Rroxscaffold_7G00192430

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000508
Physical Location & Seq
Forward (+)
33614457 .. 33617904
3448 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00192430.1

Sequence Viewer

Length: 858 bp
ATGGTGTTGAAGAAGCGAAGAGCATCTATTCCATTACGGATATCTATTCCTTTTACTTCTCGGGCGTGTGATGTCTTCCTAAGTTGCGAGTATGAAGACACCTTCTCCTCCCACTTGTACCACAAATTGCAGTCTCAAGGAATTAGTACTTTCCAGGATGGCCAACAATTGCAAAGAAACACAACTCCTCTTGAGCTCTTTGGTGCGATCGAAGAATCGAAATTTGCCATTGTTGTTCTCTCGCAAAACTATGCTTCTTCGCCCCAGCGCTTGAATGAACTTTCAAAAATTCTAGAGTGCATGAAAGACAGAAACAGGATTCTGCCGGTATTTCGTGATGTGAATCTGTTCCATGTCCAAAAACAAAAAGGGGCTTTTGAGAAAGCATTTGAAAAGCATGGAGAAAGGTTTCAGGATGACTTGGAAAAGGTTCGAGCCTGGAGAGATGCTTTAACCCAAGTGTGCAATTTTGCTGGATGGACTACCAATGACAGGCCTTTAGCTACAAAAGATAGACCAACTGATGATCTGGGCATTCATTATTTCCTTTTCTTGATGGTCGCATTCACTAGCATTAGTGTTAATGAGGACAACCATGACCATAACCATCCAATAAACATTACAGATGTGACGGCTACTTCAGTAGAGTGGAAGTTTTTGATTACTAACCTTGGCCTAGAGATTTTGTCAGCTGCTTTTGATCAGGCTTCCTCACCAAGTAAGCCACACTATGCACTATTTGAGGGACTTTTGAGAAAGCATTTGATCGACAAGCCTGAAGAAAGGTTTCGGGATGACTTGGAAAAGGTTCAAGCCTGGAGAGATACTTTAATCAATGTGTGCAATTTTGCTGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

285

Amino Acids

32.85

Weight (kDa)

6.37

Isoelectric Point (pI)

