Rorug06G0099400

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0000508
Physical Location & Seq
Forward (+)
13241138 .. 13242059
922 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0099400.1

Sequence Viewer

Length: 768 bp
ATGTTGGAGGTGGGTTTGATGCATTTTGTGGGTTGCGTCAGCAAAGATCCAGTTCCTCGTCCGGTCGGCCGGAGACTGGATGAAGACTCGGTCGCCGCGGGTGAGTGTGGCTCATCCACACTGAAGGTGAGTGTGAGCCTAGCACTCCCCTCTAAAAAATATGATGCTCACTTTGATGAAGCCCTAAATGGGCGCTGTATATTTCTTGTTGGTATGATGGGCTCTGGGAAAACAACTGTTGGCAAGATTTTATCAGAAACACTTCATGATTATTCTTTTGTTGATATTGATAAATTGGGGGAGGATGAGGTTGGTGGAAAATCTGTAGCTGAAATTTTCAAGCTGTATGGGGAGGGATTCTTTAGAGACAAGGAGACCGAGGTTCTGCATAAGTTATCTTTGAGTCAACGTCTGGTTGTCTCTACTGGTGGAGGTGTGGTGGTTCGGCCGGTGAACTGGAAATATATGCAGAAGGGGATTAGTGTGTGGTTAGATGTACCCTTGGAGGCCTTAGCTCAGAGACTCGCAGGTGCCGGAACTGATTCGCGGCCCCTTTTGCATCACAGAGAAACCATCGTGCGTCTGAGAAGTCTTTTTGAAGAGAGGGGGGATGCATATGCCAATGCCGATGCTAGGATTTCGTTGGAAAATGTTGCTGCCAAAATGGGGTGTAGAGATGTATCCAATATCACACCTAGTGTTATTGCTTTCGAGGCGCTTGAACAACTTCAAGGCTTTCTCAAGGAAGAGGAAGGCCGTTCATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

255

Amino Acids

27.9

Weight (kDa)

5.75

Isoelectric Point (pI)

