FvH4_2g14130

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000508
Physical Location & Seq
Reverse (-)
12422578 .. 12424108
1531 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14130.t1

Sequence Viewer

Length: 1056 bp
ATGGCCTCACTCATCGCCTCAGCTCCATGGAAGTACGACGTCTTCTTGAGCAGCTCAAGCCAGGACGCAGGCAACTTTTCATATCTTCTACACAAGGCTTTGAAGGATGAAGGAATTCTCACTCTCAGAGACAGCCAAGAGGTTGAGGGAGCAGAAAGCACAGCTTCCCTGGAGGTCGGAATCAGACTCGAAGGTCGCCCTAGTTATTATCTCGCCGGACTTCCTCCCCTCGGCTCTTGGAACAACTTGTGCAGATTCTTGAAGCAAACAGGGATTCAAGTAGTCTCATCTTCCCAGGCTTTCACCTTGTCGACCCCTCAGATGTCCGAAAACAAAAAGGAACTATTGGAGCAAGCTTCGCCAAGCATGAACAAGAACAAGACTCGGACAAGGTTAAACGGATCAAACAGCGCTTACAATGATATCGTGCAATCTTTAAAACGATGTATCCTCAGGAAGGAAACGGAGAATATTATTGAGCAATCTGTAGACGAGGTCAGCACCAATACCAGTGTTGAGGAGAAGATTATTGAGCAACCAGTAGACGAGGTCAGCAGGAATACCAGTGTCAAGGACAAGATCATCACCGTTAACCATGAGGAGCAAAAGGCCACAGTGGATGCTACTTCATCAGCAGAATGGAACTTCTTACTCTTTACCCTCATCCTCGTGATTCTTTCTGCTGGTTTCGATCAAGCTTCTTCGCCAAGCAAACCGCACTATGCATTAATTGCAATGCTGCTTGCTGCTGCAGCTGTATTCACTTGCATTTGGGAGCTGATTCAAAGGAAAAGCCTAGTACTGAAGAGCTGGAAAAAACTACACTGTATATCTTATCCACATCTTTCGGACACTTTCTTCGAAAGTCACTTAGACACATACGGATTAGTCAGTAGCATCTATCAATGCGTTTTCTCCACAGTTCAGTATATTTACTTCTTTTACAATGCTAATAGTCCTATCAGATTATGCGCCTTACCTGCCCTCTATCTCATGTGTTTGGCTGGTTCAAGACTATGGAAGAAGTACCATAAGAGTAAACCACTACCTGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

352

Amino Acids

39.32

Weight (kDa)

7.58

Isoelectric Point (pI)

