Prupe.5G012500_v2.0.a1

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp05
OG0000508
Physical Location & Seq
Reverse (-)
1341141 .. 1343613
2473 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.5G012500.3

Sequence Viewer

Length: 804 bp
ATGAGGGCTACTTCAGAATCAGCAGAGTGGATATTTTTAGCCACTGGCCTTGGCCTAGAGATCTTGTCAGCTGCTTGTGATCAGGCTTCCTCCCCAAGAACACCCCACTATGCCCTATTTGGGATGCTGTTCGCTATTGCAGCTGTGCTCATTTCCATCTGGGAGCTAATTTACAGAGGTAAAAAGGAAAGAGTTGTGTTGAGGAGATGGGGAATGCTCTGGTGGTTTTATCATACACCACCTCCTCGACATACGCCTTTTGGTACTCTTCCTGACATTTATGGACTAGTTGCTGGCATCTCACAGTGCATTTGCTCTATAGTTCAGTATGTTTACTGCCTTCGGCATGCTAATAGTCCTTTCAAAGCATCCCTTTTGCCTGCCATATTTCTTATGTGTTTAGGTGGTTCAAAACTATGTAATAACCGAATGAACGCGAATACCACTGATAACAAGGACTCGTGCGAAAATTCTTCAAGCACGGAAGAAACTTCGTGGCATGCAATCAAGGCAGATCTACCATTTCTTTATGATCAAGAGGTTGAAAGCAACATAGAGCAGCATCGGCTGCAAGAGCAGCGGCGGCAACCGCATGAGCAGCAGCAGCTGCTGGAGATGTGGCTGCATGAACAACATGAGCAGTATGAGCAGTATGAACAGCAGAAGCAGCGGCGGCGGCGAGAGCTTCAGCGGCTACAGGAGCAGCAAGAGCGACTGCAGGAGCTGCAGCGACAGCTGGAGCTGGAGCAGGAGCAAGAGCAGCAGAAGTTCCTGCGACATCGGCGAGTAGTGAAGGCGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

268

Amino Acids

31.53

Weight (kDa)

6.56

Isoelectric Point (pI)

