RLG00000001327

Protein of unknown function (DUF295)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000144
Physical Location & Seq
Reverse (-)
14140020 .. 14141716
1697 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000001327

Sequence Viewer

Length: 1617 bp
ATGACGAAGATGATTGAGACTCTCTTTGGTTGCTTCAGTGGAAAGCAACGCCAACAAAAGAGAAGCAAGCTTGATCTTGAGAGAAAACTCTTCCGGAAAAAGCTTGAGACGAAGCAAACGGAAGATGAAGGCATGTCTTGGCAGAAACCATCTGCAGATGTCATGGAGAACATTCTTCAGCGTGTCAACGTAGGTGATCGCATACGGTTGAGCATCGTCTCCAAGCCTTGGAACTCAATTGTTACACGAAGAGAAATTCGTGGTGCTCCACATGAACTCCCCTGGTTACTACTCCCTCATCCCACAAAATGGAAGTACTTGAGCTTTCTTAGTCTTTCTGAGGGTAAAGTTGTGAAGTTGAAGCCGCCTAAGCCGGTTCGAGGAGGGTGGATTTATGGGTCTACTAAAGGTTCCTTGATCACAATAAAGGAACAAGGACTGAAATCTACTATGTTTCTAGTAAACCCAATTTCAGGAGCCCTAGACCAACTTCCGCCATTTAAAACAATTCCATCTTTTCGAAAGTATATAAAAACGCGGGAAGAATGGAAACGTTTCGGTGCCAATCTATTCTGCCCTCGCATTGCGGTATCTATGTCGGATAATAATAATTCGAACTGGTGGACGATGGCAGCAGCAATTATAGACTATGAAGATCGTAAGACATTGGGTCATTGGGTGTGTGCAGACCTGGAGACAAGAAATGGAGTGTCTTTCAGTCAAAAGAATGCTGAAGTTGCAGCTACTCGCACTTTAAAGTTTGAAGATGATGGGGTAAAATTGAAATTGAAGTTGGTCTACGACAAGCATGTAAACAAAAATGAGAGTATTGATGAAATGCATAGTGACTATATGGTGGTTTATAATGCAGATTACGGGTCAATGTTGTCTGAATCCACCAACAATGAAGTGTTGATAATCCATCAAATGCTAGATGCATTTTGGAGGATAGAAGATGTTGACGGCAATAATAACAATGGCGTCGGCGACGGCAATGTGATTATTAATGATCAAGACTATGATAATAATGAAGGTAATGTTGACAATAATGGTGGCGGCTTGAATATTGTTGAGGATCAGGAGCATGATGATGAAGCCAATAATCTCAGTGTTGTTGAGGCTGATGAGGAAGACATCTATGAGGAATATGATGATTATGATAATGGCGGCGACCTCAATATTATCGAGGATAATGAGGAAGATATGTATGAGGATCACGATGACAATAATCATGAAGAAGACAATCATCAGGATGAAGAAGCTATTGATGAAGAGGTGCCTCGCTATAATCCATATATGCCGGCAAGGAGTTTTAGAACATACAAGATTGACCCAGATAATGACAACTTTGTCCCGATGCAAAGCTTGGGAAACCAATTGTTATTTTTAGGAGAAGGTGGATCATTTTCTCTTCCAGCTAGTGATTTCGAAGAAAAAGAAAAGAATTGCATTTATTGTGCAACAAATTATGTTCCTCAGTTTCAATTTGCTCCCAAAACATTTATATCTCGTGAGATTGGTGTATTCTACTTCAGTGGTGAAAGAATTGAGCGGTCTTTTCCAAGTCTCGACATGTCAGTGAGGTATCAACCCTCTTGGTTCACTCCATGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

539

Amino Acids

62.0

Weight (kDa)

4.79

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 53 - 85 2.5e-06 F-box domain
Beta-prop_KIB1-4 PF03478 109 - 211 4.9e-07 KIB1-4 beta-propeller
