RLG00000014599

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0000144
Physical Location & Seq
Reverse (-)
55185440 .. 55186620
1181 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000014599

Sequence Viewer

Length: 540 bp
ATGGTGACTTCAACGTACTCATATTTGGTAGAATCAGCAACAAATAGCGACAAGGAAATTTTGTTGATCCATCAGATGCTCGATTCTTTACTCAACTATGTTGATGATGATGATGATGATGAGTCGGATATGGAATATCAGAGGACAAGAGGGTTCGCAGTATACAAAATTGACCTCGAGTCTGGCAACTTTCATGAGGTACAAAATTTGGGGGACCAAATAATATTTTTGGCAGAACATAGCTCTTCCTTGTCCTTTCCGGGGAATGCCTTCCCAAAAAGTAGACTTTCATCTGATTATGAGCAACTGGCAAACAAATCAGTACACTATGCTCAAGGACTTCCATTAGCTCTTGTGCTTTTCGGTTCACTTCTTTGTGGTAAACATTTGGATCAATGGCAAACAGTTTTATCAGTGTTAGATAATAAGAAAAGAGTTTCTGACAAGAAGATTTTAGAGGATCTGAAAATAACTTGTACTGCATTAGAAGAATGGAAAACTGTGTTGGGTGTATTTGATAAGAGAGTTCCTACTTCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 map04014
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

180

Amino Acids

20.26

Weight (kDa)

4.77

Isoelectric Point (pI)

