Rh5AG414800

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000144
Physical Location & Seq
Forward (+)
71655324 .. 71662883
7560 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG414800.1

Sequence Viewer

Length: 1947 bp
ATGGAGTTCATGATTAAAACTGTATTAGTTTGCGGTTGCTTAGTGTTTGGTGGTGTGGTGATTAAGAGACTATTCCGTCACAAGCCCTGCTGGGAGACACTACCTGACGATATCATGGAGGTTATTGTGCCACACCTGAGTGTACGTGATCGTATACGCTTAAGCCTAGTTTGCAAGCCATGGAGCTCAGTTCCTATGTGCAAAGACATCCCTAGTTCTCCTCACAAACTCCCCTGGTTAGTACTCCCTCAACCTGAATCTCAAGCTCTAACTTGCAATGATACCTCCTTAAGCTTTTACGACCTCCATGACGGCAAAGTGGTTAAGTTGGAGCTGCCTGAACCTGTTCGTGGAGCGTGGTTTCGAGGGTCTTCGAAAGGTTGGTTGATTATGATTAAGGAAAGTGAACAAAACACTACAACATTTCTGGTAAATCCAATTTCAGGAGTCCAACACCAACTTCCATCCTTGAGAACGATTCCGTCTTTCCAGAAGTTTGTAGAAACGAAGAAATGGAAACGATATGGTGCCTTTGCTTTCTTCAACGGCCTTGTGCTATCTACCTCAGATATTAATTCCGATGAATGTGTGGTAGCAGCTGCTCTTGGGTGTGCCAATGATATATTGGGTTTGTGCAGACCTGGAGACAAAGAATGGAATATCTTTCGGTTATTAGATGCAGATGAAGCTTTGACTGATTTACTGTTTTGTTCTGGCAAGCTATACGCCTTGGTTGTGAGTAATAATAAGTCCGGCGTTGTTTCAACTGCTCGTACGTTGAATTTTGGAGATCATTCGGTGGAATTGAAATTAATCTATAACGCAAATATAAACTTCGTTCCTAACTCTGAATATCATGGTAACTATGAGATCTGCGTACAAGGCTATGTTGACTCATATTTGATAGAGTCTGCAACCAATAAGGAAGTATTGTTGATCCACCAAAGGTTAGATGTATTTTCGAGCATAGGTGACAACAACAACGGAGAGAATGGTGGTGGCGGCAACCATGAGGAAGTGGAAGTGGAAGTCGAAGGCAATGACGGTGGTGATGACGCCAATGAGGAATGGAACAACTTGGGAGGAAATGGTGGTGATATAGGTGACTGGGGCATCAATATTGTTGAGGAAGATGATCATTATCTACGTAATGATACATTGATTTATAATTGGGATGAGATTCCTGATGACCTGCTCTCTCAAATCCCAGATGAGTTGTTCAGTCAAGTTCCCAGTCTTGATCAACATACTAGAATGTCAAAGTCTCAATTTTCTTACGAAGTGGAACATGGATTGAATGAAGAAGAGAATTGTGTAATTGAAGAAGAGGAAGGCATGTATGATAATTTTGTGCAAAATGAAGGGGAAGATGATCAATTGCAACTGAACCCTCGATTTGAACATGGAAGTGAGTGTGATGACTCCGATTATGAATTGTATGATGAAGAAAATGATGTTGCCATAGAGGATGATCATTTATTTGATGATACTGCTGTACATGTGTTGACTCCATTAGGGAGACCAAATGTTGAAGATGATGATACTTTGTCACTAAACCTTTCACAAGAAAACACTGTGTTAGAGGATGAATATACCATGGAATCTGCTTCCCTGTTACCATTAAATTTCATGCAAGATAACATTGTCCAAGAGAGAGAATACGATTCAGATGATTTGAACAGTTTAGTGAATTCAGATGATGAAGAAGAAGGAATGATTAAATATCCTGAGTTTAACGCTGCCACAGATATGGAGAACCCCGTATTCGTGATCGGGATGCTGTTTGCCACAAAAAATGATGTGAAGGAAGCAATTAAGGAGTATTCAATCAAAGGGGGTTATGAGACAACTATAAAAAAGAATGACAAGACCCGGATGACTGCAATGTGTATCCCAGGTTGTCCTTGGAAAATTCATGCGTCTCCGAATGAAGAAGGGTTTAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

648

