Rw6G018410

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000508
Physical Location & Seq
Reverse (-)
36602621 .. 36604133
1513 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G018410.1

Sequence Viewer

Length: 960 bp
ATGGTGTTGAACAAGCACAGTGTGTCTATTCCATTACGGATATCTATTCCTTTTAATTCTCTATGGGCGTGTGATGTCTTCCTAAGTTGCGAGCATGAAGACATCTTCTCCAACCACTTGTACTACAAATTGCAGTCTCGAGGAATTAGTATTTTCCAGGATGGCCAAGGATGCTTGCAAAGAAACACAACTCCTCTTGAGCTCTTTGGTGCAATCGAAGAATCGATGTTTGCAGTTATTGTTCTCTCGCAAAACTATGCTTCTTCGCCCCGGCGCTTGAATGAACTTTTAAAAATTCTAGAGTGCATGAAAGATCGGAACAGGATTCTGCCGGTATTTCATGATGTTAATCTGAATGAAGTACAGGTTATAGAAGAAATTACTGAAGCACTGTGGAACAAACTACATTCAAGAATCAGTCCAACGGAAAAACTGCAGCCGACATCCACACACAACTTGGTCGCATTCACTAGCATTAGTGTTAATGAGGACTACCGTGACCATAACCATCCAATACACATTACAGATATGACGCCTTCTTCAGTAGAGTGGAACTTTTTGATCACTAACCTTGGCCTAGAGATTTTGTCAGCTGCTTTTGATCAGGCTTCCTCGTCAAGTAAGCCACACTATGCACTGTTTGGTATGCTTTTTTCTATTGCAGCCTTGTTCACTTGCATTTGGGAGCTCATTTACAAGGGCATAAAGAATAGAGTAGTATTGAAGAAGTTCGGAAAGCTCTGGTGGTTTTATTATCCACATCCCCACAATAGCATGCCTTTCGGTACTCTCCCTGAGATTTACGGATTAATTGGAAGCATCGCTCAGTGCATATGCTCTACAACTCAATACATTTACTTCTCTTGGCATGCTGATAGTCCCATAAAACTCTCACTTTTACCAGCAATCTTTCTTTTCTGTTTGGCTGGTTCAAGACTAAATAGGAATCAAAACCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

319

Amino Acids

36.57

Weight (kDa)

6.69

Isoelectric Point (pI)