33.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 7 - 93 2.4e-09 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 162, 283
AclWI GGATC 3 cut(s) 61, 399, 468
AcsI RAATTY 2 cut(s) 57, 205
AfaI GTAC 4 cut(s) 17, 201, 324, 478
AfiI CCNNNNNNNGG 1 cut(s) 261
AgsI TTSAA 1 cut(s) 12
AhdI GACNNNNNGTC 1 cut(s) 178
AjuI GAANNNNNNNTTGG 2 cut(s) 488, 520
AluBI AGCT 2 cut(s) 243, 350
AluI AGCT 2 cut(s) 243, 350
AlwI GGATC 3 cut(s) 61, 399, 468
Ama87I CYCGRG 1 cut(s) 176
ApoI RAATTY 2 cut(s) 57, 205
Asp700I GAANNNNTTC 1 cut(s) 269
AspS9I GGNCC 1 cut(s) 214
AsuC2I CCSGG 1 cut(s) 261
AsuHPI GGTGA 1 cut(s) 16
AvaI CYCGRG 1 cut(s) 176
AvaII GGWCC 1 cut(s) 214
BccI CCATC 1 cut(s) 78
BcnI CCSGG 1 cut(s) 261
BfaI CTAG 1 cut(s) 538
Bme1390I CCNGG 1 cut(s) 261
Bme18I GGWCC 1 cut(s) 214
BmeRI GACNNNNNGTC 1 cut(s) 178
BmeT110I CYCGRG 1 cut(s) 176
BmgT120I GGNCC 1 cut(s) 214
BmiI GGNNCC 1 cut(s) 215
BmrFI CCNGG 1 cut(s) 261
BmsI GCATC 1 cut(s) 66
BpuEI CTTGAG 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 261
BsaJI CCNNGG 1 cut(s) 260
Bsc4I CCNNNNNNNGG 1 cut(s) 261
Bse1I ACTGG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 260
BseLI CCNNNNNNNGG 1 cut(s) 261
BseNI ACTGG 1 cut(s) 312
BsiHKCI CYCGRG 1 cut(s) 176
BsiSI CCGG 1 cut(s) 260
BslFI GGGAC 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 261
BsmFI GGGAC 1 cut(s) 227
BsmI GAATGC 1 cut(s) 271
BsoBI CYCGRG 1 cut(s) 176
Bsp143I GATC 3 cut(s) 66, 391, 460
BspHI TCATGA 1 cut(s) 193
BspLI GGNNCC 1 cut(s) 215
BspPI GGATC 3 cut(s) 61, 399, 468
BspQI GCTCTTC 1 cut(s) 250
BsrI ACTGG 1 cut(s) 312
BssECI CCNNGG 1 cut(s) 260
BssMI GATC 3 cut(s) 66, 391, 460
BssNAI GTATAC 1 cut(s) 163
Bst1107I GTATAC 1 cut(s) 163
Bst4CI ACNGT 2 cut(s) 406, 502
Bst6I CTCTTC 1 cut(s) 250
BstKTI GATC 3 cut(s) 69, 394, 463
BstMBI GATC 3 cut(s) 66, 391, 460
BstSCI CCNGG 1 cut(s) 259
BstX2I RGATCY 1 cut(s) 460
BstYI RGATCY 1 cut(s) 460
BstZ17I GTATAC 1 cut(s) 163
BtsIMutI CAGTG 1 cut(s) 420
CciI TCATGA 1 cut(s) 193
Cfr13I GGNCC 1 cut(s) 214
Csp6I GTAC 4 cut(s) 16, 200, 323, 477
CviAII CATG 1 cut(s) 194
CviJI RGCY 2 cut(s) 243, 350
CviKI_1 RGCY 2 cut(s) 243, 350
CviQI GTAC 4 cut(s) 16, 200, 323, 477
DpnI GATC 3 cut(s) 68, 393, 462
DpnII GATC 3 cut(s) 66, 391, 460
DriI GACNNNNNGTC 1 cut(s) 178
Eam1104I CTCTTC 1 cut(s) 250
Eam1105I GACNNNNNGTC 1 cut(s) 178
EarI CTCTTC 1 cut(s) 250
Eco47I GGWCC 1 cut(s) 214
Eco88I CYCGRG 1 cut(s) 176
FaeI CATG 1 cut(s) 197
FaiI YATR 8 cut(s) 22, 99, 131, 163, 195, 240, 300, 330
FaqI GGGAC 1 cut(s) 227
FatI CATG 1 cut(s) 193
FblI GTMKAC 2 cut(s) 162, 283
FspBI CTAG 1 cut(s) 538
HapII CCGG 1 cut(s) 260
Hin1II CATG 1 cut(s) 197
HinfI GANTC 4 cut(s) 32, 83, 122, 179
HpaII CCGG 1 cut(s) 260
HphI GGTGA 1 cut(s) 16
Hpy166II GTNNAC 5 cut(s) 163, 284, 325, 368, 383
Hpy188I TCNGA 6 cut(s) 75, 127, 141, 295, 442, 465
Hpy188III TCNNGA 1 cut(s) 194
Hpy8I GTNNAC 5 cut(s) 163, 284, 325, 368, 383
HpyAV CCTTC 1 cut(s) 280
HpyCH4III ACNGT 2 cut(s) 406, 502
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 1 cut(s) 482
HpySE526I ACGT 1 cut(s) 14
Hsp92II CATG 1 cut(s) 197
Kzo9I GATC 3 cut(s) 66, 391, 460
LguI GCTCTTC 1 cut(s) 250
LpnPI CCDG 3 cut(s) 168, 273, 293
LweI GCATC 1 cut(s) 66
MaeI CTAG 1 cut(s) 538
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 1 cut(s) 4
MalI GATC 3 cut(s) 68, 393, 462
MboI GATC 3 cut(s) 66, 391, 460
MboII GAAGA 3 cut(s) 237, 460, 500
MflI RGATCY 1 cut(s) 460
MluCI AATT 3 cut(s) 57, 168, 205
MlyI GAGTC 2 cut(s) 131, 188
MmeI TCCRAC 1 cut(s) 105
MnlI CCTC 5 cut(s) 135, 143, 185, 190, 451
MroXI GAANNNNTTC 1 cut(s) 269
MspI CCGG 1 cut(s) 260
MspR9I CCNGG 1 cut(s) 261
Mva1269I GAATGC 1 cut(s) 271
NciI CCSGG 1 cut(s) 261
NdeII GATC 3 cut(s) 66, 391, 460
NlaIII CATG 1 cut(s) 197
NlaIV GGNNCC 1 cut(s) 215
NmuCI GTSAC 1 cut(s) 4
PaeR7I CTCGAG 1 cut(s) 176
PagI TCATGA 1 cut(s) 193
PciSI GCTCTTC 1 cut(s) 250
PctI GAATGC 1 cut(s) 271
PdmI GAANNNNTTC 1 cut(s) 269
PfeI GAWTC 2 cut(s) 32, 83
PleI GAGTC 2 cut(s) 130, 187
PpsI GAGTC 2 cut(s) 130, 187
PspN4I GGNNCC 1 cut(s) 215
PspPI GGNCC 1 cut(s) 214
PspXI VCTCGAGB 1 cut(s) 176
PsuI RGATCY 1 cut(s) 460
RsaI GTAC 4 cut(s) 17, 201, 324, 478
RsaNI GTAC 4 cut(s) 16, 200, 323, 477
SapI GCTCTTC 1 cut(s) 250
Sau3AI GATC 3 cut(s) 66, 391, 460
Sau96I GGNCC 1 cut(s) 214
SchI GAGTC 2 cut(s) 131, 188
ScrFI CCNGG 1 cut(s) 261
SetI ASST 5 cut(s) 17, 177, 201, 245, 352
SfaNI GCATC 1 cut(s) 66
Sfr274I CTCGAG 1 cut(s) 176
SinI GGWCC 1 cut(s) 214
SlaI CTCGAG 1 cut(s) 176
SmlI CTYRAG 2 cut(s) 176, 333
SmoI CTYRAG 2 cut(s) 176, 333
Sse9I AATT 3 cut(s) 57, 168, 205
SspI AATATT 1 cut(s) 225
SspMI CTAG 1 cut(s) 538
StyD4I CCNGG 1 cut(s) 259
TaaI ACNGT 2 cut(s) 406, 502
TaiI ACGT 1 cut(s) 17
TaqI TCGA 2 cut(s) 81, 177
TasI AATT 3 cut(s) 57, 168, 205
TatI WGTACW 2 cut(s) 322, 476
TfiI GAWTC 2 cut(s) 32, 83
TscAI CASTG 1 cut(s) 420
TseFI GTSAC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 4
TspDTI ATGAA 2 cut(s) 182, 279
TspRI CASTG 1 cut(s) 420
VpaK11BI GGWCC 1 cut(s) 214
XapI RAATTY 2 cut(s) 57, 205
XhoI CTCGAG 1 cut(s) 176
XmiI GTMKAC 2 cut(s) 162, 283
XmnI GAANNNNTTC 1 cut(s) 269
XspI CTAG 1 cut(s) 538
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.