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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclI | AACGTT | 1 cut(s) | 126 |
| AclWI | GGATC | 1 cut(s) | 105 |
| AcsI | RAATTY | 2 cut(s) | 195, 302 |
| AcuI | CTGAAG | 1 cut(s) | 35 |
| Alw26I | GTCTC | 1 cut(s) | 231 |
| AlwI | GGATC | 1 cut(s) | 105 |
| AoxI | GGCC | 1 cut(s) | 214 |
| ApoI | RAATTY | 2 cut(s) | 195, 302 |
| AsuHPI | GGTGA | 1 cut(s) | 178 |
| BccI | CCATC | 1 cut(s) | 206 |
| BcoDI | GTCTC | 1 cut(s) | 231 |
| BmsI | GCATC | 2 cut(s) | 87, 253 |
| Bse3DI | GCAATG | 1 cut(s) | 178 |
| BseMI | GCAATG | 1 cut(s) | 178 |
| BshFI | GGCC | 1 cut(s) | 216 |
| BsmAI | GTCTC | 1 cut(s) | 231 |
| BsmI | GAATGC | 2 cut(s) | 51, 328 |
| BsnI | GGCC | 1 cut(s) | 216 |
| Bsp143I | GATC | 2 cut(s) | 97, 361 |
| BspANI | GGCC | 1 cut(s) | 216 |
| BspPI | GGATC | 1 cut(s) | 105 |
| BsrDI | GCAATG | 1 cut(s) | 178 |
| BssMI | GATC | 2 cut(s) | 97, 361 |
| Bst4CI | ACNGT | 1 cut(s) | 227 |
| BstKTI | GATC | 2 cut(s) | 100, 364 |
| BstMAI | GTCTC | 1 cut(s) | 231 |
| BstMBI | GATC | 2 cut(s) | 97, 361 |
| BsuRI | GGCC | 1 cut(s) | 216 |
| CviAII | CATG | 1 cut(s) | 28 |
| CviJI | RGCY | 2 cut(s) | 32, 216 |
| CviKI_1 | RGCY | 2 cut(s) | 32, 216 |
| DpnI | GATC | 2 cut(s) | 99, 363 |
| DpnII | GATC | 2 cut(s) | 97, 361 |
| Eco32I | GATATC | 1 cut(s) | 16 |
| Eco57I | CTGAAG | 1 cut(s) | 35 |
| EcoRV | GATATC | 1 cut(s) | 16 |
| EcoT22I | ATGCAT | 1 cut(s) | 330 |
| FaeI | CATG | 1 cut(s) | 31 |
| FaiI | YATR | 9 cut(s) | 29, 67, 147, 186, 221, 223, 300, 337, 357 |
| FatI | CATG | 1 cut(s) | 27 |
| HaeIII | GGCC | 1 cut(s) | 216 |
| Hin1II | CATG | 1 cut(s) | 31 |
| HinfI | GANTC | 2 cut(s) | 158, 342 |
| HphI | GGTGA | 1 cut(s) | 178 |
| Hpy188III | TCNNGA | 1 cut(s) | 11 |
| HpyCH4III | ACNGT | 1 cut(s) | 227 |
| HpyCH4IV | ACGT | 1 cut(s) | 126 |
| HpyCH4V | TGCA | 4 cut(s) | 78, 266, 328, 339 |
| HpySE526I | ACGT | 1 cut(s) | 126 |
| Hsp92II | CATG | 1 cut(s) | 31 |
| Kzo9I | GATC | 2 cut(s) | 97, 361 |
| LweI | GCATC | 2 cut(s) | 87, 253 |
| MaeII | ACGT | 1 cut(s) | 126 |
| MaeIII | GTNAC | 1 cut(s) | 127 |
| MalI | GATC | 2 cut(s) | 99, 363 |
| MboI | GATC | 2 cut(s) | 97, 361 |
| MboII | GAAGA | 3 cut(s) | 167, 221, 260 |
| MluCI | AATT | 3 cut(s) | 195, 302, 352 |
| MmeI | TCCRAC | 1 cut(s) | 135 |
| MnlI | CCTC | 1 cut(s) | 227 |
| Mph1103I | ATGCAT | 1 cut(s) | 330 |
| MseI | TTAA | 1 cut(s) | 323 |
| Mva1269I | GAATGC | 2 cut(s) | 51, 328 |
| NdeII | GATC | 2 cut(s) | 97, 361 |
| NlaIII | CATG | 1 cut(s) | 31 |
| NsiI | ATGCAT | 1 cut(s) | 330 |
| PctI | GAATGC | 2 cut(s) | 51, 328 |
| PfeI | GAWTC | 2 cut(s) | 158, 342 |
| Psp1406I | AACGTT | 1 cut(s) | 126 |
| SaqAI | TTAA | 1 cut(s) | 323 |
| Sau3AI | GATC | 2 cut(s) | 97, 361 |
| SetI | ASST | 1 cut(s) | 129 |
| SfaNI | GCATC | 2 cut(s) | 87, 253 |
| SgeI | CNNG | 7 cut(s) | 23, 40, 71, 127, 174, 202, 283 |
| Sse9I | AATT | 3 cut(s) | 195, 302, 352 |
| SspI | AATATT | 1 cut(s) | 279 |
| TaaI | ACNGT | 1 cut(s) | 227 |
| TaiI | ACGT | 1 cut(s) | 129 |
| TaqI | TCGA | 1 cut(s) | 72 |
| TasI | AATT | 3 cut(s) | 195, 302, 352 |
| TfiI | GAWTC | 2 cut(s) | 158, 342 |
| Tru1I | TTAA | 1 cut(s) | 323 |
| Tru9I | TTAA | 1 cut(s) | 323 |
| TspDTI | ATGAA | 3 cut(s) | 173, 261, 321 |
| XapI | RAATTY | 2 cut(s) | 195, 302 |
| Zsp2I | ATGCAT | 1 cut(s) | 330 |