58.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 26 - 121 4.2e-16 TIR domain
TIR_2 PF13676 26 - 118 4.8e-10 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000508)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06570 FvH4_2g06640 FvH4_2g14130 FvH4_2g14140 FvH4_2g14140 FvH4_2g36090 FvH4_2g36090 FvH4_2g36100
malus_domestica MD11G1218200.v1.1 MD15G1057100.v1.1 MD15G1057200.v1.1
prunus_persica Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.1G197200_v2.0.a1 Prupe.4G127700_v2.0.a1 Prupe.5G011700_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G011800_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012300_v2.0.a1 Prupe.5G012400_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012500_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.5G012700_v2.0.a1 Prupe.8G119700_v2.0.a1 Prupe.8G119700_v2.0.a1
pyrus_communis pycom02g13080 pycom05g07020 pycom15g05370 pycom15g05380
rosa_chinensis RchiOBHm_Chr1g0372601 RchiOBHm_Chr6g0258611 RchiOBHm_Chr6g0276221 RchiOBHm_Chr6g0276231 RchiOBHm_Chr6g0276251 RchiOBHm_Chr6g0276271 RchiOBHm_Chr6g0276281 RchiOBHm_Chr6g0276291
rosa_laevigata RLG00000013406 RLG00000013407 RLG00000013408 RLG00000013617
rosa_multiflora Rmu_sc0001663.1_g000017 Rmu_sc0003665.1_g000003 Rmu_sc0004048.1_g000003 Rmu_sc0004048.1_g000006 Rmu_sc0004048.1_g000008 Rmu_sc0004049.1_g000006 Rmu_sc0004049.1_g000008 Rmu_sc0004049.1_g000010 Rmu_ssc0000213.1_g000005
rosa_roxburghii Rroxscaffold_178G00437490 Rroxscaffold_7G00192430 Rroxscaffold_7G00192440 Rroxscaffold_7G00192480 Rroxscaffold_7G00205750
rosa_rugosa Rorug05G0581900 Rorug05G0582500 Rorug06G0074900 Rorug06G0099400 Rorug06G0099600
rosa_samantha Rh6AG098600 Rh6AG190000 Rh6AG210800 Rh6AG210900 Rh6BG090400 Rh6BG193400 Rh6BG215000 Rh6BG215100 Rh6CG087300 Rh6DG081500 Rh6DG184600 Rh6DG207700
rosa_wichuraiana Rw6G008540 Rw6G018370 Rw6G018380 Rw6G018410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcoI YGGCCR 1 cut(s) 163
AcsI RAATTY 1 cut(s) 294
AcuI CTGAAG 2 cut(s) 405, 525
AcyI GRCGYC 1 cut(s) 533
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 3 cut(s) 122, 363, 787
AgsI TTSAA 5 cut(s) 10, 280, 411, 724, 933
AjnI CCWGG 1 cut(s) 156
AluBI AGCT 4 cut(s) 202, 593, 688, 739
AluI AGCT 4 cut(s) 202, 593, 688, 739
Alw21I GWGCWC 2 cut(s) 204, 690
Alw26I GTCTC 1 cut(s) 141
Ama87I CYCGRG 1 cut(s) 138
AoxI GGCC 2 cut(s) 163, 574
ApeKI GCWGC 3 cut(s) 436, 593, 662
ApoI RAATTY 1 cut(s) 294
AseI ATTAAT 1 cut(s) 809
Asp700I GAANNNNTTC 1 cut(s) 728
AspLEI GCGC 1 cut(s) 276
