Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 475 |
| AccIII | TCCGGA | 1 cut(s) | 24 |
| AciI | CCGC | 1 cut(s) | 161 |
| AfaI | GTAC | 2 cut(s) | 180, 336 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 257, 350, 364 |
| AgsI | TTSAA | 1 cut(s) | 434 |
| AjnI | CCWGG | 2 cut(s) | 170, 253 |
| AluBI | AGCT | 2 cut(s) | 166, 410 |
| AluI | AGCT | 2 cut(s) | 166, 410 |
| Alw21I | GWGCWC | 1 cut(s) | 160 |
| Alw26I | GTCTC | 2 cut(s) | 80, 138 |
| Aor13HI | TCCGGA | 1 cut(s) | 24 |
| ApeKI | GCWGC | 3 cut(s) | 6, 163, 470 |
| AspA2I | CCTAGG | 1 cut(s) | 194 |
| AspLEI | GCGC | 2 cut(s) | 475, 477 |
| AspS9I | GGNCC | 1 cut(s) | 198 |
| AvaII | GGWCC | 1 cut(s) | 198 |
| AvrII | CCTAGG | 1 cut(s) | 194 |
| BanII | GRGCYC | 1 cut(s) | 358 |
| Bbv12I | GWGCWC | 1 cut(s) | 160 |
| BbvI | GCAGC | 3 cut(s) | 18, 175, 457 |
| BccI | CCATC | 1 cut(s) | 379 |
| BciT130I | CCWGG | 2 cut(s) | 172, 255 |
| BclI | TGATCA | 1 cut(s) | 400 |
| BcoDI | GTCTC | 2 cut(s) | 80, 138 |
| BfaI | CTAG | 3 cut(s) | 135, 195, 441 |
| BfmI | CTRYAG | 1 cut(s) | 390 |
| BisI | GCNGC | 3 cut(s) | 7, 164, 471 |
| BlnI | CCTAGG | 1 cut(s) | 194 |
| BlsI | GCNGC | 3 cut(s) | 8, 165, 472 |
| Bme1390I | CCNGG | 2 cut(s) | 172, 255 |
| Bme18I | GGWCC | 1 cut(s) | 198 |
| BmgT120I | GGNCC | 1 cut(s) | 198 |
| BmiI | GGNNCC | 3 cut(s) | 248, 355, 369 |
| BmrFI | CCNGG | 2 cut(s) | 172, 255 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 235, 267 |
| BpmI | CTGGAG | 2 cut(s) | 237, 372 |
| BsaJI | CCNNGG | 3 cut(s) | 151, 170, 194 |
| BsaWI | WCCGGW | 1 cut(s) | 24 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 257, 350, 364 |
| BseAI | TCCGGA | 1 cut(s) | 24 |
| BseBI | CCWGG | 2 cut(s) | 172, 255 |
| BseDI | CCNNGG | 3 cut(s) | 151, 170, 194 |
| BseGI | GGATG | 1 cut(s) | 147 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 257, 350, 364 |
| BseMII | CTCAG | 1 cut(s) | 315 |
| BsePI | GCGCGC | 1 cut(s) | 473 |
| BseXI | GCAGC | 3 cut(s) | 18, 175, 457 |
| BseYI | CCCAGC | 1 cut(s) | 357 |
| Bsh1236I | CGCG | 1 cut(s) | 475 |
| Bsh1285I | CGRYCG | 1 cut(s) | 91 |
| BsiEI | CGRYCG | 1 cut(s) | 91 |
| BsiHKAI | GWGCWC | 1 cut(s) | 160 |
| BsiSI | CCGG | 1 cut(s) | 25 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 257, 350, 364 |
| BsmAI | GTCTC | 2 cut(s) | 80, 138 |
| BsmBI | CGTCTC | 1 cut(s) | 80 |
| Bsp1286I | GDGCHC | 2 cut(s) | 160, 358 |
| Bsp13I | TCCGGA | 1 cut(s) | 24 |
| Bsp143I | GATC | 2 cut(s) | 88, 400 |
| BspACI | CCGC | 1 cut(s) | 161 |
| BspCNI | CTCAG | 1 cut(s) | 316 |
| BspEI | TCCGGA | 1 cut(s) | 24 |
| BspFNI | CGCG | 1 cut(s) | 475 |
| BspHI | TCATGA | 1 cut(s) | 403 |
| BspLI | GGNNCC | 3 cut(s) | 248, 355, 369 |
| BssECI | CCNNGG | 3 cut(s) | 151, 170, 194 |
| BssHII | GCGCGC | 1 cut(s) | 473 |
| BssMI | GATC | 2 cut(s) | 88, 400 |
| BssT1I | CCWWGG | 1 cut(s) | 194 |
| Bst2UI | CCWGG | 2 cut(s) | 172, 255 |
| Bst6I | CTCTTC | 1 cut(s) | 136 |
