Rmu_sc0004049.1_g000006

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004049.1
OG0000508
Physical Location & Seq
Forward (+)
19864 .. 21413
1550 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004049.1_g000006.1.cds

Sequence Viewer

Length: 1098 bp
atggtgttgaaaaagcgaagagcatcaattccattacggatatctattcctcctcttacttctgtatggcggcgtgatgtcttcctaagttgcgaccatgaagacattttctccgaccacttgtaccacaaattgaagtatcaagaaatttgtgctttccgggatggccaacacttgcaaagaaacacaagtcctcttgacctctttggtgcaatcgaagaatcgaaatttgccgttgttgttctctcgcaaaactatgcttcttcgccccagcgcttgaatgaactttcaaaaattctagagtgcatgaaagataggaacaggattctgccggtgtttcgtgatgttaatctgttccatgtccaaaaacaaaaaggggcttttgagaaagcatttgaaaagcatgaagaaaggtttcaggatgacttggaaaatgttcaagcctggagagatgctttaaccaaagtgtgcaattttgctggatggactaccaatgataggaatgaagtacaggttatagaagaaattactgaagcactgtggaacaaactacattctagactcagtccaacggaaaaactgcacccgacgtccacacacaacttggtcgcattcactagcattagtgttaatgaggaccaccgtgaccataaccagccaataaaaattacagatgtgacggctacttcagtagagtggaagtttttgattactaaccttggcctagagattttgtcagctgcttttgatcaggcttcctcgtcaagtaagccacactatgcactatttggtatgctgttttctattgcagccttgttcatttgcatttgggagctcatttacaagggcataaagaatagagtagtattgaagaagttcggaatgctctggtggttttattatccacatccccacaatagcatgccttttggtactgtccctgaaattttcggattaattggaagcatcgctcagtgcatatgctctataactcaatacatttacctctctcggcatgctgataatcccataaaactatcacttttaccagcaatctttcttttgtgtttggctggttcaagactaaataggaattaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

365

Amino Acids

42.13

Weight (kDa)

8.53

Isoelectric Point (pI)