48.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 25 - 163 7.6e-31 TIR domain
TIR_2 PF13676 25 - 114 1.9e-11 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 160
AcsI RAATTY 2 cut(s) 221, 288
AcuI CTGAAG 2 cut(s) 624, 798
AfaI GTAC 2 cut(s) 119, 148
AfeI AGCGCT 1 cut(s) 269
AfiI CCNNNNNNNGG 1 cut(s) 492
AgsI TTSAA 5 cut(s) 10, 274, 285, 392, 812
AjnI CCWGG 3 cut(s) 153, 437, 815
AluBI AGCT 3 cut(s) 196, 503, 692
AluI AGCT 3 cut(s) 196, 503, 692
Alw21I GWGCWC 1 cut(s) 198
Alw26I GTCTC 1 cut(s) 138
Ama87I CYCGRG 1 cut(s) 60
Aor51HI AGCGCT 1 cut(s) 269
AoxI GGCC 3 cut(s) 160, 494, 673
ApeKI GCWGC 1 cut(s) 692
ApoI RAATTY 2 cut(s) 221, 288
Asp700I GAANNNNTTC 5 cut(s) 347, 408, 429, 786, 807
AspLEI GCGC 1 cut(s) 270
AsuHPI GGTGA 1 cut(s) 705
AvaI CYCGRG 1 cut(s) 60
BalI TGGCCA 1 cut(s) 162
BanII GRGCYC 1 cut(s) 198
BbsI GAAGAC 2 cut(s) 67, 102
Bbv12I GWGCWC 1 cut(s) 198
BbvI GCAGC 1 cut(s) 679
BccI CCATC 4 cut(s) 152, 471, 550, 615
BceAI ACGGC 1 cut(s) 648
BciT130I CCWGG 3 cut(s) 155, 439, 817
BclI TGATCA 1 cut(s) 700
BcoDI GTCTC 1 cut(s) 138
BfaI CTAG 3 cut(s) 293, 570, 677
BfoI RGCGCY 1 cut(s) 271
BisI GCNGC 1 cut(s) 693
BlsI GCNGC 1 cut(s) 694
BmcAI AGTACT 1 cut(s) 148
Bme1390I CCNGG 3 cut(s) 155, 439, 817
BmeT110I CYCGRG 1 cut(s) 60
BmrFI CCNGG 3 cut(s) 155, 439, 817
BmsI GCATC 2 cut(s) 32, 436
BpiI GAAGAC 2 cut(s) 67, 102
BpmI CTGGAG 2 cut(s) 460, 838
BpuEI CTTGAG 2 cut(s) 120, 212
BsaBI GATNNNNATC 1 cut(s) 342
BsaJI CCNNGG 1 cut(s) 670
BsaXI ACNNNNNCTCC 2 cut(s) 89, 119
Bsc4I CCNNNNNNNGG 1 cut(s) 492
Bse118I RCCGGY 1 cut(s) 325
Bse8I GATNNNNATC 1 cut(s) 342
BseBI CCWGG 3 cut(s) 155, 439, 817
BseDI CCNNGG 1 cut(s) 670
BseGI GGATG 5 cut(s) 163, 421, 482, 607, 799
BseJI GATNNNNATC 1 cut(s) 342
BseLI CCNNNNNNNGG 1 cut(s) 492
BseRI GAGGAG 2 cut(s) 97, 177
BseXI GCAGC 1 cut(s) 679
BseYI CCCAGC 1 cut(s) 264
Bsh1285I CGRYCG 1 cut(s) 210
BshFI GGCC 3 cut(s) 162, 496, 675
BsiEI CGRYCG 1 cut(s) 210
BsiHKAI GWGCWC 1 cut(s) 198
BsiHKCI CYCGRG 1 cut(s) 60
BsiSI CCGG 1 cut(s) 326
BslFI GGGAC 1 cut(s) 759
BslI CCNNNNNNNGG 1 cut(s) 492
BsmAI GTCTC 1 cut(s) 138
BsmFI GGGAC 1 cut(s) 759
BsmI GAATGC 2 cut(s) 534, 563
BsnI GGCC 3 cut(s) 162, 496, 675
BsoBI CYCGRG 1 cut(s) 60
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 4 cut(s) 207, 526, 700, 765
BspANI GGCC 3 cut(s) 162, 496, 675
BspQI GCTCTTC 1 cut(s) 13
BsrFI RCCGGY 1 cut(s) 325
BssAI RCCGGY 1 cut(s) 325
BssECI CCNNGG 1 cut(s) 670
BssMI GATC 4 cut(s) 207, 526, 700, 765