Beta-prop_KIB1-4 PF03478 433 - 509 7e-11 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000144)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g03062 FvH4_2g03731 FvH4_2g04271 FvH4_2g04880 FvH4_2g04887 FvH4_2g06581 FvH4_2g16550 FvH4_2g16551 FvH4_2g16580 FvH4_4g01551 FvH4_4g01581 FvH4_4g01590 FvH4_4g01970 FvH4_4g02080 FvH4_4g02090 FvH4_4g02150 FvH4_5g18721 FvH4_5g33551 FvH4_5g33621 FvH4_7g08620
malus_domestica MD03G1012300.v1.1 MD05G1134000.v1.1 MD17G1018900.v1.1
prunus_persica Prupe.8G151600_v2.0.a1 Prupe.8G152400_v2.0.a1
pyrus_communis pycom03g01090 pycom17g01570
rosa_chinensis RchiOBHm_Chr4g0388141 RchiOBHm_Chr4g0388171 RchiOBHm_Chr4g0393001 RchiOBHm_Chr4g0393391 RchiOBHm_Chr4g0393471 RchiOBHm_Chr5g0063131 RchiOBHm_Chr6g0247891 RchiOBHm_Chr6g0254551 RchiOBHm_Chr6g0254611 RchiOBHm_Chr6g0254631 RchiOBHm_Chr6g0254681 RchiOBHm_Chr6g0280611
rosa_laevigata RLG00000001327 RLG00000001371 RLG00000003555 RLG00000003556 RLG00000009751 RLG00000009755 RLG00000010077 RLG00000012931 RLG00000013040 RLG00000013044 RLG00000013045 RLG00000014594 RLG00000014599 RLG00000014600 RLG00000014604 RLG00000014897 RLG00000014902 RLG00000014904 RLG00000014975 RLG00000014978 RLG00000014979 RLG00000015006 RLG00000023873 RLG00000035608
rosa_multiflora Rmu_co8471587.1_g000001 Rmu_sc0000976.1_g000004 Rmu_sc0001432.1_g000003 Rmu_sc0001432.1_g000004 Rmu_sc0001590.1_g000006 Rmu_sc0003093.1_g000008 Rmu_sc0005045.1_g000035 Rmu_sc0006323.1_g000014 Rmu_sc0007214.1_g000010 Rmu_sc0007839.1_g000011 Rmu_sc0008530.1_g000006 Rmu_sc0008789.1_g000010 Rmu_sc0009578.1_g000013 Rmu_sc0012965.1_g000003 Rmu_sc0036636.1_g000001
rosa_roxburghii Rroxscaffold_178G00437520 Rroxscaffold_178G00437560 Rroxscaffold_2G00106430 Rroxscaffold_5G00334690 Rroxscaffold_5G00338370 Rroxscaffold_5G00356450 Rroxscaffold_6G00388740 Rroxscaffold_6G00406660 Rroxscaffold_7G00187690 Rroxscaffold_7G00187810 Rroxscaffold_7G00187840 Rroxscaffold_7G00187880 Rroxscaffold_7G00205770 Rroxscaffold_7G00205790 Rroxscaffold_7G00205830 Rroxscaffold_7G00205840 Rroxscaffold_7G00205880 Rroxscaffold_7G00205920 Rroxscaffold_7G00205990 Rroxscaffold_7G00210640 Rroxscaffold_7G00210680 Rroxscaffold_7G00211580 Rroxscaffold_7G00211600
rosa_rugosa Rorug03G0315100 Rorug03G0315200 Rorug03G0315800 Rorug04G0001400 Rorug04G0001700 Rorug04G0003000 Rorug05G0354500 Rorug05G0546700 Rorug05G0546800 Rorug05G0546900 Rorug05G0553400 Rorug05G0553500 Rorug05G0553500 Rorug05G0553600 Rorug05G0553900 Rorug05G0554100 Rorug05G0554300 Rorug05G0582500 Rorug06G0132600 Rorug06G0132700 Rorug06G0132800 Rorug06G0133300 Rorug06G0133300 Rorug06G0185400 Rorug07G0074300 Rorug07G0074400 Rorug07G0275100