Amino Acids

73.08

Weight (kDa)

4.34

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 99 - 360 6.9e-24 KIB1-4 beta-propeller
DBD_Tnp_Mut PF03108 590 - 644 2.6e-11 MuDR family transposase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1167
AasI GACNNNNNNGTC 1 cut(s) 75
Acc36I ACCTGC 1 cut(s) 1200
AccB1I GGYRCC 1 cut(s) 527
AccI GTMKAC 1 cut(s) 154
AciI CCGC 2 cut(s) 33, 1002
AclWI GGATC 1 cut(s) 931
AcsI RAATTY 4 cut(s) 781, 1624, 1690, 1911
AcyI GRCGYC 1 cut(s) 1056
AfaI GTAC 5 cut(s) 144, 243, 775, 879, 1497
AfiI CCNNNNNNNGG 2 cut(s) 350, 443
AflII CTTAAG 2 cut(s) 160, 289
AflIII ACRYGT 1 cut(s) 1498
AjnI CCWGG 3 cut(s) 233, 640, 1894
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AleI CACNNNNGTG 1 cut(s) 138
AluBI AGCT 7 cut(s) 186, 266, 294, 334, 599, 689, 721
AluI AGCT 7 cut(s) 186, 266, 294, 334, 599, 689, 721
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 7 cut(s) 61, 89, 639, 1269, 1513, 1838, 1926
AlwI GGATC 1 cut(s) 931
AoxI GGCC 1 cut(s) 547
ApeKI GCWGC 4 cut(s) 334, 596, 599, 1739
ApoI RAATTY 4 cut(s) 781, 1624, 1690, 1911
AseI ATTAAT 2 cut(s) 573, 812
Asp700I GAANNNNTTC 1 cut(s) 345
AsuC2I CCSGG 1 cut(s) 1873
AsuHPI GGTGA 5 cut(s) 70, 983, 1061, 1106, 1115
AsuII TTCGAA 1 cut(s) 374
BaeI ACNNNNGTAYC 2 cut(s) 1479, 1512
BanI GGYRCC 1 cut(s) 527
BanII GRGCYC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 4 cut(s) 321, 586, 608, 1726
BccI CCATC 1 cut(s) 472
BceAI ACGGC 2 cut(s) 328, 562
BciT130I CCWGG 3 cut(s) 235, 642, 1896
BciVI GTATCC 1 cut(s) 1901
BclI TGATCA 4 cut(s) 1135, 1240, 1372, 1471
BcnI CCSGG 1 cut(s) 1873
BcoDI GTCTC 7 cut(s) 61, 89, 639, 1269, 1513, 1838, 1926
BfaI CTAG 3 cut(s) 167, 213, 1251
BfrI CTTAAG 2 cut(s) 160, 289
BfuAI ACCTGC 1 cut(s) 1200
BfuI GTATCC 1 cut(s) 1901
BglII AGATCT 1 cut(s) 870
BisI GCNGC 5 cut(s) 335, 597, 600, 1003, 1740
BlsI GCNGC 5 cut(s) 336, 598, 601, 1004, 1741
BmcAI AGTACT 1 cut(s) 243
Bme1390I CCNGG 4 cut(s) 235, 642, 1873, 1896
BmiI GGNNCC 1 cut(s) 529
BmrFI CCNGG 4 cut(s) 235, 642, 1873, 1896
BmrI ACTGGG 2 cut(s) 1117, 1227
BmsI GCATC 3 cut(s) 667, 1122, 1767
BmuI ACTGGG 2 cut(s) 1117, 1227
BpiI GAAGAC 1 cut(s) 363
BpmI CTGGAG 1 cut(s) 663
Bpu14I TTCGAA 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 246, 490