AsuC2I CCSGG 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 138
BalI TGGCCA 1 cut(s) 165
BanII GRGCYC 2 cut(s) 204, 690
BbsI GAAGAC 2 cut(s) 70, 105
Bbv12I GWGCWC 2 cut(s) 204, 690
BbvI GCAGC 3 cut(s) 448, 580, 674
BccI CCATC 2 cut(s) 155, 516
BcgI CGANNNNNNTGC 2 cut(s) 763, 797
BciT130I CCWGG 1 cut(s) 158
BclI TGATCA 2 cut(s) 561, 601
BcnI CCSGG 1 cut(s) 271
BcoDI GTCTC 1 cut(s) 141
BfaI CTAG 3 cut(s) 299, 471, 578
BfmI CTRYAG 1 cut(s) 434
BfoI RGCGCY 1 cut(s) 277
BisI GCNGC 3 cut(s) 437, 594, 663
BlsI GCNGC 3 cut(s) 438, 595, 664
Bme1390I CCNGG 2 cut(s) 158, 271
BmeT110I CYCGRG 1 cut(s) 138
BmrFI CCNGG 2 cut(s) 158, 271
BmsI GCATC 2 cut(s) 161, 828
BpiI GAAGAC 2 cut(s) 70, 105
BpuEI CTTGAG 1 cut(s) 218
BpuMI CCSGG 1 cut(s) 271
Bsa29I ATCGAT 1 cut(s) 224
BsaBI GATNNNNATC 1 cut(s) 348
BsaHI GRCGYC 1 cut(s) 533
BsaJI CCNNGG 3 cut(s) 166, 269, 571
BsaXI ACNNNNNCTCC 2 cut(s) 92, 122
Bse118I RCCGGY 1 cut(s) 331
Bse8I GATNNNNATC 1 cut(s) 348
BseBI CCWGG 1 cut(s) 158
BseCI ATCGAT 1 cut(s) 224
BseDI CCNNGG 3 cut(s) 166, 269, 571
BseGI GGATG 5 cut(s) 166, 176, 443, 508, 760
BseJI GATNNNNATC 1 cut(s) 348
BseMII CTCAG 2 cut(s) 786, 839
BseRI GAGGAG 1 cut(s) 183
BseXI GCAGC 3 cut(s) 448, 580, 674
BshFI GGCC 2 cut(s) 165, 576
BshVI ATCGAT 1 cut(s) 224
BsiHKAI GWGCWC 2 cut(s) 204, 690
BsiHKCI CYCGRG 1 cut(s) 138
BsiSI CCGG 2 cut(s) 271, 332
BslFI GGGAC 1 cut(s) 864
BsmAI GTCTC 1 cut(s) 141
BsmFI GGGAC 1 cut(s) 864
BsmI GAATGC 1 cut(s) 464
BsnI GGCC 2 cut(s) 165, 576
BsoBI CYCGRG 1 cut(s) 138
Bsp1286I GDGCHC 2 cut(s) 204, 690
Bsp143I GATC 3 cut(s) 313, 561, 601
BspANI GGCC 2 cut(s) 165, 576
BspCNI CTCAG 2 cut(s) 787, 838
BspDI ATCGAT 1 cut(s) 224
BspHI TCATGA 1 cut(s) 340
BspMAI CTGCAG 1 cut(s) 438
BsrFI RCCGGY 1 cut(s) 331
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 3 cut(s) 166, 269, 571
BssMI GATC 3 cut(s) 313, 561, 601
BssNI GRCGYC 1 cut(s) 533
BssT1I CCWWGG 2 cut(s) 166, 571
Bst2UI CCWGG 1 cut(s) 158
Bst4CI ACNGT 4 cut(s) 20, 393, 497, 639
BstACI GRCGYC 1 cut(s) 533
BstC8I GCNNGC 4 cut(s) 92, 176, 776, 870
BstDEI CTNAG 3 cut(s) 83, 795, 825
BstF5I GGATG 5 cut(s) 166, 176, 443, 508, 760
BstH2I RGCGCY 1 cut(s) 277
BstHHI GCGC 1 cut(s) 276
BstKTI GATC 3 cut(s) 316, 564, 604
BstMAI GTCTC 1 cut(s) 141
BstMBI GATC 3 cut(s) 313, 561, 601
BstMWI GCNNNNNNNGC 1 cut(s) 171
BstNI CCWGG 1 cut(s) 158
BstNSI RCATGY 2 cut(s) 778, 872
BstSCI CCNGG 2 cut(s) 156, 269
BstSFI CTRYAG 1 cut(s) 434
BstV1I GCAGC 3 cut(s) 448, 580, 674
BstV2I GAAGAC 2 cut(s) 70, 105
Bsu15I ATCGAT 1 cut(s) 224
BsuRI GGCC 2 cut(s) 165, 576
