Rh4CG021600

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0000144
Physical Location & Seq
Reverse (-)
4272144 .. 4273652
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG021600.1

Sequence Viewer

Length: 915 bp
ATGTTTTCTTTAATCTCTTCGTTAGTTAGTTACTTTAGCCAGAAAATTGAGACACTGGTCGTTTGCGGCTGCTTATTGTTTGGTTGTGTGACGATTCGAAGAATATTCCGTCGCAAACCTACTTGGCAGACACTACCTGCCGATATCTTGGAGATGATTGTACAACGCCTCACAATAGGTGATCGTATACGCGTAAGCAACGTTTCCAAGACTTGGAGGTCCGTTGCTATGCGCAGAGATATCCCTAGTGCTCCACATCAACTCCCCTGGTTAGTAATCCCTCAATCTTCCAACCACTACTTAAGCTTTTTCGACCTATCTGATGGCAAAGTTGGCAAGGTGGAGCTGCCTGAATCAGTACGAGGAGGGTGGTTTCTAGGATCTTGTAAAGGTTGGTTTGTCATTGTCAAGGAAGAAGACCTAAACTCTATGATGTTTCTTGTAAACCCAATTTCAGGAGCCCAACACCAACTCCCACCCTTGAGCACAATTCCATCTTTCAAAAAGTATATAGAAACAACCGAATGGAAATGCGCTGGTGGCTTGGCTTTCTGCAATGGCATTGCTTTATCTACCTCCGATGTTACCTCATGTACGGTAGCTGCATCTTTCGATGGTGATGATAAAACATTGTGCTTTTGCAGACCTGGAGACGATGCGTGGAGTATTTTTGAGGACCAACTTCTGTTTTTGTCGAACACTGGTTCCTTGTCCCTTCCAGCAAGGGATATCAAAGGGTTACTAGGGAATAGCATTTACTTTGCAACAAATAGGATCATGAGTTTTCATTCAAAAACATATATATGTCGGGAGATGGGTGAGTTCTACTTCAACAAAGGAAGAATTAAGCGATCATTTCCAAGTGTTGAGGTGTCACTACGATCTCAACTAAGTTGGTTCTCTCCAAGTTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

304

Amino Acids

34.21

Weight (kDa)

8.9

Isoelectric Point (pI)