BpuMI CCSGG 1 cut(s) 1873
BsaAI YACGTR 2 cut(s) 146, 1148
BsaBI GATNNNNATC 1 cut(s) 1140
BsaHI GRCGYC 1 cut(s) 1056
BsaI GGTCTC 1 cut(s) 1513
BsaJI CCNNGG 6 cut(s) 179, 233, 729, 1596, 1894, 1904
Bsc4I CCNNNNNNNGG 2 cut(s) 350, 443
Bse1I ACTGG 2 cut(s) 1112, 1233
Bse3DI GCAATG 3 cut(s) 283, 1045, 1890
Bse8I GATNNNNATC 1 cut(s) 1140
BseBI CCWGG 3 cut(s) 235, 642, 1896
BseDI CCNNGG 6 cut(s) 179, 233, 729, 1596, 1894, 1904
BseGI GGATG 7 cut(s) 207, 464, 1180, 1474, 1591, 1782, 1881
BseJI GATNNNNATC 1 cut(s) 1140
BseLI CCNNNNNNNGG 2 cut(s) 350, 443
BseMI GCAATG 3 cut(s) 283, 1045, 1890
BseMII CTCAG 4 cut(s) 128, 201, 579, 1719
BseNI ACTGG 2 cut(s) 1112, 1233
BseRI GAGGAG 1 cut(s) 210
BseXI GCAGC 4 cut(s) 321, 586, 608, 1726
BseYI CCCAGC 1 cut(s) 90
BsgI GTGCAG 1 cut(s) 655
BshFI GGCC 1 cut(s) 549
BshNI GGYRCC 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 188
BsiSI CCGG 2 cut(s) 753, 1873
BsiWI CGTACG 1 cut(s) 773
BslI CCNNNNNNNGG 2 cut(s) 350, 443
BsmAI GTCTC 7 cut(s) 61, 89, 639, 1269, 1513, 1838, 1926
BsmBI CGTCTC 1 cut(s) 1926
BsnI GGCC 1 cut(s) 549
Bso31I GGTCTC 1 cut(s) 1513
Bsp119I TTCGAA 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 188
Bsp1407I TGTACA 1 cut(s) 1495
Bsp143I GATC 9 cut(s) 148, 790, 870, 936, 1135, 1240, 1372, 1471, 1770
Bsp19I CCATGG 2 cut(s) 179, 1596
BspACI CCGC 2 cut(s) 33, 1002
BspANI GGCC 1 cut(s) 549
BspCNI CTCAG 4 cut(s) 129, 200, 578, 1720
BspHI TCATGA 1 cut(s) 9
BspLI GGNNCC 1 cut(s) 529
BspMI ACCTGC 1 cut(s) 1200
BspPI GGATC 1 cut(s) 931
BspT104I TTCGAA 1 cut(s) 374
BspT107I GGYRCC 1 cut(s) 527
BspTI CTTAAG 2 cut(s) 160, 289
BspTNI GGTCTC 1 cut(s) 1513
BsrDI GCAATG 3 cut(s) 283, 1045, 1890
BsrGI TGTACA 1 cut(s) 1495
BsrI ACTGG 2 cut(s) 1112, 1233
BssECI CCNNGG 6 cut(s) 179, 233, 729, 1596, 1894, 1904
BssMI GATC 9 cut(s) 148, 790, 870, 936, 1135, 1240, 1372, 1471, 1770
BssNAI GTATAC 1 cut(s) 155
BssNI GRCGYC 1 cut(s) 1056
BssT1I CCWWGG 4 cut(s) 179, 729, 1596, 1904
Bst1107I GTATAC 1 cut(s) 155
Bst2UI CCWGG 3 cut(s) 235, 642, 1896
Bst4CI ACNGT 5 cut(s) 22, 705, 1046, 1576, 1682
Bst6I CTCTTC 2 cut(s) 1299, 1320
BstACI GRCGYC 1 cut(s) 1056
BstAFI CTTAAG 2 cut(s) 160, 289
BstAUI TGTACA 1 cut(s) 1495
BstBAI YACGTR 2 cut(s) 146, 1148
BstBI TTCGAA 1 cut(s) 374