38.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_18 PF13238 67 - 181 3e-09 AAA domain
SKI PF01202 73 - 220 8.9e-41 Shikimate kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 518
Acc36I ACCTGC 1 cut(s) 518
AccB1I GGYRCC 1 cut(s) 530
AccII CGCG 2 cut(s) 98, 547
AciI CCGC 3 cut(s) 96, 98, 547
AclWI GGATC 1 cut(s) 41
AcoI YGGCCR 2 cut(s) 67, 446
AcsI RAATTY 1 cut(s) 333
AcuI CTGAAG 1 cut(s) 143
AdeI CACNNNGTG 1 cut(s) 698
AfaI GTAC 1 cut(s) 498
AfiI CCNNNNNNNGG 4 cut(s) 76, 189, 633, 666
AgsI TTSAA 4 cut(s) 340, 599, 722, 731
AluBI AGCT 3 cut(s) 329, 343, 515
AluI AGCT 3 cut(s) 329, 343, 515
Alw26I GTCTC 5 cut(s) 67, 360, 368, 424, 514
AlwI GGATC 1 cut(s) 41
AoxI GGCC 5 cut(s) 67, 446, 507, 548, 754
ApeKI GCWGC 1 cut(s) 656
ApoI RAATTY 1 cut(s) 333
Asp700I GAANNNNTTC 3 cut(s) 261, 541, 726
AspLEI GCGC 2 cut(s) 195, 718
AspS9I GGNCC 1 cut(s) 549
AsuHPI GGTGA 3 cut(s) 113, 139, 463
BanI GGYRCC 1 cut(s) 530
BanII GRGCYC 1 cut(s) 224
BbsI GAAGAC 1 cut(s) 90
BbvI GCAGC 1 cut(s) 643
BccI CCATC 2 cut(s) 211, 581
BceAI ACGGC 1 cut(s) 741
BciVI GTATCC 1 cut(s) 691
BcoDI GTCTC 5 cut(s) 67, 360, 368, 424, 514
BfaI CTAG 3 cut(s) 140, 633, 696
BfmI CTRYAG 1 cut(s) 324
BfoI RGCGCY 2 cut(s) 196, 719
BfuAI ACCTGC 1 cut(s) 518
BfuI GTATCC 1 cut(s) 691
BisI GCNGC 3 cut(s) 96, 548, 657
BlsI GCNGC 3 cut(s) 97, 549, 658
BmgT120I GGNCC 1 cut(s) 549
BmiI GGNNCC 2 cut(s) 532, 551
BmsI GCATC 5 cut(s) 9, 154, 568, 601, 619
BpiI GAAGAC 1 cut(s) 90
BplI GAGNNNNNCTC 2 cut(s) 95, 127
Bpu10I CCTNAGC 1 cut(s) 511
BpuEI CTTGAG 1 cut(s) 725
BsaI GGTCTC 1 cut(s) 368
BsaJI CCNNGG 3 cut(s) 96, 378, 501
BsaWI WCCGGW 1 cut(s) 61
BsaXI ACNNNNNCTCC 2 cut(s) 423, 453
Bsc4I CCNNNNNNNGG 4 cut(s) 76, 189, 633, 666
Bse118I RCCGGY 1 cut(s) 448
Bse1I ACTGG 4 cut(s) 50, 81, 430, 461
BseDI CCNNGG 3 cut(s) 96, 378, 501
BseGI GGATG 4 cut(s) 85, 113, 310, 616
BseLI CCNNNNNNNGG 4 cut(s) 76, 189, 633, 666
BseMII CTCAG 2 cut(s) 530, 575
BseNI ACTGG 4 cut(s) 50, 81, 430, 461
BseX3I CGGCCG 2 cut(s) 67, 446
BseXI GCAGC 1 cut(s) 643
Bsh1236I CGCG 2 cut(s) 98, 547
Bsh1285I CGRYCG 4 cut(s) 66, 70, 93, 449
BshFI GGCC 5 cut(s) 69, 448, 509, 550, 756
BshNI GGYRCC 1 cut(s) 530
BsiEI CGRYCG 4 cut(s) 66, 70, 93, 449
BsiSI CCGG 4 cut(s) 62, 70, 449, 534
BslI CCNNNNNNNGG 4 cut(s) 76, 189, 633, 666
BsmAI GTCTC 5 cut(s) 67, 360, 368, 424, 514
BsnI GGCC 5 cut(s) 69, 448, 509, 550, 756
Bso31I GGTCTC 1 cut(s) 368
Bsp1286I GDGCHC 1 cut(s) 224
Bsp143I GATC 1 cut(s) 46
BspACI CCGC 3 cut(s) 96, 98, 547
BspANI GGCC 5 cut(s) 69, 448, 509, 550, 756
BspCNI CTCAG 2 cut(s) 529, 576
BspFNI CGCG 2 cut(s) 98, 547
BspHI TCATGA 1 cut(s) 265
BspLI GGNNCC 2 cut(s) 532, 551
BspMI ACCTGC 1 cut(s) 518
