FvH4_2g14140

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000508
Physical Location & Seq
Reverse (-)
12425270 .. 12428447
3178 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g14140.t1

Sequence Viewer

Length: 1107 bp
ATGGTTTTGAAGAAGCAGAGAGTATCTATTCCATTTCCATTACGGATATCTATTCCTTCAGCTTCTTTATGGGCGGTGGATGTTTTCTTAAGTTGTGACCATGAAGACATTTTCTGCTCCCACTTGTACCACAAGTTGCAGTCTCGAGGAATTTGTACTTTCCTGGATGGCCATGAATTTGCAAGAAACACAACCCCTCTTGAGCTCTATGGTGCAATCGAAGAATCGAGGTTTGCCATTGTTGTTCTCTCGCAAAACTATGCTTCTTCTCCACGGCGCTTGAATGAGCTTTCCAAAACTCTCGAGTGCATGAAAGATAGGAACAGGATTCTACCGATATTTCATGATGTGAATCTCTTCCATGTCCAAAAACAAAAAGGGACTTTTGAGAAAGCATTTGCCAAGCATGAAGAAATGTTTCAGGATGACTTGGCAAAGGTTGAAGCTTGGAGAGATGCTTTAACCAAAGTATGCAACTTTGCTGGTTGGACTTCCAATGATAGGAATGAAGTACAAGTTCTAGAAGAAATTACTGAAGCAGTCTGGAACAAAATACATCCTATACCAAGTCCAACCGAAAGAGTGCATCCGACATCCACACACAACTTGGTTGCATACACTAGCATTAATGTTAACGAGGGCAACCGTAACCATCCAATGCACACTACAGATTTGGTGGATACTTCAGCAGAGTGGAACTTTTTGATCACTAACCTTGGCCTAGAGATTTTGTCAACCGGTTTTGATCAGGCCTCCTCCTCAAGTAAGCCACAGTATGCACTATTTGGTATGCTGTTTTCTATTGCAGCCTTGTTCACTTGCATTTTGGAGCTCATTTACAAGGGCATAAAGAATAAAGTAGTACTGAAGAAGTTCGGAAAACTCTGGTGGTATTATTATCCACATCCCCACGATAGCGTGCCTTTTGGTACTCTCCCTTACATTTACGGATTATTTGGAGGCGCTGCTCAGTGCATATGCTCTATTACTCAATACATTTACTTCTCTTGGCATGCTGATAGTCCTATAAAACTATCATTTTTACCAGCAATCTTTCTTTTGTGTTTGGCTGGTTCAAGACTACACAGCAATCATAATCATTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_3 NB-ARC TIR

Protein Analysis

369

Amino Acids

42.09

Weight (kDa)

7.0

Isoelectric Point (pI)