BstC8I GCNNGC 2 cut(s) 176, 719
BstDEI CTNAG 5 cut(s) 40, 137, 187, 565, 1728
BstDSI CCRYGG 2 cut(s) 179, 1596
BstF5I GGATG 7 cut(s) 207, 464, 1180, 1474, 1591, 1782, 1881
BstKTI GATC 9 cut(s) 151, 793, 873, 939, 1138, 1243, 1375, 1474, 1773
BstMAI GTCTC 7 cut(s) 61, 89, 639, 1269, 1513, 1838, 1926
BstMBI GATC 9 cut(s) 148, 790, 870, 936, 1135, 1240, 1372, 1471, 1770
BstMWI GCNNNNNNNGC 3 cut(s) 171, 686, 882
BstNI CCWGG 3 cut(s) 235, 642, 1896
BstNSI RCATGY 2 cut(s) 1339, 1502
BstSCI CCNGG 4 cut(s) 233, 640, 1871, 1894
BstSNI TACGTA 1 cut(s) 1148
BstV1I GCAGC 4 cut(s) 321, 586, 608, 1726
BstV2I GAAGAC 1 cut(s) 363
BstX2I RGATCY 1 cut(s) 870
BstXI CCANNNNNNTGG 1 cut(s) 1750
BstYI RGATCY 1 cut(s) 870
BstZ17I GTATAC 1 cut(s) 155
BsuI GTATCC 1 cut(s) 1901
BsuRI GGCC 1 cut(s) 549
BtgI CCRYGG 2 cut(s) 179, 1596
BtsCI GGATG 7 cut(s) 207, 464, 1180, 1474, 1591, 1782, 1881
BtsIMutI CAGTG 1 cut(s) 1572
BveI ACCTGC 1 cut(s) 1200
Cac8I GCNNGC 2 cut(s) 176, 719
CciI TCATGA 1 cut(s) 9
CseI GACGC 2 cut(s) 1064, 1908
Csp6I GTAC 5 cut(s) 143, 242, 774, 878, 1496
CspCI CAANNNNNGTGG 2 cut(s) 1027, 1062
CviQI GTAC 5 cut(s) 143, 242, 774, 878, 1496
DdeI CTNAG 5 cut(s) 40, 137, 187, 565, 1728
DpnI GATC 9 cut(s) 150, 792, 872, 938, 1137, 1242, 1374, 1473, 1772
DpnII GATC 9 cut(s) 148, 790, 870, 936, 1135, 1240, 1372, 1471, 1770
DrdI GACNNNNNNGTC 1 cut(s) 75
DseDI GACNNNNNNGTC 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 1299, 1320
EarI CTCTTC 2 cut(s) 1299, 1320
Ecl136II GAGCTC 1 cut(s) 186
Eco105I TACGTA 1 cut(s) 1148
Eco130I CCWWGG 4 cut(s) 179, 729, 1596, 1904
Eco24I GRGCYC 1 cut(s) 188
Eco31I GGTCTC 1 cut(s) 1513
Eco32I GATATC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 186
EcoICRI GAGCTC 1 cut(s) 186
EcoRI GAATTC 1 cut(s) 1690
EcoRII CCWGG 3 cut(s) 233, 640, 1894
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 4 cut(s) 179, 729, 1596, 1904
EcoT38I GRGCYC 1 cut(s) 188
ErhI CCWWGG 4 cut(s) 179, 729, 1596, 1904
Esp3I CGTCTC 1 cut(s) 1926
FbaI TGATCA 4 cut(s) 1135, 1240, 1372, 1471
FblI GTMKAC 1 cut(s) 154
Fnu4HI GCNGC 5 cut(s) 335, 597, 600, 1003, 1740
FokI GGATG 7 cut(s) 194, 451, 1187, 1481, 1598, 1789, 1888
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 5 cut(s) 335, 597, 600, 1003, 1740
