1350, 1434
BseGI GGATG 5 cut(s) 52, 148, 424, 607, 651
BseLI CCNNNNNNNGG 2 cut(s) 1310, 1512
BseMI GCAATG 2 cut(s) 558, 1018
BseMII CTCAG 4 cut(s) 33, 267, 681, 1374
BseNI ACTGG 2 cut(s) 317, 804
BseRI GAGGAG 3 cut(s) 173, 1169, 1508
BseSI GKGCMC 1 cut(s) 920
BseXI GCAGC 5 cut(s) 198, 228, 837, 1324, 1399
Bsh1236I CGCG 1 cut(s) 1425
BshFI GGCC 3 cut(s) 108, 260, 918
BshVI ATCGAT 1 cut(s) 1213
BsiHKAI GWGCWC 1 cut(s) 590
BsiSI CCGG 1 cut(s) 1008
BslFI GGGAC 1 cut(s) 1188
BslI CCNNNNNNNGG 2 cut(s) 1310, 1512
BsmAI GTCTC 1 cut(s) 686
BsmFI GGGAC 1 cut(s) 1188
BsnI GGCC 3 cut(s) 108, 260, 918
Bso31I GGTCTC 1 cut(s) 686
Bsp119I TTCGAA 2 cut(s) 89, 354
Bsp120I GGGCCC 1 cut(s) 916
Bsp1286I GDGCHC 2 cut(s) 590, 920
Bsp1407I TGTACA 1 cut(s) 604
Bsp143I GATC 5 cut(s) 580, 928, 1214, 1267, 1420
Bsp19I CCATGG 2 cut(s) 920, 1305
BspACI CCGC 3 cut(s) 109, 200, 563
BspANI GGCC 3 cut(s) 108, 260, 918
BspCNI CTCAG 4 cut(s) 34, 268, 682, 1375
BspDI ATCGAT 1 cut(s) 1213
BspFNI CGCG 1 cut(s) 1425
BspLI GGNNCC 3 cut(s) 918, 1203, 1204
BspOI GCTAGC 1 cut(s) 846
BspPI GGATC 1 cut(s) 1275
BspT104I TTCGAA 2 cut(s) 89, 354
BspTNI GGTCTC 1 cut(s) 686
BsrDI GCAATG 2 cut(s) 558, 1018
BsrGI TGTACA 1 cut(s) 604
BsrI ACTGG 2 cut(s) 317, 804
BssECI CCNNGG 4 cut(s) 920, 1305, 1350, 1434
BssMI GATC 5 cut(s) 580, 928, 1214, 1267, 1420
BssNI GRCGYC 3 cut(s) 25, 424, 981
BssT1I CCWWGG 4 cut(s) 920, 1305, 1350, 1434
Bst2UI CCWGG 1 cut(s) 262
Bst4CI ACNGT 7 cut(s) 119, 677, 704, 754, 971, 1296, 1365
BstACI GRCGYC 3 cut(s) 25, 424, 981
BstAPI GCANNNNNTGC 2 cut(s) 14, 197
BstAUI TGTACA 1 cut(s) 604
BstBI TTCGAA 2 cut(s) 89, 354
BstC8I GCNNGC 4 cut(s) 189, 844, 848, 1380
BstDEI CTNAG 6 cut(s) 42, 245, 276, 690, 1331, 1383
BstDSI CCRYGG 2 cut(s) 920, 1305
BstF5I GGATG 5 cut(s) 52, 148, 424, 607, 651
BstFNI CGCG 1 cut(s) 1425
BstHHI GCGC 1 cut(s) 1549
BstKTI GATC 5 cut(s) 583, 931, 1217, 1270, 1423
BstMAI GTCTC 1 cut(s) 686
BstMBI GATC 5 cut(s) 580, 928, 1214, 1267, 1420
BstMWI GCNNNNNNNGC 3 cut(s) 14, 197, 1346
BstNI CCWGG 1 cut(s) 262
BstNSI RCATGY 1 cut(s) 1261