BssT1I CCWWGG 1 cut(s) 670
Bst2UI CCWGG 3 cut(s) 155, 439, 817
Bst6I CTCTTC 1 cut(s) 13
BstDEI CTNAG 1 cut(s) 80
BstF5I GGATG 5 cut(s) 163, 421, 482, 607, 799
BstH2I RGCGCY 1 cut(s) 271
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 4 cut(s) 210, 529, 703, 768
BstMAI GTCTC 1 cut(s) 138
BstMBI GATC 4 cut(s) 207, 526, 700, 765
BstMCI CGRYCG 1 cut(s) 210
BstNI CCWGG 3 cut(s) 155, 439, 817
BstSCI CCNGG 3 cut(s) 153, 437, 815
BstV1I GCAGC 1 cut(s) 679
BstV2I GAAGAC 2 cut(s) 67, 102
BsuRI GGCC 3 cut(s) 162, 496, 675
BtsCI GGATG 5 cut(s) 163, 421, 482, 607, 799
CfoI GCGC 1 cut(s) 270
Cfr10I RCCGGY 1 cut(s) 325
Csp6I GTAC 2 cut(s) 118, 147
CviAII CATG 4 cut(s) 301, 353, 398, 596
CviQI GTAC 2 cut(s) 118, 147
DdeI CTNAG 1 cut(s) 80
DpnI GATC 4 cut(s) 209, 528, 702, 767
DpnII GATC 4 cut(s) 207, 526, 700, 765
EaeI YGGCCR 1 cut(s) 160
Eam1104I CTCTTC 1 cut(s) 13
EarI CTCTTC 1 cut(s) 13
Ecl136II GAGCTC 1 cut(s) 196
Eco130I CCWWGG 1 cut(s) 670
Eco147I AGGCCT 1 cut(s) 496
Eco24I GRGCYC 1 cut(s) 198
Eco32I GATATC 1 cut(s) 42
Eco47III AGCGCT 1 cut(s) 269
Eco53kI GAGCTC 1 cut(s) 196
Eco57I CTGAAG 2 cut(s) 624, 798
Eco88I CYCGRG 1 cut(s) 60
EcoICRI GAGCTC 1 cut(s) 196
EcoRII CCWGG 3 cut(s) 153, 437, 815
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 1 cut(s) 670
EcoT38I GRGCYC 1 cut(s) 198
ErhI CCWWGG 1 cut(s) 670
FaeI CATG 4 cut(s) 304, 356, 401, 599
FaiI YATR 8 cut(s) 93, 252, 302, 354, 399, 597, 603, 732
FaqI GGGAC 1 cut(s) 759
FatI CATG 4 cut(s) 300, 352, 397, 595
FbaI TGATCA 1 cut(s) 700
Fnu4HI GCNGC 1 cut(s) 693
FokI GGATG 5 cut(s) 170, 428, 489, 594, 806
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 1 cut(s) 693
FspBI CTAG 3 cut(s) 293, 570, 677
GlaI GCGC 1 cut(s) 269
GluI GCNGC 1 cut(s) 693
GsaI CCCAGC 1 cut(s) 268
GsuI CTGGAG 2 cut(s) 460, 838
HaeII RGCGCY 1 cut(s) 271
HaeIII GGCC 3 cut(s) 162, 496, 675
HapII CCGG 1 cut(s) 326
HhaI GCGC 1 cut(s) 270
Hin1II CATG 4 cut(s) 304, 356, 401, 599
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HinfI GANTC 3 cut(s) 215, 319, 343
HpaII CCGG 1 cut(s) 326
HphI GGTGA 1 cut(s) 705
Hpy188III TCNNGA 6 cut(s) 191, 293, 335, 413, 553, 791
HpyAV CCTTC 1 cut(s) 112
HpyCH4V TGCA 6 cut(s) 130, 172, 300, 465, 734, 843
HpyF3I CTNAG 1 cut(s) 80
Hsp92II CATG 4 cut(s) 304, 356, 401, 599
HspAI GCGC 1 cut(s) 268
Ksp22I TGATCA 1 cut(s) 700
Kzo9I GATC 4 cut(s) 207, 526, 700, 765
LguI GCTCTTC 1 cut(s) 13
Lsp1109I GCAGC 1 cut(s) 679
LweI GCATC 2 cut(s) 32, 436
MaeI CTAG 3 cut(s) 293, 570, 677