rosa_samantha Rh3AG195300 Rh3BG225400 Rh3BG225500 Rh3CG220300 Rh3DG220800 Rh3DG220900 Rh4AG020900 Rh4AG021400 Rh4AG047100 Rh4BG015300 Rh4BG041200 Rh4BG042800 Rh4BG043400 Rh4CG021500 Rh4CG021600 Rh4CG050100 Rh4CG050200 Rh5AG414800 Rh5CG453300 Rh6AG062600 Rh6AG070300 Rh6AG070500 Rh6AG070900 Rh6AG071200 Rh6AG099000 Rh6AG245300 Rh6AG245400 Rh6AG245800 Rh6BG055700 Rh6BG056000 Rh6BG062800 Rh6BG063100 Rh6BG063200 Rh6BG063300 Rh6BG247500 Rh6BG247600 Rh6BG247700 Rh6BG248200 Rh6BG249300 Rh6BG508200 Rh6DG034800 Rh6DG053300 Rh6DG053600 Rh6DG053700 Rh6DG059700 Rh6DG059800 Rh6DG082300 Rh6DG240400 Rh7AG202900 Rh7AG203000 Rh7AG407700 Rh7AG429300 Rh7BG202700 Rh7BG403000 Rh7CG213700 Rh7CG448100
rosa_wichuraiana Rw3G017790 Rw4G001400 Rw4G003730 Rw5G039000 Rw6G005550 Rw6G005570 Rw6G006160 Rw6G006180 Rw6G006200 Rw6G006220 Rw6G006250 Rw6G021280

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 864
AasI GACNNNNNNGTC 1 cut(s) 1349
AccB1I GGYRCC 2 cut(s) 560, 1276
AccB7I CCANNNNNTGG 2 cut(s) 228, 309
AccBSI CCGCTC 1 cut(s) 1552
AccI GTMKAC 2 cut(s) 401, 798
AccII CGCG 1 cut(s) 538
AccIII TCCGGA 1 cut(s) 93
AciI CCGC 7 cut(s) 365, 494, 538, 587, 1056, 1167, 1552
AclI AACGTT 1 cut(s) 553
AclWI GGATC 3 cut(s) 1083, 1221, 1408
AcsI RAATTY 1 cut(s) 255
AcuI CTGAAG 4 cut(s) 19, 161, 753, 1516
AcyI GRCGYC 1 cut(s) 981
AfaI GTAC 1 cut(s) 317
AfiI CCNNNNNNNGG 4 cut(s) 228, 309, 380, 473
AflIII ACRYGT 1 cut(s) 1572
AgsI TTSAA 6 cut(s) 361, 764, 784, 790, 1063, 1484
AhdI GACNNNNNGTC 1 cut(s) 669
AjnI CCWGG 2 cut(s) 281, 690
AjuI GAANNNNNNNTTGG 2 cut(s) 776, 808
AluBI AGCT 7 cut(s) 70, 103, 324, 743, 1262, 1365, 1418
AluI AGCT 7 cut(s) 70, 103, 324, 743, 1262, 1365, 1418
Alw21I GWGCWC 1 cut(s) 268
Alw26I GTCTC 5 cut(s) 11, 101, 223, 689, 1571
AlwI GGATC 3 cut(s) 1083, 1221, 1408
Aor13HI TCCGGA 1 cut(s) 93
ApeKI GCWGC 3 cut(s) 632, 635, 740
ApoI RAATTY 1 cut(s) 255
AseI ATTAAT 1 cut(s) 1005
Asp700I GAANNNNTTC 1 cut(s) 554
AsuHPI GGTGA 2 cut(s) 206, 1550
AsuII TTCGAA 3 cut(s) 520, 614, 1428
BanI GGYRCC 2 cut(s) 560, 1276
BanII GRGCYC 1 cut(s) 481
BarI GAAGNNNNNNTAC 2 cut(s) 782, 814
BauI CACGAG 1 cut(s) 1509
BbsI GAAGAC 2 cut(s) 1137, 1245
