Rroxscaffold_4G00312510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0001043
Physical Location & Seq
Forward (+)
35503252 .. 35509854
6603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312510.1

Sequence Viewer

Length: 528 bp
ATGTTTACCATTGCCTCTGTTAGTTCTCTTCATGAAGCTATTGATCCAGAATTGAATGCTGATCTGGTGTCTTCATGCTCATGGAAAGATAATTGTTTTGTACATTTTAAAAGAGAACTTGAATGGAATGGAGGAATCTTGGGTGAGCAAATGGTTCATTCCATAACAGGAAAATTGGCTCAATGGAAGATGGTCTCATATATGGATTCTGAGAAAAATTTTGGGATTGCGAAGTTAATGTTGGAGATAAAATTTATGGGGTCAAGATTAAGCATTTATTCCAAATATCTATTTGTTTGGAAACGAAGGATTCAATTGCAAGTTAAAGGTTCAACAAAAGATCTAAAGCAATGGAAGACTTTGTCAAGATTTTCTTCACTCAATGACTCAAATTTGAGGGTGAATTCTTTCCAACCAAGGGAGTCCGATGCAGGAGCCGACCTCTTTGGGAACTTCCGGCCGGTGACTGGTGATCGGATTCCCACGGCTAGTGATCGAAGTCCCGCGAATTTGTCTTTTTACATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

175

Amino Acids

20.0

Weight (kDa)

9.27

Isoelectric Point (pI)

