Prupe.5G012400_v2.0.a1

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000508
Physical Location & Seq
Reverse (-)
1338070 .. 1338581
512 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G012400.1

Sequence Viewer

Length: 453 bp
ATGAGGCCTACTTCAGCATCAGTAGAGTGGATATTTTTAGCCACTTACCTTGTCCTAGAGATCTTGTCAGCTGCTTGTGATCAGGCTTCCTCCCCAAGAAGACCCCACTTTGCACTATTTGGTATGTTGTTGGCTATTGCAGCTGTGTTCATTTCCATCGGGGAGCTAATTTACAGAGGTAAAAGGGAAAGAGTTGTGTTGAGGAGACGGGGAATGCTCTGGTGGTTTTATCATCCACCACCTCCTCGACATACGCCTTTTGGTACTCTTCCTGACATTTATGGACAAGTTGCTGGCATCTCACAGTGCATTTGCTCTATAGTTCAGTATGTTTACTGCCTTCGGCATGCTGATAGTCCTTTCAAAGCATCCCTTTTGCCTGCCATATTTCTTATCTGTTTAGTTGGTTCAAGACTAAGTAATAACCGTATGAACGCCAGCTTTTTAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

151

Amino Acids

16.91

Weight (kDa)

9.72

Isoelectric Point (pI)

