Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 1 cut(s) | 294 |
| AclWI | GGATC | 4 cut(s) | 37, 263, 299, 354 |
| AcsI | RAATTY | 3 cut(s) | 33, 51, 86 |
| AgsI | TTSAA | 1 cut(s) | 406 |
| AluBI | AGCT | 1 cut(s) | 395 |
| AluI | AGCT | 1 cut(s) | 395 |
| Alw26I | GTCTC | 1 cut(s) | 269 |
| AlwI | GGATC | 4 cut(s) | 37, 263, 299, 354 |
| AoxI | GGCC | 2 cut(s) | 97, 118 |
| ApoI | RAATTY | 3 cut(s) | 33, 51, 86 |
| AspA2I | CCTAGG | 1 cut(s) | 340 |
| AvrII | CCTAGG | 1 cut(s) | 340 |
| BccI | CCATC | 2 cut(s) | 54, 365 |
| BcoDI | GTCTC | 1 cut(s) | 269 |
| BfaI | CTAG | 3 cut(s) | 341, 392, 396 |
| BlnI | CCTAGG | 1 cut(s) | 340 |
| BmsI | GCATC | 1 cut(s) | 435 |
| BpuEI | CTTGAG | 1 cut(s) | 209 |
| BsaJI | CCNNGG | 1 cut(s) | 340 |
| BseDI | CCNNGG | 1 cut(s) | 340 |
| BseGI | GGATG | 3 cut(s) | 70, 151, 376 |
| BseMII | CTCAG | 1 cut(s) | 226 |
| BseRI | GAGGAG | 1 cut(s) | 185 |
| BshFI | GGCC | 2 cut(s) | 99, 120 |
| BslFI | GGGAC | 1 cut(s) | 370 |
| BsmAI | GTCTC | 1 cut(s) | 269 |
| BsmFI | GGGAC | 1 cut(s) | 370 |
| BsmI | GAATGC | 1 cut(s) | 183 |
| BsnI | GGCC | 2 cut(s) | 99, 120 |
| Bsp143I | GATC | 4 cut(s) | 42, 268, 304, 346 |
| BspANI | GGCC | 2 cut(s) | 99, 120 |
| BspCNI | CTCAG | 1 cut(s) | 225 |
| BspPI | GGATC | 4 cut(s) | 37, 263, 299, 354 |
| BssECI | CCNNGG | 1 cut(s) | 340 |
| BssMI | GATC | 4 cut(s) | 42, 268, 304, 346 |
| BssNAI | GTATAC | 1 cut(s) | 295 |
| BssT1I | CCWWGG | 1 cut(s) | 340 |
| Bst1107I | GTATAC | 1 cut(s) | 295 |
| BstDEI | CTNAG | 1 cut(s) | 212 |
| BstF5I | GGATG | 3 cut(s) | 70, 151, 376 |
| BstKTI | GATC | 4 cut(s) | 45, 271, 307, 349 |
| BstMAI | GTCTC | 1 cut(s) | 269 |
| BstMBI | GATC | 4 cut(s) | 42, 268, 304, 346 |
| BstX2I | RGATCY | 1 cut(s) | 268 |
| BstYI | RGATCY | 1 cut(s) | 268 |
| BstZ17I | GTATAC | 1 cut(s) | 295 |
| BsuRI | GGCC | 2 cut(s) | 99, 120 |
| BtsCI | GGATG | 3 cut(s) | 70, 151, 376 |
| BtsIMutI | CAGTG | 1 cut(s) | 220 |
| CviAII | CATG | 4 cut(s) | 101, 157, 176, 444 |
| CviJI | RGCY | 4 cut(s) | 99, 120, 197, 395 |
| CviKI_1 | RGCY | 4 cut(s) | 99, 120, 197, 395 |
| DdeI | CTNAG | 1 cut(s) | 212 |
| DpnI | GATC | 4 cut(s) | 44, 270, 306, 348 |
| DpnII | GATC | 4 cut(s) | 42, 268, 304, 346 |
| Eco130I | CCWWGG | 1 cut(s) | 340 |
| EcoT14I | CCWWGG | 1 cut(s) | 340 |