45.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 27 - 190 3.9e-35 TIR domain
TIR_2 PF13676 28 - 117 8.5e-12 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 74
AcoI YGGCCR 1 cut(s) 169
AcsI RAATTY 2 cut(s) 150, 176
AcuI CTGAAG 4 cut(s) 42, 555, 669, 887
AfaI GTAC 5 cut(s) 128, 157, 513, 864, 931
AfiI CCNNNNNNNGG 1 cut(s) 501
AflII CTTAAG 1 cut(s) 88
AgeI ACCGGT 1 cut(s) 737
AgsI TTSAA 4 cut(s) 10, 283, 443, 1077
AjnI CCWGG 1 cut(s) 162
AluBI AGCT 5 cut(s) 62, 205, 289, 446, 832
AluI AGCT 5 cut(s) 62, 205, 289, 446, 832
Alw21I GWGCWC 2 cut(s) 207, 834
Alw26I GTCTC 1 cut(s) 147
Ama87I CYCGRG 2 cut(s) 144, 302
AoxI GGCC 3 cut(s) 169, 718, 750
ApeKI GCWGC 2 cut(s) 806, 965
ApoI RAATTY 2 cut(s) 150, 176
AseI ATTAAT 1 cut(s) 627
AsiGI ACCGGT 1 cut(s) 737
Asp700I GAANNNNTTC 3 cut(s) 356, 417, 872
AspLEI GCGC 2 cut(s) 279, 965
AvaI CYCGRG 2 cut(s) 144, 302
BalI TGGCCA 1 cut(s) 171
BanII GRGCYC 2 cut(s) 207, 834
BbsI GAAGAC 1 cut(s) 111
Bbv12I GWGCWC 2 cut(s) 207, 834
BbvI GCAGC 2 cut(s) 818, 952
BccI CCATC 2 cut(s) 161, 660
BceAI ACGGC 1 cut(s) 290
BciT130I CCWGG 1 cut(s) 164
BciVI GTATCC 1 cut(s) 673
BclI TGATCA 2 cut(s) 705, 745
BcoDI GTCTC 1 cut(s) 147
BfaI CTAG 3 cut(s) 521, 621, 722
BfmI CTRYAG 1 cut(s) 666
BfoI RGCGCY 2 cut(s) 280, 966
BfrI CTTAAG 1 cut(s) 88
BfuI GTATCC 1 cut(s) 673
BisI GCNGC 2 cut(s) 807, 966
BlsI GCNGC 2 cut(s) 808, 967
BmcAI AGTACT 1 cut(s) 864
Bme1390I CCNGG 1 cut(s) 164
BmeT110I CYCGRG 2 cut(s) 144, 302
BmrFI CCNGG 1 cut(s) 164
BmsI GCATC 2 cut(s) 445, 595
BpiI GAAGAC 1 cut(s) 111
BpuEI CTTGAG 2 cut(s) 221, 745
BsaBI GATNNNNATC 1 cut(s) 351
BsaJI CCNNGG 2 cut(s) 272, 715
BsaWI WCCGGW 1 cut(s) 737
Bsc4I CCNNNNNNNGG 1 cut(s) 501
Bse118I RCCGGY 1 cut(s) 737
Bse8I GATNNNNATC 1 cut(s) 351
BseBI CCWGG 1 cut(s) 164
BseDI CCNNGG 2 cut(s) 272, 715
BseGI GGATG 8 cut(s) 85, 172, 430, 556, 586, 593, 652, 904
BseJI GATNNNNATC 1 cut(s) 351
BseLI CCNNNNNNNGG 1 cut(s) 501
BseMII CTCAG 1 cut(s) 983
BseRI GAGGAG 2 cut(s) 745, 748
BseXI GCAGC 2 cut(s) 818, 952
BshFI GGCC 3 cut(s) 171, 720, 752
BshTI ACCGGT 1 cut(s) 737
BsiHKAI GWGCWC 2 cut(s) 207, 834
BsiHKCI CYCGRG 2 cut(s) 144, 302
BsiSI CCGG 1 cut(s) 738
BslFI GGGAC 1 cut(s) 394
BslI CCNNNNNNNGG 1 cut(s) 501
BsmAI GTCTC 1 cut(s) 147
BsmFI GGGAC 1 cut(s) 394
BsnI GGCC 3 cut(s) 171, 720, 752
BsoBI CYCGRG 2 cut(s) 144, 302
Bsp1286I GDGCHC 2 cut(s) 207, 834
Bsp143I GATC 2 cut(s) 705, 745
BspACI CCGC 1 cut(s) 74
BspANI GGCC 3 cut(s) 171, 720, 752
BspCNI CTCAG 1 cut(s) 982
BspHI TCATGA 1 cut(s) 343
BspTI CTTAAG 1 cut(s) 88
BsrFI RCCGGY 1 cut(s) 737
BssAI RCCGGY 1 cut(s) 737
BssECI CCNNGG 2 cut(s) 272, 715
BssMI GATC 2 cut(s) 705, 745
BssT1I CCWWGG 1 cut(s) 715
Bst2UI CCWGG 1 cut(s) 164
Bst4CI ACNGT 2 cut(s) 647, 774
Bst6I CTCTTC 1 cut(s) 362
BstAFI CTTAAG 1 cut(s) 88
BstC8I GCNNGC 2 cut(s) 920, 1014