BstSCI CCNGG 1 cut(s) 260
BstSLI GKGCMC 1 cut(s) 920
BstUI CGCG 1 cut(s) 1425
BstV1I GCAGC 5 cut(s) 198, 228, 837, 1324, 1399
Bsu15I ATCGAT 1 cut(s) 1213
BsuRI GGCC 3 cut(s) 108, 260, 918
BsuTUI ATCGAT 1 cut(s) 1213
BtgI CCRYGG 2 cut(s) 920, 1305
BtsCI GGATG 5 cut(s) 52, 148, 424, 607, 651
BtsI GCAGTG 1 cut(s) 780
BtsIMutI CAGTG 3 cut(s) 310, 750, 780
Cac8I GCNNGC 4 cut(s) 189, 844, 848, 1380
CfoI GCGC 1 cut(s) 1549
Cfr13I GGNCC 6 cut(s) 106, 258, 916, 917, 967, 1202
ClaI ATCGAT 1 cut(s) 1213
CseI GACGC 3 cut(s) 432, 989, 1414
Csp6I GTAC 6 cut(s) 505, 512, 605, 797, 870, 876
CviAII CATG 7 cut(s) 224, 404, 509, 921, 1121, 1258, 1306
CviQI GTAC 6 cut(s) 505, 512, 605, 797, 870, 876
DdeI CTNAG 6 cut(s) 42, 245, 276, 690, 1331, 1383
DpnI GATC 5 cut(s) 582, 930, 1216, 1269, 1422
DpnII GATC 5 cut(s) 580, 928, 1214, 1267, 1420
DriI GACNNNNNGTC 1 cut(s) 428
Eam1105I GACNNNNNGTC 1 cut(s) 428
Eco130I CCWWGG 4 cut(s) 920, 1305, 1350, 1434
Eco24I GRGCYC 1 cut(s) 920
Eco31I GGTCTC 1 cut(s) 686
Eco47I GGWCC 2 cut(s) 967, 1202
Eco57I CTGAAG 5 cut(s) 647, 1191, 1209, 1308, 1427
EcoO109I RGGNCCY 2 cut(s) 916, 1202
EcoRI GAATTC 2 cut(s) 91, 356
EcoRII CCWGG 1 cut(s) 260
EcoT14I CCWWGG 4 cut(s) 920, 1305, 1350, 1434
EcoT22I ATGCAT 1 cut(s) 1263
EcoT38I GRGCYC 1 cut(s) 920
ErhI CCWWGG 4 cut(s) 920, 1305, 1350, 1434
FaeI CATG 7 cut(s) 227, 407, 512, 924, 1124, 1261, 1309
FalI AAGNNNNNCTT 2 cut(s) 1482, 1514
FaqI GGGAC 1 cut(s) 1188
FatI CATG 7 cut(s) 223, 403, 508, 920, 1120, 1257, 1305
FauNDI CATATG 2 cut(s) 237, 1263
FbaI TGATCA 1 cut(s) 580
FblI GTMKAC 1 cut(s) 1367
Fnu4HI GCNGC 7 cut(s) 109, 212, 242, 564, 851, 1338, 1413
FokI GGATG 5 cut(s) 59, 155, 431, 614, 638
FriOI GRGCYC 1 cut(s) 920
Fsp4HI GCNGC 7 cut(s) 109, 212, 242, 564, 851, 1338, 1413
FspBI CTAG 4 cut(s) 843, 932, 993, 1502
GlaI GCGC 1 cut(s) 1548
GluI GCNGC 7 cut(s) 109, 212, 242, 564, 851, 1338, 1413
GsuI CTGGAG 2 cut(s) 334, 1265
HaeIII GGCC 3 cut(s) 108, 260, 918
HapII CCGG 1 cut(s) 1008
HgaI GACGC 3 cut(s) 432, 989, 1414
HhaI GCGC 1 cut(s) 1549
Hin1I GRCGYC 3 cut(s) 25, 424, 981