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 3 cut(s) 644, 647, 752
BccI CCATC 5 cut(s) 157, 520, 622, 764, 930
BceAI ACGGC 2 cut(s) 979, 1006
BciT130I CCWGG 2 cut(s) 283, 692
BclI TGATCA 2 cut(s) 417, 1009
BcoDI GTCTC 5 cut(s) 11, 101, 223, 689, 1571
BfaI CTAG 4 cut(s) 458, 482, 932, 1419
BfmI CTRYAG 1 cut(s) 153
BisI GCNGC 6 cut(s) 365, 633, 636, 741, 1057, 1168
BlsI GCNGC 6 cut(s) 366, 634, 637, 742, 1058, 1169
BmcAI AGTACT 1 cut(s) 317
Bme1390I CCNGG 2 cut(s) 283, 692
BmeRI GACNNNNNGTC 1 cut(s) 669
BmiI GGNNCC 4 cut(s) 412, 478, 562, 1278
BmrFI CCNGG 2 cut(s) 283, 692
BmsI GCATC 3 cut(s) 222, 925, 1347
BpiI GAAGAC 2 cut(s) 1137, 1245
BpmI CTGGAG 1 cut(s) 713
Bpu10I CCTNAGC 1 cut(s) 369
Bpu14I TTCGAA 3 cut(s) 520, 614, 1428
BpuEI CTTGAG 3 cut(s) 98, 125, 340
BsaHI GRCGYC 1 cut(s) 981
BsaJI CCNNGG 2 cut(s) 227, 281
BsaWI WCCGGW 1 cut(s) 93
BsaXI ACNNNNNCTCC 2 cut(s) 261, 291
Bsc4I CCNNNNNNNGG 4 cut(s) 228, 309, 380, 473
Bse118I RCCGGY 2 cut(s) 373, 1300
Bse1I ACTGG 1 cut(s) 623
Bse3DI GCAATG 2 cut(s) 582, 1000
BseAI TCCGGA 1 cut(s) 93
BseBI CCWGG 2 cut(s) 283, 692
BseDI CCNNGG 2 cut(s) 227, 281
BseGI GGATG 2 cut(s) 298, 1258
BseLI CCNNNNNNNGG 4 cut(s) 228, 309, 380, 473
BseMI GCAATG 2 cut(s) 582, 1000
BseMII CTCAG 3 cut(s) 330, 1120, 1490
BseNI ACTGG 1 cut(s) 623
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 3 cut(s) 644, 647, 752
BsgI GTGCAG 1 cut(s) 705
Bsh1236I CGCG 1 cut(s) 538
BshNI GGYRCC 2 cut(s) 560, 1276
BsiHKAI GWGCWC 1 cut(s) 268
BsiSI CCGG 3 cut(s) 94, 374, 1301
BslFI GGGAC 1 cut(s) 1337
BslI CCNNNNNNNGG 4 cut(s) 228, 309, 380, 473
BsmAI GTCTC 5 cut(s) 11, 101, 223, 689, 1571
BsmBI CGTCTC 2 cut(s) 101, 223
BsmFI GGGAC 1 cut(s) 1337
BsmI GAATGC 1 cut(s) 733
Bsp119I TTCGAA 3 cut(s) 520, 614, 1428
Bsp1286I GDGCHC 2 cut(s) 268, 481
Bsp13I TCCGGA 1 cut(s) 93
Bsp143I GATC 8 cut(s) 73, 196, 417, 655, 1009, 1075, 1213, 1400
BspACI CCGC 7 cut(s) 365, 494, 538, 587, 1056, 1167, 1552
BspCNI CTCAG 3 cut(s) 331, 1119, 1489
BspEI TCCGGA 1 cut(s) 93
BspFNI CGCG 1 cut(s) 538
BspHI TCATGA 1 cut(s) 1231
BspLI GGNNCC 4 cut(s) 412, 478, 562, 1278
BspMAI CTGCAG 1 cut(s) 157
BspPI GGATC 3 cut(s) 1083, 1221, 1408
BspT104I TTCGAA 3 cut(s) 520, 614, 1428
BspT107I GGYRCC 2 cut(s) 560, 1276
BsrBI CCGCTC 1 cut(s) 1552
BsrDI GCAATG 2 cut(s) 582, 1000
BsrFI RCCGGY 2 cut(s) 373, 1300
BsrI ACTGG 1 cut(s) 623