81.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 437
AciI CCGC 8 cut(s) 580, 583, 590, 670, 673, 676, 691, 797
AcsI RAATTY 1 cut(s) 469
AcuI CTGAAG 1 cut(s) 671
AfaI GTAC 1 cut(s) 265
AfiI CCNNNNNNNGG 1 cut(s) 120
AgsI TTSAA 4 cut(s) 364, 411, 477, 545
AhlI ACTAGT 1 cut(s) 286
AluBI AGCT 8 cut(s) 71, 143, 166, 607, 685, 724, 736, 742
AluI AGCT 8 cut(s) 71, 143, 166, 607, 685, 724, 736, 742
Alw21I GWGCWC 1 cut(s) 150
AlwNI CAGNNNCTG 3 cut(s) 607, 610, 724
AoxI GGCC 2 cut(s) 46, 52
ApoI RAATTY 1 cut(s) 469
BauI CACGAG 1 cut(s) 460
Bbv12I GWGCWC 1 cut(s) 150
BccI CCATC 2 cut(s) 164, 201
BclI TGATCA 2 cut(s) 79, 532
BcuI ACTAGT 1 cut(s) 286
BfaI CTAG 2 cut(s) 56, 287
BfmI CTRYAG 4 cut(s) 318, 695, 716, 725
BglII AGATCT 2 cut(s) 60, 514
BmsI GCATC 4 cut(s) 114, 306, 377, 571
BpmI CTGGAG 3 cut(s) 632, 758, 764
BsaJI CCNNGG 1 cut(s) 49
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bsc4I CCNNNNNNNGG 1 cut(s) 120
Bse1I ACTGG 1 cut(s) 49
BseDI CCNNGG 1 cut(s) 49
BseGI GGATG 2 cut(s) 129, 368
BseLI CCNNNNNNNGG 1 cut(s) 120
BseNI ACTGG 1 cut(s) 49
BseRI GAGGAG 2 cut(s) 217, 234
Bsh1236I CGCG 1 cut(s) 437
BshFI GGCC 2 cut(s) 48, 54
BsiHKAI GWGCWC 1 cut(s) 150
BslI CCNNNNNNNGG 1 cut(s) 120
BsmI GAATGC 1 cut(s) 219
BsnI GGCC 2 cut(s) 48, 54
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 4 cut(s) 60, 79, 514, 532
BspACI CCGC 8 cut(s) 580, 583, 590, 670, 673, 676, 691, 797
BspANI GGCC 2 cut(s) 48, 54
BspFNI CGCG 1 cut(s) 437
BspMAI CTGCAG 2 cut(s) 720, 729
BsrI ACTGG 1 cut(s) 49
BssECI CCNNGG 1 cut(s) 49
BssMI GATC 4 cut(s) 60, 79, 514, 532
BssSI CACGAG 1 cut(s) 460
BssT1I CCWWGG 1 cut(s) 49
Bst2BI CACGAG 1 cut(s) 460
Bst4CI ACNGT 1 cut(s) 306
Bst6I CTCTTC 1 cut(s) 273
BstAPI GCANNNNNTGC 3 cut(s) 568, 607, 724
BstC8I GCNNGC 4 cut(s) 295, 348, 381, 501
BstF5I GGATG 2 cut(s) 129, 368
BstFNI CGCG 1 cut(s) 437
BstKTI GATC 4 cut(s) 63, 82, 517, 535
BstMBI GATC 4 cut(s) 60, 79, 514, 532
BstNSI RCATGY 2 cut(s) 350, 503
BstSFI CTRYAG 4 cut(s) 318, 695, 716, 725
BstUI CGCG 1 cut(s) 437
BstX2I RGATCY 2 cut(s) 60, 514
BstYI RGATCY 2 cut(s) 60, 514
BsuRI GGCC 2 cut(s) 48, 54
BtsCI GGATG 2 cut(s) 129, 368
BtsIMutI CAGTG 3 cut(s) 42, 311, 444
Cac8I GCNNGC 4 cut(s) 295, 348, 381, 501
CaiI CAGNNNCTG 3 cut(s) 607, 610, 724
Csp6I GTAC 1 cut(s) 264
CspCI CAANNNNNGTGG 2 cut(s) 31, 66
CviAII CATG 5 cut(s) 347, 500, 593, 626, 635
CviQI GTAC 1 cut(s) 264
DpnI GATC 4 cut(s) 62, 81, 516, 534
DpnII GATC 4 cut(s) 60, 79, 514, 532
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
Eco130I CCWWGG 1 cut(s) 49
Eco57I CTGAAG 1 cut(s) 671
EcoT14I CCWWGG 1 cut(s) 49
ErhI CCWWGG 1 cut(s) 49
FaeI CATG 5 cut(s) 350, 503, 596, 629, 638
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FatI CATG 5 cut(s) 346, 499, 592, 625, 634
FbaI TGATCA 2 cut(s) 79, 532
FokI GGATG 2 cut(s) 136, 355
FspBI CTAG 2 cut(s) 56, 287
GsuI CTGGAG 3 cut(s) 632, 758, 764
HaeIII GGCC 2 cut(s) 48, 54
Hin1II CATG 5 cut(s) 350, 503, 596, 629, 638
HinfI GANTC 2 cut(s) 17, 458
Hpy166II GTNNAC 1 cut(s) 334
Hpy188I TCNGA 1 cut(s) 16
Hpy188III TCNNGA 2 cut(s) 272, 536
Hpy8I GTNNAC 1 cut(s) 334
HpyAV CCTTC 2 cut(s) 350, 787
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4V TGCA 7 cut(s) 140, 309, 503, 571, 625, 718, 727
Hsp92II CATG 5 cut(s) 350, 503, 596, 629, 638
Ksp22I TGATCA 2 cut(s) 79, 532
Kzo9I GATC 4 cut(s) 60, 79, 514, 532
LmnI GCTCC 6 cut(s) 163, 700, 721, 739, 745, 751
LweI GCATC 4 cut(s) 114, 306, 377, 571
MaeI CTAG 2 cut(s) 56, 287
MalI GATC 4 cut(s) 62, 81, 516, 534
MboI GATC 4 cut(s) 60, 79, 514, 532
MboII GAAGA 3 cut(s) 260, 465, 497
MflI RGATCY 2 cut(s) 60, 514
MhlI GDGCHC 1 cut(s) 150
MluCI AATT 2 cut(s) 168, 469
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 6 cut(s) 100, 170, 195, 252, 255, 532
MspA1I CMGCKG 7 cut(s) 71, 143, 580, 607, 670, 691, 736
Mva1269I GAATGC 1 cut(s) 219
MvnI CGCG 1 cut(s) 437
NdeII GATC 4 cut(s) 60, 79, 514, 532
NlaIII CATG 5 cut(s) 350, 503, 596, 629, 638
NspI RCATGY 2 cut(s) 350, 503
PaeI GCATGC 2 cut(s) 350, 503
PctI GAATGC 1 cut(s) 219
PfeI GAWTC 1 cut(s) 17
PleI GAGTC 1 cut(s) 452
PpsI GAGTC 1 cut(s) 452
PstI CTGCAG 2 cut(s) 720, 729
PstNI CAGNNNCTG 3 cut(s) 607, 610, 724
PsuI RGATCY 2 cut(s) 60, 514
PvuII CAGCTG 4 cut(s) 71, 143, 607, 736
RsaI GTAC 1 cut(s) 265
RsaNI GTAC 1 cut(s) 264
Sau3AI GATC 4 cut(s) 60, 79, 514, 532
SchI GAGTC 1 cut(s) 452
SduI GDGCHC 1 cut(s) 150
SfaNI GCATC 4 cut(s) 114, 306, 377, 571
SfcI CTRYAG 4 cut(s) 318, 695, 716, 725
SpeI ACTAGT 1 cut(s) 286
SphI GCATGC 2 cut(s) 350, 503
Sse9I AATT 2 cut(s) 168, 469
SsiI CCGC 8 cut(s) 580, 583, 590, 670, 673, 676, 691, 797
SspMI CTAG 2 cut(s) 56, 287
StyI CCWWGG 1 cut(s) 49
TaaI ACNGT 1 cut(s) 306
TaqI TCGA 1 cut(s) 247
TasI AATT 2 cut(s) 168, 469
TauI GCSGC 6 cut(s) 583, 586, 673, 676, 679, 694
TfiI GAWTC 1 cut(s) 17
TscAI CASTG 3 cut(s) 49, 311, 451
TspDTI ATGAA 3 cut(s) 446, 642, 669
TspGWI ACGGA 1 cut(s) 497
TspRI CASTG 3 cut(s) 49, 311, 451
XapI RAATTY 1 cut(s) 469
XceI RCATGY 2 cut(s) 350, 503
XspI CTAG 2 cut(s) 56, 287
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.