45.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 42 - 78 1.3e-07 F-box domain
F-box-like PF12937 42 - 78 4.8e-06 F-box-like
Beta-prop_KIB1-4 PF03478 103 - 224 9.2e-18 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 217
Acc16I TGCGCA 1 cut(s) 233
Acc36I ACCTGC 1 cut(s) 145
AccB7I CCANNNNNTGG 1 cut(s) 213
AccI GTMKAC 1 cut(s) 187
AccII CGCG 1 cut(s) 192
AciI CCGC 1 cut(s) 66
AclI AACGTT 1 cut(s) 201
AclWI GGATC 2 cut(s) 388, 782
AfaI GTAC 3 cut(s) 162, 360, 595
AfiI CCNNNNNNNGG 2 cut(s) 213, 455
AflII CTTAAG 1 cut(s) 301
AflIII ACRYGT 1 cut(s) 190
AgsI TTSAA 3 cut(s) 502, 792, 832
AjnI CCWGG 2 cut(s) 266, 646
AloI GAACNNNNNNTCC 2 cut(s) 689, 721
AluBI AGCT 3 cut(s) 306, 346, 602
AluI AGCT 3 cut(s) 306, 346, 602
Alw21I GWGCWC 2 cut(s) 253, 488
Alw26I GTCTC 2 cut(s) 44, 645
AlwI GGATC 2 cut(s) 388, 782
ApeKI GCWGC 3 cut(s) 69, 346, 602
AspLEI GCGC 2 cut(s) 234, 536
AspS9I GGNCC 2 cut(s) 219, 676
AsuHPI GGTGA 3 cut(s) 191, 629, 830
AsuII TTCGAA 1 cut(s) 97
AvaII GGWCC 2 cut(s) 219, 676
BanII GRGCYC 1 cut(s) 463
BbsI GAAGAC 1 cut(s) 423
Bbv12I GWGCWC 2 cut(s) 253, 488
BbvI GCAGC 3 cut(s) 56, 333, 589
BccI CCATC 4 cut(s) 317, 502, 608, 808
BciT130I CCWGG 2 cut(s) 268, 648
BcoDI GTCTC 2 cut(s) 44, 645
BfaI CTAG 3 cut(s) 246, 377, 743
BfrI CTTAAG 1 cut(s) 301
BfuAI ACCTGC 1 cut(s) 145
BisI GCNGC 4 cut(s) 67, 70, 347, 603
BlsI GCNGC 4 cut(s) 68, 71, 348, 604
Bme1390I CCNGG 2 cut(s) 268, 648
Bme18I GGWCC 2 cut(s) 219, 676
BmgT120I GGNCC 2 cut(s) 219, 676
BmiI GGNNCC 2 cut(s) 460, 706
BmrFI CCNGG 2 cut(s) 268, 648
BmsI GCATC 2 cut(s) 614, 646
BoxI GACNNNNGTC 1 cut(s) 56
BpiI GAAGAC 1 cut(s) 423
BpmI CTGGAG 1 cut(s) 669
Bpu14I TTCGAA 1 cut(s) 97
BpuEI CTTGAG 1 cut(s) 502
BsaJI CCNNGG 1 cut(s) 266
BsaXI ACNNNNNCTCC 4 cut(s) 246, 276, 456, 486
Bsc4I CCNNNNNNNGG 2 cut(s) 213, 455
Bse1I ACTGG 2 cut(s) 60, 706
Bse3DI GCAATG 2 cut(s) 561, 562
BseBI CCWGG 2 cut(s) 268, 648
BseDI CCNNGG 1 cut(s) 266
BseLI CCNNNNNNNGG 2 cut(s) 213, 455
BseMI GCAATG 2 cut(s) 561, 562
BseNI ACTGG 2 cut(s) 60, 706
BseRI GAGGAG 1 cut(s) 378
BseXI GCAGC 3 cut(s) 56, 333, 589
Bsh1236I CGCG 1 cut(s) 192
BsiHKAI GWGCWC 2 cut(s) 253, 488
BslFI GGGAC 1 cut(s) 697
BslI CCNNNNNNNGG 2 cut(s) 213, 455
BsmAI GTCTC 2 cut(s) 44, 645
BsmBI CGTCTC 1 cut(s) 645
BsmFI GGGAC 1 cut(s) 697
Bsp119I TTCGAA 1 cut(s) 97
Bsp1286I GDGCHC 3 cut(s) 253, 463, 488
Bsp1407I TGTACA 1 cut(s) 160
Bsp143I GATC 5 cut(s) 181, 380, 774, 851, 881
BspACI CCGC 1 cut(s) 66
BspFNI CGCG 1 cut(s) 192
BspHI TCATGA 1 cut(s) 777
BspLI GGNNCC 2 cut(s) 460, 706
BspMI ACCTGC 1 cut(s) 145
BspPI GGATC 2 cut(s) 388, 782
BspT104I TTCGAA 1 cut(s) 97
BspTI CTTAAG 1 cut(s) 301