BssAI RCCGGY 2 cut(s) 373, 1300
BssECI CCNNGG 2 cut(s) 227, 281
BssMI GATC 8 cut(s) 73, 196, 417, 655, 1009, 1075, 1213, 1400
BssNI GRCGYC 1 cut(s) 981
BssSI CACGAG 1 cut(s) 1509
BssT1I CCWWGG 1 cut(s) 227
Bst2BI CACGAG 1 cut(s) 1509
Bst2UI CCWGG 2 cut(s) 283, 692
Bst4CI ACNGT 1 cut(s) 207
Bst6I CTCTTC 4 cut(s) 95, 244, 1266, 1416
BstACI GRCGYC 1 cut(s) 981
BstBI TTCGAA 3 cut(s) 520, 614, 1428
BstC8I GCNNGC 2 cut(s) 68, 1302
BstDEI CTNAG 5 cut(s) 329, 339, 369, 1106, 1476
BstF5I GGATG 2 cut(s) 298, 1258
BstFNI CGCG 1 cut(s) 538
BstKTI GATC 8 cut(s) 76, 199, 420, 658, 1012, 1078, 1216, 1403
BstMAI GTCTC 5 cut(s) 11, 101, 223, 689, 1571
BstMBI GATC 8 cut(s) 73, 196, 417, 655, 1009, 1075, 1213, 1400
BstMWI GCNNNNNNNGC 2 cut(s) 370, 737
BstNI CCWGG 2 cut(s) 283, 692
BstNSI RCATGY 3 cut(s) 136, 812, 1576
BstSCI CCNGG 2 cut(s) 281, 690
BstSFI CTRYAG 1 cut(s) 153
BstUI CGCG 1 cut(s) 538
BstV1I GCAGC 3 cut(s) 644, 647, 752
BstV2I GAAGAC 2 cut(s) 1137, 1245
BtsCI GGATG 2 cut(s) 298, 1258
BtsIMutI CAGTG 4 cut(s) 43, 1114, 1540, 1584
Cac8I GCNNGC 2 cut(s) 68, 1302
CciI TCATGA 1 cut(s) 1231
Cfr10I RCCGGY 2 cut(s) 373, 1300
CseI GACGC 1 cut(s) 970
Csp6I GTAC 1 cut(s) 316
CspCI CAANNNNNGTGG 2 cut(s) 1033, 1068
CviAII CATG 8 cut(s) 133, 163, 272, 809, 1085, 1232, 1573, 1608
CviQI GTAC 1 cut(s) 316
DdeI CTNAG 5 cut(s) 329, 339, 369, 1106, 1476
DpnI GATC 8 cut(s) 75, 198, 419, 657, 1011, 1077, 1215, 1402
DpnII GATC 8 cut(s) 73, 196, 417, 655, 1009, 1075, 1213, 1400
DraI TTTAAA 2 cut(s) 502, 756
DrdI GACNNNNNNGTC 1 cut(s) 1349
DriI GACNNNNNGTC 1 cut(s) 669
DseDI GACNNNNNNGTC 1 cut(s) 1349
Eam1104I CTCTTC 4 cut(s) 95, 244, 1266, 1416
Eam1105I GACNNNNNGTC 1 cut(s) 669
EarI CTCTTC 4 cut(s) 95, 244, 1266, 1416
EciI GGCGGA 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 227
Eco24I GRGCYC 1 cut(s) 481
Eco57I CTGAAG 4 cut(s) 19, 161, 753, 1516
EcoRII CCWGG 2 cut(s) 281, 690
EcoT14I CCWWGG 1 cut(s) 227
EcoT22I ATGCAT 2 cut(s) 843, 940
EcoT38I GRGCYC 1 cut(s) 481
ErhI CCWWGG 1 cut(s) 227
Esp3I CGTCTC 2 cut(s) 101, 223
FaeI CATG 8 cut(s) 136, 166, 275, 812, 1088, 1235, 1576, 1611
FaqI GGGAC 1 cut(s) 1337
FatI CATG 8 cut(s) 132, 162, 271, 808, 1084, 1231, 1572, 1607
FauI CCCGC 1 cut(s) 531
FbaI TGATCA 2 cut(s) 417, 1009
FblI GTMKAC 2 cut(s) 401, 798
Fnu4HI GCNGC 6 cut(s) 365, 633, 636, 741, 1057, 1168