49.48

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 593
AciI CCGC 1 cut(s) 70
AcoI YGGCCR 1 cut(s) 166
AcsI RAATTY 4 cut(s) 147, 227, 294, 945
AcuI CTGAAG 2 cut(s) 552, 672
AcyI GRCGYC 1 cut(s) 590
AfaI GTAC 3 cut(s) 125, 510, 934
AfeI AGCGCT 1 cut(s) 275
AfiI CCNNNNNNNGG 1 cut(s) 498
AgsI TTSAA 8 cut(s) 10, 136, 280, 291, 398, 440, 871, 1080
AjnI CCWGG 1 cut(s) 443
AluBI AGCT 2 cut(s) 740, 835
AluI AGCT 2 cut(s) 740, 835
Alw21I GWGCWC 1 cut(s) 837
Aor51HI AGCGCT 1 cut(s) 275
AoxI GGCC 2 cut(s) 166, 721
ApeKI GCWGC 2 cut(s) 740, 809
ApoI RAATTY 4 cut(s) 147, 227, 294, 945
AseI ATTAAT 1 cut(s) 956
Asp700I GAANNNNTTC 3 cut(s) 414, 435, 875
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 637
AsuC2I CCSGG 1 cut(s) 161
AvaII GGWCC 1 cut(s) 637
BalI TGGCCA 1 cut(s) 168
BanII GRGCYC 1 cut(s) 837
BbsI GAAGAC 2 cut(s) 73, 108
Bbv12I GWGCWC 1 cut(s) 837
BbvI GCAGC 2 cut(s) 727, 821
BccI CCATC 2 cut(s) 158, 477
BceAI ACGGC 2 cut(s) 218, 696
BciT130I CCWGG 1 cut(s) 445
BclI TGATCA 1 cut(s) 748
BcnI CCSGG 1 cut(s) 161
BfaI CTAG 4 cut(s) 299, 558, 618, 725
BfoI RGCGCY 1 cut(s) 277
BisI GCNGC 3 cut(s) 71, 741, 810
BlsI GCNGC 3 cut(s) 72, 742, 811
Bme1390I CCNGG 2 cut(s) 161, 445
Bme18I GGWCC 1 cut(s) 637
BmgT120I GGNCC 1 cut(s) 637
BmrFI CCNGG 2 cut(s) 161, 445
BmsI GCATC 3 cut(s) 32, 442, 975
BpiI GAAGAC 2 cut(s) 73, 108
BpmI CTGGAG 1 cut(s) 466
BpuMI CCSGG 1 cut(s) 161
BsaBI GATNNNNATC 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 590
BsaJI CCNNGG 1 cut(s) 718
BsaXI ACNNNNNCTCC 2 cut(s) 95, 125
Bsc4I CCNNNNNNNGG 1 cut(s) 498
Bse118I RCCGGY 1 cut(s) 331
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 1 cut(s) 445
BseDI CCNNGG 1 cut(s) 718
BseGI GGATG 4 cut(s) 169, 427, 488, 907
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 1 cut(s) 498
BseMII CTCAG 2 cut(s) 577, 986
BseRI GAGGAG 1 cut(s) 42
BseXI GCAGC 2 cut(s) 727, 821
BseYI CCCAGC 1 cut(s) 270
BsgI GTGCAG 1 cut(s) 566
BshFI GGCC 2 cut(s) 168, 723
BsiHKAI GWGCWC 1 cut(s) 837
BsiSI CCGG 2 cut(s) 160, 332
BslFI GGGAC 1 cut(s) 923
BslI CCNNNNNNNGG 1 cut(s) 498
BsmFI GGGAC 1 cut(s) 923
BsmI GAATGC 2 cut(s) 611, 888
BsnI GGCC 2 cut(s) 168, 723
Bsp1286I GDGCHC 1 cut(s) 837
Bsp143I GATC 1 cut(s) 748
BspACI CCGC 1 cut(s) 70
BspANI GGCC 2 cut(s) 168, 723
BspCNI CTCAG 2 cut(s) 576, 985
BspQI GCTCTTC 1 cut(s) 13
BsrFI RCCGGY 1 cut(s) 331
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 1 cut(s) 718
BssMI GATC 1 cut(s) 748
BssNI GRCGYC 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 718
Bst2UI CCWGG 1 cut(s) 445
Bst4CI ACNGT 3 cut(s) 540, 644, 937
Bst6I CTCTTC 1 cut(s) 13
BstACI GRCGYC 1 cut(s) 590
BstC8I GCNNGC 2 cut(s) 923, 1017
BstDEI CTNAG 3 cut(s) 86, 563, 972
BstF5I GGATG 4 cut(s) 169, 427, 488, 907
