Rroxscaffold_7G00205750

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000508
Physical Location & Seq
Forward (+)
55384369 .. 55385752
1384 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00205750.1

Sequence Viewer

Length: 777 bp
ATGGAGAACCAAGAAGCTTGTGTGATTGAGATCCGCCCTTCTTCTTTTTCTTCAAAGAGGGCTTTGGAGAATTGGAGGAAGCTATCTGCTGCCCTCAGGAACCCCAACAAGCGACAGTTTCTCTTCAACCCTGCGGCTCGCTCCCTTTGGATTGAGAAGCAGGAGAAACACATCCAGACCGCATTGCAATTAGTGAATGCTCGCCGGGATCAGTTGTCTAACAGCAAGTTACCTTTGCTTGGCAGTAGTTCCGGTGACGAGTCAAGGCACACAACAAAGGCTACATCAACAGTATGGGTCTTTTTACTCACCAACCTCGGCCTAGAGACATTATCGGCCGTCTTTGATCAAATTGCCTCTCCAAGCAAGCCTGACTATGCACTATTCGGAATGGTGTTGGCGATTTTAGCTGTGCTCATTTGCATCTTGGAGCTCATTCACAAGGGCATAAAGGAAGGAGTTGAGCTGAAAAGATGGGGAAAGTTGTGCTGGTTTTATTATCCACCTCCTAATGAATCACTTTTTGGTACTTTCCCAGATATTTGTGGATTAGTCATTGCCATTTCACAGTATATTTGCTCCACTGTTCAGTATGTTTATCTGTGCAGGAATGCTAATAACCCCATCAAATTATCCGTTTTGCCGGCCATCTTTCTTATTTGTTTGGCTGGTTCAAAACTAATTAGGAACAGAAGCTACACCACAGATGAGACCTTCAAACATACTACTCATCATATTGCAAAGATTAATCTTTGTAGATTTTGGCATTCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

258

Amino Acids

29.24

Weight (kDa)

9.37

Isoelectric Point (pI)