49.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 192
AatII GACGTC 1 cut(s) 42
Acc36I ACCTGC 1 cut(s) 988
AccI GTMKAC 3 cut(s) 311, 489, 543
AciI CCGC 1 cut(s) 716
AclWI GGATC 1 cut(s) 409
AcsI RAATTY 1 cut(s) 114
AcuI CTGAAG 1 cut(s) 824
AcyI GRCGYC 1 cut(s) 39
AfaI GTAC 3 cut(s) 35, 801, 1028
AfeI AGCGCT 1 cut(s) 412
AfiI CCNNNNNNNGG 2 cut(s) 230, 457
AgsI TTSAA 5 cut(s) 103, 262, 278, 785, 1011
AjnI CCWGG 3 cut(s) 60, 168, 294
AluBI AGCT 8 cut(s) 23, 54, 164, 356, 698, 755, 778, 810
AluI AGCT 8 cut(s) 23, 54, 164, 356, 698, 755, 778, 810
Alw26I GTCTC 2 cut(s) 123, 289
AlwI GGATC 1 cut(s) 409
Aor51HI AGCGCT 1 cut(s) 412
AoxI GGCC 2 cut(s) 3, 609
ApeKI GCWGC 5 cut(s) 51, 739, 746, 749, 752
ApoI RAATTY 1 cut(s) 114
ArsI GACNNNNNNTTYG 2 cut(s) 842, 874
AseI ATTAAT 1 cut(s) 728
Asp700I GAANNNNTTC 1 cut(s) 114
AspLEI GCGC 2 cut(s) 413, 974
AsuHPI GGTGA 2 cut(s) 295, 577
AsuII TTCGAA 1 cut(s) 861
AxyI CCTNAGG 1 cut(s) 452
BarI GAAGNNNNNNTAC 4 cut(s) 26, 58, 920, 952
BauI CACGAG 1 cut(s) 668
BbsI GAAGAC 1 cut(s) 34
BbvCI CCTCAGC 1 cut(s) 19
BbvI GCAGC 5 cut(s) 63, 726, 733, 736, 764
BciT130I CCWGG 3 cut(s) 62, 170, 296
BciVI GTATCC 1 cut(s) 458
BcoDI GTCTC 2 cut(s) 123, 289
BfaI CTAG 3 cut(s) 201, 797, 1054
BfmI CTRYAG 2 cut(s) 486, 750
BfoI RGCGCY 1 cut(s) 414
BfuAI ACCTGC 1 cut(s) 988
BfuI GTATCC 1 cut(s) 458
BisI GCNGC 5 cut(s) 52, 740, 747, 750, 753
BlsI GCNGC 5 cut(s) 53, 741, 748, 751, 754
BmcAI AGTACT 1 cut(s) 801
Bme1390I CCNGG 3 cut(s) 62, 170, 296
BmrFI CCNGG 3 cut(s) 62, 170, 296
BmsI GCATC 2 cut(s) 610, 906
BpiI GAAGAC 1 cut(s) 34
BpmI CTGGAG 1 cut(s) 191
Bpu10I CCTNAGC 1 cut(s) 19
Bpu14I TTCGAA 1 cut(s) 861
BpuEI CTTGAG 2 cut(s) 40, 67
BsaHI GRCGYC 1 cut(s) 39
BsaJI CCNNGG 4 cut(s) 26, 168, 229, 294
Bsc4I CCNNNNNNNGG 2 cut(s) 230, 457
Bse1I ACTGG 3 cut(s) 510, 539, 564
Bse21I CCTNAGG 1 cut(s) 452
Bse3DI GCAATG 1 cut(s) 741
BseBI CCWGG 3 cut(s) 62, 170, 296
BseDI CCNNGG 4 cut(s) 26, 168, 229, 294
BseGI GGATG 3 cut(s) 112, 625, 663
BseLI CCNNNNNNNGG 2 cut(s) 230, 457
BseMI GCAATG 1 cut(s) 741
BseMII CTCAG 4 cut(s) 33, 139, 332, 466
BseNI ACTGG 3 cut(s) 510, 539, 564
BseRI GAGGAG 2 cut(s) 533, 614
BseXI GCAGC 5 cut(s) 63, 726, 733, 736, 764
BsgI GTGCAG 1 cut(s) 271
BshFI GGCC 2 cut(s) 5, 611
BsiSI CCGG 1 cut(s) 216
BslI CCNNNNNNNGG 2 cut(s) 230, 457