| BstC8I | GCNNGC | 1 cut(s) | 475 |
| BstDEI | CTNAG | 1 cut(s) | 324 |
| BstDSI | CCRYGG | 1 cut(s) | 151 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 255 |
| BstF5I | GGATG | 1 cut(s) | 147 |
| BstFNI | CGCG | 1 cut(s) | 475 |
| BstHHI | GCGC | 2 cut(s) | 475, 477 |
| BstKTI | GATC | 2 cut(s) | 91, 403 |
| BstMAI | GTCTC | 2 cut(s) | 80, 138 |
| BstMBI | GATC | 2 cut(s) | 88, 400 |
| BstMCI | CGRYCG | 1 cut(s) | 91 |
| BstNI | CCWGG | 2 cut(s) | 172, 255 |
| BstSCI | CCNGG | 2 cut(s) | 170, 253 |
| BstSFI | CTRYAG | 1 cut(s) | 390 |
| BstUI | CGCG | 1 cut(s) | 475 |
| BstV1I | GCAGC | 3 cut(s) | 18, 175, 457 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 261 |
| BtgI | CCRYGG | 1 cut(s) | 151 |
| BtsCI | GGATG | 1 cut(s) | 147 |
| Cac8I | GCNNGC | 1 cut(s) | 475 |
| CciI | TCATGA | 1 cut(s) | 403 |
| CfoI | GCGC | 2 cut(s) | 475, 477 |
| Cfr13I | GGNCC | 1 cut(s) | 198 |
| CseI | GACGC | 1 cut(s) | 62 |
| Csp6I | GTAC | 2 cut(s) | 179, 335 |
| CviAII | CATG | 2 cut(s) | 380, 404 |
| CviJI | RGCY | 8 cut(s) | 40, 166, 216, 247, 356, 410, 444, 470 |
| CviKI_1 | RGCY | 8 cut(s) | 40, 166, 216, 247, 356, 410, 444, 470 |
| CviQI | GTAC | 2 cut(s) | 179, 335 |
| DdeI | CTNAG | 1 cut(s) | 324 |
| DpnI | GATC | 2 cut(s) | 90, 402 |
| DpnII | GATC | 2 cut(s) | 88, 400 |
| Eam1104I | CTCTTC | 1 cut(s) | 136 |
| EarI | CTCTTC | 1 cut(s) | 136 |
| Eco130I | CCWWGG | 1 cut(s) | 194 |
| Eco24I | GRGCYC | 1 cut(s) | 358 |
| Eco47I | GGWCC | 1 cut(s) | 198 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 255 |
| EcoRII | CCWGG | 2 cut(s) | 170, 253 |
| EcoT14I | CCWWGG | 1 cut(s) | 194 |
| EcoT38I | GRGCYC | 1 cut(s) | 358 |
| ErhI | CCWWGG | 1 cut(s) | 194 |
| Esp3I | CGTCTC | 1 cut(s) | 80 |
| FaeI | CATG | 2 cut(s) | 383, 407 |
| FaiI | YATR | 9 cut(s) | 56, 68, 83, 189, 273, 299, 381, 405, 464 |
| FatI | CATG | 2 cut(s) | 379, 403 |
| FbaI | TGATCA | 1 cut(s) | 400 |
| Fnu4HI | GCNGC | 3 cut(s) | 7, 164, 471 |
| FokI | GGATG | 1 cut(s) | 134 |
| FriOI | GRGCYC | 1 cut(s) | 358 |
| Fsp4HI | GCNGC | 3 cut(s) | 7, 164, 471 |
| FspBI | CTAG | 3 cut(s) | 135, 195, 441 |
| GlaI | GCGC | 2 cut(s) | 474, 476 |
| GluI | GCNGC | 3 cut(s) | 7, 164, 471 |
| GsaI | CCCAGC | 1 cut(s) | 361 |
| GsuI | CTGGAG | 2 cut(s) | 237, 372 |
| HapII | CCGG | 1 cut(s) | 25 |
| HgaI | GACGC | 1 cut(s) | 62 |
| HhaI | GCGC | 2 cut(s) | 475, 477 |
| Hin1II | CATG | 2 cut(s) | 383, 407 |
| Hin6I | GCGC | 2 cut(s) | 473, 475 |
| HinP1I | GCGC | 2 cut(s) | 473, 475 |
| HinfI | GANTC | 4 cut(s) | 116, 386, 413, 452 |
| HpaII | CCGG | 1 cut(s) | 25 |
| Hpy166II | GTNNAC | 2 cut(s) | 181, 235 |
| Hpy188I | TCNGA | 1 cut(s) | 457 |
| Hpy188III | TCNNGA | 4 cut(s) | 25, 77, 351, 404 |
| Hpy8I | GTNNAC | 2 cut(s) | 181, 235 |
| HpyCH4IV | ACGT | 1 cut(s) | 97 |
| HpyF3I | CTNAG | 1 cut(s) | 324 |
| HpySE526I | ACGT | 1 cut(s) | 97 |
| Hsp92II | CATG | 2 cut(s) | 383, 407 |
| HspAI | GCGC | 2 cut(s) | 473, 475 |
