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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 18 |
| AciI | CCGC | 1 cut(s) | 62 |
| AclWI | GGATC | 1 cut(s) | 625 |
| AcsI | RAATTY | 4 cut(s) | 286, 516, 568, 602 |
| AcuI | CTGAAG | 2 cut(s) | 35, 444 |
| AcyI | GRCGYC | 1 cut(s) | 15 |
| AfaI | GTAC | 1 cut(s) | 66 |
| AgsI | TTSAA | 8 cut(s) | 35, 169, 285, 457, 589, 595, 665, 697 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 555, 587 |
| AluBI | AGCT | 1 cut(s) | 422 |
| AluI | AGCT | 1 cut(s) | 422 |
| Alw26I | GTCTC | 1 cut(s) | 117 |
| AlwI | GGATC | 1 cut(s) | 625 |
| ApoI | RAATTY | 4 cut(s) | 286, 516, 568, 602 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 461, 493 |
| AspS9I | GGNCC | 1 cut(s) | 209 |
| AsuHPI | GGTGA | 2 cut(s) | 191, 572 |
| AvaII | GGWCC | 1 cut(s) | 209 |
| BccI | CCATC | 3 cut(s) | 170, 332, 551 |
| BcoDI | GTCTC | 1 cut(s) | 117 |
| Bme18I | GGWCC | 1 cut(s) | 209 |
| BmgT120I | GGNCC | 1 cut(s) | 209 |
| BmiI | GGNNCC | 1 cut(s) | 187 |
| BmsI | GCATC | 3 cut(s) | 36, 87, 165 |
| Bsa29I | ATCGAT | 1 cut(s) | 527 |
| BsaBI | GATNNNNATC | 1 cut(s) | 616 |
| BsaHI | GRCGYC | 1 cut(s) | 15 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 246, 276, 614, 644 |
| Bse8I | GATNNNNATC | 1 cut(s) | 616 |
| BseCI | ATCGAT | 1 cut(s) | 527 |
| BseGI | GGATG | 2 cut(s) | 51, 562 |
| BseJI | GATNNNNATC | 1 cut(s) | 616 |
| BshVI | ATCGAT | 1 cut(s) | 527 |
| BsmAI | GTCTC | 1 cut(s) | 117 |
| BsmBI | CGTCTC | 1 cut(s) | 117 |
| Bsp143I | GATC | 3 cut(s) | 151, 444, 617 |
| BspACI | CCGC | 1 cut(s) | 62 |
| BspDI | ATCGAT | 1 cut(s) | 527 |
| BspHI | TCATGA | 1 cut(s) | 10 |
| BspLI | GGNNCC | 1 cut(s) | 187 |
| BspPI | GGATC | 1 cut(s) | 625 |
| BssMI | GATC | 3 cut(s) | 151, 444, 617 |
| BssNI | GRCGYC | 1 cut(s) | 15 |
| Bst4CI | ACNGT | 1 cut(s) | 185 |
| Bst6I | CTCTTC | 2 cut(s) | 590, 652 |
| BstACI | GRCGYC | 1 cut(s) | 15 |
| BstF5I | GGATG | 2 cut(s) | 51, 562 |
| BstKTI | GATC | 3 cut(s) | 154, 447, 620 |
| BstMAI | GTCTC | 1 cut(s) | 117 |
| BstMBI | GATC | 3 cut(s) | 151, 444, 617 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 449 |
| Bsu15I | ATCGAT | 1 cut(s) | 527 |
| BsuTUI | ATCGAT | 1 cut(s) | 527 |
| BtsCI | GGATG | 2 cut(s) | 51, 562 |
| CciI | TCATGA | 1 cut(s) | 10 |
| Cfr13I | GGNCC | 1 cut(s) | 209 |
| ClaI | ATCGAT | 1 cut(s) | 527 |
| Csp6I | GTAC | 1 cut(s) | 65 |
| CviAII | CATG | 2 cut(s) | 11, 28 |
| CviJI | RGCY | 3 cut(s) | 361, 392, 422 |
| CviKI_1 | RGCY | 3 cut(s) | 361, 392, 422 |
| CviQI | GTAC | 1 cut(s) | 65 |
| DpnI | GATC | 3 cut(s) | 153, 446, 619 |
| DpnII | GATC | 3 cut(s) | 151, 444, 617 |
| Eam1104I | CTCTTC | 2 cut(s) | 590, 652 |
| EarI | CTCTTC | 2 cut(s) | 590, 652 |
| Eco47I | GGWCC | 1 cut(s) | 209 |
| Eco57I | CTGAAG | 2 cut(s) | 35, 444 |
| Esp3I | CGTCTC | 1 cut(s) | 117 |
| FaeI | CATG | 2 cut(s) | 14, 31 |
| FaiI | YATR | 7 cut(s) | 12, 29, 69, 202, 276, 372, 512 |
| FatI | CATG | 2 cut(s) | 10, 27 |