BstDEI CTNAG 1 cut(s) 969
BstDSI CCRYGG 1 cut(s) 272
BstF5I GGATG 8 cut(s) 85, 172, 430, 556, 586, 593, 652, 904
BstH2I RGCGCY 2 cut(s) 280, 966
BstHHI GCGC 2 cut(s) 279, 965
BstKTI GATC 2 cut(s) 708, 748
BstMAI GTCTC 1 cut(s) 147
BstMBI GATC 2 cut(s) 705, 745
BstNI CCWGG 1 cut(s) 164
BstNSI RCATGY 1 cut(s) 1016
BstSCI CCNGG 1 cut(s) 162
BstSFI CTRYAG 1 cut(s) 666
BstV1I GCAGC 2 cut(s) 818, 952
BstV2I GAAGAC 1 cut(s) 111
BsuI GTATCC 1 cut(s) 673
BsuRI GGCC 3 cut(s) 171, 720, 752
BtgI CCRYGG 1 cut(s) 272
BtsCI GGATG 8 cut(s) 85, 172, 430, 556, 586, 593, 652, 904
BtsIMutI CAGTG 1 cut(s) 977
Cac8I GCNNGC 2 cut(s) 920, 1014
CciI TCATGA 1 cut(s) 343
CfoI GCGC 2 cut(s) 279, 965
Cfr10I RCCGGY 1 cut(s) 737
Csp6I GTAC 5 cut(s) 127, 156, 512, 863, 930
CspAI ACCGGT 1 cut(s) 737
CspCI CAANNNNNGTGG 2 cut(s) 261, 296
CviAII CATG 7 cut(s) 101, 173, 310, 344, 362, 407, 1013
CviQI GTAC 5 cut(s) 127, 156, 512, 863, 930
DdeI CTNAG 1 cut(s) 969
DpnI GATC 2 cut(s) 707, 747
DpnII GATC 2 cut(s) 705, 745
EaeI YGGCCR 1 cut(s) 169
Eam1104I CTCTTC 1 cut(s) 362
EarI CTCTTC 1 cut(s) 362
Ecl136II GAGCTC 2 cut(s) 205, 832
Eco130I CCWWGG 1 cut(s) 715
Eco147I AGGCCT 1 cut(s) 752
Eco24I GRGCYC 2 cut(s) 207, 834
Eco32I GATATC 1 cut(s) 48
Eco53kI GAGCTC 2 cut(s) 205, 832
Eco57I CTGAAG 4 cut(s) 42, 555, 669, 887
Eco88I CYCGRG 2 cut(s) 144, 302
EcoICRI GAGCTC 2 cut(s) 205, 832
EcoRII CCWGG 1 cut(s) 162
EcoRV GATATC 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 715
EcoT38I GRGCYC 2 cut(s) 207, 834
ErhI CCWWGG 1 cut(s) 715
FaeI CATG 7 cut(s) 104, 176, 313, 347, 365, 410, 1016
FaqI GGGAC 1 cut(s) 394
FatI CATG 7 cut(s) 100, 172, 309, 343, 361, 406, 1012
FauNDI CATATG 1 cut(s) 977
FbaI TGATCA 2 cut(s) 705, 745
Fnu4HI GCNGC 2 cut(s) 807, 966
FokI GGATG 8 cut(s) 92, 179, 437, 543, 573, 580, 639, 891
FriOI GRGCYC 2 cut(s) 207, 834
Fsp4HI GCNGC 2 cut(s) 807, 966
FspBI CTAG 3 cut(s) 521, 621, 722
GlaI GCGC 2 cut(s) 278, 964
GluI GCNGC 2 cut(s) 807, 966
HaeII RGCGCY 2 cut(s) 280, 966
HaeIII GGCC 3 cut(s) 171, 720, 752
HapII CCGG 1 cut(s) 738
HhaI GCGC 2 cut(s) 279, 965
Hin1II CATG 7 cut(s) 104, 176, 313, 347, 365, 410, 1016
Hin6I GCGC 2 cut(s) 277, 963
HinP1I GCGC 2 cut(s) 277, 963
HincII GTYRAC 2 cut(s) 634, 735
HindII GTYRAC 2 cut(s) 634, 735
HindIII AAGCTT 1 cut(s) 444
HinfI GANTC 3 cut(s) 224, 328, 352
HpaI GTTAAC 1 cut(s) 634
HpaII CCGG 1 cut(s) 738
Hpy166II GTNNAC 3 cut(s) 634, 735, 816
Hpy188I TCNGA 2 cut(s) 591, 878
Hpy188III TCNNGA 8 cut(s) 144, 200, 302, 344, 422, 521, 544, 1077
Hpy8I GTNNAC 3 cut(s) 634, 735, 816
HpyAV CCTTC 1 cut(s) 66
HpyCH4III ACNGT 2 cut(s) 647, 774
HpyF3I CTNAG 1 cut(s) 969
Hsp92II CATG 7 cut(s) 104, 176, 313, 347, 365, 410, 1016
HspAI GCGC 2 cut(s) 277, 963
Ksp22I TGATCA 2 cut(s) 705, 745
KspAI GTTAAC 1 cut(s) 634
Kzo9I GATC 2 cut(s) 705, 745
LmnI GCTCC 2 cut(s) 122, 829
Lsp1109I GCAGC 2 cut(s) 818, 952