52.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfaI GTAC 1 cut(s) 265
AgsI TTSAA 2 cut(s) 364, 411
AluBI AGCT 4 cut(s) 71, 143, 166, 441
AluI AGCT 4 cut(s) 71, 143, 166, 441
Alw26I GTCTC 1 cut(s) 199
AoxI GGCC 1 cut(s) 5
ApeKI GCWGC 2 cut(s) 71, 140
BbsI GAAGAC 1 cut(s) 106
BbvI GCAGC 2 cut(s) 58, 152
BccI CCATC 1 cut(s) 164
BclI TGATCA 1 cut(s) 79
BcoDI GTCTC 1 cut(s) 199
BfaI CTAG 1 cut(s) 56
BfmI CTRYAG 1 cut(s) 318
BglII AGATCT 1 cut(s) 60
BisI GCNGC 2 cut(s) 72, 141
BlsI GCNGC 2 cut(s) 73, 142
BmsI GCATC 3 cut(s) 26, 306, 377
BpiI GAAGAC 1 cut(s) 106
BseGI GGATG 2 cut(s) 232, 368
BseRI GAGGAG 2 cut(s) 217, 234
BseXI GCAGC 2 cut(s) 58, 152
BshFI GGCC 1 cut(s) 7
BsmAI GTCTC 1 cut(s) 199
BsmBI CGTCTC 1 cut(s) 199
BsmI GAATGC 1 cut(s) 219
BsnI GGCC 1 cut(s) 7
Bsp143I GATC 2 cut(s) 60, 79
BspANI GGCC 1 cut(s) 7
BssMI GATC 2 cut(s) 60, 79
Bst4CI ACNGT 2 cut(s) 306, 428
Bst6I CTCTTC 1 cut(s) 273
BstC8I GCNNGC 4 cut(s) 295, 348, 381, 439
BstDEI CTNAG 1 cut(s) 416
BstF5I GGATG 2 cut(s) 232, 368
BstKTI GATC 2 cut(s) 63, 82
BstMAI GTCTC 1 cut(s) 199
BstMBI GATC 2 cut(s) 60, 79
BstMWI GCNNNNNNNGC 1 cut(s) 140
BstNSI RCATGY 1 cut(s) 350
BstSFI CTRYAG 1 cut(s) 318
BstV1I GCAGC 2 cut(s) 58, 152
BstV2I GAAGAC 1 cut(s) 106
BstX2I RGATCY 1 cut(s) 60
BstYI RGATCY 1 cut(s) 60
BsuRI GGCC 1 cut(s) 7
BtsCI GGATG 2 cut(s) 232, 368
BtsIMutI CAGTG 1 cut(s) 311
Cac8I GCNNGC 4 cut(s) 295, 348, 381, 439
Csp6I GTAC 1 cut(s) 264
CspCI CAANNNNNGTGG 2 cut(s) 31, 66
CviAII CATG 1 cut(s) 347
CviJI RGCY 8 cut(s) 7, 41, 71, 86, 134, 143, 166, 441
CviKI_1 RGCY 8 cut(s) 7, 41, 71, 86, 134, 143, 166, 441
CviQI GTAC 1 cut(s) 264
DdeI CTNAG 1 cut(s) 416
DpnI GATC 2 cut(s) 62, 81
DpnII GATC 2 cut(s) 60, 79
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco147I AGGCCT 1 cut(s) 7
Esp3I CGTCTC 1 cut(s) 199
FaeI CATG 1 cut(s) 350
FaiI YATR 8 cut(s) 125, 252, 282, 320, 330, 348, 386, 431
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FatI CATG 1 cut(s) 346
FbaI TGATCA 1 cut(s) 79
Fnu4HI GCNGC 2 cut(s) 72, 141
FokI GGATG 2 cut(s) 219, 355
Fsp4HI GCNGC 2 cut(s) 72, 141
FspBI CTAG 1 cut(s) 56
GluI GCNGC 2 cut(s) 72, 141
HaeIII GGCC 1 cut(s) 7
Hin1II CATG 1 cut(s) 350
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 1 cut(s) 452
Hpy188III TCNNGA 2 cut(s) 272, 411
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 1 cut(s) 350
HpyCH4III ACNGT 2 cut(s) 306, 428
HpyCH4V TGCA 3 cut(s) 113, 140, 309
HpyF10VI GCNNNNNNNGC 1 cut(s) 140
HpyF3I CTNAG 1 cut(s) 416
Hsp92II CATG 1 cut(s) 350
Ksp22I TGATCA 1 cut(s) 79
Kzo9I GATC 2 cut(s) 60, 79
LmnI GCTCC 1 cut(s) 163
LpnPI CCDG 5 cut(s) 68, 205, 279, 285, 393
Lsp1109I GCAGC 2 cut(s) 58, 152
LweI GCATC 3 cut(s) 26, 306, 377
MaeI CTAG 1 cut(s) 56
MalI GATC 2 cut(s) 62, 81
MboI GATC 2 cut(s) 60, 79
MboII GAAGA 2 cut(s) 111, 260
MflI RGATCY 1 cut(s) 60
MluCI AATT 1 cut(s) 168
MnlI CCTC 5 cut(s) 100, 170, 195, 252, 255
MspA1I CMGCKG 2 cut(s) 71, 143
Mva1269I GAATGC 1 cut(s) 219
MwoI GCNNNNNNNGC 1 cut(s) 140
NdeII GATC 2 cut(s) 60, 79
NlaIII CATG 1 cut(s) 350
NspI RCATGY 1 cut(s) 350
PaeI GCATGC 1 cut(s) 350
PceI AGGCCT 1 cut(s) 7
PctI GAATGC 1 cut(s) 219
PkrI GCNGC 2 cut(s) 73, 142
PsuI RGATCY 1 cut(s) 60
PvuII CAGCTG 2 cut(s) 71, 143
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
SatI GCNGC 2 cut(s) 72, 141
Sau3AI GATC 2 cut(s) 60, 79
SetI ASST 7 cut(s) 51, 73, 145, 168, 181, 244, 443
SfaNI GCATC 3 cut(s) 26, 306, 377
SfcI CTRYAG 1 cut(s) 318
SphI GCATGC 1 cut(s) 350
Sse9I AATT 1 cut(s) 168
SseBI AGGCCT 1 cut(s) 7
SspMI CTAG 1 cut(s) 56
StuI AGGCCT 1 cut(s) 7
TaaI ACNGT 2 cut(s) 306, 428
TaqI TCGA 1 cut(s) 247
TasI AATT 1 cut(s) 168
TscAI CASTG 1 cut(s) 311
TseI GCWGC 2 cut(s) 71, 140
TspDTI ATGAA 2 cut(s) 139, 446
TspRI CASTG 1 cut(s) 311
XceI RCATGY 1 cut(s) 350
XspI CTAG 1 cut(s) 56
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.