MaeIII GTNAC 1 cut(s) 628
MalI GATC 4 cut(s) 209, 528, 702, 767
MboI GATC 4 cut(s) 207, 526, 700, 765
MboII GAAGA 7 cut(s) 22, 30, 67, 107, 224, 249, 791
MfeI CAATTG 1 cut(s) 167
MhlI GDGCHC 1 cut(s) 198
MlsI TGGCCA 1 cut(s) 162
MluCI AATT 7 cut(s) 125, 141, 167, 221, 288, 466, 844
MluNI TGGCCA 1 cut(s) 162
MnlI CCTC 5 cut(s) 118, 198, 580, 721, 736
Mox20I TGGCCA 1 cut(s) 162
MroXI GAANNNNTTC 5 cut(s) 347, 408, 429, 786, 807
MscI TGGCCA 1 cut(s) 162
MseI TTAA 3 cut(s) 452, 582, 830
Msp20I TGGCCA 1 cut(s) 162
MspA1I CMGCKG 1 cut(s) 692
MspI CCGG 1 cut(s) 326
MspR9I CCNGG 3 cut(s) 155, 439, 817
MunI CAATTG 1 cut(s) 167
Mva1269I GAATGC 2 cut(s) 534, 563
MvaI CCWGG 3 cut(s) 155, 439, 817
NdeII GATC 4 cut(s) 207, 526, 700, 765
NlaIII CATG 4 cut(s) 304, 356, 401, 599
NmuCI GTSAC 1 cut(s) 628
PceI AGGCCT 1 cut(s) 496
PciSI GCTCTTC 1 cut(s) 13
PctI GAATGC 2 cut(s) 534, 563
PdmI GAANNNNTTC 5 cut(s) 347, 408, 429, 786, 807
PfeI GAWTC 3 cut(s) 215, 319, 343
PfoI TCCNGGA 1 cut(s) 153
PkrI GCNGC 1 cut(s) 694
Ple19I CGATCG 1 cut(s) 210
Psp124BI GAGCTC 1 cut(s) 198
Psp6I CCWGG 3 cut(s) 153, 437, 815
PspFI CCCAGC 1 cut(s) 264
PspGI CCWGG 3 cut(s) 153, 437, 815
PvuI CGATCG 1 cut(s) 210
PvuII CAGCTG 1 cut(s) 692
RsaI GTAC 2 cut(s) 119, 148
RsaNI GTAC 2 cut(s) 118, 147
SacI GAGCTC 1 cut(s) 198
SapI GCTCTTC 1 cut(s) 13
SaqAI TTAA 3 cut(s) 452, 582, 830
SatI GCNGC 1 cut(s) 693
Sau3AI GATC 4 cut(s) 207, 526, 700, 765
ScaI AGTACT 1 cut(s) 148
ScrFI CCNGG 3 cut(s) 155, 439, 817
SduI GDGCHC 1 cut(s) 198
SetI ASST 9 cut(s) 104, 198, 410, 432, 505, 672, 694, 788, 810
SfaNI GCATC 2 cut(s) 32, 436
SmlI CTYRAG 2 cut(s) 135, 191
SmoI CTYRAG 2 cut(s) 135, 191
Sse9I AATT 7 cut(s) 125, 141, 167, 221, 288, 466, 844
SseBI AGGCCT 1 cut(s) 496
SspMI CTAG 3 cut(s) 293, 570, 677
SstI GAGCTC 1 cut(s) 198
StuI AGGCCT 1 cut(s) 496
StyD4I CCNGG 3 cut(s) 153, 437, 815
StyI CCWWGG 1 cut(s) 670
TaqI TCGA 4 cut(s) 210, 218, 433, 768
TasI AATT 7 cut(s) 125, 141, 167, 221, 288, 466, 844
TatI WGTACW 1 cut(s) 146
TfiI GAWTC 3 cut(s) 215, 319, 343
Tru1I TTAA 3 cut(s) 452, 582, 830
Tru9I TTAA 3 cut(s) 452, 582, 830
TseFI GTSAC 1 cut(s) 628
TseI GCWGC 1 cut(s) 692
Tsp45I GTSAC 1 cut(s) 628
TspDTI ATGAA 4 cut(s) 108, 291, 317, 527
TspGWI ACGGA 1 cut(s) 52
XapI RAATTY 2 cut(s) 221, 288
XbaI TCTAGA 1 cut(s) 292
XmnI GAANNNNTTC 5 cut(s) 347, 408, 429, 786, 807
XspI CTAG 3 cut(s) 293, 570, 677
ZrmI AGTACT 1 cut(s) 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.