Rh5CG453300

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000144
Physical Location & Seq
Forward (+)
63119481 .. 63138250
18770 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG453300.1

Sequence Viewer

Length: 861 bp
ATGGAGTTCATGATTAAAACTGTATTAGTTTGCGGTTGCTTAGTGTCTGGTGGTGTGGTGATTAAGAGACTATTCCGTCACAAGCCCTGCTGGGAGACACTACCTGACGATATCATGGAGGTTATTGTGCCACACCTGAGTGTACGTGATCGTATACGCTTAAGCCTAGTTTGCAAGCCATGGAGCTCAGTTCCTATGTGCAAAGACATCCCTAGTTCTCCTCACAAACTCCCCTGGTTAGTACTCCCTCAACCTGAATCTCAAGCTCTAACTTGCAATGATACCTCCTTAAGCTTTTACGACCTCCATGACGGCAAAGTTGTTAAGTTGGAGCTGCCTGAACCTGTTCGTGGAGCGTGGTTTCGAGGGTCTTCGAAAGGTTGGTTGATTATGATTAAGGAAAGTGAACAAAACACTACAACATTTCTGGTAAATCCAATTTCAGGAGTCCAACACCAACTTCCATCCTTGAGAACGATTCCGTCTTTCCAGAAGTTTGTAGAAACGAAGAAATGGAAACGATATGATTTTGCGCCTGGAAGAAAGGAATGGAATGAAGATTGCTCTATAGGCTTGCTGGGTCGAACTTCCTTCTGCTGGTGGTTCAATTCGGTGGGTTCAATTCACTCTCTCAACTATCTTCTCTCTTCCATCAATGGGTTCAATTCAGTTAGTTTAAATTATGAAATGGAAGAGATAAAATTTATCAGTGGATCTGTAGACAACAAGAAGGGGGATCTAGAGTTGCAACAGGCGATATACTCAACTACCTTCAGGATTGAACGATTGGAATTGTTTGATGAATTTGAAGAATGGCACATGATGCAGGCAAGTCAACTCCTCCTTTCTGTCGTAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

286

Amino Acids

32.91

Weight (kDa)

7.03

Isoelectric Point (pI)

