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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AcsI | RAATTY | 1 cut(s) | 26 |
| AoxI | GGCC | 1 cut(s) | 37 |
| ApoI | RAATTY | 1 cut(s) | 26 |
| BpuEI | CTTGAG | 1 cut(s) | 149 |
| BseGI | GGATG | 2 cut(s) | 10, 91 |
| BseMII | CTCAG | 1 cut(s) | 166 |
| BseRI | GAGGAG | 1 cut(s) | 125 |
| BshFI | GGCC | 1 cut(s) | 39 |
| BsmI | GAATGC | 1 cut(s) | 123 |
| BsnI | GGCC | 1 cut(s) | 39 |
| BspANI | GGCC | 1 cut(s) | 39 |
| BspCNI | CTCAG | 1 cut(s) | 165 |
| BstDEI | CTNAG | 1 cut(s) | 152 |
| BstF5I | GGATG | 2 cut(s) | 10, 91 |
| BsuRI | GGCC | 1 cut(s) | 39 |
| BtsCI | GGATG | 2 cut(s) | 10, 91 |
| BtsIMutI | CAGTG | 1 cut(s) | 160 |
| CviAII | CATG | 3 cut(s) | 41, 97, 116 |
| CviJI | RGCY | 2 cut(s) | 39, 137 |
| CviKI_1 | RGCY | 2 cut(s) | 39, 137 |
| DdeI | CTNAG | 1 cut(s) | 152 |
| FaeI | CATG | 3 cut(s) | 44, 100, 119 |
| FaiI | YATR | 5 cut(s) | 32, 42, 98, 117, 162 |
| FatI | CATG | 3 cut(s) | 40, 96, 115 |
| FokI | GGATG | 2 cut(s) | 17, 98 |
| HaeIII | GGCC | 1 cut(s) | 39 |
| Hin1II | CATG | 3 cut(s) | 44, 100, 119 |
| Hpy188III | TCNNGA | 1 cut(s) | 128 |
| HpyAV | CCTTC | 3 cut(s) | 29, 50, 74 |
| HpyF3I | CTNAG | 1 cut(s) | 152 |
| Hsp92II | CATG | 3 cut(s) | 44, 100, 119 |
| LmnI | GCTCC | 1 cut(s) | 112 |
| LpnPI | CCDG | 1 cut(s) | 123 |
| MboII | GAAGA | 5 cut(s) | 26, 83, 86, 89, 113 |
| MluCI | AATT | 1 cut(s) | 26 |
| MnlI | CCTC | 4 cut(s) | 16, 37, 46, 103 |
| MseI | TTAA | 1 cut(s) | 193 |
| Mva1269I | GAATGC | 1 cut(s) | 123 |
| NlaIII | CATG | 3 cut(s) | 44, 100, 119 |
| PctI | GAATGC | 1 cut(s) | 123 |
| SaqAI | TTAA | 1 cut(s) | 193 |
| SgeI | CNNG | 6 cut(s) | 53, 64, 109, 128, 140, 150 |
| SmlI | CTYRAG | 1 cut(s) | 128 |
| SmoI | CTYRAG | 1 cut(s) | 128 |
| Sse9I | AATT | 1 cut(s) | 26 |
| TasI | AATT | 1 cut(s) | 26 |
| Tru1I | TTAA | 1 cut(s) | 193 |
| Tru9I | TTAA | 1 cut(s) | 193 |
| TscAI | CASTG | 1 cut(s) | 160 |
| TspDTI | ATGAA | 1 cut(s) | 27 |
| TspRI | CASTG | 1 cut(s) | 160 |
| XapI | RAATTY | 1 cut(s) | 26 |