BsrDI GCAATG 2 cut(s) 561, 562
BsrGI TGTACA 1 cut(s) 160
BsrI ACTGG 2 cut(s) 60, 706
BssECI CCNNGG 1 cut(s) 266
BssMI GATC 5 cut(s) 181, 380, 774, 851, 881
BssNAI GTATAC 1 cut(s) 188
Bst1107I GTATAC 1 cut(s) 188
Bst2UI CCWGG 2 cut(s) 268, 648
Bst4CI ACNGT 1 cut(s) 598
Bst6I CTCTTC 1 cut(s) 22
BstAFI CTTAAG 1 cut(s) 301
BstAUI TGTACA 1 cut(s) 160
BstBI TTCGAA 1 cut(s) 97
BstDEI CTNAG 1 cut(s) 890
BstFNI CGCG 1 cut(s) 192
BstHHI GCGC 2 cut(s) 234, 536
BstKTI GATC 5 cut(s) 184, 383, 777, 854, 884
BstMAI GTCTC 2 cut(s) 44, 645
BstMBI GATC 5 cut(s) 181, 380, 774, 851, 881
BstMWI GCNNNNNNNGC 2 cut(s) 333, 540
BstNI CCWGG 2 cut(s) 268, 648
BstPAI GACNNNNGTC 1 cut(s) 56
BstSCI CCNGG 2 cut(s) 266, 646
BstUI CGCG 1 cut(s) 192
BstV1I GCAGC 3 cut(s) 56, 333, 589
BstV2I GAAGAC 1 cut(s) 423
BstX2I RGATCY 1 cut(s) 380
BstYI RGATCY 1 cut(s) 380
BstZ17I GTATAC 1 cut(s) 188
BtsIMutI CAGTG 2 cut(s) 53, 699
BveI ACCTGC 1 cut(s) 145
CciI TCATGA 1 cut(s) 777
CfoI GCGC 2 cut(s) 234, 536
Cfr13I GGNCC 2 cut(s) 219, 676
Csp6I GTAC 3 cut(s) 161, 359, 594
CviAII CATG 2 cut(s) 591, 778
CviJI RGCY 8 cut(s) 39, 69, 306, 346, 461, 543, 548, 602
CviKI_1 RGCY 8 cut(s) 39, 69, 306, 346, 461, 543, 548, 602
CviQI GTAC 3 cut(s) 161, 359, 594
DdeI CTNAG 1 cut(s) 890
DpnI GATC 5 cut(s) 183, 382, 776, 853, 883
DpnII GATC 5 cut(s) 181, 380, 774, 851, 881
DrdI GACNNNNNNGTC 1 cut(s) 217
DseDI GACNNNNNNGTC 1 cut(s) 217
Eam1104I CTCTTC 1 cut(s) 22
EarI CTCTTC 1 cut(s) 22
Eco24I GRGCYC 1 cut(s) 463
Eco32I GATATC 3 cut(s) 145, 241, 730
Eco47I GGWCC 2 cut(s) 219, 676
EcoRII CCWGG 2 cut(s) 266, 646
EcoRV GATATC 3 cut(s) 145, 241, 730
EcoT38I GRGCYC 1 cut(s) 463
Esp3I CGTCTC 1 cut(s) 645
FaeI CATG 2 cut(s) 594, 781
FaqI GGGAC 1 cut(s) 697
FatI CATG 2 cut(s) 590, 777
FblI GTMKAC 1 cut(s) 187
Fnu4HI GCNGC 4 cut(s) 67, 70, 347, 603
FriOI GRGCYC 1 cut(s) 463
Fsp4HI GCNGC 4 cut(s) 67, 70, 347, 603
FspBI CTAG 3 cut(s) 246, 377, 743
FspI TGCGCA 1 cut(s) 233
GlaI GCGC 2 cut(s) 233, 535
GluI GCNGC 4 cut(s) 67, 70, 347, 603
GsuI CTGGAG 1 cut(s) 669
HhaI GCGC 2 cut(s) 234, 536
Hin1II CATG 2 cut(s) 594, 781
Hin6I GCGC 2 cut(s) 232, 534
HinP1I GCGC 2 cut(s) 232, 534
HindIII AAGCTT 1 cut(s) 304
HinfI GANTC 2 cut(s) 94, 353
HphI GGTGA 3 cut(s) 191, 629, 830
Hpy166II GTNNAC 2 cut(s) 188, 445
Hpy188I TCNGA 2 cut(s) 322, 580
Hpy188III TCNNGA 3 cut(s) 456, 778, 809
Hpy8I GTNNAC 2 cut(s) 188, 445
Hpy99I CGWCG 1 cut(s) 114
HpyAV CCTTC 1 cut(s) 725
HpyCH4III ACNGT 1 cut(s) 598
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 4 cut(s) 555, 605, 642, 764
HpyF10VI GCNNNNNNNGC 2 cut(s) 333, 540
HpyF3I CTNAG 1 cut(s) 890
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 2 cut(s) 594, 781
