Rh6AG245400

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6A
OG0000144
Physical Location & Seq
Forward (+)
43402370 .. 43403161
792 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6AG245400.1

Sequence Viewer

Length: 792 bp
ATGAAAATCTTGTCGAGAGAGTCAACAGAATATTTTTTTTCCAGCACTGGCGTGCTATATGCCTTGGTTACAGGTAACCGCAATGGTCGTGGTGTTGATGCAGACCGCACTTTAAAGTTCGGACATGAGGCAGTGAAATTGAAGCTGATCTATGACATAGAGGAGTGGTGGATTGATGCCGGGGTTGATCCGGTAGGGCTTTTGTGCATGTCATACTTGTTGGAATCAACAAACAACGAAATTTTATTGATCCATCAAATTTTGAGAATGGATGTTGATGAAGATGATGAGGAATTTATGGAAGGCCATGAGGACAACGAGGAAGGCAATAACAACGAAGAAGAAGAAGGGGATGATAATGACATGGCAGATAATGAGGAGCATGAGAGCATTCTTGAGTGGGCTGGACAAACCTTACTCAGTGTTTATGCTAAAGCAAAGCAACTAAACCCAGATTTTAATATTGACAAGGAAGATCCAGAGACAATATGTTTTAGAGTATACAAGATTGATCCAGACAATAACTACTATCATAAGGTAGAAAACCTAGGGGATCAAATATTTTTTGTGGCAGAGGATGGTTCTTCCTTGTCCCTTCTAGCTAGTGATTTCAAAGAGTTACAAGGGAATTGCATCTATTTTGCAACAACAGGAAGTAATATAGAAACTTCCTGCATCTCTTGTGAGGTCGGCATTTTCTACCTGGATAGTGGAAAAATTGAGCAACCCTTTCCAATTGTTAAGATGCCAAGGTACTCTCATCTTAGTTGGTTCACCCCGAGTTTATGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

263

Amino Acids

30.09

Weight (kDa)

4.23

Isoelectric Point (pI)

