RH6DG081500

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: Sequence Only
Biological Identity
rosa_samantha
Unknown
OG0000508
Physical Location & Seq
Reverse (-)
0 .. 0
1 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG081500.1

Sequence Viewer

Length: 774 bp
ATGGAGAGCCAAGAAGCTTGTGTGATTGAGATCCGACCTTCTTTTTCTTCAAAGAGGGCTTTGGAGAATTGGAGGAAGCTATCTGCTGGCCTCAAGAAGCCCAACAAGCGACAGTTTCTCTTCAACCCTGCGGCTCGCGCCCTTTGGATCAAGGAGCAGGAGGAACACATTCAGACCGTATTGCAATTTGTGAATGCTCGCCAGGATCGGTTGTCTAACAGCAGGTTGTCTTTGCTTGGCAGTAGTTCCGGTGACGAGTCAAGGCACACAACAAAGGCTACTTCAACAGTATGGGTCTTTTTACTCACCAACCTCGGCCTAGAGACATTATCGGCCGTCTTTGATCAAATTGCCTCTCCAAGCAAGCCTGACTATGCACTATTCGGAATGGTGTTGGCGATTTTAGCTGTGCTAATTTGCATCTTGGAGCTCATTCACAAGGGCAAAAAGGAAGGAGTTGAGCTGAAAAGATGGGGAAAGTTGTGCTGGTTTTATTATCCACCTCCTAATGAATCACTTTTTGGTACTTTCCCAGATATTTGTGGATTAGTCATTGCCATCTCACAGTATATTTGCTCCACTGTTCAGTATGTTTATCTGTGCAGGCATGCTAATAACCCCATCAAACTATCCGTTTTGCCGGCCATCTTTCTTATTTGTTTGGCTGGTTCAAAACTAATTAGGAACAGAAGCTACACCACAAATGAGACCTTTAAACATACTACTCATCATATAACGAAGATTAATTTTTGTAGATTTTGGCATTCAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

257

Amino Acids

29.26

Weight (kDa)

9.43

Isoelectric Point (pI)