LweI GCATC 2 cut(s) 445, 595
MaeI CTAG 3 cut(s) 521, 621, 722
MaeIII GTNAC 2 cut(s) 95, 647
MalI GATC 2 cut(s) 707, 747
MboI GATC 2 cut(s) 705, 745
MboII GAAGA 8 cut(s) 22, 116, 233, 258, 349, 422, 536, 880
MhlI GDGCHC 2 cut(s) 207, 834
MlsI TGGCCA 1 cut(s) 171
MluCI AATT 3 cut(s) 150, 176, 528
MluNI TGGCCA 1 cut(s) 171
MmeI TCCRAC 3 cut(s) 467, 596, 614
MnlI CCTC 8 cut(s) 140, 207, 222, 631, 763, 766, 769, 953
Mox20I TGGCCA 1 cut(s) 171
MroXI GAANNNNTTC 3 cut(s) 356, 417, 872
MscI TGGCCA 1 cut(s) 171
MseI TTAA 4 cut(s) 89, 461, 627, 633
Msp20I TGGCCA 1 cut(s) 171
MspCI CTTAAG 1 cut(s) 88
MspI CCGG 1 cut(s) 738
MspR9I CCNGG 1 cut(s) 164
MvaI CCWGG 1 cut(s) 164
NdeI CATATG 1 cut(s) 977
NdeII GATC 2 cut(s) 705, 745
NlaIII CATG 7 cut(s) 104, 176, 313, 347, 365, 410, 1016
NmuCI GTSAC 1 cut(s) 95
NspI RCATGY 1 cut(s) 1016
PaeI GCATGC 1 cut(s) 1016
PaeR7I CTCGAG 2 cut(s) 144, 302
PagI TCATGA 1 cut(s) 343
PceI AGGCCT 1 cut(s) 752
PdmI GAANNNNTTC 3 cut(s) 356, 417, 872
PfeI GAWTC 3 cut(s) 224, 328, 352
PfoI TCCNGGA 1 cut(s) 162
PinAI ACCGGT 1 cut(s) 737
PkrI GCNGC 2 cut(s) 808, 967
PshBI ATTAAT 1 cut(s) 627
Psp124BI GAGCTC 2 cut(s) 207, 834
Psp6I CCWGG 1 cut(s) 162
PspGI CCWGG 1 cut(s) 162
RsaI GTAC 5 cut(s) 128, 157, 513, 864, 931
RsaNI GTAC 5 cut(s) 127, 156, 512, 863, 930
SacI GAGCTC 2 cut(s) 207, 834
SaqAI TTAA 4 cut(s) 89, 461, 627, 633
SatI GCNGC 2 cut(s) 807, 966
Sau3AI GATC 2 cut(s) 705, 745
ScaI AGTACT 1 cut(s) 864
ScrFI CCNGG 1 cut(s) 164
SduI GDGCHC 2 cut(s) 207, 834
SetI ASST 8 cut(s) 64, 207, 233, 291, 441, 448, 717, 834
SfaNI GCATC 2 cut(s) 445, 595
SfcI CTRYAG 1 cut(s) 666
Sfr274I CTCGAG 2 cut(s) 144, 302
SlaI CTCGAG 2 cut(s) 144, 302
SmlI CTYRAG 5 cut(s) 88, 144, 200, 302, 760
SmoI CTYRAG 5 cut(s) 88, 144, 200, 302, 760
SphI GCATGC 1 cut(s) 1016
Sse9I AATT 3 cut(s) 150, 176, 528
SseBI AGGCCT 1 cut(s) 752
SsiI CCGC 1 cut(s) 74
SspMI CTAG 3 cut(s) 521, 621, 722
SstI GAGCTC 2 cut(s) 207, 834
StuI AGGCCT 1 cut(s) 752
StyD4I CCNGG 1 cut(s) 162
StyI CCWWGG 1 cut(s) 715
TaaI ACNGT 2 cut(s) 647, 774
TaqI TCGA 4 cut(s) 145, 219, 227, 303
TasI AATT 3 cut(s) 150, 176, 528
TatI WGTACW 3 cut(s) 155, 511, 862
TfiI GAWTC 3 cut(s) 224, 328, 352
Tru1I TTAA 4 cut(s) 89, 461, 627, 633
Tru9I TTAA 4 cut(s) 89, 461, 627, 633
TscAI CASTG 1 cut(s) 977
TseFI GTSAC 1 cut(s) 95
TseI GCWGC 2 cut(s) 806, 965
Tsp45I GTSAC 1 cut(s) 95
TspDTI ATGAA 6 cut(s) 117, 189, 326, 332, 423, 522
TspGWI ACGGA 2 cut(s) 58, 963
TspRI CASTG 1 cut(s) 977
Vha464I CTTAAG 1 cut(s) 88
VspI ATTAAT 1 cut(s) 627
XapI RAATTY 2 cut(s) 150, 176
XbaI TCTAGA 1 cut(s) 520
XceI RCATGY 1 cut(s) 1016
XcmI CCANNNNNNNNNTGG 1 cut(s) 604
XhoI CTCGAG 2 cut(s) 144, 302
XmnI GAANNNNTTC 3 cut(s) 356, 417, 872
XspI CTAG 3 cut(s) 521, 621, 722
ZrmI AGTACT 1 cut(s) 864
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.