47.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 111 - 215 2.9e-15 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 501
AciI CCGC 2 cut(s) 79, 106
AclWI GGATC 5 cut(s) 182, 244, 470, 506, 561
AcsI RAATTY 3 cut(s) 240, 258, 293
AfaI GTAC 1 cut(s) 755
AgsI TTSAA 2 cut(s) 142, 613
AjnI CCWGG 1 cut(s) 702
AleI CACNNNNGTG 1 cut(s) 50
AluBI AGCT 2 cut(s) 145, 602
AluI AGCT 2 cut(s) 145, 602
Alw26I GTCTC 1 cut(s) 476
AlwI GGATC 5 cut(s) 182, 244, 470, 506, 561
Ama87I CYCGRG 1 cut(s) 778
AoxI GGCC 1 cut(s) 304
ApoI RAATTY 3 cut(s) 240, 258, 293
AspA2I CCTAGG 1 cut(s) 547
AsuC2I CCSGG 1 cut(s) 181
AsuHPI GGTGA 1 cut(s) 766
AvaI CYCGRG 1 cut(s) 778
AvrII CCTAGG 1 cut(s) 547
BccI CCATC 2 cut(s) 261, 572
BciT130I CCWGG 1 cut(s) 704
BcnI CCSGG 1 cut(s) 181
BcoDI GTCTC 1 cut(s) 476
BfaI CTAG 3 cut(s) 548, 599, 603
BlnI CCTAGG 1 cut(s) 547
Bme1390I CCNGG 2 cut(s) 181, 704
BmeT110I CYCGRG 1 cut(s) 778
BmrFI CCNGG 2 cut(s) 181, 704
BmsI GCATC 5 cut(s) 88, 166, 642, 684, 735
BpuEI CTTGAG 1 cut(s) 416
BpuMI CCSGG 1 cut(s) 181
BsaJI CCNNGG 4 cut(s) 63, 180, 547, 749
BsaWI WCCGGW 1 cut(s) 190
Bse1I ACTGG 1 cut(s) 52
Bse3DI GCAATG 1 cut(s) 88
BseBI CCWGG 1 cut(s) 704
BseDI CCNNGG 4 cut(s) 63, 180, 547, 749
BseGI GGATG 3 cut(s) 277, 358, 583
BseMI GCAATG 1 cut(s) 88
BseMII CTCAG 1 cut(s) 433
BseNI ACTGG 1 cut(s) 52
BseRI GAGGAG 2 cut(s) 176, 392
BshFI GGCC 1 cut(s) 306
BsiHKCI CYCGRG 1 cut(s) 778
BsiSI CCGG 2 cut(s) 180, 191
BslFI GGGAC 1 cut(s) 577
BsmAI GTCTC 1 cut(s) 476
BsmFI GGGAC 1 cut(s) 577
BsmI GAATGC 1 cut(s) 390
BsnI GGCC 1 cut(s) 306
BsoBI CYCGRG 1 cut(s) 778
Bsp143I GATC 6 cut(s) 147, 187, 249, 475, 511, 553
BspACI CCGC 2 cut(s) 79, 106
BspANI GGCC 1 cut(s) 306
BspCNI CTCAG 1 cut(s) 432
BspPI GGATC 5 cut(s) 182, 244, 470, 506, 561
BsrDI GCAATG 1 cut(s) 88
BsrI ACTGG 1 cut(s) 52
BssECI CCNNGG 4 cut(s) 63, 180, 547, 749
BssMI GATC 6 cut(s) 147, 187, 249, 475, 511, 553
BssNAI GTATAC 1 cut(s) 502
BssT1I CCWWGG 3 cut(s) 63, 547, 749
Bst1107I GTATAC 1 cut(s) 502
Bst2UI CCWGG 1 cut(s) 704
BstC8I GCNNGC 1 cut(s) 53
BstDEI CTNAG 2 cut(s) 419, 764
BstEII GGTNACC 1 cut(s) 74
BstF5I GGATG 3 cut(s) 277, 358, 583
BstKTI GATC 6 cut(s) 150, 190, 252, 478, 514, 556
BstMAI GTCTC 1 cut(s) 476
BstMBI GATC 6 cut(s) 147, 187, 249, 475, 511, 553
BstNI CCWGG 1 cut(s) 704
BstNSI RCATGY 1 cut(s) 211
BstPI GGTNACC 1 cut(s) 74
BstSCI CCNGG 2 cut(s) 179, 702
BstX2I RGATCY 1 cut(s) 475
BstYI RGATCY 1 cut(s) 475
BstZ17I GTATAC 1 cut(s) 502
BsuRI GGCC 1 cut(s) 306
BtsCI GGATG 3 cut(s) 277, 358, 583
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 3 cut(s) 45, 138, 427
Cac8I GCNNGC 1 cut(s) 53
Csp6I GTAC 1 cut(s) 754
CspCI CAANNNNNGTGG 2 cut(s) 70, 105
CviAII CATG 5 cut(s) 125, 208, 308, 364, 383
CviJI RGCY 5 cut(s) 145, 199, 306, 404, 602
CviKI_1 RGCY 5 cut(s) 145, 199, 306, 404, 602
CviQI GTAC 1 cut(s) 754
DdeI CTNAG 2 cut(s) 419, 764
DpnI GATC 6 cut(s) 149, 189, 251, 477, 513, 555
DpnII GATC 6 cut(s) 147, 187, 249, 475, 511, 553
DraI TTTAAA 1 cut(s) 114
Eco130I CCWWGG 3 cut(s) 63, 547, 749
Eco88I CYCGRG 1 cut(s) 778