54.77

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 34, 134, 180
AclWI GGATC 2 cut(s) 25, 216
AcoI YGGCCR 2 cut(s) 336, 645
AfaI GTAC 1 cut(s) 529
AfiI CCNNNNNNNGG 1 cut(s) 239
AgsI TTSAA 4 cut(s) 54, 127, 675, 718
AjuI GAANNNNNNNTTGG 2 cut(s) 507, 539
AluBI AGCT 6 cut(s) 17, 82, 410, 433, 466, 696
AluI AGCT 6 cut(s) 17, 82, 410, 433, 466, 696
Alw21I GWGCWC 2 cut(s) 417, 435
Alw26I GTCTC 2 cut(s) 320, 704
AlwI GGATC 2 cut(s) 25, 216
AoxI GGCC 3 cut(s) 319, 336, 645
ApeKI GCWGC 1 cut(s) 89
AseI ATTAAT 1 cut(s) 747
AsuC2I CCSGG 1 cut(s) 206
AsuHPI GGTGA 2 cut(s) 266, 301
AxyI CCTNAGG 1 cut(s) 95
BanII GRGCYC 1 cut(s) 435
Bbv12I GWGCWC 2 cut(s) 417, 435
BbvI GCAGC 1 cut(s) 76
BccI CCATC 3 cut(s) 468, 632, 656
BceAI ACGGC 1 cut(s) 323
BclI TGATCA 1 cut(s) 346
BcnI CCSGG 1 cut(s) 206
BcoDI GTCTC 2 cut(s) 320, 704
BfaI CTAG 1 cut(s) 323
BisI GCNGC 2 cut(s) 90, 135
BlsI GCNGC 2 cut(s) 91, 136
Bme1390I CCNGG 1 cut(s) 206
BmiI GGNNCC 1 cut(s) 101
BmrFI CCNGG 1 cut(s) 206
BmsI GCATC 1 cut(s) 432
BpuMI CCSGG 1 cut(s) 206
BsaBI GATNNNNATC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 704
BsaJI CCNNGG 1 cut(s) 316
BsaWI WCCGGW 1 cut(s) 251
Bsc4I CCNNNNNNNGG 1 cut(s) 239
Bse118I RCCGGY 1 cut(s) 643
Bse21I CCTNAGG 1 cut(s) 95
Bse3DI GCAATG 2 cut(s) 182, 555
Bse8I GATNNNNATC 1 cut(s) 29
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 1 cut(s) 171
BseJI GATNNNNATC 1 cut(s) 29
BseLI CCNNNNNNNGG 1 cut(s) 239
BseMI GCAATG 2 cut(s) 182, 555
BseMII CTCAG 1 cut(s) 109
BseX3I CGGCCG 1 cut(s) 336
BseXI GCAGC 1 cut(s) 76
BsgI GTGCAG 1 cut(s) 625
Bsh1285I CGRYCG 1 cut(s) 339
BshFI GGCC 3 cut(s) 321, 338, 647
BsiEI CGRYCG 1 cut(s) 339
BsiHKAI GWGCWC 2 cut(s) 417, 435
BsiSI CCGG 3 cut(s) 205, 252, 644
BslI CCNNNNNNNGG 1 cut(s) 239
BsmAI GTCTC 2 cut(s) 320, 704
BsmI GAATGC 3 cut(s) 202, 616, 766
BsnI GGCC 3 cut(s) 321, 338, 647
Bso31I GGTCTC 1 cut(s) 704
Bsp1286I GDGCHC 2 cut(s) 417, 435
Bsp143I GATC 3 cut(s) 30, 208, 346
BspACI CCGC 3 cut(s) 34, 134, 180
BspANI GGCC 3 cut(s) 321, 338, 647
BspCNI CTCAG 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 101
BspPI GGATC 2 cut(s) 25, 216
BspTNI GGTCTC 1 cut(s) 704
BsrDI GCAATG 2 cut(s) 182, 555
BsrFI RCCGGY 1 cut(s) 643
BssAI RCCGGY 1 cut(s) 643
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 3 cut(s) 30, 208, 346
Bst4CI ACNGT 4 cut(s) 117, 292, 570, 586
Bst6I CTCTTC 1 cut(s) 128
BstC8I GCNNGC 4 cut(s) 139, 202, 368, 645
BstDEI CTNAG 1 cut(s) 95
BstF5I GGATG 1 cut(s) 171
BstKTI GATC 3 cut(s) 33, 211, 349
BstMAI GTCTC 2 cut(s) 320, 704
BstMBI GATC 3 cut(s) 30, 208, 346
BstMCI CGRYCG 1 cut(s) 339
BstMWI GCNNNNNNNGC 1 cut(s) 407
BstSCI CCNGG 1 cut(s) 204
BstV1I GCAGC 1 cut(s) 76
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BstZI CGGCCG 1 cut(s) 336
Bsu36I CCTNAGG 1 cut(s) 95
BsuRI GGCC 3 cut(s) 321, 338, 647
BtsCI GGATG 1 cut(s) 171
BtsIMutI CAGTG 1 cut(s) 582
Cac8I GCNNGC 4 cut(s) 139, 202, 368, 645
Cfr10I RCCGGY 1 cut(s) 643
Csp6I GTAC 1 cut(s) 528
CviQI GTAC 1 cut(s) 528
DdeI CTNAG 1 cut(s) 95
DpnI GATC 3 cut(s) 32, 210, 348
DpnII GATC 3 cut(s) 30, 208, 346
EaeI YGGCCR 2 cut(s) 336, 645