39.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 506
AciI CCGC 1 cut(s) 504
AclWI GGATC 1 cut(s) 38
AcoI YGGCCR 1 cut(s) 458
AcsI RAATTY 5 cut(s) 217, 251, 391, 403, 508
AfaI GTAC 1 cut(s) 102
AfiI CCNNNNNNNGG 2 cut(s) 418, 467
AgsI TTSAA 4 cut(s) 55, 122, 314, 333
AjuI GAANNNNNNNTTGG 2 cut(s) 224, 256
AluBI AGCT 1 cut(s) 38
AluI AGCT 1 cut(s) 38
Alw26I GTCTC 1 cut(s) 199
AlwI GGATC 1 cut(s) 38
AoxI GGCC 1 cut(s) 458
ApoI RAATTY 5 cut(s) 217, 251, 391, 403, 508
Asp700I GAANNNNTTC 1 cut(s) 407
AsuHPI GGTGA 4 cut(s) 155, 412, 475, 482
BbsI GAAGAC 2 cut(s) 63, 362
BccI CCATC 1 cut(s) 184
BceAI ACGGC 1 cut(s) 501
BcoDI GTCTC 1 cut(s) 199
BfaI CTAG 1 cut(s) 489
BglII AGATCT 1 cut(s) 340
BmiI GGNNCC 1 cut(s) 436
BmsI GCATC 1 cut(s) 418
BpiI GAAGAC 2 cut(s) 63, 362
BplI GAGNNNNNCTC 2 cut(s) 426, 458
BsaI GGTCTC 1 cut(s) 199
BsaJI CCNNGG 2 cut(s) 416, 483
Bsc4I CCNNNNNNNGG 2 cut(s) 418, 467
Bse118I RCCGGY 1 cut(s) 460
Bse1I ACTGG 1 cut(s) 472
Bse3DI GCAATG 2 cut(s) 9, 356
BseDI CCNNGG 2 cut(s) 416, 483
BseLI CCNNNNNNNGG 2 cut(s) 418, 467
BseMI GCAATG 2 cut(s) 9, 356
BseMII CTCAG 1 cut(s) 201
BseNI ACTGG 1 cut(s) 472
BseX3I CGGCCG 1 cut(s) 458
Bsh1236I CGCG 1 cut(s) 506
Bsh1285I CGRYCG 1 cut(s) 461
BshFI GGCC 1 cut(s) 460
BsiEI CGRYCG 1 cut(s) 461
BsiSI CCGG 2 cut(s) 457, 461
BslFI GGGAC 1 cut(s) 486
BslI CCNNNNNNNGG 2 cut(s) 418, 467
BsmAI GTCTC 1 cut(s) 199
BsmFI GGGAC 1 cut(s) 486
BsmI GAATGC 1 cut(s) 61
BsnI GGCC 1 cut(s) 460
Bso31I GGTCTC 1 cut(s) 199
Bsp1407I TGTACA 1 cut(s) 100
Bsp143I GATC 5 cut(s) 43, 61, 340, 472, 493
BspACI CCGC 1 cut(s) 504
BspANI GGCC 1 cut(s) 460
BspCNI CTCAG 1 cut(s) 202
BspFNI CGCG 1 cut(s) 506
BspHI TCATGA 1 cut(s) 31
BspLI GGNNCC 1 cut(s) 436
BspPI GGATC 1 cut(s) 38
BspTNI GGTCTC 1 cut(s) 199
BsrDI GCAATG 2 cut(s) 9, 356
BsrFI RCCGGY 1 cut(s) 460
BsrGI TGTACA 1 cut(s) 100
BsrI ACTGG 1 cut(s) 472
BssAI RCCGGY 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 416, 483
BssMI GATC 5 cut(s) 43, 61, 340, 472, 493
BssT1I CCWWGG 1 cut(s) 416
Bst6I CTCTTC 1 cut(s) 33
BstAUI TGTACA 1 cut(s) 100
BstDEI CTNAG 1 cut(s) 210
BstDSI CCRYGG 1 cut(s) 483
BstFNI CGCG 1 cut(s) 506
BstKTI GATC 5 cut(s) 46, 64, 343, 475, 496
BstMAI GTCTC 1 cut(s) 199
BstMBI GATC 5 cut(s) 43, 61, 340, 472, 493
BstMCI CGRYCG 1 cut(s) 461
BstUI CGCG 1 cut(s) 506
BstV2I GAAGAC 2 cut(s) 63, 362
BstX2I RGATCY 1 cut(s) 340
BstYI RGATCY 1 cut(s) 340
BstZI CGGCCG 1 cut(s) 458
BsuRI GGCC 1 cut(s) 460
BtgI CCRYGG 1 cut(s) 483
CciI TCATGA 1 cut(s) 31
Cfr10I RCCGGY 1 cut(s) 460
Csp6I GTAC 1 cut(s) 101
CviAII CATG 3 cut(s) 32, 75, 81
CviJI RGCY 5 cut(s) 38, 179, 437, 460, 488
CviKI_1 RGCY 5 cut(s) 38, 179, 437, 460, 488
CviQI GTAC 1 cut(s) 101
DdeI CTNAG 1 cut(s) 210
DpnI GATC 5 cut(s) 45, 63, 342, 474, 495
DpnII GATC 5 cut(s) 43, 61, 340, 472, 493
DraI TTTAAA 1 cut(s) 109
EaeI YGGCCR 1 cut(s) 458
EagI CGGCCG 1 cut(s) 458
Eam1104I CTCTTC 1 cut(s) 33
EarI CTCTTC 1 cut(s) 33
EclXI CGGCCG 1 cut(s) 458