BsuTUI ATCGAT 1 cut(s) 224
BtgZI GCGATG 1 cut(s) 805
BtsCI GGATG 5 cut(s) 166, 176, 443, 508, 760
BtsIMutI CAGTG 4 cut(s) 25, 389, 635, 833
Cac8I GCNNGC 4 cut(s) 92, 176, 776, 870
CciI TCATGA 1 cut(s) 340
CfoI GCGC 1 cut(s) 276
Cfr10I RCCGGY 1 cut(s) 331
ClaI ATCGAT 1 cut(s) 224
CseI GACGC 1 cut(s) 541
Csp6I GTAC 3 cut(s) 121, 362, 786
CviAII CATG 5 cut(s) 95, 307, 341, 775, 869
CviQI GTAC 3 cut(s) 121, 362, 786
DdeI CTNAG 3 cut(s) 83, 795, 825
DpnI GATC 3 cut(s) 315, 563, 603
DpnII GATC 3 cut(s) 313, 561, 601
DraI TTTAAA 1 cut(s) 291
DraIII CACNNNGTG 1 cut(s) 22
EaeI YGGCCR 1 cut(s) 163
Ecl136II GAGCTC 2 cut(s) 202, 688
Eco130I CCWWGG 2 cut(s) 166, 571
Eco24I GRGCYC 2 cut(s) 204, 690
Eco32I GATATC 1 cut(s) 42
Eco53kI GAGCTC 2 cut(s) 202, 688
Eco57I CTGAAG 2 cut(s) 405, 525
Eco88I CYCGRG 1 cut(s) 138
EcoICRI GAGCTC 2 cut(s) 202, 688
EcoRII CCWGG 1 cut(s) 156
EcoRV GATATC 1 cut(s) 42
EcoT14I CCWWGG 2 cut(s) 166, 571
EcoT38I GRGCYC 2 cut(s) 204, 690
ErhI CCWWGG 2 cut(s) 166, 571
FaeI CATG 5 cut(s) 98, 310, 344, 778, 872
FaqI GGGAC 1 cut(s) 864
FatI CATG 5 cut(s) 94, 306, 340, 774, 868
FauNDI CATATG 1 cut(s) 833
FbaI TGATCA 2 cut(s) 561, 601
Fnu4HI GCNGC 3 cut(s) 437, 594, 663
FokI GGATG 5 cut(s) 173, 183, 430, 495, 747
FriOI GRGCYC 2 cut(s) 204, 690
Fsp4HI GCNGC 3 cut(s) 437, 594, 663
FspBI CTAG 3 cut(s) 299, 471, 578
GlaI GCGC 1 cut(s) 275
GluI GCNGC 3 cut(s) 437, 594, 663
HaeII RGCGCY 1 cut(s) 277
HaeIII GGCC 2 cut(s) 165, 576
HapII CCGG 2 cut(s) 271, 332
HgaI GACGC 1 cut(s) 541
HhaI GCGC 1 cut(s) 276
Hin1I GRCGYC 1 cut(s) 533
Hin1II CATG 5 cut(s) 98, 310, 344, 778, 872
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HinfI GANTC 4 cut(s) 221, 325, 414, 946
HpaII CCGG 2 cut(s) 271, 332
Hpy166II GTNNAC 1 cut(s) 672
Hpy188I TCNGA 3 cut(s) 318, 354, 734
Hpy188III TCNNGA 6 cut(s) 138, 197, 299, 341, 411, 933
Hpy8I GTNNAC 1 cut(s) 672
HpyAV CCTTC 1 cut(s) 546
HpyCH4III ACNGT 4 cut(s) 20, 393, 497, 639
HpyF10VI GCNNNNNNNGC 1 cut(s) 171
HpyF3I CTNAG 3 cut(s) 83, 795, 825
Hsp92I GRCGYC 1 cut(s) 533
Hsp92II CATG 5 cut(s) 98, 310, 344, 778, 872
HspAI GCGC 1 cut(s) 274
Ksp22I TGATCA 2 cut(s) 561, 601
Kzo9I GATC 3 cut(s) 313, 561, 601
LmnI GCTCC 1 cut(s) 685
Lsp1109I GCAGC 3 cut(s) 448, 580, 674
LweI GCATC 2 cut(s) 161, 828
MaeI CTAG 3 cut(s) 299, 471, 578
MaeIII GTNAC 1 cut(s) 497
MalI GATC 3 cut(s) 315, 563, 603
MboI GATC 3 cut(s) 313, 561, 601
MboII GAAGA 8 cut(s) 70, 97, 110, 230, 255, 386, 531, 736
MhlI GDGCHC 2 cut(s) 204, 690
MlsI TGGCCA 1 cut(s) 165