FokI GGATG 2 cut(s) 285, 1265
FriOI GRGCYC 1 cut(s) 481
Fsp4HI GCNGC 6 cut(s) 365, 633, 636, 741, 1057, 1168
FspBI CTAG 4 cut(s) 458, 482, 932, 1419
GluI GCNGC 6 cut(s) 365, 633, 636, 741, 1057, 1168
GsuI CTGGAG 1 cut(s) 713
HapII CCGG 3 cut(s) 94, 374, 1301
HgaI GACGC 1 cut(s) 970
Hin1I GRCGYC 1 cut(s) 981
Hin1II CATG 8 cut(s) 136, 166, 275, 812, 1088, 1235, 1576, 1611
HincII GTYRAC 3 cut(s) 187, 961, 1042
HindII GTYRAC 3 cut(s) 187, 961, 1042
HindIII AAGCTT 3 cut(s) 68, 101, 1363
HinfI GANTC 2 cut(s) 19, 893
HpaII CCGG 3 cut(s) 94, 374, 1301
HphI GGTGA 2 cut(s) 206, 1550
Hpy166II GTNNAC 9 cut(s) 187, 402, 463, 624, 799, 814, 961, 1042, 1602
Hpy188I TCNGA 3 cut(s) 340, 601, 892
Hpy8I GTNNAC 9 cut(s) 187, 402, 463, 624, 799, 814, 961, 1042, 1602
Hpy99I CGWCG 2 cut(s) 986, 992
HpyAV CCTTC 3 cut(s) 122, 1025, 1388
HpyCH4III ACNGT 1 cut(s) 207
HpyCH4IV ACGT 2 cut(s) 189, 553
HpyCH4V TGCA 9 cut(s) 155, 686, 740, 841, 869, 938, 1360, 1449, 1460
HpyF10VI GCNNNNNNNGC 2 cut(s) 370, 737
HpyF3I CTNAG 5 cut(s) 329, 339, 369, 1106, 1476
HpySE526I ACGT 2 cut(s) 189, 553
Hsp92I GRCGYC 1 cut(s) 981
Hsp92II CATG 8 cut(s) 136, 166, 275, 812, 1088, 1235, 1576, 1611
Kpn2I TCCGGA 1 cut(s) 93
KroI GCCGGC 1 cut(s) 1300
KroNI GCCGGC 1 cut(s) 1302
Ksp22I TGATCA 2 cut(s) 417, 1009
Kzo9I GATC 8 cut(s) 73, 196, 417, 655, 1009, 1075, 1213, 1400
LmnI GCTCC 4 cut(s) 271, 476, 1081, 1495
Lsp1109I GCAGC 3 cut(s) 644, 647, 752
LweI GCATC 3 cut(s) 222, 925, 1347
MaeI CTAG 4 cut(s) 458, 482, 932, 1419
MaeII ACGT 2 cut(s) 189, 553
MaeIII GTNAC 3 cut(s) 241, 285, 845
MalI GATC 8 cut(s) 75, 198, 419, 657, 1011, 1077, 1215, 1402
MbiI CCGCTC 1 cut(s) 1552
MboI GATC 8 cut(s) 73, 196, 417, 655, 1009, 1075, 1213, 1400
MfeI CAATTG 2 cut(s) 237, 1376
MhlI GDGCHC 2 cut(s) 268, 481
MlyI GAGTC 1 cut(s) 13
MmeI TCCRAC 1 cut(s) 579
Mph1103I ATGCAT 2 cut(s) 843, 940
MroI TCCGGA 1 cut(s) 93
MroNI GCCGGC 1 cut(s) 1300
MroXI GAANNNNTTC 1 cut(s) 554
MseI TTAA 3 cut(s) 501, 755, 1005
MslI CAYNNNNRTG 3 cut(s) 1089, 1251, 1577
MspI CCGG 3 cut(s) 94, 374, 1301
MspR9I CCNGG 2 cut(s) 283, 692
MunI CAATTG 2 cut(s) 237, 1376
Mva1269I GAATGC 1 cut(s) 733
MvaI CCWGG 2 cut(s) 283, 692
MvnI CGCG 1 cut(s) 538
MwoI GCNNNNNNNGC 2 cut(s) 370, 737
NaeI GCCGGC 1 cut(s) 1302
NdeII GATC 8 cut(s) 73, 196, 417, 655, 1009, 1075, 1213, 1400