41.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 31 - 63 6.3e-06 F-box-like
Beta-prop_KIB1-4 PF03478 99 - 170 1.7e-10 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 75
AccI GTMKAC 2 cut(s) 154, 720
AciI CCGC 1 cut(s) 33
AclWI GGATC 2 cut(s) 721, 744
AcsI RAATTY 2 cut(s) 701, 803
AcuI CTGAAG 1 cut(s) 757
AfaI GTAC 2 cut(s) 144, 243
AfiI CCNNNNNNNGG 4 cut(s) 350, 443, 597, 657
AflII CTTAAG 2 cut(s) 160, 289
AgsI TTSAA 5 cut(s) 607, 621, 664, 782, 809
AjnI CCWGG 2 cut(s) 233, 535
AjuI GAANNNNNNNTTGG 2 cut(s) 444, 476
AleI CACNNNNGTG 1 cut(s) 138
AluBI AGCT 4 cut(s) 186, 266, 294, 334
AluI AGCT 4 cut(s) 186, 266, 294, 334
Alw21I GWGCWC 1 cut(s) 188
Alw26I GTCTC 2 cut(s) 61, 89
AlwI GGATC 2 cut(s) 721, 744
ApeKI GCWGC 1 cut(s) 334
ApoI RAATTY 2 cut(s) 701, 803
Asp700I GAANNNNTTC 1 cut(s) 345
AspLEI GCGC 1 cut(s) 535
AsuHPI GGTGA 1 cut(s) 70
AsuII TTCGAA 1 cut(s) 374
BanII GRGCYC 1 cut(s) 188
BbsI GAAGAC 1 cut(s) 363
Bbv12I GWGCWC 1 cut(s) 188
BbvI GCAGC 1 cut(s) 321
BccI CCATC 2 cut(s) 472, 659
BceAI ACGGC 1 cut(s) 328
BciT130I CCWGG 2 cut(s) 235, 537
BcoDI GTCTC 2 cut(s) 61, 89
BfaI CTAG 3 cut(s) 167, 213, 740
BfmI CTRYAG 2 cut(s) 567, 717
BfrI CTTAAG 2 cut(s) 160, 289
BisI GCNGC 1 cut(s) 335
BlsI GCNGC 1 cut(s) 336
BmcAI AGTACT 1 cut(s) 243
Bme1390I CCNGG 2 cut(s) 235, 537
BmrFI CCNGG 2 cut(s) 235, 537
BmsI GCATC 1 cut(s) 813
BpiI GAAGAC 1 cut(s) 363
Bpu14I TTCGAA 1 cut(s) 374
BpuEI CTTGAG 2 cut(s) 246, 490
BsaAI YACGTR 1 cut(s) 146
BsaJI CCNNGG 2 cut(s) 179, 233
Bsc4I CCNNNNNNNGG 4 cut(s) 350, 443, 597, 657
Bse3DI GCAATG 1 cut(s) 283
BseBI CCWGG 2 cut(s) 235, 537
BseDI CCNNGG 2 cut(s) 179, 233
BseGI GGATG 2 cut(s) 207, 464
BseLI CCNNNNNNNGG 4 cut(s) 350, 443, 597, 657
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 2 cut(s) 128, 201
BseRI GAGGAG 2 cut(s) 210, 830
BseXI GCAGC 1 cut(s) 321
BseYI CCCAGC 2 cut(s) 90, 577
BsiHKAI GWGCWC 1 cut(s) 188
BslI CCNNNNNNNGG 4 cut(s) 350, 443, 597, 657
BsmAI GTCTC 2 cut(s) 61, 89
Bsp119I TTCGAA 1 cut(s) 374
Bsp1286I GDGCHC 1 cut(s) 188
Bsp143I GATC 3 cut(s) 148, 713, 736
Bsp19I CCATGG 1 cut(s) 179
BspACI CCGC 1 cut(s) 33
BspCNI CTCAG 2 cut(s) 129, 200
BspHI TCATGA 1 cut(s) 9
BspPI GGATC 2 cut(s) 721, 744
BspT104I TTCGAA 1 cut(s) 374
BspTI CTTAAG 2 cut(s) 160, 289
BsrDI GCAATG 1 cut(s) 283
BssECI CCNNGG 2 cut(s) 179, 233
BssMI GATC 3 cut(s) 148, 713, 736
BssNAI GTATAC 1 cut(s) 155
BssT1I CCWWGG 1 cut(s) 179
Bst1107I GTATAC 1 cut(s) 155
Bst2UI CCWGG 2 cut(s) 235, 537
Bst4CI ACNGT 1 cut(s) 22
Bst6I CTCTTC 2 cut(s) 652, 687
BstAFI CTTAAG 2 cut(s) 160, 289
BstAPI GCANNNNNTGC 1 cut(s) 823
BstBAI YACGTR 1 cut(s) 146
BstBI TTCGAA 1 cut(s) 374
BstC8I GCNNGC 3 cut(s) 176, 575, 828