| ErhI | CCWWGG | 1 cut(s) | 340 |
| FaeI | CATG | 4 cut(s) | 104, 160, 179, 447 |
| FaqI | GGGAC | 1 cut(s) | 370 |
| FatI | CATG | 4 cut(s) | 100, 156, 175, 443 |
| FblI | GTMKAC | 1 cut(s) | 294 |
| FokI | GGATG | 3 cut(s) | 77, 158, 383 |
| FspBI | CTAG | 3 cut(s) | 341, 392, 396 |
| HaeIII | GGCC | 2 cut(s) | 99, 120 |
| Hin1II | CATG | 4 cut(s) | 104, 160, 179, 447 |
| HinfI | GANTC | 1 cut(s) | 17 |
| Hpy166II | GTNNAC | 1 cut(s) | 295 |
| Hpy188III | TCNNGA | 3 cut(s) | 188, 272, 308 |
| Hpy8I | GTNNAC | 1 cut(s) | 295 |
| HpyAV | CCTTC | 5 cut(s) | 54, 89, 110, 134, 398 |
| HpyCH4V | TGCA | 2 cut(s) | 426, 437 |
| HpyF3I | CTNAG | 1 cut(s) | 212 |
| Hsp92II | CATG | 4 cut(s) | 104, 160, 179, 447 |
| Kzo9I | GATC | 4 cut(s) | 42, 268, 304, 346 |
| LmnI | GCTCC | 1 cut(s) | 172 |
| LpnPI | CCDG | 4 cut(s) | 183, 258, 285, 321 |
| LweI | GCATC | 1 cut(s) | 435 |
| MaeI | CTAG | 3 cut(s) | 341, 392, 396 |
| MaeIII | GTNAC | 1 cut(s) | 411 |
| MalI | GATC | 4 cut(s) | 44, 270, 306, 348 |
| MboI | GATC | 4 cut(s) | 42, 268, 304, 346 |
| MboII | GAAGA | 7 cut(s) | 86, 143, 146, 149, 173, 278, 369 |
| MflI | RGATCY | 1 cut(s) | 268 |
| MluCI | AATT | 4 cut(s) | 33, 51, 86, 421 |
| MnlI | CCTC | 5 cut(s) | 76, 97, 106, 163, 361 |
| MseI | TTAA | 1 cut(s) | 252 |
| Mva1269I | GAATGC | 1 cut(s) | 183 |
| NdeII | GATC | 4 cut(s) | 42, 268, 304, 346 |
| NlaIII | CATG | 4 cut(s) | 104, 160, 179, 447 |
| PctI | GAATGC | 1 cut(s) | 183 |
| PfeI | GAWTC | 1 cut(s) | 17 |
| PsuI | RGATCY | 1 cut(s) | 268 |
| SaqAI | TTAA | 1 cut(s) | 252 |
| Sau3AI | GATC | 4 cut(s) | 42, 268, 304, 346 |
| SetI | ASST | 3 cut(s) | 333, 342, 397 |
| SfaNI | GCATC | 1 cut(s) | 435 |
| SmlI | CTYRAG | 1 cut(s) | 188 |
| SmoI | CTYRAG | 1 cut(s) | 188 |
| Sse9I | AATT | 4 cut(s) | 33, 51, 86, 421 |
| SspI | AATATT | 2 cut(s) | 256, 354 |
| SspMI | CTAG | 3 cut(s) | 341, 392, 396 |
| StyI | CCWWGG | 1 cut(s) | 340 |
| TasI | AATT | 4 cut(s) | 33, 51, 86, 421 |
| TfiI | GAWTC | 1 cut(s) | 17 |
| Tru1I | TTAA | 1 cut(s) | 252 |
| Tru9I | TTAA | 1 cut(s) | 252 |
| TscAI | CASTG | 1 cut(s) | 220 |
| TspDTI | ATGAA | 1 cut(s) | 87 |
| TspRI | CASTG | 1 cut(s) | 220 |
| XapI | RAATTY | 3 cut(s) | 33, 51, 86 |
| XmaJI | CCTAGG | 1 cut(s) | 340 |
| XmiI | GTMKAC | 1 cut(s) | 294 |
| XspI | CTAG | 3 cut(s) | 341, 392, 396 |