HspAI GCGC 2 cut(s) 232, 534
Kzo9I GATC 5 cut(s) 181, 380, 774, 851, 881
LmnI GCTCC 3 cut(s) 256, 343, 458
Lsp1109I GCAGC 3 cut(s) 56, 333, 589
LweI GCATC 2 cut(s) 614, 646
MaeI CTAG 3 cut(s) 246, 377, 743
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 5 cut(s) 29, 88, 583, 738, 873
MalI GATC 5 cut(s) 183, 382, 776, 853, 883
MboI GATC 5 cut(s) 181, 380, 774, 851, 881
MboII GAAGA 6 cut(s) 9, 111, 279, 425, 428, 852
MflI RGATCY 1 cut(s) 380
MhlI GDGCHC 3 cut(s) 253, 463, 488
MluCI AATT 4 cut(s) 45, 450, 489, 843
MluI ACGCGT 1 cut(s) 190
MmeI TCCRAC 1 cut(s) 315
MnlI CCTC 9 cut(s) 179, 210, 291, 356, 359, 586, 598, 667, 862
MseI TTAA 3 cut(s) 11, 302, 846
MslI CAYNNNNRTG 1 cut(s) 802
MspCI CTTAAG 1 cut(s) 301
MspR9I CCNGG 2 cut(s) 268, 648
MvaI CCWGG 2 cut(s) 268, 648
MvnI CGCG 1 cut(s) 192
MwoI GCNNNNNNNGC 2 cut(s) 333, 540
NdeII GATC 5 cut(s) 181, 380, 774, 851, 881
NlaIII CATG 2 cut(s) 594, 781
NlaIV GGNNCC 2 cut(s) 460, 706
NmuCI GTSAC 2 cut(s) 88, 873
NsbI TGCGCA 1 cut(s) 233
NspV TTCGAA 1 cut(s) 97
PagI TCATGA 1 cut(s) 777
PfeI GAWTC 2 cut(s) 94, 353
PflMI CCANNNNNTGG 1 cut(s) 213
PkrI GCNGC 4 cut(s) 68, 71, 348, 604
PshAI GACNNNNGTC 1 cut(s) 56
Psp1406I AACGTT 1 cut(s) 201
Psp6I CCWGG 2 cut(s) 266, 646
PspGI CCWGG 2 cut(s) 266, 646
PspN4I GGNNCC 2 cut(s) 460, 706
PspPI GGNCC 2 cut(s) 219, 676
PsuI RGATCY 1 cut(s) 380
RsaI GTAC 3 cut(s) 162, 360, 595
RsaNI GTAC 3 cut(s) 161, 359, 594
RseI CAYNNNNRTG 1 cut(s) 802
SaqAI TTAA 3 cut(s) 11, 302, 846
SatI GCNGC 4 cut(s) 67, 70, 347, 603
Sau3AI GATC 5 cut(s) 181, 380, 774, 851, 881
Sau96I GGNCC 2 cut(s) 219, 676
ScrFI CCNGG 2 cut(s) 268, 648
SduI GDGCHC 3 cut(s) 253, 463, 488
SfaNI GCATC 2 cut(s) 614, 646
SfuI TTCGAA 1 cut(s) 97
SinI GGWCC 2 cut(s) 219, 676
SmiMI CAYNNNNRTG 1 cut(s) 802
SmlI CTYRAG 2 cut(s) 301, 481
SmoI CTYRAG 2 cut(s) 301, 481
Sse9I AATT 4 cut(s) 45, 450, 489, 843
SsiI CCGC 1 cut(s) 66
SspI AATATT 1 cut(s) 105
SspMI CTAG 3 cut(s) 246, 377, 743
StyD4I CCNGG 2 cut(s) 266, 646
TaaI ACNGT 1 cut(s) 598
TaiI ACGT 1 cut(s) 204
TaqI TCGA 4 cut(s) 97, 312, 612, 695
TasI AATT 4 cut(s) 45, 450, 489, 843
TatI WGTACW 1 cut(s) 160
TauI GCSGC 1 cut(s) 69
TfiI GAWTC 2 cut(s) 94, 353
Tru1I TTAA 3 cut(s) 11, 302, 846
Tru9I TTAA 3 cut(s) 11, 302, 846
TscAI CASTG 2 cut(s) 60, 706
TseFI GTSAC 2 cut(s) 88, 873
TseI GCWGC 3 cut(s) 69, 346, 602
Tsp45I GTSAC 2 cut(s) 88, 873
TspDTI ATGAA 1 cut(s) 776
TspGWI ACGGA 2 cut(s) 98, 211
TspRI CASTG 2 cut(s) 60, 706
Van91I CCANNNNNTGG 1 cut(s) 213
Vha464I CTTAAG 1 cut(s) 301
VpaK11BI GGWCC 2 cut(s) 219, 676
XmiI GTMKAC 1 cut(s) 187
XspI CTAG 3 cut(s) 246, 377, 743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.