FspBI CTAG 3 cut(s) 167, 213, 1251
GluI GCNGC 5 cut(s) 335, 597, 600, 1003, 1740
GsaI CCCAGC 1 cut(s) 94
GsuI CTGGAG 1 cut(s) 663
HaeIII GGCC 1 cut(s) 549
HapII CCGG 2 cut(s) 753, 1873
HgaI GACGC 2 cut(s) 1064, 1908
Hin1I GRCGYC 1 cut(s) 1056
HincII GTYRAC 2 cut(s) 892, 1506
HindII GTYRAC 2 cut(s) 892, 1506
HindIII AAGCTT 2 cut(s) 292, 687
HpaII CCGG 2 cut(s) 753, 1873
HphI GGTGA 5 cut(s) 70, 983, 1061, 1106, 1115
Hpy166II GTNNAC 5 cut(s) 143, 155, 407, 892, 1506
Hpy188I TCNGA 7 cut(s) 568, 580, 850, 1426, 1669, 1696, 1926
Hpy188III TCNNGA 8 cut(s) 10, 444, 490, 1184, 1238, 1727, 1768, 1774
Hpy8I GTNNAC 5 cut(s) 143, 155, 407, 892, 1506
HpyAV CCTTC 6 cut(s) 1028, 1325, 1355, 1703, 1798, 1928
HpyCH4III ACNGT 5 cut(s) 22, 705, 1046, 1576, 1682
HpyCH4IV ACGT 3 cut(s) 145, 776, 1147
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 686, 882
HpyF3I CTNAG 5 cut(s) 40, 137, 187, 565, 1728
HpySE526I ACGT 3 cut(s) 145, 776, 1147
Hsp92I GRCGYC 1 cut(s) 1056
Ksp22I TGATCA 4 cut(s) 1135, 1240, 1372, 1471
Kzo9I GATC 9 cut(s) 148, 790, 870, 936, 1135, 1240, 1372, 1471, 1770
LmnI GCTCC 3 cut(s) 183, 331, 353
Lsp1109I GCAGC 4 cut(s) 321, 586, 608, 1726
LweI GCATC 3 cut(s) 667, 1122, 1767
MaeI CTAG 3 cut(s) 167, 213, 1251
MaeII ACGT 3 cut(s) 145, 776, 1147
MaeIII GTNAC 6 cut(s) 77, 860, 971, 1103, 1548, 1614
MalI GATC 9 cut(s) 150, 792, 872, 938, 1137, 1242, 1374, 1473, 1772
MboI GATC 9 cut(s) 148, 790, 870, 936, 1135, 1240, 1372, 1471, 1770
MfeI CAATTG 1 cut(s) 1376
MflI RGATCY 1 cut(s) 870
MhlI GDGCHC 1 cut(s) 188
MlyI GAGTC 5 cut(s) 456, 887, 917, 1415, 1501
MmeI TCCRAC 2 cut(s) 309, 475
MroXI GAANNNNTTC 1 cut(s) 345
MslI CAYNNNNRTG 3 cut(s) 138, 1407, 1748
MspA1I CMGCKG 1 cut(s) 599
MspCI CTTAAG 2 cut(s) 160, 289
MspI CCGG 2 cut(s) 753, 1873
MspR9I CCNGG 4 cut(s) 235, 642, 1873, 1896
MunI CAATTG 1 cut(s) 1376
MvaI CCWGG 3 cut(s) 235, 642, 1896
MwoI GCNNNNNNNGC 3 cut(s) 171, 686, 882
NciI CCSGG 1 cut(s) 1873
NcoI CCATGG 2 cut(s) 179, 1596
NdeII GATC 9 cut(s) 148, 790, 870, 936, 1135, 1240, 1372, 1471, 1770
NlaIV GGNNCC 1 cut(s) 529
NmuCI GTSAC 4 cut(s) 77, 971, 1103, 1548
NspI RCATGY 2 cut(s) 1339, 1502
NspV TTCGAA 1 cut(s) 374
OliI CACNNNNGTG 1 cut(s) 138
PagI TCATGA 1 cut(s) 9
PciI ACATGT 1 cut(s) 1498