Eco91I GGTNACC 1 cut(s) 74
EcoO65I GGTNACC 1 cut(s) 74
EcoRII CCWGG 1 cut(s) 702
EcoT14I CCWWGG 3 cut(s) 63, 547, 749
ErhI CCWWGG 3 cut(s) 63, 547, 749
FaeI CATG 5 cut(s) 128, 211, 311, 367, 386
FaqI GGGAC 1 cut(s) 577
FatI CATG 5 cut(s) 124, 207, 307, 363, 382
FblI GTMKAC 1 cut(s) 501
FokI GGATG 3 cut(s) 284, 365, 590
FspBI CTAG 3 cut(s) 548, 599, 603
HaeIII GGCC 1 cut(s) 306
HapII CCGG 2 cut(s) 180, 191
Hin1II CATG 5 cut(s) 128, 211, 311, 367, 386
HincII GTYRAC 1 cut(s) 24
HindII GTYRAC 1 cut(s) 24
HinfI GANTC 2 cut(s) 20, 224
HpaII CCGG 2 cut(s) 180, 191
HphI GGTGA 1 cut(s) 766
Hpy166II GTNNAC 3 cut(s) 24, 502, 774
Hpy188I TCNGA 1 cut(s) 122
Hpy188III TCNNGA 4 cut(s) 15, 395, 479, 515
Hpy8I GTNNAC 3 cut(s) 24, 502, 774
HpyAV CCTTC 4 cut(s) 296, 317, 341, 605
HpyCH4V TGCA 5 cut(s) 101, 207, 633, 644, 675
HpyF3I CTNAG 2 cut(s) 419, 764
Hsp92II CATG 5 cut(s) 128, 211, 311, 367, 386
Kzo9I GATC 6 cut(s) 147, 187, 249, 475, 511, 553
LmnI GCTCC 1 cut(s) 379
LweI GCATC 5 cut(s) 88, 166, 642, 684, 735
MaeI CTAG 3 cut(s) 548, 599, 603
MaeIII GTNAC 3 cut(s) 67, 74, 618
MalI GATC 6 cut(s) 149, 189, 251, 477, 513, 555
MboI GATC 6 cut(s) 147, 187, 249, 475, 511, 553
MboII GAAGA 6 cut(s) 293, 350, 353, 356, 485, 576
MfeI CAATTG 1 cut(s) 735
MflI RGATCY 1 cut(s) 475
MluCI AATT 7 cut(s) 137, 240, 258, 293, 628, 717, 735
MlyI GAGTC 1 cut(s) 29
MmeI TCCRAC 1 cut(s) 201
MnlI CCTC 8 cut(s) 121, 154, 283, 304, 313, 370, 568, 679
MseI TTAA 3 cut(s) 113, 459, 741
MslI CAYNNNNRTG 1 cut(s) 50
MspI CCGG 2 cut(s) 180, 191
MspR9I CCNGG 2 cut(s) 181, 704
MunI CAATTG 1 cut(s) 735
Mva1269I GAATGC 1 cut(s) 390
MvaI CCWGG 1 cut(s) 704
NciI CCSGG 1 cut(s) 181
NdeII GATC 6 cut(s) 147, 187, 249, 475, 511, 553
NlaIII CATG 5 cut(s) 128, 211, 311, 367, 386
NspI RCATGY 1 cut(s) 211
OliI CACNNNNGTG 1 cut(s) 50
PctI GAATGC 1 cut(s) 390
PfeI GAWTC 1 cut(s) 224
PleI GAGTC 1 cut(s) 28
PpsI GAGTC 1 cut(s) 28
Psp6I CCWGG 1 cut(s) 702
PspEI GGTNACC 1 cut(s) 74
PspGI CCWGG 1 cut(s) 702
PsuI RGATCY 1 cut(s) 475
RsaI GTAC 1 cut(s) 755
RsaNI GTAC 1 cut(s) 754
RseI CAYNNNNRTG 1 cut(s) 50
SaqAI TTAA 3 cut(s) 113, 459, 741
Sau3AI GATC 6 cut(s) 147, 187, 249, 475, 511, 553
SchI GAGTC 1 cut(s) 29
ScrFI CCNGG 2 cut(s) 181, 704
SetI ASST 9 cut(s) 76, 147, 416, 540, 549, 604, 690, 705, 755
SfaNI GCATC 5 cut(s) 88, 166, 642, 684, 735
SmiMI CAYNNNNRTG 1 cut(s) 50
SmlI CTYRAG 1 cut(s) 395
SmoI CTYRAG 1 cut(s) 395
Sse9I AATT 7 cut(s) 137, 240, 258, 293, 628, 717, 735
SsiI CCGC 2 cut(s) 79, 106
SspI AATATT 3 cut(s) 32, 463, 561
SspMI CTAG 3 cut(s) 548, 599, 603
StyD4I CCNGG 2 cut(s) 179, 702
StyI CCWWGG 3 cut(s) 63, 547, 749
TaqI TCGA 1 cut(s) 14
TasI AATT 7 cut(s) 137, 240, 258, 293, 628, 717, 735
TfiI GAWTC 1 cut(s) 224
Tru1I TTAA 3 cut(s) 113, 459, 741
Tru9I TTAA 3 cut(s) 113, 459, 741
TscAI CASTG 3 cut(s) 52, 138, 427
TspDTI ATGAA 2 cut(s) 17, 294
TspRI CASTG 3 cut(s) 52, 138, 427
XapI RAATTY 3 cut(s) 240, 258, 293
XceI RCATGY 1 cut(s) 211
XmaJI CCTAGG 1 cut(s) 547
XmiI GTMKAC 1 cut(s) 501
XspI CTAG 3 cut(s) 548, 599, 603
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.