Rmu_sc0001663.1_g000017

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001663.1
OG0000508
Physical Location & Seq
Forward (+)
90163 .. 91438
1276 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001663.1_g000017.1.cds

Sequence Viewer

Length: 759 bp
atggagagccaagaagcttgtgtgcttgagatccgaccttctttttcttcaaagagggctttggagaattggaggaagctatctgctggcctcaagaagcccaacaagcgacagtttctcttcaaccctgcggctcgcgccgtttggattaaggagcaggaggaacacattcagaccgtattgcaatttgtgaatgctcgccaggatcggttgtctttgcttggcagtagttccggtgacgagtcaaggcacacaacaaaggctacttcaacagtatgggtcattttactcaccaacctcggcctagagacattatcggccgtctttgatcaaattgcctctccaagcaagcctgactatgcactattcggaatggtgttggcgattttagctgtgctaatttgcatcttggagctcattcacaagggcaaaaaggaaggagttgagctgaaaagatggggaaagttgtgctggttttattatccacctcctaatgaatcactttttggtacattcccagatatttgtggattagtcattgccatctcacagtatatttgctccactgttcagtatgtttatctgtgcaggcatgctaataaccccatcaaactatccgttttgccggccatctttcttatttgtttggctggttcaaaactaattaggaacagaagctacaccacaaatgagacctttaaacatactactcatcatattacaaagattaatttttgtagattttggcattcagtttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

252

Amino Acids

28.66

Weight (kDa)

9.35

Isoelectric Point (pI)