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 5 cut(s) 257, 478, 1180, 1601, 1664
Pfl23II CGTACG 1 cut(s) 773
PkrI GCNGC 5 cut(s) 336, 598, 601, 1004, 1741
PleI GAGTC 5 cut(s) 455, 887, 916, 1415, 1501
PpsI GAGTC 5 cut(s) 455, 887, 916, 1415, 1501
Ppu21I YACGTR 2 cut(s) 146, 1148
PscI ACATGT 1 cut(s) 1498
PshBI ATTAAT 2 cut(s) 573, 812
PsiI TTATAA 1 cut(s) 1167
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 3 cut(s) 233, 640, 1894
PspFI CCCAGC 1 cut(s) 90
PspGI CCWGG 3 cut(s) 233, 640, 1894
PspLI CGTACG 1 cut(s) 773
PspN4I GGNNCC 1 cut(s) 529
PsuI RGATCY 1 cut(s) 870
PvuII CAGCTG 1 cut(s) 599
RsaI GTAC 5 cut(s) 144, 243, 775, 879, 1497
RsaNI GTAC 5 cut(s) 143, 242, 774, 878, 1496
RseI CAYNNNNRTG 3 cut(s) 138, 1407, 1748
SacI GAGCTC 1 cut(s) 188
SatI GCNGC 5 cut(s) 335, 597, 600, 1003, 1740
Sau3AI GATC 9 cut(s) 148, 790, 870, 936, 1135, 1240, 1372, 1471, 1770
ScaI AGTACT 1 cut(s) 243
SchI GAGTC 5 cut(s) 456, 887, 917, 1415, 1501
ScrFI CCNGG 4 cut(s) 235, 642, 1873, 1896
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 3 cut(s) 667, 1122, 1767
SfuI TTCGAA 1 cut(s) 374
SmiMI CAYNNNNRTG 3 cut(s) 138, 1407, 1748
SmlI CTYRAG 4 cut(s) 160, 261, 289, 469
SmoI CTYRAG 4 cut(s) 160, 261, 289, 469
SnaBI TACGTA 1 cut(s) 1148
SsiI CCGC 2 cut(s) 33, 1002
SspI AATATT 1 cut(s) 1120
SspMI CTAG 3 cut(s) 167, 213, 1251
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 4 cut(s) 233, 640, 1871, 1894
StyI CCWWGG 4 cut(s) 179, 729, 1596, 1904
TaaI ACNGT 5 cut(s) 22, 705, 1046, 1576, 1682
TaiI ACGT 3 cut(s) 148, 779, 1150
TaqI TCGA 5 cut(s) 364, 374, 962, 1032, 1393
TatI WGTACW 2 cut(s) 241, 1495
TauI GCSGC 1 cut(s) 1005
TfiI GAWTC 5 cut(s) 257, 478, 1180, 1601, 1664
TscAI CASTG 1 cut(s) 1579
TseFI GTSAC 4 cut(s) 77, 971, 1103, 1548
TseI GCWGC 4 cut(s) 334, 596, 599, 1739
Tsp45I GTSAC 4 cut(s) 77, 971, 1103, 1548
TspGWI ACGGA 3 cut(s) 65, 471, 999
TspRI CASTG 1 cut(s) 1579
Vha464I CTTAAG 2 cut(s) 160, 289
VspI ATTAAT 2 cut(s) 573, 812
XapI RAATTY 4 cut(s) 781, 1624, 1690, 1911
XceI RCATGY 2 cut(s) 1339, 1502
XcmI CCANNNNNNNNNTGG 2 cut(s) 622, 1902
XmiI GTMKAC 1 cut(s) 154
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 3 cut(s) 167, 213, 1251
ZrmI AGTACT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.