Protein of unknown function (DUF295)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccI | GTMKAC | 2 cut(s) | 43, 69 |
| AciI | CCGC | 2 cut(s) | 166, 193 |
| AclWI | GGATC | 1 cut(s) | 269 |
| AcsI | RAATTY | 1 cut(s) | 327 |
| AgsI | TTSAA | 3 cut(s) | 83, 229, 337 |
| AleI | CACNNNNGTG | 1 cut(s) | 137 |
| AluBI | AGCT | 3 cut(s) | 35, 60, 232 |
| AluI | AGCT | 3 cut(s) | 35, 60, 232 |
| Alw26I | GTCTC | 1 cut(s) | 45 |
| AlwI | GGATC | 1 cut(s) | 269 |
| ApeKI | GCWGC | 1 cut(s) | 8 |
| ApoI | RAATTY | 1 cut(s) | 327 |
| BbvI | GCAGC | 1 cut(s) | 20 |
| BcoDI | GTCTC | 1 cut(s) | 45 |
| BfaI | CTAG | 2 cut(s) | 32, 48 |
| BisI | GCNGC | 1 cut(s) | 9 |
| BlsI | GCNGC | 1 cut(s) | 10 |
| BmsI | GCATC | 2 cut(s) | 175, 253 |
| BpmI | CTGGAG | 1 cut(s) | 82 |
| BsaJI | CCNNGG | 1 cut(s) | 150 |
| BsaWI | WCCGGW | 1 cut(s) | 277 |
| Bse1I | ACTGG | 1 cut(s) | 139 |
| Bse3DI | GCAATG | 1 cut(s) | 175 |
| BseDI | CCNNGG | 1 cut(s) | 150 |
| BseMI | GCAATG | 1 cut(s) | 175 |
| BseNI | ACTGG | 1 cut(s) | 139 |
| BseRI | GAGGAG | 1 cut(s) | 263 |
| BseXI | GCAGC | 1 cut(s) | 20 |
| BsiSI | CCGG | 1 cut(s) | 278 |
| BsmAI | GTCTC | 1 cut(s) | 45 |
| Bsp143I | GATC | 2 cut(s) | 234, 274 |
| BspACI | CCGC | 2 cut(s) | 166, 193 |
| BspPI | GGATC | 1 cut(s) | 269 |
| BsrDI | GCAATG | 1 cut(s) | 175 |
| BsrI | ACTGG | 1 cut(s) | 139 |
| BssECI | CCNNGG | 1 cut(s) | 150 |
| BssMI | GATC | 2 cut(s) | 234, 274 |
| BssT1I | CCWWGG | 1 cut(s) | 150 |
| BstC8I | GCNNGC | 1 cut(s) | 140 |
| BstEII | GGTNACC | 1 cut(s) | 161 |
| BstKTI | GATC | 2 cut(s) | 237, 277 |
| BstMAI | GTCTC | 1 cut(s) | 45 |
| BstMBI | GATC | 2 cut(s) | 234, 274 |
| BstNSI | RCATGY | 1 cut(s) | 298 |
| BstPI | GGTNACC | 1 cut(s) | 161 |
| BstV1I | GCAGC | 1 cut(s) | 20 |
| BtsI | GCAGTG | 1 cut(s) | 225 |
| BtsIMutI | CAGTG | 2 cut(s) | 132, 225 |
| Cac8I | GCNNGC | 1 cut(s) | 140 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 157, 192 |
| CviAII | CATG | 1 cut(s) | 295 |
| CviJI | RGCY | 4 cut(s) | 35, 60, 232, 286 |
| CviKI_1 | RGCY | 4 cut(s) | 35, 60, 232, 286 |
| DpnI | GATC | 2 cut(s) | 236, 276 |
| DpnII | GATC | 2 cut(s) | 234, 274 |
| DraI | TTTAAA | 1 cut(s) | 201 |
| Eco130I | CCWWGG | 1 cut(s) | 150 |
| Eco91I | GGTNACC | 1 cut(s) | 161 |
| EcoO65I | GGTNACC | 1 cut(s) | 161 |
| EcoT14I | CCWWGG | 1 cut(s) | 150 |
| ErhI | CCWWGG | 1 cut(s) | 150 |
| FaeI | CATG | 1 cut(s) | 298 |
| FaiI | YATR | 9 cut(s) | 6, 18, 145, 147, 240, 245, 296, 301, 342 |
| FatI | CATG | 1 cut(s) | 294 |