BsmAI GTCTC 2 cut(s) 123, 289
BsnI GGCC 2 cut(s) 5, 611
Bsp119I TTCGAA 1 cut(s) 861
Bsp143I GATC 3 cut(s) 401, 579, 691
Bsp19I CCATGG 1 cut(s) 26
BspACI CCGC 1 cut(s) 716
BspANI GGCC 2 cut(s) 5, 611
BspCNI CTCAG 4 cut(s) 32, 138, 331, 465
BspMAI CTGCAG 1 cut(s) 754
BspMI ACCTGC 1 cut(s) 988
BspPI GGATC 1 cut(s) 409
BspQI GCTCTTC 1 cut(s) 800
BspT104I TTCGAA 1 cut(s) 861
BsrDI GCAATG 1 cut(s) 741
BsrI ACTGG 3 cut(s) 510, 539, 564
BssECI CCNNGG 4 cut(s) 26, 168, 229, 294
BssMI GATC 3 cut(s) 401, 579, 691
BssNI GRCGYC 1 cut(s) 39
BssSI CACGAG 1 cut(s) 668
BssT1I CCWWGG 1 cut(s) 26
Bst2BI CACGAG 1 cut(s) 668
Bst2UI CCWGG 3 cut(s) 62, 170, 296
Bst4CI ACNGT 4 cut(s) 589, 616, 827, 922
Bst6I CTCTTC 1 cut(s) 800
BstACI GRCGYC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 731
BstBI TTCGAA 1 cut(s) 861
BstC8I GCNNGC 3 cut(s) 70, 354, 744
BstDEI CTNAG 5 cut(s) 19, 125, 318, 452, 871
BstDSI CCRYGG 1 cut(s) 26
BstENI CCTNNNNNAGG 1 cut(s) 455
BstF5I GGATG 3 cut(s) 112, 625, 663
BstH2I RGCGCY 1 cut(s) 414
BstHHI GCGC 2 cut(s) 413, 974
BstKTI GATC 3 cut(s) 404, 582, 694
BstMAI GTCTC 2 cut(s) 123, 289
BstMBI GATC 3 cut(s) 401, 579, 691
BstMWI GCNNNNNNNGC 5 cut(s) 57, 358, 731, 752, 980
BstNI CCWGG 3 cut(s) 62, 170, 296
BstSCI CCNGG 3 cut(s) 60, 168, 294
BstSFI CTRYAG 2 cut(s) 486, 750
BstV1I GCAGC 5 cut(s) 63, 726, 733, 736, 764
BstV2I GAAGAC 1 cut(s) 34
Bsu36I CCTNAGG 1 cut(s) 452
BsuI GTATCC 1 cut(s) 458
BsuRI GGCC 2 cut(s) 5, 611
BtgI CCRYGG 1 cut(s) 26
BtsCI GGATG 3 cut(s) 112, 625, 663
BtsIMutI CAGTG 4 cut(s) 517, 571, 621, 823
BveI ACCTGC 1 cut(s) 988
Cac8I GCNNGC 3 cut(s) 70, 354, 744
CfoI GCGC 2 cut(s) 413, 974
CseI GACGC 1 cut(s) 74
Csp6I GTAC 3 cut(s) 34, 800, 1027
CviAII CATG 4 cut(s) 27, 367, 596, 994
CviQI GTAC 3 cut(s) 34, 800, 1027
DdeI CTNAG 5 cut(s) 19, 125, 318, 452, 871
DpnI GATC 3 cut(s) 403, 581, 693
DpnII GATC 3 cut(s) 401, 579, 691
DraI TTTAAA 1 cut(s) 438
DrdI GACNNNNNNGTC 1 cut(s) 192
DseDI GACNNNNNNGTC 1 cut(s) 192
Eam1104I CTCTTC 1 cut(s) 800
EarI CTCTTC 1 cut(s) 800
Eco130I CCWWGG 1 cut(s) 26
Eco32I GATATC 1 cut(s) 424
Eco47III AGCGCT 1 cut(s) 412
Eco57I CTGAAG 1 cut(s) 824
Eco81I CCTNAGG 1 cut(s) 452
EcoNI CCTNNNNNAGG 1 cut(s) 455
EcoRI GAATTC 1 cut(s) 114
EcoRII CCWGG 3 cut(s) 60, 168, 294