MluCI AATT 6 cut(s) 55, 128, 144, 294, 378, 810
MluNI TGGCCA 1 cut(s) 165
MmeI TCCRAC 2 cut(s) 135, 446
MnlI CCTC 4 cut(s) 134, 204, 481, 622
Mox20I TGGCCA 1 cut(s) 165
MroXI GAANNNNTTC 1 cut(s) 728
MscI TGGCCA 1 cut(s) 165
MseI TTAA 5 cut(s) 54, 290, 348, 483, 809
Msp20I TGGCCA 1 cut(s) 165
MspA1I CMGCKG 1 cut(s) 593
MspI CCGG 2 cut(s) 271, 332
MspR9I CCNGG 2 cut(s) 158, 271
Mva1269I GAATGC 1 cut(s) 464
MvaI CCWGG 1 cut(s) 158
MwoI GCNNNNNNNGC 1 cut(s) 171
NciI CCSGG 1 cut(s) 271
NdeI CATATG 1 cut(s) 833
NdeII GATC 3 cut(s) 313, 561, 601
NlaIII CATG 5 cut(s) 98, 310, 344, 778, 872
NmuCI GTSAC 1 cut(s) 497
NspI RCATGY 2 cut(s) 778, 872
PaeI GCATGC 2 cut(s) 778, 872
PaeR7I CTCGAG 1 cut(s) 138
PagI TCATGA 1 cut(s) 340
PctI GAATGC 1 cut(s) 464
PdmI GAANNNNTTC 1 cut(s) 728
PfeI GAWTC 4 cut(s) 221, 325, 414, 946
PfoI TCCNGGA 1 cut(s) 156
PkrI GCNGC 3 cut(s) 438, 595, 664
PshBI ATTAAT 1 cut(s) 809
Psp124BI GAGCTC 2 cut(s) 204, 690
Psp6I CCWGG 1 cut(s) 156
PspGI CCWGG 1 cut(s) 156
PstI CTGCAG 1 cut(s) 438
PvuII CAGCTG 1 cut(s) 593
RsaI GTAC 3 cut(s) 122, 363, 787
RsaNI GTAC 3 cut(s) 121, 362, 786
SacI GAGCTC 2 cut(s) 204, 690
SaqAI TTAA 5 cut(s) 54, 290, 348, 483, 809
SatI GCNGC 3 cut(s) 437, 594, 663
Sau3AI GATC 3 cut(s) 313, 561, 601
ScrFI CCNGG 2 cut(s) 158, 271
SduI GDGCHC 2 cut(s) 204, 690
SetI ASST 6 cut(s) 204, 369, 573, 595, 690, 741
SfaNI GCATC 2 cut(s) 161, 828
SfcI CTRYAG 1 cut(s) 434
Sfr274I CTCGAG 1 cut(s) 138
SlaI CTCGAG 1 cut(s) 138
SmlI CTYRAG 2 cut(s) 138, 197
SmoI CTYRAG 2 cut(s) 138, 197
SphI GCATGC 2 cut(s) 778, 872
Sse9I AATT 6 cut(s) 55, 128, 144, 294, 378, 810
SspMI CTAG 3 cut(s) 299, 471, 578
SstI GAGCTC 2 cut(s) 204, 690
StyD4I CCNGG 2 cut(s) 156, 269
StyI CCWWGG 2 cut(s) 166, 571
TaaI ACNGT 4 cut(s) 20, 393, 497, 639
TaqI TCGA 3 cut(s) 139, 216, 224
TasI AATT 6 cut(s) 55, 128, 144, 294, 378, 810
TatI WGTACW 2 cut(s) 120, 361
TfiI GAWTC 4 cut(s) 221, 325, 414, 946
Tru1I TTAA 5 cut(s) 54, 290, 348, 483, 809
Tru9I TTAA 5 cut(s) 54, 290, 348, 483, 809
TscAI CASTG 4 cut(s) 25, 396, 642, 833
TseFI GTSAC 1 cut(s) 497
TseI GCWGC 3 cut(s) 436, 593, 662
Tsp45I GTSAC 1 cut(s) 497
TspDTI ATGAA 5 cut(s) 111, 297, 323, 329, 372
TspGWI ACGGA 3 cut(s) 52, 440, 819
TspRI CASTG 4 cut(s) 25, 396, 642, 833
VspI ATTAAT 1 cut(s) 809
XapI RAATTY 1 cut(s) 294
XbaI TCTAGA 1 cut(s) 298
XceI RCATGY 2 cut(s) 778, 872
XcmI CCANNNNNNNNNTGG 1 cut(s) 454
XhoI CTCGAG 1 cut(s) 138
XmnI GAANNNNTTC 1 cut(s) 728
XspI CTAG 3 cut(s) 299, 471, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.