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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 14 |
| AcsI | RAATTY | 1 cut(s) | 315 |
| AfaI | GTAC | 1 cut(s) | 51 |
| AgsI | TTSAA | 6 cut(s) | 41, 62, 172, 210, 307, 314 |
| AjnI | CCWGG | 1 cut(s) | 184 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 152, 184 |
| AlwI | GGATC | 1 cut(s) | 14 |
| ApoI | RAATTY | 1 cut(s) | 315 |
| AsuHPI | GGTGA | 2 cut(s) | 122, 164 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 33, 66 |
| BauI | CACGAG | 1 cut(s) | 79 |
| BbsI | GAAGAC | 2 cut(s) | 75, 359 |
| BciT130I | CCWGG | 1 cut(s) | 186 |
| BfaI | CTAG | 1 cut(s) | 192 |
| Bme1390I | CCNGG | 1 cut(s) | 186 |
| BmrFI | CCNGG | 1 cut(s) | 186 |
| BmsI | GCATC | 1 cut(s) | 145 |
| BpiI | GAAGAC | 2 cut(s) | 75, 359 |
| BpuEI | CTTGAG | 1 cut(s) | 305 |
| BsaJI | CCNNGG | 1 cut(s) | 139 |
| Bse1I | ACTGG | 1 cut(s) | 361 |
| BseBI | CCWGG | 1 cut(s) | 186 |
| BseDI | CCNNGG | 1 cut(s) | 139 |
| BseGI | GGATG | 1 cut(s) | 223 |
| BseNI | ACTGG | 1 cut(s) | 361 |
| BseRI | GAGGAG | 1 cut(s) | 389 |
| Bsp143I | GATC | 1 cut(s) | 19 |
| Bsp19I | CCATGG | 1 cut(s) | 139 |
| BspPI | GGATC | 1 cut(s) | 14 |
| BsrI | ACTGG | 1 cut(s) | 361 |
| BssECI | CCNNGG | 1 cut(s) | 139 |
| BssMI | GATC | 1 cut(s) | 19 |
| BssSI | CACGAG | 1 cut(s) | 79 |
| BssT1I | CCWWGG | 1 cut(s) | 139 |
| Bst2BI | CACGAG | 1 cut(s) | 79 |
| Bst2UI | CCWGG | 1 cut(s) | 186 |
| Bst4CI | ACNGT | 1 cut(s) | 49 |
| BstDSI | CCRYGG | 1 cut(s) | 139 |
| BstF5I | GGATG | 1 cut(s) | 223 |
| BstKTI | GATC | 1 cut(s) | 22 |
| BstMBI | GATC | 1 cut(s) | 19 |
| BstNI | CCWGG | 1 cut(s) | 186 |
| BstNSI | RCATGY | 1 cut(s) | 10 |
| BstSCI | CCNGG | 1 cut(s) | 184 |
| BstV2I | GAAGAC | 2 cut(s) | 75, 359 |
| BtgI | CCRYGG | 1 cut(s) | 139 |
| BtsCI | GGATG | 1 cut(s) | 223 |
| Csp6I | GTAC | 1 cut(s) | 50 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 45, 80 |
| CviAII | CATG | 3 cut(s) | 7, 140, 197 |
| CviJI | RGCY | 1 cut(s) | 239 |
| CviKI_1 | RGCY | 1 cut(s) | 239 |
| CviQI | GTAC | 1 cut(s) | 50 |
| DpnI | GATC | 1 cut(s) | 21 |
| DpnII | GATC | 1 cut(s) | 19 |
| Eco130I | CCWWGG | 1 cut(s) | 139 |
| EcoRII | CCWGG | 1 cut(s) | 184 |
| EcoT14I | CCWWGG | 1 cut(s) | 139 |
| ErhI | CCWWGG | 1 cut(s) | 139 |
| FaeI | CATG | 3 cut(s) | 10, 143, 200 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 223, 255 |
| FatI | CATG | 3 cut(s) | 6, 139, 196 |
| FokI | GGATG | 1 cut(s) | 230 |
| FspBI | CTAG | 1 cut(s) | 192 |