Hin1II CATG 7 cut(s) 227, 407, 512, 924, 1124, 1261, 1309
Hin6I GCGC 1 cut(s) 1547
HinP1I GCGC 1 cut(s) 1547
HincII GTYRAC 3 cut(s) 673, 979, 1299
HindII GTYRAC 3 cut(s) 673, 979, 1299
HindIII AAGCTT 2 cut(s) 62, 1328
HinfI GANTC 9 cut(s) 458, 1019, 1049, 1064, 1079, 1094, 1109, 1124, 1220
HpaII CCGG 1 cut(s) 1008
HphI GGTGA 4 cut(s) 689, 974, 1325, 1553
Hpy166II GTNNAC 4 cut(s) 673, 979, 1299, 1368
Hpy188III TCNNGA 7 cut(s) 379, 689, 729, 932, 1244, 1418, 1611
Hpy8I GTNNAC 4 cut(s) 673, 979, 1299, 1368
HpyCH4III ACNGT 7 cut(s) 119, 677, 704, 754, 971, 1296, 1365
HpyCH4IV ACGT 2 cut(s) 25, 530
HpyCH4V TGCA 7 cut(s) 8, 155, 475, 538, 641, 785, 1261
HpyF10VI GCNNNNNNNGC 3 cut(s) 14, 197, 1346
HpyF3I CTNAG 6 cut(s) 42, 245, 276, 690, 1331, 1383
HpySE526I ACGT 2 cut(s) 25, 530
Hsp92I GRCGYC 3 cut(s) 25, 424, 981
Hsp92II CATG 7 cut(s) 227, 407, 512, 924, 1124, 1261, 1309
HspAI GCGC 1 cut(s) 1547
KflI GGGWCCC 1 cut(s) 1202
Ksp22I TGATCA 1 cut(s) 580
Kzo9I GATC 5 cut(s) 580, 928, 1214, 1267, 1420
LmnI GCTCC 2 cut(s) 312, 1495
Lsp1109I GCAGC 5 cut(s) 198, 228, 837, 1324, 1399
LweI GCATC 3 cut(s) 4, 142, 409
MaeI CTAG 4 cut(s) 843, 932, 993, 1502
MaeII ACGT 2 cut(s) 25, 530
MaeIII GTNAC 2 cut(s) 478, 677
MalI GATC 5 cut(s) 582, 930, 1216, 1269, 1422
MboI GATC 5 cut(s) 580, 928, 1214, 1267, 1420
MhlI GDGCHC 2 cut(s) 590, 920
MlyI GAGTC 5 cut(s) 1073, 1088, 1103, 1118, 1133
MmeI TCCRAC 4 cut(s) 486, 679, 1107, 1122
Mph1103I ATGCAT 1 cut(s) 1263
MroXI GAANNNNTTC 2 cut(s) 95, 180
MseI TTAA 4 cut(s) 75, 164, 254, 1485
MslI CAYNNNNRTG 3 cut(s) 341, 1262, 1612
MspI CCGG 1 cut(s) 1008
MspR9I CCNGG 1 cut(s) 262
MvaI CCWGG 1 cut(s) 262
MvnI CGCG 1 cut(s) 1425
MwoI GCNNNNNNNGC 3 cut(s) 14, 197, 1346
NcoI CCATGG 2 cut(s) 920, 1305
NdeI CATATG 2 cut(s) 237, 1263
NdeII GATC 5 cut(s) 580, 928, 1214, 1267, 1420
NheI GCTAGC 1 cut(s) 842
NlaIII CATG 7 cut(s) 227, 407, 512, 924, 1124, 1261, 1309
NlaIV GGNNCC 3 cut(s) 918, 1203, 1204
NmuCI GTSAC 2 cut(s) 478, 677
NsiI ATGCAT 1 cut(s) 1263
NspI RCATGY 1 cut(s) 1261
NspV TTCGAA 2 cut(s) 89, 354