NgoMIV GCCGGC 1 cut(s) 1300
NlaIII CATG 8 cut(s) 136, 166, 275, 812, 1088, 1235, 1576, 1611
NlaIV GGNNCC 4 cut(s) 412, 478, 562, 1278
NmuCI GTSAC 1 cut(s) 845
NsiI ATGCAT 2 cut(s) 843, 940
NspI RCATGY 3 cut(s) 136, 812, 1576
NspV TTCGAA 3 cut(s) 520, 614, 1428
PagI TCATGA 1 cut(s) 1231
PciI ACATGT 1 cut(s) 1572
PctI GAATGC 1 cut(s) 733
PdiI GCCGGC 1 cut(s) 1302
PdmI GAANNNNTTC 1 cut(s) 554
PfeI GAWTC 1 cut(s) 893
PflMI CCANNNNNTGG 2 cut(s) 228, 309
PkrI GCNGC 6 cut(s) 366, 634, 637, 742, 1058, 1169
PleI GAGTC 1 cut(s) 13
PpsI GAGTC 1 cut(s) 13
PscI ACATGT 1 cut(s) 1572
PshBI ATTAAT 1 cut(s) 1005
PsiI TTATAA 1 cut(s) 864
Psp1406I AACGTT 1 cut(s) 553
Psp6I CCWGG 2 cut(s) 281, 690
PspGI CCWGG 2 cut(s) 281, 690
PspN4I GGNNCC 4 cut(s) 412, 478, 562, 1278
PstI CTGCAG 1 cut(s) 157
RsaI GTAC 1 cut(s) 317
RsaNI GTAC 1 cut(s) 316
RseI CAYNNNNRTG 3 cut(s) 1089, 1251, 1577
SaqAI TTAA 3 cut(s) 501, 755, 1005
SatI GCNGC 6 cut(s) 365, 633, 636, 741, 1057, 1168
Sau3AI GATC 8 cut(s) 73, 196, 417, 655, 1009, 1075, 1213, 1400
ScaI AGTACT 1 cut(s) 317
SchI GAGTC 1 cut(s) 13
ScrFI CCNGG 2 cut(s) 283, 692
SduI GDGCHC 2 cut(s) 268, 481
SfaNI GCATC 3 cut(s) 222, 925, 1347
SfcI CTRYAG 1 cut(s) 153
SfuI TTCGAA 3 cut(s) 520, 614, 1428
SmiMI CAYNNNNRTG 3 cut(s) 1089, 1251, 1577
SmlI CTYRAG 3 cut(s) 77, 104, 319
SmoI CTYRAG 3 cut(s) 77, 104, 319
SsiI CCGC 7 cut(s) 365, 494, 538, 587, 1056, 1167, 1552
SspI AATATT 2 cut(s) 1066, 1180
SspMI CTAG 4 cut(s) 458, 482, 932, 1419
StyD4I CCNGG 2 cut(s) 281, 690
StyI CCWWGG 1 cut(s) 227
TaaI ACNGT 1 cut(s) 207
TaiI ACGT 2 cut(s) 192, 556
TaqI TCGA 6 cut(s) 379, 520, 614, 1185, 1428, 1569
TatI WGTACW 1 cut(s) 315
TauI GCSGC 3 cut(s) 367, 1059, 1170
TfiI GAWTC 1 cut(s) 893
Tru1I TTAA 3 cut(s) 501, 755, 1005
Tru9I TTAA 3 cut(s) 501, 755, 1005
TscAI CASTG 4 cut(s) 43, 1114, 1540, 1584
TseFI GTSAC 1 cut(s) 845
TseI GCWGC 3 cut(s) 632, 635, 740
Tsp45I GTSAC 1 cut(s) 845
TspGWI ACGGA 1 cut(s) 134
TspRI CASTG 4 cut(s) 43, 1114, 1540, 1584
Van91I CCANNNNNTGG 2 cut(s) 228, 309
VspI ATTAAT 1 cut(s) 1005
XapI RAATTY 1 cut(s) 255
XceI RCATGY 3 cut(s) 136, 812, 1576
XmiI GTMKAC 2 cut(s) 401, 798
XmnI GAANNNNTTC 1 cut(s) 554
XspI CTAG 4 cut(s) 458, 482, 932, 1419
ZrmI AGTACT 1 cut(s) 317
Zsp2I ATGCAT 2 cut(s) 843, 940
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.