BstH2I RGCGCY 1 cut(s) 277
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 1 cut(s) 751
BstMBI GATC 1 cut(s) 748
BstNI CCWGG 1 cut(s) 445
BstNSI RCATGY 2 cut(s) 925, 1019
BstSCI CCNGG 2 cut(s) 159, 443
BstV1I GCAGC 2 cut(s) 727, 821
BstV2I GAAGAC 2 cut(s) 73, 108
BsuRI GGCC 2 cut(s) 168, 723
BtgZI GCGATG 1 cut(s) 952
BtsCI GGATG 4 cut(s) 169, 427, 488, 907
BtsIMutI CAGTG 2 cut(s) 536, 980
Cac8I GCNNGC 2 cut(s) 923, 1017
CfoI GCGC 1 cut(s) 276
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 637
Csp6I GTAC 3 cut(s) 124, 509, 933
CviAII CATG 6 cut(s) 98, 307, 359, 404, 922, 1016
CviQI GTAC 3 cut(s) 124, 509, 933
DdeI CTNAG 3 cut(s) 86, 563, 972
DpnI GATC 1 cut(s) 750
DpnII GATC 1 cut(s) 748
EaeI YGGCCR 1 cut(s) 166
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 835
Eco130I CCWWGG 1 cut(s) 718
Eco24I GRGCYC 1 cut(s) 837
Eco32I GATATC 1 cut(s) 42
Eco47I GGWCC 1 cut(s) 637
Eco47III AGCGCT 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 2 cut(s) 552, 672
EcoICRI GAGCTC 1 cut(s) 835
EcoRII CCWGG 1 cut(s) 443
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 718
EcoT38I GRGCYC 1 cut(s) 837
ErhI CCWWGG 1 cut(s) 718
FaeI CATG 6 cut(s) 101, 310, 362, 407, 925, 1019
FaqI GGGAC 1 cut(s) 923
FatI CATG 6 cut(s) 97, 306, 358, 403, 921, 1015
FauNDI CATATG 1 cut(s) 980
FbaI TGATCA 1 cut(s) 748
Fnu4HI GCNGC 3 cut(s) 71, 741, 810
FokI GGATG 4 cut(s) 176, 434, 495, 894
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 3 cut(s) 71, 741, 810
FspBI CTAG 4 cut(s) 299, 558, 618, 725
GlaI GCGC 1 cut(s) 275
GluI GCNGC 3 cut(s) 71, 741, 810
GsaI CCCAGC 1 cut(s) 274
GsuI CTGGAG 1 cut(s) 466
HaeII RGCGCY 1 cut(s) 277
HaeIII GGCC 2 cut(s) 168, 723
HapII CCGG 2 cut(s) 160, 332
HhaI GCGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 590
Hin1II CATG 6 cut(s) 101, 310, 362, 407, 925, 1019
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HinfI GANTC 3 cut(s) 221, 325, 561
HpaII CCGG 2 cut(s) 160, 332
Hpy166II GTNNAC 1 cut(s) 594
Hpy188I TCNGA 3 cut(s) 115, 881, 953
Hpy188III TCNNGA 7 cut(s) 143, 197, 299, 341, 419, 558, 1080
Hpy8I GTNNAC 1 cut(s) 594
Hpy99I CGWCG 1 cut(s) 592
HpyCH4III ACNGT 3 cut(s) 540, 644, 937
HpyCH4IV ACGT 1 cut(s) 590
HpyCH4V TGCA 9 cut(s) 178, 212, 306, 471, 583, 782, 809, 825, 978
HpyF3I CTNAG 3 cut(s) 86, 563, 972
HpySE526I ACGT 1 cut(s) 590
Hsp92I GRCGYC 1 cut(s) 590
Hsp92II CATG 6 cut(s) 101, 310, 362, 407, 925, 1019
HspAI GCGC 1 cut(s) 274
Ksp22I TGATCA 1 cut(s) 748
Kzo9I GATC 1 cut(s) 748
LguI GCTCTTC 1 cut(s) 13
LmnI GCTCC 1 cut(s) 832
Lsp1109I GCAGC 2 cut(s) 727, 821
LweI GCATC 3 cut(s) 32, 442, 975
MaeI CTAG 4 cut(s) 299, 558, 618, 725
MaeII ACGT 1 cut(s) 590
MaeIII GTNAC 2 cut(s) 644, 676
MalI GATC 1 cut(s) 750
MboI GATC 1 cut(s) 748