47.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 138
AciI CCGC 1 cut(s) 131
AclWI GGATC 2 cut(s) 25, 213
AcoI YGGCCR 2 cut(s) 318, 627
AfaI GTAC 1 cut(s) 511
AgsI TTSAA 4 cut(s) 51, 124, 270, 657
AjnI CCWGG 1 cut(s) 201
AjuI GAANNNNNNNTTGG 2 cut(s) 489, 521
AluBI AGCT 6 cut(s) 17, 79, 392, 415, 448, 678
AluI AGCT 6 cut(s) 17, 79, 392, 415, 448, 678
Alw21I GWGCWC 1 cut(s) 417
Alw26I GTCTC 2 cut(s) 302, 686
AlwI GGATC 2 cut(s) 25, 213
AoxI GGCC 4 cut(s) 88, 301, 318, 627
AseI ATTAAT 1 cut(s) 729
Asp700I GAANNNNTTC 1 cut(s) 168
AspLEI GCGC 1 cut(s) 140
AsuHPI GGTGA 2 cut(s) 248, 283
BanII GRGCYC 1 cut(s) 417
Bbv12I GWGCWC 1 cut(s) 417
BccI CCATC 4 cut(s) 450, 551, 614, 638
BceAI ACGGC 2 cut(s) 125, 305
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 328
BcoDI GTCTC 2 cut(s) 302, 686
BfaI CTAG 1 cut(s) 305
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 203
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 47, 77
BsaI GGTCTC 1 cut(s) 686
BsaJI CCNNGG 1 cut(s) 298
BsaWI WCCGGW 1 cut(s) 233
Bse118I RCCGGY 1 cut(s) 625
Bse3DI GCAATG 1 cut(s) 537
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 298
BseMI GCAATG 1 cut(s) 537
BseX3I CGGCCG 1 cut(s) 318
BsgI GTGCAG 1 cut(s) 607
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 321
BshFI GGCC 4 cut(s) 90, 303, 320, 629
BsiEI CGRYCG 1 cut(s) 321
BsiHKAI GWGCWC 1 cut(s) 417
BsiSI CCGG 2 cut(s) 234, 626
BsmAI GTCTC 2 cut(s) 302, 686
BsmI GAATGC 2 cut(s) 199, 748
BsnI GGCC 4 cut(s) 90, 303, 320, 629
Bso31I GGTCTC 1 cut(s) 686
Bsp1286I GDGCHC 1 cut(s) 417
Bsp143I GATC 3 cut(s) 30, 205, 328
BspACI CCGC 1 cut(s) 131
BspANI GGCC 4 cut(s) 90, 303, 320, 629
BspFNI CGCG 1 cut(s) 138
BspPI GGATC 2 cut(s) 25, 213
BspTNI GGTCTC 1 cut(s) 686
BsrDI GCAATG 1 cut(s) 537
BsrFI RCCGGY 1 cut(s) 625
BssAI RCCGGY 1 cut(s) 625
BssECI CCNNGG 1 cut(s) 298
BssMI GATC 3 cut(s) 30, 205, 328
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 5 cut(s) 114, 178, 274, 552, 568
Bst6I CTCTTC 1 cut(s) 125
BstC8I GCNNGC 7 cut(s) 88, 136, 199, 350, 590, 594, 627
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 3 cut(s) 33, 208, 331
BstMAI GTCTC 2 cut(s) 302, 686
BstMBI GATC 3 cut(s) 30, 205, 328
BstMCI CGRYCG 1 cut(s) 321
BstMWI GCNNNNNNNGC 3 cut(s) 106, 137, 389
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 596
BstSCI CCNGG 1 cut(s) 201
BstUI CGCG 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BstZI CGGCCG 1 cut(s) 318
BsuRI GGCC 4 cut(s) 90, 303, 320, 629
BtsIMutI CAGTG 1 cut(s) 564
Cac8I GCNNGC 7 cut(s) 88, 136, 199, 350, 590, 594, 627
CfoI GCGC 1 cut(s) 140
Cfr10I RCCGGY 1 cut(s) 625
Csp6I GTAC 1 cut(s) 510
CviAII CATG 1 cut(s) 593
CviQI GTAC 1 cut(s) 510
DpnI GATC 3 cut(s) 32, 207, 330
DpnII GATC 3 cut(s) 30, 205, 328
DraI TTTAAA 1 cut(s) 700
EaeI YGGCCR 2 cut(s) 318, 627
EagI CGGCCG 1 cut(s) 318
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 415
EclXI CGGCCG 1 cut(s) 318
Eco24I GRGCYC 1 cut(s) 417
Eco31I GGTCTC 1 cut(s) 686
Eco52I CGGCCG 1 cut(s) 318
Eco53kI GAGCTC 1 cut(s) 415
EcoICRI GAGCTC 1 cut(s) 415
EcoRII CCWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 417
FaeI CATG 1 cut(s) 596
FaiI YATR 7 cut(s) 277, 360, 555, 576, 594, 705, 717
FatI CATG 1 cut(s) 592
FbaI TGATCA 1 cut(s) 328
Fnu4HI GCNGC 1 cut(s) 132
FriOI GRGCYC 1 cut(s) 417
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 305
GlaI GCGC 1 cut(s) 139
GluI GCNGC 1 cut(s) 132
HaeIII GGCC 4 cut(s) 90, 303, 320, 629
HapII CCGG 2 cut(s) 234, 626
HhaI GCGC 1 cut(s) 140
Hin1II CATG 1 cut(s) 596
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 2 cut(s) 242, 497
HpaII CCGG 2 cut(s) 234, 626
HphI GGTGA 2 cut(s) 248, 283
Hpy188I TCNGA 3 cut(s) 35, 174, 371
Hpy188III TCNNGA 1 cut(s) 94
HpyAV CCTTC 2 cut(s) 48, 431
HpyCH4III ACNGT 5 cut(s) 114, 178, 274, 552, 568
HpyCH4V TGCA 4 cut(s) 184, 362, 405, 588
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 137, 389
Hsp92II CATG 1 cut(s) 596
HspAI GCGC 1 cut(s) 138
KroI GCCGGC 1 cut(s) 625
KroNI GCCGGC 1 cut(s) 627
Ksp22I TGATCA 1 cut(s) 328
Kzo9I GATC 3 cut(s) 30, 205, 328
LmnI GCTCC 3 cut(s) 154, 412, 566
LweI GCATC 1 cut(s) 414
MaeI CTAG 1 cut(s) 305
MaeIII GTNAC 1 cut(s) 236
MalI GATC 3 cut(s) 32, 207, 330
MboI GATC 3 cut(s) 30, 205, 328
MboII GAAGA 2 cut(s) 39, 112
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 417
MluCI AATT 6 cut(s) 67, 185, 333, 399, 663, 730
MlyI GAGTC 1 cut(s) 251
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 7 cut(s) 48, 66, 101, 154, 308, 349, 498
MroNI GCCGGC 1 cut(s) 625
MroXI GAANNNNTTC 1 cut(s) 168
MseI TTAA 3 cut(s) 150, 699, 729
MspI CCGG 2 cut(s) 234, 626
MspR9I CCNGG 1 cut(s) 203
Mva1269I GAATGC 2 cut(s) 199, 748
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 3 cut(s) 106, 137, 389
NaeI GCCGGC 1 cut(s) 627
NdeII GATC 3 cut(s) 30, 205, 328
NgoMIV GCCGGC 1 cut(s) 625
NlaIII CATG 1 cut(s) 596
NmeAIII GCCGAG 1 cut(s) 279
NmuCI GTSAC 1 cut(s) 236
NspI RCATGY 1 cut(s) 596
PaeI GCATGC 1 cut(s) 596
PctI GAATGC 2 cut(s) 199, 748
PdiI GCCGGC 1 cut(s) 627
PdmI GAANNNNTTC 1 cut(s) 168
PfeI GAWTC 1 cut(s) 497
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
PshBI ATTAAT 1 cut(s) 729
Psp124BI GAGCTC 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PsrI GAACNNNNNNTAC 2 cut(s) 662, 694
PsuI RGATCY 1 cut(s) 30
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
SacI GAGCTC 1 cut(s) 417
SaqAI TTAA 3 cut(s) 150, 699, 729
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 3 cut(s) 30, 205, 328
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 1 cut(s) 417
SfaNI GCATC 1 cut(s) 414
SmlI CTYRAG 2 cut(s) 26, 92
SmoI CTYRAG 2 cut(s) 26, 92
SphI GCATGC 1 cut(s) 596
Sse9I AATT 6 cut(s) 67, 185, 333, 399, 663, 730
SsiI CCGC 1 cut(s) 131
SspMI CTAG 1 cut(s) 305
SstI GAGCTC 1 cut(s) 417
StyD4I CCNGG 1 cut(s) 201
TaaI ACNGT 5 cut(s) 114, 178, 274, 552, 568
TasI AATT 6 cut(s) 67, 185, 333, 399, 663, 730
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 497
Tru1I TTAA 3 cut(s) 150, 699, 729
Tru9I TTAA 3 cut(s) 150, 699, 729
TscAI CASTG 1 cut(s) 571
TseFI GTSAC 1 cut(s) 236
Tsp45I GTSAC 1 cut(s) 236
TspDTI ATGAA 1 cut(s) 510
TspGWI ACGGA 1 cut(s) 607
TspRI CASTG 1 cut(s) 571
VspI ATTAAT 1 cut(s) 729
XceI RCATGY 1 cut(s) 596
XmnI GAANNNNTTC 1 cut(s) 168
XspI CTAG 1 cut(s) 305
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.