pycom05g07020

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0000508
Physical Location & Seq
Reverse (-)
9950425 .. 9951759
1335 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g07020.2

Sequence Viewer

Length: 660 bp
ATGGATTTTGAGCTTCAAGGAAAACCACATTCCAAAGGATCTGCAGGCTCTAGCGGATCGCATGTGATAAACATTCATGACGAGCCCAGGCACACCAGCTTTCTTAGCTTATCTAATTCTGTGGATTACGGTAGTTATTACAAGCTAAGGCCACCGGAGGAGCCCAAGTACATTAGCAAGGACATTATATCCAGTTTGCAGAAACAAGTCGATGATAATGGCAAGCCAAGGCAGGACACAAAGTCTACAAATTCAGAGAAGTGGATCTTGTTCTTCATCGCTAACCTTGGCATAGAGACCGCTTCAGCTGTTTTTGATCAGCTTTCCTCTTTAAATCATGCCCACTTTGCACTCATTGGTATGCTGCTGGCTATGCTAGCCGTTCTCATTTGCATTTTGGAGCTGATTCTCAAAGGTAAAGAGGAAAGAGTTGAGTTAAGGGCGTGGGGAATGATATGGTGGTTATATCATCCATATCCTTCTACGAGGCCTTTTGGTAGTTTCCCAGACATTTGTGGACTAGGTCTTTCTGTCTCTCAGTGCATTTGCTCCGCGGTTCAGTACAATTACCTCCATCGGCATGCTAATAATCCTATCAAACTATCCATTTTCCCTTTCATCTTTCTTTTATTTTTGGCTATTCAAAGGTGTTGTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

220

Amino Acids

24.75

Weight (kDa)

8.19

Isoelectric Point (pI)