| Kpn2I | TCCGGA | 1 cut(s) | 24 |
| Ksp22I | TGATCA | 1 cut(s) | 400 |
| Kzo9I | GATC | 2 cut(s) | 88, 400 |
| LmnI | GCTCC | 4 cut(s) | 163, 171, 252, 353 |
| LpnPI | CCDG | 8 cut(s) | 38, 60, 157, 184, 240, 267, 336, 371 |
| Lsp1109I | GCAGC | 3 cut(s) | 18, 175, 457 |
| MaeI | CTAG | 3 cut(s) | 135, 195, 441 |
| MaeII | ACGT | 1 cut(s) | 97 |
| MalI | GATC | 2 cut(s) | 90, 402 |
| MboI | GATC | 2 cut(s) | 88, 400 |
| MboII | GAAGA | 3 cut(s) | 73, 153, 270 |
| MhlI | GDGCHC | 2 cut(s) | 160, 358 |
| MluCI | AATT | 2 cut(s) | 396, 434 |
| MlyI | GAGTC | 3 cut(s) | 125, 395, 461 |
| MnlI | CCTC | 7 cut(s) | 117, 195, 227, 237, 251, 261, 431 |
| MroI | TCCGGA | 1 cut(s) | 24 |
| MseI | TTAA | 2 cut(s) | 101, 484 |
| MspI | CCGG | 1 cut(s) | 25 |
| MspR9I | CCNGG | 2 cut(s) | 172, 255 |
| MvaI | CCWGG | 2 cut(s) | 172, 255 |
| MvnI | CGCG | 1 cut(s) | 475 |
| NdeII | GATC | 2 cut(s) | 88, 400 |
| NlaIII | CATG | 2 cut(s) | 383, 407 |
| NlaIV | GGNNCC | 3 cut(s) | 248, 355, 369 |
| PagI | TCATGA | 1 cut(s) | 403 |
| PauI | GCGCGC | 1 cut(s) | 473 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 84 |
| PfeI | GAWTC | 1 cut(s) | 413 |
| PfoI | TCCNGGA | 1 cut(s) | 253 |
| PkrI | GCNGC | 3 cut(s) | 8, 165, 472 |
| Ple19I | CGATCG | 1 cut(s) | 91 |
| PleI | GAGTC | 3 cut(s) | 124, 394, 460 |
| PpsI | GAGTC | 3 cut(s) | 124, 394, 460 |
| Psp6I | CCWGG | 2 cut(s) | 170, 253 |
| PspFI | CCCAGC | 1 cut(s) | 357 |
| PspGI | CCWGG | 2 cut(s) | 170, 253 |
| PspN4I | GGNNCC | 3 cut(s) | 248, 355, 369 |
| PspPI | GGNCC | 1 cut(s) | 198 |
| PteI | GCGCGC | 1 cut(s) | 473 |
| PvuI | CGATCG | 1 cut(s) | 91 |
| RsaI | GTAC | 2 cut(s) | 180, 336 |
| RsaNI | GTAC | 2 cut(s) | 179, 335 |
| SaqAI | TTAA | 2 cut(s) | 101, 484 |
| SatI | GCNGC | 3 cut(s) | 7, 164, 471 |
| Sau3AI | GATC | 2 cut(s) | 88, 400 |
| Sau96I | GGNCC | 1 cut(s) | 198 |
| SchI | GAGTC | 3 cut(s) | 125, 395, 461 |
| ScrFI | CCNGG | 2 cut(s) | 172, 255 |
| SduI | GDGCHC | 2 cut(s) | 160, 358 |
| SetI | ASST | 7 cut(s) | 100, 128, 168, 200, 289, 369, 412 |
| SfcI | CTRYAG | 1 cut(s) | 390 |
| SinI | GGWCC | 1 cut(s) | 198 |
| Sse9I | AATT | 2 cut(s) | 396, 434 |
| SsiI | CCGC | 1 cut(s) | 161 |
| SspI | AATATT | 1 cut(s) | 430 |
| SspMI | CTAG | 3 cut(s) | 135, 195, 441 |
| StyD4I | CCNGG | 2 cut(s) | 170, 253 |
| StyI | CCWWGG | 1 cut(s) | 194 |
| TaiI | ACGT | 1 cut(s) | 100 |
| TaqI | TCGA | 4 cut(s) | 78, 91, 212, 423 |
| TasI | AATT | 2 cut(s) | 396, 434 |
| TatI | WGTACW | 2 cut(s) | 178, 334 |
| TfiI | GAWTC | 1 cut(s) | 413 |
| Tru1I | TTAA | 2 cut(s) | 101, 484 |
| Tru9I | TTAA | 2 cut(s) | 101, 484 |
| TseI | GCWGC | 3 cut(s) | 6, 163, 470 |
| TspDTI | ATGAA | 1 cut(s) | 420 |
| TspGWI | ACGGA | 1 cut(s) | 140 |
| VpaK11BI | GGWCC | 1 cut(s) | 198 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 255 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 261 |
| XmaJI | CCTAGG | 1 cut(s) | 194 |
| XspI | CTAG | 3 cut(s) | 135, 195, 441 |