| FblI | GTMKAC | 2 cut(s) | 43, 69 |
| Fnu4HI | GCNGC | 1 cut(s) | 9 |
| Fsp4HI | GCNGC | 1 cut(s) | 9 |
| FspBI | CTAG | 2 cut(s) | 32, 48 |
| GluI | GCNGC | 1 cut(s) | 9 |
| GsuI | CTGGAG | 1 cut(s) | 82 |
| HapII | CCGG | 1 cut(s) | 278 |
| Hin1II | CATG | 1 cut(s) | 298 |
| HincII | GTYRAC | 1 cut(s) | 112 |
| HindII | GTYRAC | 1 cut(s) | 112 |
| HinfI | GANTC | 2 cut(s) | 108, 311 |
| HpaII | CCGG | 1 cut(s) | 278 |
| Hpy166II | GTNNAC | 3 cut(s) | 44, 70, 112 |
| Hpy188I | TCNGA | 1 cut(s) | 209 |
| Hpy8I | GTNNAC | 3 cut(s) | 44, 70, 112 |
| HpyCH4V | TGCA | 4 cut(s) | 8, 188, 266, 294 |
| Hsp92II | CATG | 1 cut(s) | 298 |
| Kzo9I | GATC | 2 cut(s) | 234, 274 |
| LpnPI | CCDG | 5 cut(s) | 46, 120, 144, 252, 291 |
| Lsp1109I | GCAGC | 1 cut(s) | 20 |
| LweI | GCATC | 2 cut(s) | 175, 253 |
| MaeI | CTAG | 2 cut(s) | 32, 48 |
| MaeIII | GTNAC | 2 cut(s) | 154, 161 |
| MalI | GATC | 2 cut(s) | 236, 276 |
| MboI | GATC | 2 cut(s) | 234, 274 |
| MboII | GAAGA | 1 cut(s) | 95 |
| MluCI | AATT | 2 cut(s) | 224, 327 |
| MlyI | GAGTC | 1 cut(s) | 117 |
| MmeI | TCCRAC | 1 cut(s) | 288 |
| MnlI | CCTC | 2 cut(s) | 208, 241 |
| MseI | TTAA | 1 cut(s) | 200 |
| MslI | CAYNNNNRTG | 2 cut(s) | 21, 137 |
| MspI | CCGG | 1 cut(s) | 278 |
| NdeII | GATC | 2 cut(s) | 234, 274 |
| NlaIII | CATG | 1 cut(s) | 298 |
| NspI | RCATGY | 1 cut(s) | 298 |
| OliI | CACNNNNGTG | 1 cut(s) | 137 |
| PfeI | GAWTC | 1 cut(s) | 311 |
| PkrI | GCNGC | 1 cut(s) | 10 |
| PleI | GAGTC | 1 cut(s) | 116 |
| PpsI | GAGTC | 1 cut(s) | 116 |
| PspEI | GGTNACC | 1 cut(s) | 161 |
| RseI | CAYNNNNRTG | 2 cut(s) | 21, 137 |
| SaqAI | TTAA | 1 cut(s) | 200 |
| SatI | GCNGC | 1 cut(s) | 9 |
| Sau3AI | GATC | 2 cut(s) | 234, 274 |
| SchI | GAGTC | 1 cut(s) | 117 |
| SetI | ASST | 5 cut(s) | 37, 49, 62, 163, 234 |
| SfaNI | GCATC | 2 cut(s) | 175, 253 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 21, 137 |
| Sse9I | AATT | 2 cut(s) | 224, 327 |
| SsiI | CCGC | 2 cut(s) | 166, 193 |
| SspI | AATATT | 1 cut(s) | 120 |
| SspMI | CTAG | 2 cut(s) | 32, 48 |
| StyI | CCWWGG | 1 cut(s) | 150 |
| TasI | AATT | 2 cut(s) | 224, 327 |
| TfiI | GAWTC | 1 cut(s) | 311 |
| Tru1I | TTAA | 1 cut(s) | 200 |
| Tru9I | TTAA | 1 cut(s) | 200 |
| TscAI | CASTG | 2 cut(s) | 139, 225 |
| TseI | GCWGC | 1 cut(s) | 8 |
| TspDTI | ATGAA | 2 cut(s) | 5, 105 |
| TspRI | CASTG | 2 cut(s) | 139, 225 |
| XapI | RAATTY | 1 cut(s) | 327 |
| XceI | RCATGY | 1 cut(s) | 298 |
| XmiI | GTMKAC | 2 cut(s) | 43, 69 |
| XspI | CTAG | 2 cut(s) | 32, 48 |