49.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 213
AccII CGCG 1 cut(s) 138
AciI CCGC 1 cut(s) 131
AclWI GGATC 3 cut(s) 25, 155, 213
AcoI YGGCCR 2 cut(s) 333, 642
AfaI GTAC 1 cut(s) 526
AgsI TTSAA 4 cut(s) 51, 124, 285, 672
AjnI CCWGG 1 cut(s) 201
AjuI GAANNNNNNNTTGG 2 cut(s) 504, 536
AluBI AGCT 6 cut(s) 17, 79, 407, 430, 463, 693
AluI AGCT 6 cut(s) 17, 79, 407, 430, 463, 693
Alw21I GWGCWC 1 cut(s) 432
Alw26I GTCTC 2 cut(s) 317, 701
AlwI GGATC 3 cut(s) 25, 155, 213
AoxI GGCC 4 cut(s) 88, 316, 333, 642
AseI ATTAAT 1 cut(s) 744
Asp700I GAANNNNTTC 1 cut(s) 168
AspLEI GCGC 1 cut(s) 140
AsuHPI GGTGA 2 cut(s) 263, 298
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 432
BccI CCATC 4 cut(s) 465, 566, 629, 653
BceAI ACGGC 1 cut(s) 320
BciT130I CCWGG 1 cut(s) 203
BclI TGATCA 1 cut(s) 343
BcoDI GTCTC 2 cut(s) 317, 701
BfaI CTAG 1 cut(s) 320
BfuAI ACCTGC 1 cut(s) 213
BisI GCNGC 1 cut(s) 132
BlsI GCNGC 1 cut(s) 133
Bme1390I CCNGG 1 cut(s) 203
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 1 cut(s) 429
BpuEI CTTGAG 1 cut(s) 77
BsaBI GATNNNNATC 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 701
BsaJI CCNNGG 1 cut(s) 313
BsaWI WCCGGW 1 cut(s) 248
Bse118I RCCGGY 1 cut(s) 640
Bse3DI GCAATG 1 cut(s) 552
Bse8I GATNNNNATC 1 cut(s) 29
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 1 cut(s) 313
BseJI GATNNNNATC 1 cut(s) 29
BseMI GCAATG 1 cut(s) 552
BseX3I CGGCCG 1 cut(s) 333
BsgI GTGCAG 1 cut(s) 622
Bsh1236I CGCG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 336
BshFI GGCC 4 cut(s) 90, 318, 335, 644
BsiEI CGRYCG 1 cut(s) 336
BsiHKAI GWGCWC 1 cut(s) 432
BsiSI CCGG 2 cut(s) 249, 641
BsmAI GTCTC 2 cut(s) 317, 701
BsmI GAATGC 2 cut(s) 199, 763
BsnI GGCC 4 cut(s) 90, 318, 335, 644
Bso31I GGTCTC 1 cut(s) 701
Bsp1286I GDGCHC 1 cut(s) 432
Bsp143I GATC 4 cut(s) 30, 147, 205, 343
BspACI CCGC 1 cut(s) 131
BspANI GGCC 4 cut(s) 90, 318, 335, 644
BspFNI CGCG 1 cut(s) 138
BspMI ACCTGC 1 cut(s) 213
BspPI GGATC 3 cut(s) 25, 155, 213
BspTNI GGTCTC 1 cut(s) 701
BsrDI GCAATG 1 cut(s) 552
BsrFI RCCGGY 1 cut(s) 640
BssAI RCCGGY 1 cut(s) 640
BssECI CCNNGG 1 cut(s) 313
BssMI GATC 4 cut(s) 30, 147, 205, 343
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 5 cut(s) 114, 178, 289, 567, 583
Bst6I CTCTTC 1 cut(s) 125
BstC8I GCNNGC 7 cut(s) 88, 136, 199, 365, 605, 609, 642
BstFNI CGCG 1 cut(s) 138
BstHHI GCGC 1 cut(s) 140
BstKTI GATC 4 cut(s) 33, 150, 208, 346
BstMAI GTCTC 2 cut(s) 317, 701
BstMBI GATC 4 cut(s) 30, 147, 205, 343
BstMCI CGRYCG 1 cut(s) 336
BstMWI GCNNNNNNNGC 3 cut(s) 106, 137, 404
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 611
BstSCI CCNGG 1 cut(s) 201
BstUI CGCG 1 cut(s) 138
BstX2I RGATCY 1 cut(s) 30
BstYI RGATCY 1 cut(s) 30
BstZI CGGCCG 1 cut(s) 333
BsuRI GGCC 4 cut(s) 90, 318, 335, 644
BtsIMutI CAGTG 1 cut(s) 579
BveI ACCTGC 1 cut(s) 213
Cac8I GCNNGC 7 cut(s) 88, 136, 199, 365, 605, 609, 642
CfoI GCGC 1 cut(s) 140
Cfr10I RCCGGY 1 cut(s) 640
Csp6I GTAC 1 cut(s) 525
CviAII CATG 1 cut(s) 608
CviQI GTAC 1 cut(s) 525
DpnI GATC 4 cut(s) 32, 149, 207, 345
DpnII GATC 4 cut(s) 30, 147, 205, 343
DraI TTTAAA 1 cut(s) 715
EaeI YGGCCR 2 cut(s) 333, 642
EagI CGGCCG 1 cut(s) 333
Eam1104I CTCTTC 1 cut(s) 125
EarI CTCTTC 1 cut(s) 125
Ecl136II GAGCTC 1 cut(s) 430
EclXI CGGCCG 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 432
Eco31I GGTCTC 1 cut(s) 701
Eco52I CGGCCG 1 cut(s) 333
Eco53kI GAGCTC 1 cut(s) 430