| FokI | GGATG | 2 cut(s) | 58, 569 |
| Hin1I | GRCGYC | 1 cut(s) | 15 |
| Hin1II | CATG | 2 cut(s) | 14, 31 |
| HincII | GTYRAC | 1 cut(s) | 19 |
| HindII | GTYRAC | 1 cut(s) | 19 |
| HinfI | GANTC | 2 cut(s) | 239, 676 |
| HphI | GGTGA | 2 cut(s) | 191, 572 |
| Hpy166II | GTNNAC | 1 cut(s) | 19 |
| Hpy188I | TCNGA | 2 cut(s) | 190, 322 |
| Hpy188III | TCNNGA | 1 cut(s) | 11 |
| Hpy8I | GTNNAC | 1 cut(s) | 19 |
| Hpy99I | CGWCG | 3 cut(s) | 93, 96, 194 |
| HpyAV | CCTTC | 1 cut(s) | 454 |
| HpyCH4III | ACNGT | 1 cut(s) | 185 |
| HpyCH4IV | ACGT | 3 cut(s) | 15, 126, 145 |
| HpyCH4V | TGCA | 2 cut(s) | 514, 670 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 449 |
| HpySE526I | ACGT | 3 cut(s) | 15, 126, 145 |
| Hsp92I | GRCGYC | 1 cut(s) | 15 |
| Hsp92II | CATG | 2 cut(s) | 14, 31 |
| Kzo9I | GATC | 3 cut(s) | 151, 444, 617 |
| LmnI | GCTCC | 1 cut(s) | 440 |
| LpnPI | CCDG | 1 cut(s) | 273 |
| LweI | GCATC | 3 cut(s) | 36, 87, 165 |
| MaeII | ACGT | 3 cut(s) | 15, 126, 145 |
| MaeIII | GTNAC | 2 cut(s) | 127, 179 |
| MalI | GATC | 3 cut(s) | 153, 446, 619 |
| MboI | GATC | 3 cut(s) | 151, 444, 617 |
| MboII | GAAGA | 7 cut(s) | 428, 437, 469, 575, 607, 669, 701 |
| MfeI | CAATTG | 2 cut(s) | 403, 665 |
| MluCI | AATT | 9 cut(s) | 286, 316, 366, 403, 475, 516, 568, 602, 665 |
| MmeI | TCCRAC | 2 cut(s) | 135, 213 |
| MnlI | CCTC | 2 cut(s) | 272, 382 |
| MseI | TTAA | 3 cut(s) | 309, 498, 534 |
| MslI | CAYNNNNRTG | 1 cut(s) | 222 |
| MunI | CAATTG | 2 cut(s) | 403, 665 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 449 |
| NdeII | GATC | 3 cut(s) | 151, 444, 617 |
| NlaIII | CATG | 2 cut(s) | 14, 31 |
| NlaIV | GGNNCC | 1 cut(s) | 187 |
| NmuCI | GTSAC | 1 cut(s) | 179 |
| PagI | TCATGA | 1 cut(s) | 10 |
| PfeI | GAWTC | 2 cut(s) | 239, 676 |
| PspN4I | GGNNCC | 1 cut(s) | 187 |
| PspPI | GGNCC | 1 cut(s) | 209 |
| RsaI | GTAC | 1 cut(s) | 66 |
| RsaNI | GTAC | 1 cut(s) | 65 |
| RseI | CAYNNNNRTG | 1 cut(s) | 222 |
| SaqAI | TTAA | 3 cut(s) | 309, 498, 534 |
| Sau3AI | GATC | 3 cut(s) | 151, 444, 617 |
| Sau96I | GGNCC | 1 cut(s) | 209 |
| SetI | ASST | 6 cut(s) | 18, 129, 148, 262, 283, 424 |
| SfaNI | GCATC | 3 cut(s) | 36, 87, 165 |
| SinI | GGWCC | 1 cut(s) | 209 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 222 |
| Sse9I | AATT | 9 cut(s) | 286, 316, 366, 403, 475, 516, 568, 602, 665 |
| SsiI | CCGC | 1 cut(s) | 62 |
| TaaI | ACNGT | 1 cut(s) | 185 |
| TaiI | ACGT | 3 cut(s) | 18, 129, 148 |
| TaqI | TCGA | 5 cut(s) | 81, 88, 159, 270, 527 |
| TasI | AATT | 9 cut(s) | 286, 316, 366, 403, 475, 516, 568, 602, 665 |
| TfiI | GAWTC | 2 cut(s) | 239, 676 |
| Tru1I | TTAA | 3 cut(s) | 309, 498, 534 |
| Tru9I | TTAA | 3 cut(s) | 309, 498, 534 |
| TseFI | GTSAC | 1 cut(s) | 179 |
| Tsp45I | GTSAC | 1 cut(s) | 179 |
| TspDTI | ATGAA | 1 cut(s) | 206 |
| VpaK11BI | GGWCC | 1 cut(s) | 209 |
| XapI | RAATTY | 4 cut(s) | 286, 516, 568, 602 |
| ZraI | GACGTC | 1 cut(s) | 16 |