33.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 245
AccII CGCG 1 cut(s) 554
AciI CCGC 4 cut(s) 54, 300, 552, 554
AclWI GGATC 3 cut(s) 46, 64, 272
AcsI RAATTY 1 cut(s) 250
AcuI CTGAAG 1 cut(s) 288
AfaI GTAC 2 cut(s) 170, 563
AgsI TTSAA 2 cut(s) 17, 644
AhdI GACNNNNNGTC 1 cut(s) 241
AjnI CCWGG 1 cut(s) 86
AluBI AGCT 7 cut(s) 13, 99, 108, 145, 308, 322, 403
AluI AGCT 7 cut(s) 13, 99, 108, 145, 308, 322, 403
Alw26I GTCTC 2 cut(s) 290, 538
AlwI GGATC 3 cut(s) 46, 64, 272
AoxI GGCC 2 cut(s) 149, 488
ApeKI GCWGC 1 cut(s) 364
ApoI RAATTY 1 cut(s) 250
AsuNHI GCTAGC 1 cut(s) 376
BanII GRGCYC 2 cut(s) 87, 165
BbvI GCAGC 1 cut(s) 351
BccI CCATC 1 cut(s) 582
BceAI ACGGC 1 cut(s) 365
BciT130I CCWGG 1 cut(s) 88
BclI TGATCA 1 cut(s) 316
BcoDI GTCTC 2 cut(s) 290, 538
BfaI CTAG 3 cut(s) 51, 377, 521
BfmI CTRYAG 1 cut(s) 42
BisI GCNGC 1 cut(s) 365
BlsI GCNGC 1 cut(s) 366
Bme1390I CCNGG 1 cut(s) 88
BmeRI GACNNNNNGTC 1 cut(s) 241
BmiI GGNNCC 1 cut(s) 162
BmrFI CCNGG 1 cut(s) 88
BmtI GCTAGC 1 cut(s) 380
Bpu10I CCTNAGC 1 cut(s) 146
BsaI GGTCTC 1 cut(s) 290
BsaJI CCNNGG 4 cut(s) 86, 227, 286, 552
BsaWI WCCGGW 1 cut(s) 154
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bse1I ACTGG 1 cut(s) 192
BseBI CCWGG 1 cut(s) 88
BseDI CCNNGG 4 cut(s) 86, 227, 286, 552
BseGI GGATG 1 cut(s) 469
BseMII CTCAG 1 cut(s) 551
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 173
BseXI GCAGC 1 cut(s) 351
Bsh1236I CGCG 1 cut(s) 554
BshFI GGCC 2 cut(s) 151, 490
BsiSI CCGG 1 cut(s) 155
BsmAI GTCTC 2 cut(s) 290, 538
BsnI GGCC 2 cut(s) 151, 490
Bso31I GGTCTC 1 cut(s) 290
Bsp1286I GDGCHC 2 cut(s) 87, 165
Bsp143I GATC 4 cut(s) 38, 56, 264, 316
BspACI CCGC 4 cut(s) 54, 300, 552, 554
BspANI GGCC 2 cut(s) 151, 490
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 1 cut(s) 554
BspHI TCATGA 1 cut(s) 76
BspLI GGNNCC 1 cut(s) 162
BspMAI CTGCAG 1 cut(s) 46
BspOI GCTAGC 1 cut(s) 380
BspPI GGATC 3 cut(s) 46, 64, 272
BspTNI GGTCTC 1 cut(s) 290
BsrI ACTGG 1 cut(s) 192
BssECI CCNNGG 4 cut(s) 86, 227, 286, 552
BssMI GATC 4 cut(s) 38, 56, 264, 316
BssT1I CCWWGG 2 cut(s) 227, 286
Bst2UI CCWGG 1 cut(s) 88
Bst4CI ACNGT 1 cut(s) 131
BstC8I GCNNGC 5 cut(s) 46, 224, 369, 378, 582
BstDEI CTNAG 3 cut(s) 104, 146, 537
BstDSI CCRYGG 1 cut(s) 552
BstF5I GGATG 1 cut(s) 469
BstFNI CGCG 1 cut(s) 554
BstKTI GATC 4 cut(s) 41, 59, 267, 319
BstMAI GTCTC 2 cut(s) 290, 538
BstMBI GATC 4 cut(s) 38, 56, 264, 316
BstMWI GCNNNNNNNGC 4 cut(s) 105, 347, 373, 377
BstNI CCWGG 1 cut(s) 88
BstNSI RCATGY 2 cut(s) 65, 584
BstSCI CCNGG 1 cut(s) 86
BstSFI CTRYAG 1 cut(s) 42
BstUI CGCG 1 cut(s) 554
BstV1I GCAGC 1 cut(s) 351
BstX2I RGATCY 2 cut(s) 38, 264
BstYI RGATCY 2 cut(s) 38, 264
BsuRI GGCC 2 cut(s) 151, 490
BtgI CCRYGG 1 cut(s) 552
BtgZI GCGATG 1 cut(s) 262
BtsCI GGATG 1 cut(s) 469
BtsIMutI CAGTG 1 cut(s) 545
Cac8I GCNNGC 5 cut(s) 46, 224, 369, 378, 582
CciI TCATGA 1 cut(s) 76
Cfr42I CCGCGG 1 cut(s) 555
Csp6I GTAC 2 cut(s) 169, 562
CviAII CATG 4 cut(s) 62, 77, 338, 581
CviQI GTAC 2 cut(s) 169, 562
DdeI CTNAG 3 cut(s) 104, 146, 537
DpnI GATC 4 cut(s) 40, 58, 266, 318
DpnII GATC 4 cut(s) 38, 56, 264, 316
DraI TTTAAA 1 cut(s) 333
DriI GACNNNNNGTC 1 cut(s) 241
Eam1105I GACNNNNNGTC 1 cut(s) 241
Eco130I CCWWGG 2 cut(s) 227, 286
Eco147I AGGCCT 1 cut(s) 490
Eco24I GRGCYC 2 cut(s) 87, 165
Eco31I GGTCTC 1 cut(s) 290