MboII GAAGA 8 cut(s) 30, 73, 113, 230, 255, 419, 533, 883
MhlI GDGCHC 1 cut(s) 837
MlsI TGGCCA 1 cut(s) 168
MluNI TGGCCA 1 cut(s) 168
MlyI GAGTC 1 cut(s) 555
MmeI TCCRAC 2 cut(s) 138, 593
MnlI CCTC 7 cut(s) 60, 63, 204, 212, 628, 769, 1016
Mox20I TGGCCA 1 cut(s) 168
MroXI GAANNNNTTC 3 cut(s) 414, 435, 875
MscI TGGCCA 1 cut(s) 168
MseI TTAA 5 cut(s) 348, 458, 630, 956, 1096
Msp20I TGGCCA 1 cut(s) 168
MspA1I CMGCKG 1 cut(s) 740
MspI CCGG 2 cut(s) 160, 332
MspR9I CCNGG 2 cut(s) 161, 445
Mva1269I GAATGC 2 cut(s) 611, 888
MvaI CCWGG 1 cut(s) 445
NciI CCSGG 1 cut(s) 161
NdeI CATATG 1 cut(s) 980
NdeII GATC 1 cut(s) 748
NlaIII CATG 6 cut(s) 101, 310, 362, 407, 925, 1019
NmeAIII GCCGAG 1 cut(s) 991
NmuCI GTSAC 2 cut(s) 644, 676
NspI RCATGY 2 cut(s) 925, 1019
PaeI GCATGC 2 cut(s) 925, 1019
PciSI GCTCTTC 1 cut(s) 13
PctI GAATGC 2 cut(s) 611, 888
PdmI GAANNNNTTC 3 cut(s) 414, 435, 875
PfeI GAWTC 2 cut(s) 221, 325
PflFI GACNNNGTC 1 cut(s) 564
PfoI TCCNGGA 1 cut(s) 159
PkrI GCNGC 3 cut(s) 72, 742, 811
PleI GAGTC 1 cut(s) 555
PpsI GAGTC 1 cut(s) 555
PshBI ATTAAT 1 cut(s) 956
Psp124BI GAGCTC 1 cut(s) 837
Psp6I CCWGG 1 cut(s) 443
PspFI CCCAGC 1 cut(s) 270
PspGI CCWGG 1 cut(s) 443
PspPI GGNCC 1 cut(s) 637
PsyI GACNNNGTC 1 cut(s) 564
PvuII CAGCTG 1 cut(s) 740
RsaI GTAC 3 cut(s) 125, 510, 934
RsaNI GTAC 3 cut(s) 124, 509, 933
SacI GAGCTC 1 cut(s) 837
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 5 cut(s) 348, 458, 630, 956, 1096
SatI GCNGC 3 cut(s) 71, 741, 810
Sau3AI GATC 1 cut(s) 748
Sau96I GGNCC 1 cut(s) 637
SchI GAGTC 1 cut(s) 555
ScrFI CCNGG 2 cut(s) 161, 445
SduI GDGCHC 1 cut(s) 837
SetI ASST 8 cut(s) 204, 416, 516, 593, 720, 742, 837, 1008
SfaNI GCATC 3 cut(s) 32, 442, 975
SinI GGWCC 1 cut(s) 637
SphI GCATGC 2 cut(s) 925, 1019
SsiI CCGC 1 cut(s) 70
SspMI CTAG 4 cut(s) 299, 558, 618, 725
SstI GAGCTC 1 cut(s) 837
StyD4I CCNGG 2 cut(s) 159, 443
StyI CCWWGG 1 cut(s) 718
TaaI ACNGT 3 cut(s) 540, 644, 937
TaiI ACGT 1 cut(s) 593
TaqI TCGA 2 cut(s) 216, 224
TatI WGTACW 1 cut(s) 508
TauI GCSGC 1 cut(s) 73
TfiI GAWTC 2 cut(s) 221, 325
Tru1I TTAA 5 cut(s) 348, 458, 630, 956, 1096
Tru9I TTAA 5 cut(s) 348, 458, 630, 956, 1096
TscAI CASTG 2 cut(s) 543, 980
TseFI GTSAC 2 cut(s) 644, 676
TseI GCWGC 2 cut(s) 740, 809
Tsp45I GTSAC 2 cut(s) 644, 676
TspDTI ATGAA 6 cut(s) 114, 297, 323, 420, 519, 808
TspGWI ACGGA 2 cut(s) 52, 587
TspRI CASTG 2 cut(s) 543, 980
Tth111I GACNNNGTC 1 cut(s) 564
VpaK11BI GGWCC 1 cut(s) 637
VspI ATTAAT 1 cut(s) 956
XapI RAATTY 4 cut(s) 147, 227, 294, 945
XbaI TCTAGA 2 cut(s) 298, 557
XceI RCATGY 2 cut(s) 925, 1019
XcmI CCANNNNNNNNNTGG 1 cut(s) 601
XmnI GAANNNNTTC 3 cut(s) 414, 435, 875
XspI CTAG 4 cut(s) 299, 558, 618, 725
ZraI GACGTC 1 cut(s) 591
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.