PdmI GAANNNNTTC 2 cut(s) 95, 180
PfeI GAWTC 4 cut(s) 458, 1019, 1049, 1220
PkrI GCNGC 7 cut(s) 110, 213, 243, 565, 852, 1339, 1414
PleI GAGTC 5 cut(s) 1072, 1087, 1102, 1117, 1132
PpsI GAGTC 5 cut(s) 1072, 1087, 1102, 1117, 1132
PpuMI RGGWCCY 1 cut(s) 1202
Psp5II RGGWCCY 1 cut(s) 1202
Psp6I CCWGG 1 cut(s) 260
PspGI CCWGG 1 cut(s) 260
PspN4I GGNNCC 3 cut(s) 918, 1203, 1204
PspOMI GGGCCC 1 cut(s) 916
PspPI GGNCC 6 cut(s) 106, 258, 916, 917, 967, 1202
PspPPI RGGWCCY 1 cut(s) 1202
RsaI GTAC 6 cut(s) 506, 513, 606, 798, 871, 877
RsaNI GTAC 6 cut(s) 505, 512, 605, 797, 870, 876
RseI CAYNNNNRTG 3 cut(s) 341, 1262, 1612
SaqAI TTAA 4 cut(s) 75, 164, 254, 1485
SatI GCNGC 7 cut(s) 109, 212, 242, 564, 851, 1338, 1413
Sau3AI GATC 5 cut(s) 580, 928, 1214, 1267, 1420
Sau96I GGNCC 6 cut(s) 106, 258, 916, 917, 967, 1202
SchI GAGTC 5 cut(s) 1073, 1088, 1103, 1118, 1133
ScrFI CCNGG 1 cut(s) 262
SduI GDGCHC 2 cut(s) 590, 920
SfaNI GCATC 3 cut(s) 4, 142, 409
SfuI TTCGAA 2 cut(s) 89, 354
SinI GGWCC 2 cut(s) 967, 1202
SmiMI CAYNNNNRTG 3 cut(s) 341, 1262, 1612
SsiI CCGC 3 cut(s) 109, 200, 563
SspI AATATT 1 cut(s) 386
SspMI CTAG 4 cut(s) 843, 932, 993, 1502
StyD4I CCNGG 1 cut(s) 260
StyI CCWWGG 4 cut(s) 920, 1305, 1350, 1434
TaaI ACNGT 7 cut(s) 119, 677, 704, 754, 971, 1296, 1365
TaiI ACGT 2 cut(s) 28, 533
TaqI TCGA 3 cut(s) 89, 354, 1213
TatI WGTACW 3 cut(s) 604, 796, 875
TauI GCSGC 2 cut(s) 111, 566
TfiI GAWTC 4 cut(s) 458, 1019, 1049, 1220
Tru1I TTAA 4 cut(s) 75, 164, 254, 1485
Tru9I TTAA 4 cut(s) 75, 164, 254, 1485
TscAI CASTG 3 cut(s) 317, 757, 787
TseFI GTSAC 2 cut(s) 478, 677
TseI GCWGC 5 cut(s) 211, 241, 850, 1337, 1412
Tsp45I GTSAC 2 cut(s) 478, 677
TspDTI ATGAA 5 cut(s) 387, 700, 956, 1211, 1488
TspRI CASTG 3 cut(s) 317, 757, 787
VpaK11BI GGWCC 2 cut(s) 967, 1202
XapI RAATTY 5 cut(s) 91, 179, 323, 356, 542
XbaI TCTAGA 1 cut(s) 931
XceI RCATGY 1 cut(s) 1261
XmiI GTMKAC 1 cut(s) 1367
XmnI GAANNNNTTC 2 cut(s) 95, 180
XspI CTAG 4 cut(s) 843, 932, 993, 1502
ZraI GACGTC 1 cut(s) 26
Zsp2I ATGCAT 1 cut(s) 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.