EcoICRI GAGCTC 1 cut(s) 430
EcoRII CCWGG 1 cut(s) 201
EcoT38I GRGCYC 1 cut(s) 432
FaeI CATG 1 cut(s) 611
FaiI YATR 8 cut(s) 292, 375, 570, 591, 609, 720, 732, 734
FatI CATG 1 cut(s) 607
FbaI TGATCA 1 cut(s) 343
Fnu4HI GCNGC 1 cut(s) 132
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 1 cut(s) 132
FspBI CTAG 1 cut(s) 320
GlaI GCGC 1 cut(s) 139
GluI GCNGC 1 cut(s) 132
HaeIII GGCC 4 cut(s) 90, 318, 335, 644
HapII CCGG 2 cut(s) 249, 641
HhaI GCGC 1 cut(s) 140
Hin1II CATG 1 cut(s) 611
Hin6I GCGC 1 cut(s) 138
HinP1I GCGC 1 cut(s) 138
HindIII AAGCTT 1 cut(s) 15
HinfI GANTC 2 cut(s) 257, 512
HpaII CCGG 2 cut(s) 249, 641
HphI GGTGA 2 cut(s) 263, 298
Hpy188I TCNGA 3 cut(s) 35, 174, 386
Hpy188III TCNNGA 1 cut(s) 94
HpyAV CCTTC 2 cut(s) 48, 446
HpyCH4III ACNGT 5 cut(s) 114, 178, 289, 567, 583
HpyCH4V TGCA 4 cut(s) 184, 377, 420, 603
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 137, 404
Hsp92II CATG 1 cut(s) 611
HspAI GCGC 1 cut(s) 138
KroI GCCGGC 1 cut(s) 640
KroNI GCCGGC 1 cut(s) 642
Ksp22I TGATCA 1 cut(s) 343
Kzo9I GATC 4 cut(s) 30, 147, 205, 343
LmnI GCTCC 3 cut(s) 154, 427, 581
LweI GCATC 1 cut(s) 429
MaeI CTAG 1 cut(s) 320
MaeIII GTNAC 1 cut(s) 251
MalI GATC 4 cut(s) 32, 149, 207, 345
MboI GATC 4 cut(s) 30, 147, 205, 343
MboII GAAGA 3 cut(s) 39, 112, 751
MflI RGATCY 1 cut(s) 30
MhlI GDGCHC 1 cut(s) 432
MluCI AATT 6 cut(s) 67, 185, 348, 414, 678, 745
MlyI GAGTC 1 cut(s) 266
MmeI TCCRAC 1 cut(s) 58
MnlI CCTC 7 cut(s) 48, 66, 101, 154, 323, 364, 513
MroNI GCCGGC 1 cut(s) 640
MroXI GAANNNNTTC 1 cut(s) 168
MseI TTAA 2 cut(s) 714, 744
MspI CCGG 2 cut(s) 249, 641
MspR9I CCNGG 1 cut(s) 203
Mva1269I GAATGC 2 cut(s) 199, 763
MvaI CCWGG 1 cut(s) 203
MvnI CGCG 1 cut(s) 138
MwoI GCNNNNNNNGC 3 cut(s) 106, 137, 404
NaeI GCCGGC 1 cut(s) 642
NdeII GATC 4 cut(s) 30, 147, 205, 343
NgoMIV GCCGGC 1 cut(s) 640
NlaIII CATG 1 cut(s) 611
NmeAIII GCCGAG 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 251
NspI RCATGY 1 cut(s) 611
PaeI GCATGC 1 cut(s) 611
PctI GAATGC 2 cut(s) 199, 763
PdiI GCCGGC 1 cut(s) 642
PdmI GAANNNNTTC 1 cut(s) 168
PfeI GAWTC 1 cut(s) 512
PkrI GCNGC 1 cut(s) 133
PleI GAGTC 1 cut(s) 265
PpsI GAGTC 1 cut(s) 265
PshBI ATTAAT 1 cut(s) 744
Psp124BI GAGCTC 1 cut(s) 432
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PsrI GAACNNNNNNTAC 2 cut(s) 677, 709
PsuI RGATCY 1 cut(s) 30
RsaI GTAC 1 cut(s) 526
RsaNI GTAC 1 cut(s) 525
SacI GAGCTC 1 cut(s) 432
SaqAI TTAA 2 cut(s) 714, 744
SatI GCNGC 1 cut(s) 132
Sau3AI GATC 4 cut(s) 30, 147, 205, 343
SchI GAGTC 1 cut(s) 266
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 1 cut(s) 432
SfaNI GCATC 1 cut(s) 429
SmlI CTYRAG 1 cut(s) 92
SmoI CTYRAG 1 cut(s) 92
SphI GCATGC 1 cut(s) 611
Sse9I AATT 6 cut(s) 67, 185, 348, 414, 678, 745
SsiI CCGC 1 cut(s) 131
SspMI CTAG 1 cut(s) 320
SstI GAGCTC 1 cut(s) 432
StyD4I CCNGG 1 cut(s) 201
TaaI ACNGT 5 cut(s) 114, 178, 289, 567, 583
TasI AATT 6 cut(s) 67, 185, 348, 414, 678, 745
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 1 cut(s) 512
Tru1I TTAA 2 cut(s) 714, 744
Tru9I TTAA 2 cut(s) 714, 744
TscAI CASTG 1 cut(s) 586
TseFI GTSAC 1 cut(s) 251
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 1 cut(s) 525
TspGWI ACGGA 1 cut(s) 622
TspRI CASTG 1 cut(s) 586
VspI ATTAAT 1 cut(s) 744
XceI RCATGY 1 cut(s) 611
XmnI GAANNNNTTC 1 cut(s) 168
XspI CTAG 1 cut(s) 320
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.