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 1 cut(s) 26
EcoT22I ATGCAT 1 cut(s) 727
ErhI CCWWGG 1 cut(s) 26
FaeI CATG 4 cut(s) 30, 370, 599, 997
FalI AAGNNNNNCTT 2 cut(s) 148, 180
FatI CATG 4 cut(s) 26, 366, 595, 993
FblI GTMKAC 3 cut(s) 311, 489, 543
Fnu4HI GCNGC 5 cut(s) 52, 740, 747, 750, 753
FokI GGATG 3 cut(s) 119, 632, 650
Fsp4HI GCNGC 5 cut(s) 52, 740, 747, 750, 753
FspBI CTAG 3 cut(s) 201, 797, 1054
GlaI GCGC 2 cut(s) 412, 973
GluI GCNGC 5 cut(s) 52, 740, 747, 750, 753
GsuI CTGGAG 1 cut(s) 191
HaeII RGCGCY 1 cut(s) 414
HaeIII GGCC 2 cut(s) 5, 611
HapII CCGG 1 cut(s) 216
HgaI GACGC 1 cut(s) 74
HhaI GCGC 2 cut(s) 413, 974
Hin1I GRCGYC 1 cut(s) 39
Hin1II CATG 4 cut(s) 30, 370, 599, 997
Hin6I GCGC 2 cut(s) 411, 972
HinP1I GCGC 2 cut(s) 411, 972
HincII GTYRAC 2 cut(s) 312, 592
HindII GTYRAC 2 cut(s) 312, 592
HindIII AAGCTT 2 cut(s) 354, 696
HinfI GANTC 7 cut(s) 180, 186, 255, 274, 382, 673, 781
HpaI GTTAAC 1 cut(s) 592
HpaII CCGG 1 cut(s) 216
HphI GGTGA 2 cut(s) 295, 577
Hpy166II GTNNAC 5 cut(s) 312, 490, 544, 592, 1040
Hpy188I TCNGA 8 cut(s) 128, 179, 185, 321, 328, 387, 850, 965
Hpy188III TCNNGA 5 cut(s) 46, 259, 454, 670, 1011
Hpy8I GTNNAC 5 cut(s) 312, 490, 544, 592, 1040
Hpy99I CGWCG 1 cut(s) 41
HpyAV CCTTC 4 cut(s) 97, 104, 185, 451
HpyCH4III ACNGT 4 cut(s) 589, 616, 827, 922
HpyCH4IV ACGT 1 cut(s) 39
HpyCH4V TGCA 6 cut(s) 252, 430, 725, 734, 752, 768
HpyF10VI GCNNNNNNNGC 5 cut(s) 57, 358, 731, 752, 980
HpyF3I CTNAG 5 cut(s) 19, 125, 318, 452, 871
HpySE526I ACGT 1 cut(s) 39
Hsp92I GRCGYC 1 cut(s) 39
Hsp92II CATG 4 cut(s) 30, 370, 599, 997
HspAI GCGC 2 cut(s) 411, 972
KspAI GTTAAC 1 cut(s) 592
Kzo9I GATC 3 cut(s) 401, 579, 691
LguI GCTCTTC 1 cut(s) 800
LmnI GCTCC 5 cut(s) 28, 149, 349, 601, 775
Lsp1109I GCAGC 5 cut(s) 63, 726, 733, 736, 764
LweI GCATC 2 cut(s) 610, 906
MaeI CTAG 3 cut(s) 201, 797, 1054
MaeII ACGT 1 cut(s) 39
MaeIII GTNAC 1 cut(s) 866
MalI GATC 3 cut(s) 403, 581, 693
MboI GATC 3 cut(s) 401, 579, 691
MboII GAAGA 8 cut(s) 34, 77, 282, 535, 693, 817, 850, 1033
MluCI AATT 2 cut(s) 114, 729
MlyI GAGTC 2 cut(s) 180, 376
MmeI TCCRAC 1 cut(s) 157
Mph1103I ATGCAT 1 cut(s) 727
MroXI GAANNNNTTC 1 cut(s) 114
MseI TTAA 4 cut(s) 395, 437, 591, 728
MslI CAYNNNNRTG 1 cut(s) 668
MspA1I CMGCKG 1 cut(s) 755
MspI CCGG 1 cut(s) 216
MspR9I CCNGG 3 cut(s) 62, 170, 296