Eco130I CCWWGG 1 cut(s) 416
Eco31I GGTCTC 1 cut(s) 199
Eco52I CGGCCG 1 cut(s) 458
EcoRI GAATTC 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 416
ErhI CCWWGG 1 cut(s) 416
FaeI CATG 3 cut(s) 35, 78, 84
FaiI YATR 8 cut(s) 33, 76, 82, 164, 199, 201, 203, 257
FalI AAGNNNNNCTT 2 cut(s) 358, 390
FaqI GGGAC 1 cut(s) 486
FatI CATG 3 cut(s) 31, 74, 80
FauI CCCGC 1 cut(s) 511
FspBI CTAG 1 cut(s) 489
HaeIII GGCC 1 cut(s) 460
HapII CCGG 2 cut(s) 457, 461
Hin1II CATG 3 cut(s) 35, 78, 84
HinfI GANTC 6 cut(s) 135, 206, 310, 386, 422, 478
HpaII CCGG 2 cut(s) 457, 461
HphI GGTGA 4 cut(s) 155, 412, 475, 482
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 3 cut(s) 211, 427, 477
Hpy188III TCNNGA 4 cut(s) 32, 47, 264, 366
Hpy8I GTNNAC 1 cut(s) 6
HpyAV CCTTC 1 cut(s) 300
HpyCH4V TGCA 2 cut(s) 319, 431
HpyF3I CTNAG 1 cut(s) 210
Hsp92II CATG 3 cut(s) 35, 78, 84
Kzo9I GATC 5 cut(s) 43, 61, 340, 472, 493
LmnI GCTCC 1 cut(s) 434
LpnPI CCDG 7 cut(s) 50, 60, 153, 417, 453, 470, 474
LweI GCATC 1 cut(s) 418
MaeI CTAG 1 cut(s) 489
MaeIII GTNAC 1 cut(s) 463
MalI GATC 5 cut(s) 45, 63, 342, 474, 495
MboI GATC 5 cut(s) 43, 61, 340, 472, 493
MboII GAAGA 5 cut(s) 20, 63, 199, 366, 367
MfeI CAATTG 1 cut(s) 314
MflI RGATCY 1 cut(s) 340
MluCI AATT 9 cut(s) 50, 91, 173, 217, 251, 314, 391, 403, 508
MlyI GAGTC 2 cut(s) 380, 431
MmeI TCCRAC 2 cut(s) 222, 436
MnlI CCTC 4 cut(s) 25, 125, 390, 452
MroXI GAANNNNTTC 1 cut(s) 407
MseI TTAA 5 cut(s) 108, 236, 269, 324, 526
MslI CAYNNNNRTG 1 cut(s) 79
MspI CCGG 2 cut(s) 457, 461
MunI CAATTG 1 cut(s) 314
Mva1269I GAATGC 1 cut(s) 61
MvnI CGCG 1 cut(s) 506
NdeII GATC 5 cut(s) 43, 61, 340, 472, 493
NlaIII CATG 3 cut(s) 35, 78, 84
NlaIV GGNNCC 1 cut(s) 436
NmuCI GTSAC 1 cut(s) 463
PagI TCATGA 1 cut(s) 31
PctI GAATGC 1 cut(s) 61
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 4 cut(s) 135, 206, 310, 478
PflFI GACNNNGTC 1 cut(s) 361
PleI GAGTC 2 cut(s) 380, 430
PpsI GAGTC 2 cut(s) 380, 430
PspN4I GGNNCC 1 cut(s) 436
PsuI RGATCY 1 cut(s) 340
PsyI GACNNNGTC 1 cut(s) 361
RsaI GTAC 1 cut(s) 102
RsaNI GTAC 1 cut(s) 101
RseI CAYNNNNRTG 1 cut(s) 79
SaqAI TTAA 5 cut(s) 108, 236, 269, 324, 526
Sau3AI GATC 5 cut(s) 43, 61, 340, 472, 493
SchI GAGTC 2 cut(s) 380, 431
SetI ASST 3 cut(s) 40, 331, 444
SfaNI GCATC 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 79
Sse9I AATT 9 cut(s) 50, 91, 173, 217, 251, 314, 391, 403, 508
SsiI CCGC 1 cut(s) 504
SspMI CTAG 1 cut(s) 489
StyI CCWWGG 1 cut(s) 416
TaqI TCGA 1 cut(s) 496
TasI AATT 9 cut(s) 50, 91, 173, 217, 251, 314, 391, 403, 508
TatI WGTACW 1 cut(s) 100
TfiI GAWTC 4 cut(s) 135, 206, 310, 478
Tru1I TTAA 5 cut(s) 108, 236, 269, 324, 526
Tru9I TTAA 5 cut(s) 108, 236, 269, 324, 526
TseFI GTSAC 1 cut(s) 463
Tsp45I GTSAC 1 cut(s) 463
TspDTI ATGAA 4 cut(s) 20, 48, 63, 146
Tth111I GACNNNGTC 1 cut(s) 361
XapI RAATTY 5 cut(s) 217, 251, 391, 403, 508
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 1 cut(s) 489
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.