| Hin1II | CATG | 3 cut(s) | 10, 143, 200 |
| HinfI | GANTC | 1 cut(s) | 365 |
| HphI | GGTGA | 2 cut(s) | 122, 164 |
| Hpy166II | GTNNAC | 1 cut(s) | 271 |
| Hpy188I | TCNGA | 2 cut(s) | 348, 370 |
| Hpy188III | TCNNGA | 1 cut(s) | 322 |
| Hpy8I | GTNNAC | 1 cut(s) | 271 |
| HpyCH4III | ACNGT | 1 cut(s) | 49 |
| HpyCH4IV | ACGT | 1 cut(s) | 162 |
| HpyCH4V | TGCA | 2 cut(s) | 4, 10 |
| HpySE526I | ACGT | 1 cut(s) | 162 |
| Hsp92II | CATG | 3 cut(s) | 10, 143, 200 |
| Kzo9I | GATC | 1 cut(s) | 19 |
| LmnI | GCTCC | 1 cut(s) | 376 |
| LpnPI | CCDG | 3 cut(s) | 171, 198, 342 |
| LweI | GCATC | 1 cut(s) | 145 |
| MaeI | CTAG | 1 cut(s) | 192 |
| MaeII | ACGT | 1 cut(s) | 162 |
| MalI | GATC | 1 cut(s) | 21 |
| MboI | GATC | 1 cut(s) | 19 |
| MboII | GAAGA | 3 cut(s) | 80, 361, 364 |
| MfeI | CAATTG | 1 cut(s) | 57 |
| MluCI | AATT | 7 cut(s) | 15, 57, 172, 249, 307, 315, 391 |
| MlyI | GAGTC | 1 cut(s) | 374 |
| MnlI | CCTC | 3 cut(s) | 108, 239, 367 |
| MseI | TTAA | 2 cut(s) | 297, 394 |
| MslI | CAYNNNNRTG | 1 cut(s) | 150 |
| MspR9I | CCNGG | 1 cut(s) | 186 |
| MunI | CAATTG | 1 cut(s) | 57 |
| MvaI | CCWGG | 1 cut(s) | 186 |
| NcoI | CCATGG | 1 cut(s) | 139 |
| NdeII | GATC | 1 cut(s) | 19 |
| NlaIII | CATG | 3 cut(s) | 10, 143, 200 |
| NspI | RCATGY | 1 cut(s) | 10 |
| PfoI | TCCNGGA | 1 cut(s) | 184 |
| PleI | GAGTC | 1 cut(s) | 373 |
| PpsI | GAGTC | 1 cut(s) | 373 |
| Psp6I | CCWGG | 1 cut(s) | 184 |
| PspGI | CCWGG | 1 cut(s) | 184 |
| RsaI | GTAC | 1 cut(s) | 51 |
| RsaNI | GTAC | 1 cut(s) | 50 |
| RseI | CAYNNNNRTG | 1 cut(s) | 150 |
| SaqAI | TTAA | 2 cut(s) | 297, 394 |
| Sau3AI | GATC | 1 cut(s) | 19 |
| SchI | GAGTC | 1 cut(s) | 374 |
| ScrFI | CCNGG | 1 cut(s) | 186 |
| SetI | ASST | 3 cut(s) | 165, 208, 257 |
| SfaNI | GCATC | 1 cut(s) | 145 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 150 |
| SmlI | CTYRAG | 1 cut(s) | 320 |
| SmoI | CTYRAG | 1 cut(s) | 320 |
| Sse9I | AATT | 7 cut(s) | 15, 57, 172, 249, 307, 315, 391 |
| SspMI | CTAG | 1 cut(s) | 192 |
| StyD4I | CCNGG | 1 cut(s) | 184 |
| StyI | CCWWGG | 1 cut(s) | 139 |
| TaaI | ACNGT | 1 cut(s) | 49 |
| TaiI | ACGT | 1 cut(s) | 165 |
| TasI | AATT | 7 cut(s) | 15, 57, 172, 249, 307, 315, 391 |
| Tru1I | TTAA | 2 cut(s) | 297, 394 |
| Tru9I | TTAA | 2 cut(s) | 297, 394 |
| TspDTI | ATGAA | 2 cut(s) | 143, 278 |
| TspGWI | ACGGA | 1 cut(s) | 268 |
| XapI | RAATTY | 1 cut(s) | 315 |
| XceI | RCATGY | 1 cut(s) | 10 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 146 |
| XspI | CTAG | 1 cut(s) | 192 |