BstDEI CTNAG 3 cut(s) 40, 137, 187
BstDSI CCRYGG 1 cut(s) 179
BstF5I GGATG 2 cut(s) 207, 464
BstHHI GCGC 1 cut(s) 535
BstKTI GATC 3 cut(s) 151, 716, 739
BstMAI GTCTC 2 cut(s) 61, 89
BstMBI GATC 3 cut(s) 148, 713, 736
BstMWI GCNNNNNNNGC 3 cut(s) 171, 570, 823
BstNI CCWGG 2 cut(s) 235, 537
BstSCI CCNGG 2 cut(s) 233, 535
BstSFI CTRYAG 2 cut(s) 567, 717
BstV1I GCAGC 1 cut(s) 321
BstV2I GAAGAC 1 cut(s) 363
BstX2I RGATCY 2 cut(s) 713, 736
BstYI RGATCY 2 cut(s) 713, 736
BstZ17I GTATAC 1 cut(s) 155
BtgI CCRYGG 1 cut(s) 179
BtsCI GGATG 2 cut(s) 207, 464
BtsIMutI CAGTG 1 cut(s) 715
Cac8I GCNNGC 3 cut(s) 176, 575, 828
CciI TCATGA 1 cut(s) 9
CfoI GCGC 1 cut(s) 535
Csp6I GTAC 2 cut(s) 143, 242
CviAII CATG 5 cut(s) 10, 115, 180, 308, 820
CviJI RGCY 8 cut(s) 85, 165, 178, 186, 266, 294, 334, 573
CviKI_1 RGCY 8 cut(s) 85, 165, 178, 186, 266, 294, 334, 573
CviQI GTAC 2 cut(s) 143, 242
DdeI CTNAG 3 cut(s) 40, 137, 187
DpnI GATC 3 cut(s) 150, 715, 738
DpnII GATC 3 cut(s) 148, 713, 736
DraI TTTAAA 1 cut(s) 678
DrdI GACNNNNNNGTC 1 cut(s) 75
DseDI GACNNNNNNGTC 1 cut(s) 75
Eam1104I CTCTTC 2 cut(s) 652, 687
EarI CTCTTC 2 cut(s) 652, 687
Ecl136II GAGCTC 1 cut(s) 186
Eco130I CCWWGG 1 cut(s) 179
Eco24I GRGCYC 1 cut(s) 188
Eco32I GATATC 1 cut(s) 112
Eco53kI GAGCTC 1 cut(s) 186
Eco57I CTGAAG 1 cut(s) 757
EcoICRI GAGCTC 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 233, 535
EcoRV GATATC 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 179
EcoT38I GRGCYC 1 cut(s) 188
ErhI CCWWGG 1 cut(s) 179
FaeI CATG 5 cut(s) 13, 118, 183, 311, 823
FatI CATG 5 cut(s) 9, 114, 179, 307, 819
FblI GTMKAC 2 cut(s) 154, 720
Fnu4HI GCNGC 1 cut(s) 335
FokI GGATG 2 cut(s) 194, 451
FriOI GRGCYC 1 cut(s) 188
Fsp4HI GCNGC 1 cut(s) 335
FspBI CTAG 3 cut(s) 167, 213, 740
GlaI GCGC 1 cut(s) 534
GluI GCNGC 1 cut(s) 335
GsaI CCCAGC 2 cut(s) 94, 581
HhaI GCGC 1 cut(s) 535
Hin1II CATG 5 cut(s) 13, 118, 183, 311, 823
Hin6I GCGC 1 cut(s) 533
HinP1I GCGC 1 cut(s) 533
HincII GTYRAC 1 cut(s) 836
HindII GTYRAC 1 cut(s) 836
HindIII AAGCTT 1 cut(s) 292
HinfI GANTC 3 cut(s) 257, 447, 478
HphI GGTGA 1 cut(s) 70
Hpy166II GTNNAC 5 cut(s) 143, 155, 407, 721, 836
Hpy188III TCNNGA 5 cut(s) 10, 444, 490, 740, 775
Hpy8I GTNNAC 5 cut(s) 143, 155, 407, 721, 836
HpyAV CCTTC 3 cut(s) 601, 724, 781
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 5 cut(s) 174, 201, 276, 748, 826
HpyF10VI GCNNNNNNNGC 3 cut(s) 171, 570, 823
HpyF3I CTNAG 3 cut(s) 40, 137, 187
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 5 cut(s) 13, 118, 183, 311, 823
HspAI GCGC 1 cut(s) 533
Kzo9I GATC 3 cut(s) 148, 713, 736
LmnI GCTCC 3 cut(s) 183, 331, 353
Lsp1109I GCAGC 1 cut(s) 321
LweI GCATC 1 cut(s) 813