Eco57I CTGAAG 1 cut(s) 288
EcoRII CCWGG 1 cut(s) 86
EcoT14I CCWWGG 2 cut(s) 227, 286
EcoT38I GRGCYC 2 cut(s) 87, 165
ErhI CCWWGG 2 cut(s) 227, 286
FaeI CATG 4 cut(s) 65, 80, 341, 584
FalI AAGNNNNNCTT 2 cut(s) 251, 283
FatI CATG 4 cut(s) 61, 76, 337, 580
FbaI TGATCA 1 cut(s) 316
FblI GTMKAC 1 cut(s) 245
Fnu4HI GCNGC 1 cut(s) 365
FokI GGATG 1 cut(s) 456
FriOI GRGCYC 2 cut(s) 87, 165
Fsp4HI GCNGC 1 cut(s) 365
FspBI CTAG 3 cut(s) 51, 377, 521
GluI GCNGC 1 cut(s) 365
HaeIII GGCC 2 cut(s) 151, 490
HapII CCGG 1 cut(s) 155
Hin1II CATG 4 cut(s) 65, 80, 341, 584
HinfI GANTC 1 cut(s) 406
HpaII CCGG 1 cut(s) 155
Hpy166II GTNNAC 2 cut(s) 246, 518
Hpy188I TCNGA 1 cut(s) 256
Hpy188III TCNNGA 1 cut(s) 77
Hpy8I GTNNAC 2 cut(s) 246, 518
HpyAV CCTTC 1 cut(s) 489
HpyCH4III ACNGT 1 cut(s) 131
HpyCH4V TGCA 5 cut(s) 44, 199, 350, 393, 543
HpyF10VI GCNNNNNNNGC 4 cut(s) 105, 347, 373, 377
HpyF3I CTNAG 3 cut(s) 104, 146, 537
Hsp92II CATG 4 cut(s) 65, 80, 341, 584
Ksp22I TGATCA 1 cut(s) 316
KspI CCGCGG 1 cut(s) 555
Kzo9I GATC 4 cut(s) 38, 56, 264, 316
LmnI GCTCC 3 cut(s) 160, 400, 554
LpnPI CCDG 9 cut(s) 30, 73, 100, 109, 168, 205, 218, 353, 519
Lsp1109I GCAGC 1 cut(s) 351
MaeI CTAG 3 cut(s) 51, 377, 521
MalI GATC 4 cut(s) 40, 58, 266, 318
MboI GATC 4 cut(s) 38, 56, 264, 316
MboII GAAGA 1 cut(s) 265
MflI RGATCY 2 cut(s) 38, 264
MhlI GDGCHC 2 cut(s) 87, 165
MluCI AATT 3 cut(s) 115, 250, 565
MnlI CCTC 5 cut(s) 151, 337, 415, 480, 581
MseI TTAA 2 cut(s) 332, 437
MslI CAYNNNNRTG 2 cut(s) 359, 579
MspA1I CMGCKG 2 cut(s) 308, 554
MspI CCGG 1 cut(s) 155
MspR9I CCNGG 1 cut(s) 88
MvaI CCWGG 1 cut(s) 88
MvnI CGCG 1 cut(s) 554
MwoI GCNNNNNNNGC 4 cut(s) 105, 347, 373, 377
NdeII GATC 4 cut(s) 38, 56, 264, 316
NheI GCTAGC 1 cut(s) 376
NlaIII CATG 4 cut(s) 65, 80, 341, 584
NlaIV GGNNCC 1 cut(s) 162
NspI RCATGY 2 cut(s) 65, 584
PaeI GCATGC 1 cut(s) 584
PagI TCATGA 1 cut(s) 76
PceI AGGCCT 1 cut(s) 490
PfeI GAWTC 1 cut(s) 406
PflFI GACNNNGTC 1 cut(s) 522
PkrI GCNGC 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 86
PspGI CCWGG 1 cut(s) 86
PspN4I GGNNCC 1 cut(s) 162
PstI CTGCAG 1 cut(s) 46
PsuI RGATCY 2 cut(s) 38, 264
PsyI GACNNNGTC 1 cut(s) 522
PvuII CAGCTG 1 cut(s) 308
RsaI GTAC 2 cut(s) 170, 563
RsaNI GTAC 2 cut(s) 169, 562
RseI CAYNNNNRTG 2 cut(s) 359, 579
SacII CCGCGG 1 cut(s) 555
SaqAI TTAA 2 cut(s) 332, 437
SatI GCNGC 1 cut(s) 365
Sau3AI GATC 4 cut(s) 38, 56, 264, 316
ScrFI CCNGG 1 cut(s) 88
SduI GDGCHC 2 cut(s) 87, 165
SfcI CTRYAG 1 cut(s) 42
Sfr303I CCGCGG 1 cut(s) 555
SgrBI CCGCGG 1 cut(s) 555
SmiMI CAYNNNNRTG 2 cut(s) 359, 579
SphI GCATGC 1 cut(s) 584
Sse9I AATT 3 cut(s) 115, 250, 565
SseBI AGGCCT 1 cut(s) 490
SsiI CCGC 4 cut(s) 54, 300, 552, 554
SspMI CTAG 3 cut(s) 51, 377, 521
StuI AGGCCT 1 cut(s) 490
StyD4I CCNGG 1 cut(s) 86
StyI CCWWGG 2 cut(s) 227, 286
TaaI ACNGT 1 cut(s) 131
TaqI TCGA 1 cut(s) 210
TasI AATT 3 cut(s) 115, 250, 565
TatI WGTACW 2 cut(s) 168, 561
TfiI GAWTC 1 cut(s) 406
Tru1I TTAA 2 cut(s) 332, 437
Tru9I TTAA 2 cut(s) 332, 437
TscAI CASTG 1 cut(s) 545
TseI GCWGC 1 cut(s) 364
TspDTI ATGAA 3 cut(s) 65, 265, 607
TspRI CASTG 1 cut(s) 545
Tth111I GACNNNGTC 1 cut(s) 522
XapI RAATTY 1 cut(s) 250
XceI RCATGY 2 cut(s) 65, 584
XmiI GTMKAC 1 cut(s) 245
XspI CTAG 3 cut(s) 51, 377, 521
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.