EagI CGGCCG 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 128
EarI CTCTTC 1 cut(s) 128
EciI GGCGGA 1 cut(s) 23
Ecl136II GAGCTC 1 cut(s) 433
EclXI CGGCCG 1 cut(s) 336
Eco24I GRGCYC 1 cut(s) 435
Eco31I GGTCTC 1 cut(s) 704
Eco52I CGGCCG 1 cut(s) 336
Eco53kI GAGCTC 1 cut(s) 433
Eco81I CCTNAGG 1 cut(s) 95
EcoICRI GAGCTC 1 cut(s) 433
EcoT38I GRGCYC 1 cut(s) 435
FaiI YATR 7 cut(s) 295, 378, 449, 573, 594, 723, 735
FbaI TGATCA 1 cut(s) 346
Fnu4HI GCNGC 2 cut(s) 90, 135
FokI GGATG 1 cut(s) 158
FriOI GRGCYC 1 cut(s) 435
Fsp4HI GCNGC 2 cut(s) 90, 135
FspBI CTAG 1 cut(s) 323
GluI GCNGC 2 cut(s) 90, 135
HaeIII GGCC 3 cut(s) 321, 338, 647
HapII CCGG 3 cut(s) 205, 252, 644
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 2 cut(s) 260, 515
HpaII CCGG 3 cut(s) 205, 252, 644
HphI GGTGA 2 cut(s) 266, 301
Hpy188I TCNGA 1 cut(s) 389
Hpy188III TCNNGA 2 cut(s) 97, 175
HpyAV CCTTC 3 cut(s) 48, 449, 724
HpyCH4III ACNGT 4 cut(s) 117, 292, 570, 586
HpyCH4V TGCA 5 cut(s) 187, 380, 423, 606, 740
HpyF10VI GCNNNNNNNGC 1 cut(s) 407
HpyF3I CTNAG 1 cut(s) 95
KroI GCCGGC 1 cut(s) 643
KroNI GCCGGC 1 cut(s) 645
Ksp22I TGATCA 1 cut(s) 346
Kzo9I GATC 3 cut(s) 30, 208, 346
LmnI GCTCC 3 cut(s) 146, 430, 584
Lsp1109I GCAGC 1 cut(s) 76
LweI GCATC 1 cut(s) 432
MaeI CTAG 1 cut(s) 323
MaeIII GTNAC 2 cut(s) 228, 254
MalI GATC 3 cut(s) 32, 210, 348
MboI GATC 3 cut(s) 30, 208, 346
MboII GAAGA 3 cut(s) 33, 42, 115
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 2 cut(s) 417, 435
MluCI AATT 5 cut(s) 70, 188, 351, 629, 681
MlyI GAGTC 1 cut(s) 269
MnlI CCTC 6 cut(s) 51, 69, 104, 326, 367, 516
MroNI GCCGGC 1 cut(s) 643
MseI TTAA 1 cut(s) 747
MspI CCGG 3 cut(s) 205, 252, 644
MspR9I CCNGG 1 cut(s) 206
Mva1269I GAATGC 3 cut(s) 202, 616, 766
MwoI GCNNNNNNNGC 1 cut(s) 407
NaeI GCCGGC 1 cut(s) 645
NciI CCSGG 1 cut(s) 206
NdeII GATC 3 cut(s) 30, 208, 346
NgoMIV GCCGGC 1 cut(s) 643
NlaIV GGNNCC 1 cut(s) 101
NmeAIII GCCGAG 1 cut(s) 297
NmuCI GTSAC 1 cut(s) 254
PctI GAATGC 3 cut(s) 202, 616, 766
PdiI GCCGGC 1 cut(s) 645
PfeI GAWTC 1 cut(s) 515
PkrI GCNGC 2 cut(s) 91, 136
PleI GAGTC 1 cut(s) 268
PpsI GAGTC 1 cut(s) 268
PshBI ATTAAT 1 cut(s) 747
Psp124BI GAGCTC 1 cut(s) 435
PspN4I GGNNCC 1 cut(s) 101
PsrI GAACNNNNNNTAC 2 cut(s) 680, 712
PsuI RGATCY 1 cut(s) 30
RsaI GTAC 1 cut(s) 529
RsaNI GTAC 1 cut(s) 528
SacI GAGCTC 1 cut(s) 435
SaqAI TTAA 1 cut(s) 747
SatI GCNGC 2 cut(s) 90, 135
Sau3AI GATC 3 cut(s) 30, 208, 346
SchI GAGTC 1 cut(s) 269
ScrFI CCNGG 1 cut(s) 206
SduI GDGCHC 2 cut(s) 417, 435
SfaNI GCATC 1 cut(s) 432
Sse9I AATT 5 cut(s) 70, 188, 351, 629, 681
SsiI CCGC 3 cut(s) 34, 134, 180
SspMI CTAG 1 cut(s) 323
SstI GAGCTC 1 cut(s) 435
StyD4I CCNGG 1 cut(s) 204
TaaI ACNGT 4 cut(s) 117, 292, 570, 586
TasI AATT 5 cut(s) 70, 188, 351, 629, 681
TauI GCSGC 1 cut(s) 137
TfiI GAWTC 1 cut(s) 515
Tru1I TTAA 1 cut(s) 747
Tru9I TTAA 1 cut(s) 747
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 1 cut(s) 254
TseI GCWGC 1 cut(s) 89
Tsp45I GTSAC 1 cut(s) 254
TspDTI ATGAA 1 cut(s) 528
TspGWI ACGGA 1 cut(s) 625
TspRI CASTG 1 cut(s) 589
VspI ATTAAT 1 cut(s) 747
XspI CTAG 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.