MaeI CTAG 3 cut(s) 167, 213, 740
MaeII ACGT 1 cut(s) 145
MaeIII GTNAC 1 cut(s) 77
MalI GATC 3 cut(s) 150, 715, 738
MboI GATC 3 cut(s) 148, 713, 736
MboII GAAGA 8 cut(s) 363, 520, 552, 569, 632, 639, 704, 821
MflI RGATCY 2 cut(s) 713, 736
MhlI GDGCHC 1 cut(s) 188
MluCI AATT 8 cut(s) 438, 607, 621, 664, 679, 701, 791, 803
MlyI GAGTC 1 cut(s) 456
MmeI TCCRAC 2 cut(s) 309, 475
MnlI CCTC 7 cut(s) 112, 231, 258, 295, 314, 359, 851
MroXI GAANNNNTTC 1 cut(s) 345
MseI TTAA 7 cut(s) 15, 63, 161, 290, 324, 396, 677
MslI CAYNNNNRTG 1 cut(s) 138
MspCI CTTAAG 2 cut(s) 160, 289
MspR9I CCNGG 2 cut(s) 235, 537
MvaI CCWGG 2 cut(s) 235, 537
MwoI GCNNNNNNNGC 3 cut(s) 171, 570, 823
NcoI CCATGG 1 cut(s) 179
NdeII GATC 3 cut(s) 148, 713, 736
NlaIII CATG 5 cut(s) 13, 118, 183, 311, 823
NmuCI GTSAC 1 cut(s) 77
NspV TTCGAA 1 cut(s) 374
OliI CACNNNNGTG 1 cut(s) 138
PagI TCATGA 1 cut(s) 9
PdmI GAANNNNTTC 1 cut(s) 345
PfeI GAWTC 2 cut(s) 257, 478
PkrI GCNGC 1 cut(s) 336
PleI GAGTC 1 cut(s) 455
PpsI GAGTC 1 cut(s) 455
Ppu21I YACGTR 1 cut(s) 146
Psp124BI GAGCTC 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 233, 535
PspFI CCCAGC 2 cut(s) 90, 577
PspGI CCWGG 2 cut(s) 233, 535
PsuI RGATCY 2 cut(s) 713, 736
RsaI GTAC 2 cut(s) 144, 243
RsaNI GTAC 2 cut(s) 143, 242
RseI CAYNNNNRTG 1 cut(s) 138
SacI GAGCTC 1 cut(s) 188
SaqAI TTAA 7 cut(s) 15, 63, 161, 290, 324, 396, 677
SatI GCNGC 1 cut(s) 335
Sau3AI GATC 3 cut(s) 148, 713, 736
ScaI AGTACT 1 cut(s) 243
SchI GAGTC 1 cut(s) 456
ScrFI CCNGG 2 cut(s) 235, 537
SduI GDGCHC 1 cut(s) 188
SfaNI GCATC 1 cut(s) 813
SfcI CTRYAG 2 cut(s) 567, 717
SfuI TTCGAA 1 cut(s) 374
SmiMI CAYNNNNRTG 1 cut(s) 138
SmlI CTYRAG 4 cut(s) 160, 261, 289, 469
SmoI CTYRAG 4 cut(s) 160, 261, 289, 469
Sse9I AATT 8 cut(s) 438, 607, 621, 664, 679, 701, 791, 803
SsiI CCGC 1 cut(s) 33
SspMI CTAG 3 cut(s) 167, 213, 740
SstI GAGCTC 1 cut(s) 188
StyD4I CCNGG 2 cut(s) 233, 535
StyI CCWWGG 1 cut(s) 179
TaaI ACNGT 1 cut(s) 22
TaiI ACGT 1 cut(s) 148
TaqI TCGA 3 cut(s) 364, 374, 583
TasI AATT 8 cut(s) 438, 607, 621, 664, 679, 701, 791, 803
TatI WGTACW 1 cut(s) 241
TfiI GAWTC 2 cut(s) 257, 478
Tru1I TTAA 7 cut(s) 15, 63, 161, 290, 324, 396, 677
Tru9I TTAA 7 cut(s) 15, 63, 161, 290, 324, 396, 677
TscAI CASTG 1 cut(s) 715
TseFI GTSAC 1 cut(s) 77
TseI GCWGC 1 cut(s) 334
Tsp45I GTSAC 1 cut(s) 77
TspDTI ATGAA 3 cut(s) 570, 699, 816
TspGWI ACGGA 2 cut(s) 65, 471
TspRI CASTG 1 cut(s) 715
Vha464I CTTAAG 2 cut(s) 160, 289
XapI RAATTY 2 cut(s) 701, 803
XbaI TCTAGA 1 cut(s) 739
XmiI GTMKAC 2 cut(s) 154, 720
XmnI GAANNNNTTC 1 cut(s) 345
XspI CTAG 3 cut(s) 167, 213, 740
ZrmI AGTACT 1 cut(s) 243
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.