BspPI GGATC 1 cut(s) 41
BspT107I GGYRCC 1 cut(s) 530
BspTNI GGTCTC 1 cut(s) 368
BsrFI RCCGGY 1 cut(s) 448
BsrI ACTGG 4 cut(s) 50, 81, 430, 461
BssAI RCCGGY 1 cut(s) 448
BssECI CCNNGG 3 cut(s) 96, 378, 501
BssMI GATC 1 cut(s) 46
BssT1I CCWWGG 1 cut(s) 501
Bst4CI ACNGT 1 cut(s) 238
Bst6I CTCTTC 2 cut(s) 594, 741
BstDEI CTNAG 3 cut(s) 511, 516, 584
BstDSI CCRYGG 1 cut(s) 96
BstF5I GGATG 4 cut(s) 85, 113, 310, 616
BstFNI CGCG 2 cut(s) 98, 547
BstH2I RGCGCY 2 cut(s) 196, 719
BstHHI GCGC 2 cut(s) 195, 718
BstKTI GATC 1 cut(s) 49
BstMAI GTCTC 5 cut(s) 67, 360, 368, 424, 514
BstMBI GATC 1 cut(s) 46
BstMCI CGRYCG 4 cut(s) 66, 70, 93, 449
BstMWI GCNNNNNNNGC 2 cut(s) 556, 713
BstSFI CTRYAG 1 cut(s) 324
BstUI CGCG 2 cut(s) 98, 547
BstV1I GCAGC 1 cut(s) 643
BstV2I GAAGAC 1 cut(s) 90
BstX2I RGATCY 1 cut(s) 46
BstYI RGATCY 1 cut(s) 46
BstZI CGGCCG 2 cut(s) 67, 446
BsuI GTATCC 1 cut(s) 691
BsuRI GGCC 5 cut(s) 69, 448, 509, 550, 756
BtgI CCRYGG 1 cut(s) 96
BtsCI GGATG 4 cut(s) 85, 113, 310, 616
BtsIMutI CAGTG 1 cut(s) 119
BveI ACCTGC 1 cut(s) 518
CciI TCATGA 1 cut(s) 265
CfoI GCGC 2 cut(s) 195, 718
Cfr10I RCCGGY 1 cut(s) 448
Cfr13I GGNCC 1 cut(s) 549
Cfr42I CCGCGG 1 cut(s) 99
CseI GACGC 2 cut(s) 25, 569
Csp6I GTAC 1 cut(s) 497
CviAII CATG 1 cut(s) 266
CviQI GTAC 1 cut(s) 497
DdeI CTNAG 3 cut(s) 511, 516, 584
DpnI GATC 1 cut(s) 48
DpnII GATC 1 cut(s) 46
DraIII CACNNNGTG 1 cut(s) 698
EaeI YGGCCR 2 cut(s) 67, 446
EagI CGGCCG 2 cut(s) 67, 446
Eam1104I CTCTTC 2 cut(s) 594, 741
EarI CTCTTC 2 cut(s) 594, 741
EclXI CGGCCG 2 cut(s) 67, 446
Eco130I CCWWGG 1 cut(s) 501
Eco147I AGGCCT 1 cut(s) 509
Eco24I GRGCYC 1 cut(s) 224
Eco31I GGTCTC 1 cut(s) 368
Eco52I CGGCCG 2 cut(s) 67, 446
Eco57I CTGAAG 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 501
EcoT22I ATGCAT 2 cut(s) 24, 616
EcoT38I GRGCYC 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 1 cut(s) 269
FatI CATG 1 cut(s) 265
FauI CCCGC 1 cut(s) 91
FauNDI CATATG 1 cut(s) 616
Fnu4HI GCNGC 3 cut(s) 96, 548, 657
FokI GGATG 4 cut(s) 92, 100, 317, 623
FriOI GRGCYC 1 cut(s) 224
Fsp4HI GCNGC 3 cut(s) 96, 548, 657
FspBI CTAG 3 cut(s) 140, 633, 696
GlaI GCGC 2 cut(s) 194, 717
GluI GCNGC 3 cut(s) 96, 548, 657
HaeII RGCGCY 2 cut(s) 196, 719
HaeIII GGCC 5 cut(s) 69, 448, 509, 550, 756
HapII CCGG 4 cut(s) 62, 70, 449, 534
HgaI GACGC 2 cut(s) 25, 569
HhaI GCGC 2 cut(s) 195, 718
Hin1II CATG 1 cut(s) 269
Hin6I GCGC 2 cut(s) 193, 716
HinP1I GCGC 2 cut(s) 193, 716
HincII GTYRAC 1 cut(s) 407
HindII GTYRAC 1 cut(s) 407
HinfI GANTC 5 cut(s) 86, 357, 403, 522, 542
HpaII CCGG 4 cut(s) 62, 70, 449, 534
HphI GGTGA 3 cut(s) 113, 139, 463
Hpy166II GTNNAC 2 cut(s) 407, 454
Hpy188I TCNGA 3 cut(s) 256, 519, 585