MvaI CCWGG 3 cut(s) 62, 170, 296
MwoI GCNNNNNNNGC 5 cut(s) 57, 358, 731, 752, 980
NcoI CCATGG 1 cut(s) 26
NdeII GATC 3 cut(s) 401, 579, 691
NlaIII CATG 4 cut(s) 30, 370, 599, 997
NmeAIII GCCGAG 1 cut(s) 210
NmuCI GTSAC 1 cut(s) 866
NsiI ATGCAT 1 cut(s) 727
NspV TTCGAA 1 cut(s) 861
PciSI GCTCTTC 1 cut(s) 800
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 5 cut(s) 180, 255, 274, 673, 781
PflFI GACNNNGTC 2 cut(s) 494, 548
PkrI GCNGC 5 cut(s) 53, 741, 748, 751, 754
PleI GAGTC 2 cut(s) 180, 376
PpsI GAGTC 2 cut(s) 180, 376
PshBI ATTAAT 1 cut(s) 728
Psp6I CCWGG 3 cut(s) 60, 168, 294
PspGI CCWGG 3 cut(s) 60, 168, 294
PstI CTGCAG 1 cut(s) 754
PsyI GACNNNGTC 2 cut(s) 494, 548
PvuII CAGCTG 1 cut(s) 755
RsaI GTAC 3 cut(s) 35, 801, 1028
RsaNI GTAC 3 cut(s) 34, 800, 1027
RseI CAYNNNNRTG 1 cut(s) 668
SalI GTCGAC 1 cut(s) 310
SapI GCTCTTC 1 cut(s) 800
SaqAI TTAA 4 cut(s) 395, 437, 591, 728
SatI GCNGC 5 cut(s) 52, 740, 747, 750, 753
Sau3AI GATC 3 cut(s) 401, 579, 691
ScaI AGTACT 1 cut(s) 801
SchI GAGTC 2 cut(s) 180, 376
ScrFI CCNGG 3 cut(s) 62, 170, 296
SfaNI GCATC 2 cut(s) 610, 906
SfcI CTRYAG 2 cut(s) 486, 750
SfuI TTCGAA 1 cut(s) 861
SmiMI CAYNNNNRTG 1 cut(s) 668
SmlI CTYRAG 2 cut(s) 46, 55
SmoI CTYRAG 2 cut(s) 46, 55
Sse9I AATT 2 cut(s) 114, 729
SsiI CCGC 1 cut(s) 716
SspI AATATT 1 cut(s) 472
SspMI CTAG 3 cut(s) 201, 797, 1054
StyD4I CCNGG 3 cut(s) 60, 168, 294
StyI CCWWGG 1 cut(s) 26
TaaI ACNGT 4 cut(s) 589, 616, 827, 922
TaiI ACGT 1 cut(s) 42
TaqI TCGA 4 cut(s) 189, 311, 690, 861
TasI AATT 2 cut(s) 114, 729
TatI WGTACW 1 cut(s) 799
TfiI GAWTC 5 cut(s) 180, 255, 274, 673, 781
Tru1I TTAA 4 cut(s) 395, 437, 591, 728
Tru9I TTAA 4 cut(s) 395, 437, 591, 728
TscAI CASTG 4 cut(s) 517, 571, 621, 830
TseFI GTSAC 1 cut(s) 866
TseI GCWGC 5 cut(s) 51, 739, 746, 749, 752
Tsp45I GTSAC 1 cut(s) 866
TspDTI ATGAA 4 cut(s) 69, 123, 383, 618
TspGWI ACGGA 3 cut(s) 414, 479, 897
TspRI CASTG 4 cut(s) 517, 571, 621, 830
Tth111I GACNNNGTC 2 cut(s) 494, 548
VspI ATTAAT 1 cut(s) 728
XagI CCTNNNNNAGG 1 cut(s) 455
XapI RAATTY 1 cut(s) 114
XmiI GTMKAC 3 cut(s) 311, 489, 543
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 3 cut(s) 201, 797, 1054
ZraI GACGTC 1 cut(s) 40
ZrmI AGTACT 1 cut(s) 801
Zsp2I ATGCAT 1 cut(s) 727
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.