Hpy188III TCNNGA 1 cut(s) 266
Hpy8I GTNNAC 2 cut(s) 407, 454
HpyAV CCTTC 3 cut(s) 118, 466, 746
HpyCH4III ACNGT 1 cut(s) 238
HpyCH4IV ACGT 1 cut(s) 409
HpyCH4V TGCA 5 cut(s) 22, 388, 469, 559, 614
HpyF10VI GCNNNNNNNGC 2 cut(s) 556, 713
HpyF3I CTNAG 3 cut(s) 511, 516, 584
HpySE526I ACGT 1 cut(s) 409
Hsp92II CATG 1 cut(s) 269
HspAI GCGC 2 cut(s) 193, 716
KspI CCGCGG 1 cut(s) 99
Kzo9I GATC 1 cut(s) 46
Lsp1109I GCAGC 1 cut(s) 643
LweI GCATC 5 cut(s) 9, 154, 568, 601, 619
MaeI CTAG 3 cut(s) 140, 633, 696
MaeII ACGT 1 cut(s) 409
MalI GATC 1 cut(s) 48
MboI GATC 1 cut(s) 46
MboII GAAGA 3 cut(s) 95, 611, 758
MflI RGATCY 1 cut(s) 46
MhlI GDGCHC 1 cut(s) 224
MluCI AATT 2 cut(s) 293, 333
MlyI GAGTC 3 cut(s) 80, 412, 516
MmeI TCCRAC 1 cut(s) 624
Mph1103I ATGCAT 2 cut(s) 24, 616
MroXI GAANNNNTTC 3 cut(s) 261, 541, 726
MslI CAYNNNNRTG 1 cut(s) 174
MspA1I CMGCKG 1 cut(s) 98
MspI CCGG 4 cut(s) 62, 70, 449, 534
MvnI CGCG 2 cut(s) 98, 547
MwoI GCNNNNNNNGC 2 cut(s) 556, 713
NdeI CATATG 1 cut(s) 616
NdeII GATC 1 cut(s) 46
NlaIII CATG 1 cut(s) 269
NlaIV GGNNCC 2 cut(s) 532, 551
NsiI ATGCAT 2 cut(s) 24, 616
PagI TCATGA 1 cut(s) 265
PaqCI CACCTGC 1 cut(s) 518
PceI AGGCCT 1 cut(s) 509
PdmI GAANNNNTTC 3 cut(s) 261, 541, 726
PfeI GAWTC 2 cut(s) 357, 542
PflFI GACNNNGTC 1 cut(s) 89
PkrI GCNGC 3 cut(s) 97, 549, 658
PleI GAGTC 3 cut(s) 80, 411, 516
PpsI GAGTC 3 cut(s) 80, 411, 516
PspN4I GGNNCC 2 cut(s) 532, 551
PspPI GGNCC 1 cut(s) 549
PsuI RGATCY 1 cut(s) 46
PsyI GACNNNGTC 1 cut(s) 89
RsaI GTAC 1 cut(s) 498
RsaNI GTAC 1 cut(s) 497
RseI CAYNNNNRTG 1 cut(s) 174
SacII CCGCGG 1 cut(s) 99
SatI GCNGC 3 cut(s) 96, 548, 657
Sau3AI GATC 1 cut(s) 46
Sau96I GGNCC 1 cut(s) 549
SchI GAGTC 3 cut(s) 80, 412, 516
SduI GDGCHC 1 cut(s) 224
SfaNI GCATC 5 cut(s) 9, 154, 568, 601, 619
SfcI CTRYAG 1 cut(s) 324
Sfr303I CCGCGG 1 cut(s) 99
SgrBI CCGCGG 1 cut(s) 99
SmiMI CAYNNNNRTG 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 740
SmoI CTYRAG 1 cut(s) 740
Sse9I AATT 2 cut(s) 293, 333
SseBI AGGCCT 1 cut(s) 509
SsiI CCGC 3 cut(s) 96, 98, 547
SspMI CTAG 3 cut(s) 140, 633, 696
StuI AGGCCT 1 cut(s) 509
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 1 cut(s) 238
TaiI ACGT 1 cut(s) 412
TaqI TCGA 1 cut(s) 711
TaqII GACCGA 2 cut(s) 79, 392
TasI AATT 2 cut(s) 293, 333
TauI GCSGC 2 cut(s) 98, 550
TfiI GAWTC 2 cut(s) 357, 542
TscAI CASTG 1 cut(s) 126
TseI GCWGC 1 cut(s) 656
TspDTI ATGAA 4 cut(s) 96, 192, 254, 750
TspRI CASTG 1 cut(s) 126
Tth111I GACNNNGTC 1 cut(s) 89
XapI RAATTY 1 cut(s) 333
XmnI GAANNNNTTC 3 cut(s) 261, 541, 726
XspI CTAG 3 cut(s) 140, 633, 696
Zsp2I ATGCAT 2 cut(s) 24, 616
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.